Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance

Autores
Gázquez, Ayelén; Abdelgawad, Hamada; Baggerman, Geert; Van Raemdonck, Geert; Asard, Han; Maiale, Santiago Javier; Rodriguez, Andres Alberto; Beemster, Gerrit T. S.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We analysed the cellular and molecular changes in the leaf growth zone of tolerant and sensitive rice varieties in response to suboptimal temperatures. Cold reduced the final leaf length by 35% and 51% in tolerant and sensitive varieties, respectively. Tolerant lines exhibited a smaller reduction of the leaf elongation rate and greater compensation by an increased duration of leaf growth. Kinematic analysis showed that cold reduced cell production in the meristem and the expansion rate in the elongation zone, but the latter was compensated for by a doubling of the duration of cell expansion. We performed iTRAQ proteome analysis on proliferating and expanding parts of the leaf growth zone. We identified 559 and 542 proteins, of which 163 and 210 were differentially expressed between zones, and 96 and 68 between treatments, in the tolerant and sensitive lines, respectively. The categories protein biosynthesis and redox homeostasis were significantly overrepresented in the up-regulated proteins. We therefore measured redox metabolites and enzyme activities in the leaf growth zone, demonstrating that tolerance of rice lines to suboptimal temperatures correlates with the ability to up-regulate enzymatic antioxidants in the meristem and non-enzymatic antioxidants in the elongation zone.
Fil: Gázquez, Ayelén. Universiteit Antwerp; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Abdelgawad, Hamada. Universiteit Antwerp; Bélgica
Fil: Baggerman, Geert. Universiteit Antwerp; Bélgica
Fil: Van Raemdonck, Geert. Universiteit Antwerp; Bélgica
Fil: Asard, Han. Universiteit Antwerp; Bélgica
Fil: Maiale, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Rodriguez, Andres Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Beemster, Gerrit T. S.. Universiteit Antwerp; Bélgica
Materia
ITRAQ
KINEMATIC ANALYSIS
LEAF GROWTH
REDOX
RICE
SUBOPTIMAL TEMPERATURE STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/129041
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/129041
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Redox homeostasis in the growth zone of the rice leaf plays a key role in cold toleranceGázquez, AyelénAbdelgawad, HamadaBaggerman, GeertVan Raemdonck, GeertAsard, HanMaiale, Santiago JavierRodriguez, Andres AlbertoBeemster, Gerrit T. S.ITRAQKINEMATIC ANALYSISLEAF GROWTHREDOXRICESUBOPTIMAL TEMPERATURE STRESShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4We analysed the cellular and molecular changes in the leaf growth zone of tolerant and sensitive rice varieties in response to suboptimal temperatures. Cold reduced the final leaf length by 35% and 51% in tolerant and sensitive varieties, respectively. Tolerant lines exhibited a smaller reduction of the leaf elongation rate and greater compensation by an increased duration of leaf growth. Kinematic analysis showed that cold reduced cell production in the meristem and the expansion rate in the elongation zone, but the latter was compensated for by a doubling of the duration of cell expansion. We performed iTRAQ proteome analysis on proliferating and expanding parts of the leaf growth zone. We identified 559 and 542 proteins, of which 163 and 210 were differentially expressed between zones, and 96 and 68 between treatments, in the tolerant and sensitive lines, respectively. The categories protein biosynthesis and redox homeostasis were significantly overrepresented in the up-regulated proteins. We therefore measured redox metabolites and enzyme activities in the leaf growth zone, demonstrating that tolerance of rice lines to suboptimal temperatures correlates with the ability to up-regulate enzymatic antioxidants in the meristem and non-enzymatic antioxidants in the elongation zone.Fil: Gázquez, Ayelén. Universiteit Antwerp; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Abdelgawad, Hamada. Universiteit Antwerp; BélgicaFil: Baggerman, Geert. Universiteit Antwerp; BélgicaFil: Van Raemdonck, Geert. Universiteit Antwerp; BélgicaFil: Asard, Han. Universiteit Antwerp; BélgicaFil: Maiale, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Rodriguez, Andres Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Beemster, Gerrit T. S.. Universiteit Antwerp; BélgicaOxford University Press2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/129041Gázquez, Ayelén; Abdelgawad, Hamada; Baggerman, Geert; Van Raemdonck, Geert; Asard, Han; et al.; Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance; Oxford University Press; Journal of Experimental Botany; 71; 3; 1-2020; 1053-10660022-0957CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/erz455/5590879info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/erz455info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:17:06Zoai:ri.conicet.gov.ar:11336/129041instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:17:06.463CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance
title Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance
spellingShingle Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance
Gázquez, Ayelén
ITRAQ
KINEMATIC ANALYSIS
LEAF GROWTH
REDOX
RICE
SUBOPTIMAL TEMPERATURE STRESS
title_short Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance
title_full Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance
title_fullStr Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance
title_full_unstemmed Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance
title_sort Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance
dc.creator.none.fl_str_mv Gázquez, Ayelén
Abdelgawad, Hamada
Baggerman, Geert
Van Raemdonck, Geert
Asard, Han
Maiale, Santiago Javier
Rodriguez, Andres Alberto
Beemster, Gerrit T. S.
author Gázquez, Ayelén
author_facet Gázquez, Ayelén
Abdelgawad, Hamada
Baggerman, Geert
Van Raemdonck, Geert
Asard, Han
Maiale, Santiago Javier
Rodriguez, Andres Alberto
Beemster, Gerrit T. S.
author_role author
author2 Abdelgawad, Hamada
Baggerman, Geert
Van Raemdonck, Geert
Asard, Han
Maiale, Santiago Javier
Rodriguez, Andres Alberto
Beemster, Gerrit T. S.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ITRAQ
KINEMATIC ANALYSIS
LEAF GROWTH
REDOX
RICE
SUBOPTIMAL TEMPERATURE STRESS
topic ITRAQ
KINEMATIC ANALYSIS
LEAF GROWTH
REDOX
RICE
SUBOPTIMAL TEMPERATURE STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv We analysed the cellular and molecular changes in the leaf growth zone of tolerant and sensitive rice varieties in response to suboptimal temperatures. Cold reduced the final leaf length by 35% and 51% in tolerant and sensitive varieties, respectively. Tolerant lines exhibited a smaller reduction of the leaf elongation rate and greater compensation by an increased duration of leaf growth. Kinematic analysis showed that cold reduced cell production in the meristem and the expansion rate in the elongation zone, but the latter was compensated for by a doubling of the duration of cell expansion. We performed iTRAQ proteome analysis on proliferating and expanding parts of the leaf growth zone. We identified 559 and 542 proteins, of which 163 and 210 were differentially expressed between zones, and 96 and 68 between treatments, in the tolerant and sensitive lines, respectively. The categories protein biosynthesis and redox homeostasis were significantly overrepresented in the up-regulated proteins. We therefore measured redox metabolites and enzyme activities in the leaf growth zone, demonstrating that tolerance of rice lines to suboptimal temperatures correlates with the ability to up-regulate enzymatic antioxidants in the meristem and non-enzymatic antioxidants in the elongation zone.
Fil: Gázquez, Ayelén. Universiteit Antwerp; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Abdelgawad, Hamada. Universiteit Antwerp; Bélgica
Fil: Baggerman, Geert. Universiteit Antwerp; Bélgica
Fil: Van Raemdonck, Geert. Universiteit Antwerp; Bélgica
Fil: Asard, Han. Universiteit Antwerp; Bélgica
Fil: Maiale, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Rodriguez, Andres Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Beemster, Gerrit T. S.. Universiteit Antwerp; Bélgica
description We analysed the cellular and molecular changes in the leaf growth zone of tolerant and sensitive rice varieties in response to suboptimal temperatures. Cold reduced the final leaf length by 35% and 51% in tolerant and sensitive varieties, respectively. Tolerant lines exhibited a smaller reduction of the leaf elongation rate and greater compensation by an increased duration of leaf growth. Kinematic analysis showed that cold reduced cell production in the meristem and the expansion rate in the elongation zone, but the latter was compensated for by a doubling of the duration of cell expansion. We performed iTRAQ proteome analysis on proliferating and expanding parts of the leaf growth zone. We identified 559 and 542 proteins, of which 163 and 210 were differentially expressed between zones, and 96 and 68 between treatments, in the tolerant and sensitive lines, respectively. The categories protein biosynthesis and redox homeostasis were significantly overrepresented in the up-regulated proteins. We therefore measured redox metabolites and enzyme activities in the leaf growth zone, demonstrating that tolerance of rice lines to suboptimal temperatures correlates with the ability to up-regulate enzymatic antioxidants in the meristem and non-enzymatic antioxidants in the elongation zone.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/129041
Gázquez, Ayelén; Abdelgawad, Hamada; Baggerman, Geert; Van Raemdonck, Geert; Asard, Han; et al.; Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance; Oxford University Press; Journal of Experimental Botany; 71; 3; 1-2020; 1053-1066
0022-0957
CONICET Digital
CONICET
url http://hdl.handle.net/11336/129041
identifier_str_mv Gázquez, Ayelén; Abdelgawad, Hamada; Baggerman, Geert; Van Raemdonck, Geert; Asard, Han; et al.; Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance; Oxford University Press; Journal of Experimental Botany; 71; 3; 1-2020; 1053-1066
0022-0957
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/erz455/5590879
info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/erz455
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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