Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2

Autores
Sulbarán, Maria Z.; Di Lello, Federico Alejandro; Sulbarán, Yoneira; Cosson, Clarisa; Loureiro, Carmen; Rangel, Héctor R.; Cantaloube, Jean F.; Campos, Rodolfo Hector; Moratorio, Gonzalo; Cristina, Juan; Pujol, Flor H.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The subtype diversity of the hepatitis C virus (HCV) genotypes is unknown in Venezuela. Methodology/Principal Findings: Partial sequencing of the NS5B region was performed in 310 isolates circulating in patients from 1995 to 2007. In the samples collected between 2005 and 2007, HCV genotype 1 (G1) was the most common genotype (63%), composed as expected of mainly G1a and G1b. G2 was the second most common genotype (33%), being G2a almost absent and G2j the most frequent subtype. Sequence analysis of the core region confirmed the subtype assignment performed within the NS5b region in 63 isolates. The complete genome sequence of G2j was obtained. G2j has been described in France, Canada and Burkina Fasso, but it was not found in Martinique, where several subtypes of G2 circulate in the general population. Bayesian coalescence analysis indicated a most recent common ancestor (MRCA) of G2j around 1785, before the introduction of G1b (1869) and G1a (1922). While HCV G1a and G1b experienced a growth reduction since 1990, coincident with the time when blood testing was implemented in Venezuela, HCV G2j did not seem to reach growth equilibrium during this period. Conclusions/Significance: Assuming the introduction of G2j from Africa during the slave trade, the high frequency of G2j found in Venezuela could suggest: 1- the introduction of African ethnic groups different from the ones introduced to Martinique or 2- the occurrence of a founder effect. This study represents an in-depth analysis of the subtype diversity of HCV in Venezuela, which is still unexplored in the Americas and deserves further studies.
Fil: Sulbarán, Maria Z.. Instituto Venezolano de Investigaciones Cientificas; Venezuela
Fil: Di Lello, Federico Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina
Fil: Sulbarán, Yoneira. Instituto Venezolano de Investigaciones Cientificas; Venezuela
Fil: Cosson, Clarisa. Instituto Venezolano de Investigaciones Cientificas; Venezuela
Fil: Loureiro, Carmen. Instituto Venezolano de Investigaciones Cientificas; Venezuela
Fil: Rangel, Héctor R.. Instituto Venezolano de Investigaciones Cientificas; Venezuela
Fil: Cantaloube, Jean F.. Etablissement Français du Sang Alpes-Méditerranée. Unité Emergence et Co-évolution virale; Francia
Fil: Campos, Rodolfo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina
Fil: Moratorio, Gonzalo. Universidad de la Republica; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Cristina, Juan. Universidad de la Republica; Uruguay
Fil: Pujol, Flor H.. Instituto Venezolano de Investigaciones Cientificas; Venezuela
Materia
VIRAL EVOLUTION
VIRAL EPIDEMIOLOGY
HEPATITIS C VIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14200
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14200
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2Sulbarán, Maria Z.Di Lello, Federico AlejandroSulbarán, YoneiraCosson, ClarisaLoureiro, CarmenRangel, Héctor R.Cantaloube, Jean F.Campos, Rodolfo HectorMoratorio, GonzaloCristina, JuanPujol, Flor H.VIRAL EVOLUTIONVIRAL EPIDEMIOLOGYHEPATITIS C VIRUShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: The subtype diversity of the hepatitis C virus (HCV) genotypes is unknown in Venezuela. Methodology/Principal Findings: Partial sequencing of the NS5B region was performed in 310 isolates circulating in patients from 1995 to 2007. In the samples collected between 2005 and 2007, HCV genotype 1 (G1) was the most common genotype (63%), composed as expected of mainly G1a and G1b. G2 was the second most common genotype (33%), being G2a almost absent and G2j the most frequent subtype. Sequence analysis of the core region confirmed the subtype assignment performed within the NS5b region in 63 isolates. The complete genome sequence of G2j was obtained. G2j has been described in France, Canada and Burkina Fasso, but it was not found in Martinique, where several subtypes of G2 circulate in the general population. Bayesian coalescence analysis indicated a most recent common ancestor (MRCA) of G2j around 1785, before the introduction of G1b (1869) and G1a (1922). While HCV G1a and G1b experienced a growth reduction since 1990, coincident with the time when blood testing was implemented in Venezuela, HCV G2j did not seem to reach growth equilibrium during this period. Conclusions/Significance: Assuming the introduction of G2j from Africa during the slave trade, the high frequency of G2j found in Venezuela could suggest: 1- the introduction of African ethnic groups different from the ones introduced to Martinique or 2- the occurrence of a founder effect. This study represents an in-depth analysis of the subtype diversity of HCV in Venezuela, which is still unexplored in the Americas and deserves further studies.Fil: Sulbarán, Maria Z.. Instituto Venezolano de Investigaciones Cientificas; VenezuelaFil: Di Lello, Federico Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; ArgentinaFil: Sulbarán, Yoneira. Instituto Venezolano de Investigaciones Cientificas; VenezuelaFil: Cosson, Clarisa. Instituto Venezolano de Investigaciones Cientificas; VenezuelaFil: Loureiro, Carmen. Instituto Venezolano de Investigaciones Cientificas; VenezuelaFil: Rangel, Héctor R.. Instituto Venezolano de Investigaciones Cientificas; VenezuelaFil: Cantaloube, Jean F.. Etablissement Français du Sang Alpes-Méditerranée. Unité Emergence et Co-évolution virale; FranciaFil: Campos, Rodolfo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; ArgentinaFil: Moratorio, Gonzalo. Universidad de la Republica; Uruguay. Instituto Pasteur de Montevideo; UruguayFil: Cristina, Juan. Universidad de la Republica; UruguayFil: Pujol, Flor H.. Instituto Venezolano de Investigaciones Cientificas; VenezuelaPublic Library Of Science2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14200Sulbarán, Maria Z.; Di Lello, Federico Alejandro; Sulbarán, Yoneira; Cosson, Clarisa; Loureiro, Carmen; et al.; Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2; Public Library Of Science; Plos One; 5; 12; 12-2010; 5-143151932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0014315info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0014315info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:55Zoai:ri.conicet.gov.ar:11336/14200instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:55.622CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2
title Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2
spellingShingle Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2
Sulbarán, Maria Z.
VIRAL EVOLUTION
VIRAL EPIDEMIOLOGY
HEPATITIS C VIRUS
title_short Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2
title_full Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2
title_fullStr Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2
title_full_unstemmed Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2
title_sort Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2
dc.creator.none.fl_str_mv Sulbarán, Maria Z.
Di Lello, Federico Alejandro
Sulbarán, Yoneira
Cosson, Clarisa
Loureiro, Carmen
Rangel, Héctor R.
Cantaloube, Jean F.
Campos, Rodolfo Hector
Moratorio, Gonzalo
Cristina, Juan
Pujol, Flor H.
author Sulbarán, Maria Z.
author_facet Sulbarán, Maria Z.
Di Lello, Federico Alejandro
Sulbarán, Yoneira
Cosson, Clarisa
Loureiro, Carmen
Rangel, Héctor R.
Cantaloube, Jean F.
Campos, Rodolfo Hector
Moratorio, Gonzalo
Cristina, Juan
Pujol, Flor H.
author_role author
author2 Di Lello, Federico Alejandro
Sulbarán, Yoneira
Cosson, Clarisa
Loureiro, Carmen
Rangel, Héctor R.
Cantaloube, Jean F.
Campos, Rodolfo Hector
Moratorio, Gonzalo
Cristina, Juan
Pujol, Flor H.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv VIRAL EVOLUTION
VIRAL EPIDEMIOLOGY
HEPATITIS C VIRUS
topic VIRAL EVOLUTION
VIRAL EPIDEMIOLOGY
HEPATITIS C VIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: The subtype diversity of the hepatitis C virus (HCV) genotypes is unknown in Venezuela. Methodology/Principal Findings: Partial sequencing of the NS5B region was performed in 310 isolates circulating in patients from 1995 to 2007. In the samples collected between 2005 and 2007, HCV genotype 1 (G1) was the most common genotype (63%), composed as expected of mainly G1a and G1b. G2 was the second most common genotype (33%), being G2a almost absent and G2j the most frequent subtype. Sequence analysis of the core region confirmed the subtype assignment performed within the NS5b region in 63 isolates. The complete genome sequence of G2j was obtained. G2j has been described in France, Canada and Burkina Fasso, but it was not found in Martinique, where several subtypes of G2 circulate in the general population. Bayesian coalescence analysis indicated a most recent common ancestor (MRCA) of G2j around 1785, before the introduction of G1b (1869) and G1a (1922). While HCV G1a and G1b experienced a growth reduction since 1990, coincident with the time when blood testing was implemented in Venezuela, HCV G2j did not seem to reach growth equilibrium during this period. Conclusions/Significance: Assuming the introduction of G2j from Africa during the slave trade, the high frequency of G2j found in Venezuela could suggest: 1- the introduction of African ethnic groups different from the ones introduced to Martinique or 2- the occurrence of a founder effect. This study represents an in-depth analysis of the subtype diversity of HCV in Venezuela, which is still unexplored in the Americas and deserves further studies.
Fil: Sulbarán, Maria Z.. Instituto Venezolano de Investigaciones Cientificas; Venezuela
Fil: Di Lello, Federico Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina
Fil: Sulbarán, Yoneira. Instituto Venezolano de Investigaciones Cientificas; Venezuela
Fil: Cosson, Clarisa. Instituto Venezolano de Investigaciones Cientificas; Venezuela
Fil: Loureiro, Carmen. Instituto Venezolano de Investigaciones Cientificas; Venezuela
Fil: Rangel, Héctor R.. Instituto Venezolano de Investigaciones Cientificas; Venezuela
Fil: Cantaloube, Jean F.. Etablissement Français du Sang Alpes-Méditerranée. Unité Emergence et Co-évolution virale; Francia
Fil: Campos, Rodolfo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina
Fil: Moratorio, Gonzalo. Universidad de la Republica; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Cristina, Juan. Universidad de la Republica; Uruguay
Fil: Pujol, Flor H.. Instituto Venezolano de Investigaciones Cientificas; Venezuela
description Background: The subtype diversity of the hepatitis C virus (HCV) genotypes is unknown in Venezuela. Methodology/Principal Findings: Partial sequencing of the NS5B region was performed in 310 isolates circulating in patients from 1995 to 2007. In the samples collected between 2005 and 2007, HCV genotype 1 (G1) was the most common genotype (63%), composed as expected of mainly G1a and G1b. G2 was the second most common genotype (33%), being G2a almost absent and G2j the most frequent subtype. Sequence analysis of the core region confirmed the subtype assignment performed within the NS5b region in 63 isolates. The complete genome sequence of G2j was obtained. G2j has been described in France, Canada and Burkina Fasso, but it was not found in Martinique, where several subtypes of G2 circulate in the general population. Bayesian coalescence analysis indicated a most recent common ancestor (MRCA) of G2j around 1785, before the introduction of G1b (1869) and G1a (1922). While HCV G1a and G1b experienced a growth reduction since 1990, coincident with the time when blood testing was implemented in Venezuela, HCV G2j did not seem to reach growth equilibrium during this period. Conclusions/Significance: Assuming the introduction of G2j from Africa during the slave trade, the high frequency of G2j found in Venezuela could suggest: 1- the introduction of African ethnic groups different from the ones introduced to Martinique or 2- the occurrence of a founder effect. This study represents an in-depth analysis of the subtype diversity of HCV in Venezuela, which is still unexplored in the Americas and deserves further studies.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14200
Sulbarán, Maria Z.; Di Lello, Federico Alejandro; Sulbarán, Yoneira; Cosson, Clarisa; Loureiro, Carmen; et al.; Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2; Public Library Of Science; Plos One; 5; 12; 12-2010; 5-14315
1932-6203
url http://hdl.handle.net/11336/14200
identifier_str_mv Sulbarán, Maria Z.; Di Lello, Federico Alejandro; Sulbarán, Yoneira; Cosson, Clarisa; Loureiro, Carmen; et al.; Genetic history of Hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2; Public Library Of Science; Plos One; 5; 12; 12-2010; 5-14315
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0014315
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0014315
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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