Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves

Autores
Di Lello, Federico Alejandro; Neukam, Karin; Parra Sanchez, Manuel; Plaza, Zulema; Soriano, Vicente; Cifuentes, Celia; Mira, José Antonio; Poveda, Eva; Pineda, Juan Antonio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hepatitis C virus genotype 4 (HCV-4) is highly prevalent in Spain, but the information on the molecular characterization of HCV-4 in this region is scarce. Due to this, the molecular characteristics and the evolution of HCV-4 infection in Seville were analyzed (Southern Spain) and compared them with samples from Madrid. HCV genotype was determined by LIPA 2.0 assay and confirmed by sequence analysis of NS5B. Phylogenetic tree was estimated by MEGA 5.10. Bayesian coalescent-based methods were used to estimate the substitution rate and the age of the most recent common ancestor (MRCA). In the phylogenetic analysis of 50 NS5B HCV-4 from Seville and 11 from Madrid, 2 clusters were distinguished: The first cluster (HCV-4a) included 48% of the sequences from Seville and 9% of sequences from Madrid. The second cluster included the remaining sequences belonging to HCV-4d. The mean estimated substitution rate was 2.39 × 10−3 for HCV-4a and 1.81 × 10−3 for HCV-4d for Seville and 2.32 × 10−3 for HCV-4d from Madrid. The date for MRCA was estimated to be around 1981–1984 for HCV-4 from Seville. The dates for MRCA were dated before the recent flow of immigration in Spain. Therefore, the results presented in this study argues against the possibility of a foreign introduction of the HCV-4 from other regions with high prevalence, at least during the last two, decades in which there was a great flow of immigrants. Additionally, an unusual high prevalence of subtype 4a was observed in Seville.
Fil: Di Lello, Federico Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina; Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Neukam, Karin. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Parra Sanchez, Manuel. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Plaza, Zulema. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Soriano, Vicente. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Cifuentes, Celia. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Mira, José Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Poveda, Eva. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Pineda, Juan Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Materia
Hcv
Diversity
Phylogeny
Beast
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1991
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1991
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network_name_str CONICET Digital (CONICET)
spelling Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration wavesDi Lello, Federico AlejandroNeukam, KarinParra Sanchez, ManuelPlaza, ZulemaSoriano, VicenteCifuentes, CeliaMira, José AntonioPoveda, EvaPineda, Juan AntonioHcvDiversityPhylogenyBeasthttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hepatitis C virus genotype 4 (HCV-4) is highly prevalent in Spain, but the information on the molecular characterization of HCV-4 in this region is scarce. Due to this, the molecular characteristics and the evolution of HCV-4 infection in Seville were analyzed (Southern Spain) and compared them with samples from Madrid. HCV genotype was determined by LIPA 2.0 assay and confirmed by sequence analysis of NS5B. Phylogenetic tree was estimated by MEGA 5.10. Bayesian coalescent-based methods were used to estimate the substitution rate and the age of the most recent common ancestor (MRCA). In the phylogenetic analysis of 50 NS5B HCV-4 from Seville and 11 from Madrid, 2 clusters were distinguished: The first cluster (HCV-4a) included 48% of the sequences from Seville and 9% of sequences from Madrid. The second cluster included the remaining sequences belonging to HCV-4d. The mean estimated substitution rate was 2.39 × 10−3 for HCV-4a and 1.81 × 10−3 for HCV-4d for Seville and 2.32 × 10−3 for HCV-4d from Madrid. The date for MRCA was estimated to be around 1981–1984 for HCV-4 from Seville. The dates for MRCA were dated before the recent flow of immigration in Spain. Therefore, the results presented in this study argues against the possibility of a foreign introduction of the HCV-4 from other regions with high prevalence, at least during the last two, decades in which there was a great flow of immigrants. Additionally, an unusual high prevalence of subtype 4a was observed in Seville.Fil: Di Lello, Federico Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina; Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Fil: Neukam, Karin. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Fil: Parra Sanchez, Manuel. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Fil: Plaza, Zulema. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;Fil: Soriano, Vicente. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;Fil: Cifuentes, Celia. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Fil: Mira, José Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Fil: Poveda, Eva. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;Fil: Pineda, Juan Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Wiley2013-07-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1991Di Lello, Federico Alejandro; Neukam, Karin; Parra Sanchez, Manuel; Plaza, Zulema; Soriano, Vicente; et al.; Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves; Wiley; Journal of Medical Virology; 85; 10; 16-7-2013; 1734-17400146-6615enginfo:eu-repo/semantics/reference es info:eu-repo/semantics/reference/pmid/23861220info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jmv.23657/abstract;jsessionid=90A3F350ED36826133985E0FB7BB67F9.f02t01?systemMessage=Wiley+Online+Library+and+related+systems+will+have+3+hours+of+downtime+on+Saturday+12th+September+2015+from+10%3A00-13%3A00+BST+%2F+05%3A00-08%3A00+EDT+%2F+17%3A00-20%3A00+SGT+for+essential+maintenance.++Apologies+for+the+inconvenience.info:eu-repo/semantics/altIdentifier/doi/DOI:10.1002/jmv.23657info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:47Zoai:ri.conicet.gov.ar:11336/1991instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:48.209CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves
title Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves
spellingShingle Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves
Di Lello, Federico Alejandro
Hcv
Diversity
Phylogeny
Beast
title_short Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves
title_full Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves
title_fullStr Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves
title_full_unstemmed Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves
title_sort Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves
dc.creator.none.fl_str_mv Di Lello, Federico Alejandro
Neukam, Karin
Parra Sanchez, Manuel
Plaza, Zulema
Soriano, Vicente
Cifuentes, Celia
Mira, José Antonio
Poveda, Eva
Pineda, Juan Antonio
author Di Lello, Federico Alejandro
author_facet Di Lello, Federico Alejandro
Neukam, Karin
Parra Sanchez, Manuel
Plaza, Zulema
Soriano, Vicente
Cifuentes, Celia
Mira, José Antonio
Poveda, Eva
Pineda, Juan Antonio
author_role author
author2 Neukam, Karin
Parra Sanchez, Manuel
Plaza, Zulema
Soriano, Vicente
Cifuentes, Celia
Mira, José Antonio
Poveda, Eva
Pineda, Juan Antonio
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hcv
Diversity
Phylogeny
Beast
topic Hcv
Diversity
Phylogeny
Beast
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hepatitis C virus genotype 4 (HCV-4) is highly prevalent in Spain, but the information on the molecular characterization of HCV-4 in this region is scarce. Due to this, the molecular characteristics and the evolution of HCV-4 infection in Seville were analyzed (Southern Spain) and compared them with samples from Madrid. HCV genotype was determined by LIPA 2.0 assay and confirmed by sequence analysis of NS5B. Phylogenetic tree was estimated by MEGA 5.10. Bayesian coalescent-based methods were used to estimate the substitution rate and the age of the most recent common ancestor (MRCA). In the phylogenetic analysis of 50 NS5B HCV-4 from Seville and 11 from Madrid, 2 clusters were distinguished: The first cluster (HCV-4a) included 48% of the sequences from Seville and 9% of sequences from Madrid. The second cluster included the remaining sequences belonging to HCV-4d. The mean estimated substitution rate was 2.39 × 10−3 for HCV-4a and 1.81 × 10−3 for HCV-4d for Seville and 2.32 × 10−3 for HCV-4d from Madrid. The date for MRCA was estimated to be around 1981–1984 for HCV-4 from Seville. The dates for MRCA were dated before the recent flow of immigration in Spain. Therefore, the results presented in this study argues against the possibility of a foreign introduction of the HCV-4 from other regions with high prevalence, at least during the last two, decades in which there was a great flow of immigrants. Additionally, an unusual high prevalence of subtype 4a was observed in Seville.
Fil: Di Lello, Federico Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina; Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Neukam, Karin. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Parra Sanchez, Manuel. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Plaza, Zulema. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Soriano, Vicente. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Cifuentes, Celia. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Mira, José Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Poveda, Eva. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Pineda, Juan Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
description Hepatitis C virus genotype 4 (HCV-4) is highly prevalent in Spain, but the information on the molecular characterization of HCV-4 in this region is scarce. Due to this, the molecular characteristics and the evolution of HCV-4 infection in Seville were analyzed (Southern Spain) and compared them with samples from Madrid. HCV genotype was determined by LIPA 2.0 assay and confirmed by sequence analysis of NS5B. Phylogenetic tree was estimated by MEGA 5.10. Bayesian coalescent-based methods were used to estimate the substitution rate and the age of the most recent common ancestor (MRCA). In the phylogenetic analysis of 50 NS5B HCV-4 from Seville and 11 from Madrid, 2 clusters were distinguished: The first cluster (HCV-4a) included 48% of the sequences from Seville and 9% of sequences from Madrid. The second cluster included the remaining sequences belonging to HCV-4d. The mean estimated substitution rate was 2.39 × 10−3 for HCV-4a and 1.81 × 10−3 for HCV-4d for Seville and 2.32 × 10−3 for HCV-4d from Madrid. The date for MRCA was estimated to be around 1981–1984 for HCV-4 from Seville. The dates for MRCA were dated before the recent flow of immigration in Spain. Therefore, the results presented in this study argues against the possibility of a foreign introduction of the HCV-4 from other regions with high prevalence, at least during the last two, decades in which there was a great flow of immigrants. Additionally, an unusual high prevalence of subtype 4a was observed in Seville.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1991
Di Lello, Federico Alejandro; Neukam, Karin; Parra Sanchez, Manuel; Plaza, Zulema; Soriano, Vicente; et al.; Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves; Wiley; Journal of Medical Virology; 85; 10; 16-7-2013; 1734-1740
0146-6615
url http://hdl.handle.net/11336/1991
identifier_str_mv Di Lello, Federico Alejandro; Neukam, Karin; Parra Sanchez, Manuel; Plaza, Zulema; Soriano, Vicente; et al.; Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves; Wiley; Journal of Medical Virology; 85; 10; 16-7-2013; 1734-1740
0146-6615
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/reference es info:eu-repo/semantics/reference/pmid/23861220
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jmv.23657/abstract;jsessionid=90A3F350ED36826133985E0FB7BB67F9.f02t01?systemMessage=Wiley+Online+Library+and+related+systems+will+have+3+hours+of+downtime+on+Saturday+12th+September+2015+from+10%3A00-13%3A00+BST+%2F+05%3A00-08%3A00+EDT+%2F+17%3A00-20%3A00+SGT+for+essential+maintenance.++Apologies+for+the+inconvenience.
info:eu-repo/semantics/altIdentifier/doi/DOI:10.1002/jmv.23657
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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