Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves
- Autores
- Di Lello, Federico Alejandro; Neukam, Karin; Parra Sanchez, Manuel; Plaza, Zulema; Soriano, Vicente; Cifuentes, Celia; Mira, José Antonio; Poveda, Eva; Pineda, Juan Antonio
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Hepatitis C virus genotype 4 (HCV-4) is highly prevalent in Spain, but the information on the molecular characterization of HCV-4 in this region is scarce. Due to this, the molecular characteristics and the evolution of HCV-4 infection in Seville were analyzed (Southern Spain) and compared them with samples from Madrid. HCV genotype was determined by LIPA 2.0 assay and confirmed by sequence analysis of NS5B. Phylogenetic tree was estimated by MEGA 5.10. Bayesian coalescent-based methods were used to estimate the substitution rate and the age of the most recent common ancestor (MRCA). In the phylogenetic analysis of 50 NS5B HCV-4 from Seville and 11 from Madrid, 2 clusters were distinguished: The first cluster (HCV-4a) included 48% of the sequences from Seville and 9% of sequences from Madrid. The second cluster included the remaining sequences belonging to HCV-4d. The mean estimated substitution rate was 2.39 × 10−3 for HCV-4a and 1.81 × 10−3 for HCV-4d for Seville and 2.32 × 10−3 for HCV-4d from Madrid. The date for MRCA was estimated to be around 1981–1984 for HCV-4 from Seville. The dates for MRCA were dated before the recent flow of immigration in Spain. Therefore, the results presented in this study argues against the possibility of a foreign introduction of the HCV-4 from other regions with high prevalence, at least during the last two, decades in which there was a great flow of immigrants. Additionally, an unusual high prevalence of subtype 4a was observed in Seville.
Fil: Di Lello, Federico Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina; Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Neukam, Karin. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Parra Sanchez, Manuel. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Plaza, Zulema. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Soriano, Vicente. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Cifuentes, Celia. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Mira, José Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Poveda, Eva. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Pineda, Juan Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España; - Materia
-
Hcv
Diversity
Phylogeny
Beast - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/1991
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Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration wavesDi Lello, Federico AlejandroNeukam, KarinParra Sanchez, ManuelPlaza, ZulemaSoriano, VicenteCifuentes, CeliaMira, José AntonioPoveda, EvaPineda, Juan AntonioHcvDiversityPhylogenyBeasthttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hepatitis C virus genotype 4 (HCV-4) is highly prevalent in Spain, but the information on the molecular characterization of HCV-4 in this region is scarce. Due to this, the molecular characteristics and the evolution of HCV-4 infection in Seville were analyzed (Southern Spain) and compared them with samples from Madrid. HCV genotype was determined by LIPA 2.0 assay and confirmed by sequence analysis of NS5B. Phylogenetic tree was estimated by MEGA 5.10. Bayesian coalescent-based methods were used to estimate the substitution rate and the age of the most recent common ancestor (MRCA). In the phylogenetic analysis of 50 NS5B HCV-4 from Seville and 11 from Madrid, 2 clusters were distinguished: The first cluster (HCV-4a) included 48% of the sequences from Seville and 9% of sequences from Madrid. The second cluster included the remaining sequences belonging to HCV-4d. The mean estimated substitution rate was 2.39 × 10−3 for HCV-4a and 1.81 × 10−3 for HCV-4d for Seville and 2.32 × 10−3 for HCV-4d from Madrid. The date for MRCA was estimated to be around 1981–1984 for HCV-4 from Seville. The dates for MRCA were dated before the recent flow of immigration in Spain. Therefore, the results presented in this study argues against the possibility of a foreign introduction of the HCV-4 from other regions with high prevalence, at least during the last two, decades in which there was a great flow of immigrants. Additionally, an unusual high prevalence of subtype 4a was observed in Seville.Fil: Di Lello, Federico Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina; Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Fil: Neukam, Karin. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Fil: Parra Sanchez, Manuel. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Fil: Plaza, Zulema. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;Fil: Soriano, Vicente. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;Fil: Cifuentes, Celia. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Fil: Mira, José Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Fil: Poveda, Eva. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;Fil: Pineda, Juan Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;Wiley2013-07-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1991Di Lello, Federico Alejandro; Neukam, Karin; Parra Sanchez, Manuel; Plaza, Zulema; Soriano, Vicente; et al.; Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves; Wiley; Journal of Medical Virology; 85; 10; 16-7-2013; 1734-17400146-6615enginfo:eu-repo/semantics/reference es info:eu-repo/semantics/reference/pmid/23861220info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jmv.23657/abstract;jsessionid=90A3F350ED36826133985E0FB7BB67F9.f02t01?systemMessage=Wiley+Online+Library+and+related+systems+will+have+3+hours+of+downtime+on+Saturday+12th+September+2015+from+10%3A00-13%3A00+BST+%2F+05%3A00-08%3A00+EDT+%2F+17%3A00-20%3A00+SGT+for+essential+maintenance.++Apologies+for+the+inconvenience.info:eu-repo/semantics/altIdentifier/doi/DOI:10.1002/jmv.23657info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:58Zoai:ri.conicet.gov.ar:11336/1991instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:59.104CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves |
title |
Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves |
spellingShingle |
Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves Di Lello, Federico Alejandro Hcv Diversity Phylogeny Beast |
title_short |
Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves |
title_full |
Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves |
title_fullStr |
Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves |
title_full_unstemmed |
Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves |
title_sort |
Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves |
dc.creator.none.fl_str_mv |
Di Lello, Federico Alejandro Neukam, Karin Parra Sanchez, Manuel Plaza, Zulema Soriano, Vicente Cifuentes, Celia Mira, José Antonio Poveda, Eva Pineda, Juan Antonio |
author |
Di Lello, Federico Alejandro |
author_facet |
Di Lello, Federico Alejandro Neukam, Karin Parra Sanchez, Manuel Plaza, Zulema Soriano, Vicente Cifuentes, Celia Mira, José Antonio Poveda, Eva Pineda, Juan Antonio |
author_role |
author |
author2 |
Neukam, Karin Parra Sanchez, Manuel Plaza, Zulema Soriano, Vicente Cifuentes, Celia Mira, José Antonio Poveda, Eva Pineda, Juan Antonio |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Hcv Diversity Phylogeny Beast |
topic |
Hcv Diversity Phylogeny Beast |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Hepatitis C virus genotype 4 (HCV-4) is highly prevalent in Spain, but the information on the molecular characterization of HCV-4 in this region is scarce. Due to this, the molecular characteristics and the evolution of HCV-4 infection in Seville were analyzed (Southern Spain) and compared them with samples from Madrid. HCV genotype was determined by LIPA 2.0 assay and confirmed by sequence analysis of NS5B. Phylogenetic tree was estimated by MEGA 5.10. Bayesian coalescent-based methods were used to estimate the substitution rate and the age of the most recent common ancestor (MRCA). In the phylogenetic analysis of 50 NS5B HCV-4 from Seville and 11 from Madrid, 2 clusters were distinguished: The first cluster (HCV-4a) included 48% of the sequences from Seville and 9% of sequences from Madrid. The second cluster included the remaining sequences belonging to HCV-4d. The mean estimated substitution rate was 2.39 × 10−3 for HCV-4a and 1.81 × 10−3 for HCV-4d for Seville and 2.32 × 10−3 for HCV-4d from Madrid. The date for MRCA was estimated to be around 1981–1984 for HCV-4 from Seville. The dates for MRCA were dated before the recent flow of immigration in Spain. Therefore, the results presented in this study argues against the possibility of a foreign introduction of the HCV-4 from other regions with high prevalence, at least during the last two, decades in which there was a great flow of immigrants. Additionally, an unusual high prevalence of subtype 4a was observed in Seville. Fil: Di Lello, Federico Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina; Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España; Fil: Neukam, Karin. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España; Fil: Parra Sanchez, Manuel. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España; Fil: Plaza, Zulema. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España; Fil: Soriano, Vicente. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España; Fil: Cifuentes, Celia. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España; Fil: Mira, José Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España; Fil: Poveda, Eva. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España; Fil: Pineda, Juan Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España; |
description |
Hepatitis C virus genotype 4 (HCV-4) is highly prevalent in Spain, but the information on the molecular characterization of HCV-4 in this region is scarce. Due to this, the molecular characteristics and the evolution of HCV-4 infection in Seville were analyzed (Southern Spain) and compared them with samples from Madrid. HCV genotype was determined by LIPA 2.0 assay and confirmed by sequence analysis of NS5B. Phylogenetic tree was estimated by MEGA 5.10. Bayesian coalescent-based methods were used to estimate the substitution rate and the age of the most recent common ancestor (MRCA). In the phylogenetic analysis of 50 NS5B HCV-4 from Seville and 11 from Madrid, 2 clusters were distinguished: The first cluster (HCV-4a) included 48% of the sequences from Seville and 9% of sequences from Madrid. The second cluster included the remaining sequences belonging to HCV-4d. The mean estimated substitution rate was 2.39 × 10−3 for HCV-4a and 1.81 × 10−3 for HCV-4d for Seville and 2.32 × 10−3 for HCV-4d from Madrid. The date for MRCA was estimated to be around 1981–1984 for HCV-4 from Seville. The dates for MRCA were dated before the recent flow of immigration in Spain. Therefore, the results presented in this study argues against the possibility of a foreign introduction of the HCV-4 from other regions with high prevalence, at least during the last two, decades in which there was a great flow of immigrants. Additionally, an unusual high prevalence of subtype 4a was observed in Seville. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-16 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/1991 Di Lello, Federico Alejandro; Neukam, Karin; Parra Sanchez, Manuel; Plaza, Zulema; Soriano, Vicente; et al.; Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves; Wiley; Journal of Medical Virology; 85; 10; 16-7-2013; 1734-1740 0146-6615 |
url |
http://hdl.handle.net/11336/1991 |
identifier_str_mv |
Di Lello, Federico Alejandro; Neukam, Karin; Parra Sanchez, Manuel; Plaza, Zulema; Soriano, Vicente; et al.; Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves; Wiley; Journal of Medical Virology; 85; 10; 16-7-2013; 1734-1740 0146-6615 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/reference es info:eu-repo/semantics/reference/pmid/23861220 info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jmv.23657/abstract;jsessionid=90A3F350ED36826133985E0FB7BB67F9.f02t01?systemMessage=Wiley+Online+Library+and+related+systems+will+have+3+hours+of+downtime+on+Saturday+12th+September+2015+from+10%3A00-13%3A00+BST+%2F+05%3A00-08%3A00+EDT+%2F+17%3A00-20%3A00+SGT+for+essential+maintenance.++Apologies+for+the+inconvenience. info:eu-repo/semantics/altIdentifier/doi/DOI:10.1002/jmv.23657 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf |
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Wiley |
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Wiley |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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