Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea

Autores
Fabro Cerreia Fus, Elena Ines; Almandoz, Gaston Osvaldo; Ferrario, Martha Elba; John, Uwe; Tillmann, Urban; Toebe, Kerstin; Krock, Bernd; Cembella, Allan
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the Argentine Sea, blooms of toxigenic dinoflagellates of the Alexandrium tamarense species complex have led to fish and bird mortalities and human deaths as a consequence of paralytic shellfish poisoning (PSP). Yet little is known about the occurrence of other toxigenic species of the genus Alexandrium, or of their toxin composition beyond coastal waters. The distribution of Alexandrium species and related toxins in the Argentine Sea was determined by sampling surface waters on an oceanographic expedition during austral spring from ~39°S to 48°S. Light microscope and SEM analysis for species identification and enumeration was supplemented by confirmatory PCR analysis from field samples. The most frequent Alexandrium taxon identified by microscopy corresponded to the classical description of A. tamarense. Only weak signals of Group I from the A. tamarense species complex were detected by PCR of bulk field samples, but phylogenetic reconstruction of rDNA sequences from single cells from one station assigned them to ribotype Group I (Alexandrium catenella). PCR probes for Alexandrium minutum and Alexandrium ostenfeldii yielded a positive signal, although A. minutum morphology did not completely match the classical description. Analysis of PSP toxin composition of plankton samples revealed toxin profiles dominated by gonyautoxins (GTX1/4). The main toxic cyclic imine detected was 13‐desMe‐spirolide C and this supported the association with A. ostenfeldii in the field. This study represents the first integrated molecular, morphological and toxinological analysis of field populations of the genus Alexandrium in the Argentine Sea.
Fil: Fabro Cerreia Fus, Elena Ines. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Almandoz, Gaston Osvaldo. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrario, Martha Elba. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: John, Uwe. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; Alemania
Fil: Tillmann, Urban. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; Alemania
Fil: Toebe, Kerstin. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; Alemania
Fil: Krock, Bernd. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; Alemania
Fil: Cembella, Allan. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; Alemania
Materia
Alexandrium Minutum
Alexandrium Ostenfeldii
Lc-Fd
Lc-Ms/Ms
Pcr
Psp
South-Western Atlantic
Spirolides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49791
Acceder
id CONICETDig_4415971cbcb6eeef129d5259d9126062
oai_identifier_str oai:ri.conicet.gov.ar:11336/49791
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine SeaFabro Cerreia Fus, Elena InesAlmandoz, Gaston OsvaldoFerrario, Martha ElbaJohn, UweTillmann, UrbanToebe, KerstinKrock, BerndCembella, AllanAlexandrium MinutumAlexandrium OstenfeldiiLc-FdLc-Ms/MsPcrPspSouth-Western AtlanticSpirolideshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the Argentine Sea, blooms of toxigenic dinoflagellates of the Alexandrium tamarense species complex have led to fish and bird mortalities and human deaths as a consequence of paralytic shellfish poisoning (PSP). Yet little is known about the occurrence of other toxigenic species of the genus Alexandrium, or of their toxin composition beyond coastal waters. The distribution of Alexandrium species and related toxins in the Argentine Sea was determined by sampling surface waters on an oceanographic expedition during austral spring from ~39°S to 48°S. Light microscope and SEM analysis for species identification and enumeration was supplemented by confirmatory PCR analysis from field samples. The most frequent Alexandrium taxon identified by microscopy corresponded to the classical description of A. tamarense. Only weak signals of Group I from the A. tamarense species complex were detected by PCR of bulk field samples, but phylogenetic reconstruction of rDNA sequences from single cells from one station assigned them to ribotype Group I (Alexandrium catenella). PCR probes for Alexandrium minutum and Alexandrium ostenfeldii yielded a positive signal, although A. minutum morphology did not completely match the classical description. Analysis of PSP toxin composition of plankton samples revealed toxin profiles dominated by gonyautoxins (GTX1/4). The main toxic cyclic imine detected was 13‐desMe‐spirolide C and this supported the association with A. ostenfeldii in the field. This study represents the first integrated molecular, morphological and toxinological analysis of field populations of the genus Alexandrium in the Argentine Sea.Fil: Fabro Cerreia Fus, Elena Ines. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Almandoz, Gaston Osvaldo. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrario, Martha Elba. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: John, Uwe. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; AlemaniaFil: Tillmann, Urban. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; AlemaniaFil: Toebe, Kerstin. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; AlemaniaFil: Krock, Bernd. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; AlemaniaFil: Cembella, Allan. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; AlemaniaWiley Blackwell Publishing, Inc2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49791Fabro Cerreia Fus, Elena Ines; Almandoz, Gaston Osvaldo; Ferrario, Martha Elba; John, Uwe; Tillmann, Urban; et al.; Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea; Wiley Blackwell Publishing, Inc; Journal Of Phycology; 53; 6; 12-2017; 1206-12220022-3646CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jpy.12574info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jpy.12574info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:08Zoai:ri.conicet.gov.ar:11336/49791instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:08.52CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
title Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
spellingShingle Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
Fabro Cerreia Fus, Elena Ines
Alexandrium Minutum
Alexandrium Ostenfeldii
Lc-Fd
Lc-Ms/Ms
Pcr
Psp
South-Western Atlantic
Spirolides
title_short Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
title_full Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
title_fullStr Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
title_full_unstemmed Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
title_sort Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
dc.creator.none.fl_str_mv Fabro Cerreia Fus, Elena Ines
Almandoz, Gaston Osvaldo
Ferrario, Martha Elba
John, Uwe
Tillmann, Urban
Toebe, Kerstin
Krock, Bernd
Cembella, Allan
author Fabro Cerreia Fus, Elena Ines
author_facet Fabro Cerreia Fus, Elena Ines
Almandoz, Gaston Osvaldo
Ferrario, Martha Elba
John, Uwe
Tillmann, Urban
Toebe, Kerstin
Krock, Bernd
Cembella, Allan
author_role author
author2 Almandoz, Gaston Osvaldo
Ferrario, Martha Elba
John, Uwe
Tillmann, Urban
Toebe, Kerstin
Krock, Bernd
Cembella, Allan
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Alexandrium Minutum
Alexandrium Ostenfeldii
Lc-Fd
Lc-Ms/Ms
Pcr
Psp
South-Western Atlantic
Spirolides
topic Alexandrium Minutum
Alexandrium Ostenfeldii
Lc-Fd
Lc-Ms/Ms
Pcr
Psp
South-Western Atlantic
Spirolides
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the Argentine Sea, blooms of toxigenic dinoflagellates of the Alexandrium tamarense species complex have led to fish and bird mortalities and human deaths as a consequence of paralytic shellfish poisoning (PSP). Yet little is known about the occurrence of other toxigenic species of the genus Alexandrium, or of their toxin composition beyond coastal waters. The distribution of Alexandrium species and related toxins in the Argentine Sea was determined by sampling surface waters on an oceanographic expedition during austral spring from ~39°S to 48°S. Light microscope and SEM analysis for species identification and enumeration was supplemented by confirmatory PCR analysis from field samples. The most frequent Alexandrium taxon identified by microscopy corresponded to the classical description of A. tamarense. Only weak signals of Group I from the A. tamarense species complex were detected by PCR of bulk field samples, but phylogenetic reconstruction of rDNA sequences from single cells from one station assigned them to ribotype Group I (Alexandrium catenella). PCR probes for Alexandrium minutum and Alexandrium ostenfeldii yielded a positive signal, although A. minutum morphology did not completely match the classical description. Analysis of PSP toxin composition of plankton samples revealed toxin profiles dominated by gonyautoxins (GTX1/4). The main toxic cyclic imine detected was 13‐desMe‐spirolide C and this supported the association with A. ostenfeldii in the field. This study represents the first integrated molecular, morphological and toxinological analysis of field populations of the genus Alexandrium in the Argentine Sea.
Fil: Fabro Cerreia Fus, Elena Ines. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Almandoz, Gaston Osvaldo. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrario, Martha Elba. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: John, Uwe. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; Alemania
Fil: Tillmann, Urban. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; Alemania
Fil: Toebe, Kerstin. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; Alemania
Fil: Krock, Bernd. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; Alemania
Fil: Cembella, Allan. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; Alemania
description In the Argentine Sea, blooms of toxigenic dinoflagellates of the Alexandrium tamarense species complex have led to fish and bird mortalities and human deaths as a consequence of paralytic shellfish poisoning (PSP). Yet little is known about the occurrence of other toxigenic species of the genus Alexandrium, or of their toxin composition beyond coastal waters. The distribution of Alexandrium species and related toxins in the Argentine Sea was determined by sampling surface waters on an oceanographic expedition during austral spring from ~39°S to 48°S. Light microscope and SEM analysis for species identification and enumeration was supplemented by confirmatory PCR analysis from field samples. The most frequent Alexandrium taxon identified by microscopy corresponded to the classical description of A. tamarense. Only weak signals of Group I from the A. tamarense species complex were detected by PCR of bulk field samples, but phylogenetic reconstruction of rDNA sequences from single cells from one station assigned them to ribotype Group I (Alexandrium catenella). PCR probes for Alexandrium minutum and Alexandrium ostenfeldii yielded a positive signal, although A. minutum morphology did not completely match the classical description. Analysis of PSP toxin composition of plankton samples revealed toxin profiles dominated by gonyautoxins (GTX1/4). The main toxic cyclic imine detected was 13‐desMe‐spirolide C and this supported the association with A. ostenfeldii in the field. This study represents the first integrated molecular, morphological and toxinological analysis of field populations of the genus Alexandrium in the Argentine Sea.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49791
Fabro Cerreia Fus, Elena Ines; Almandoz, Gaston Osvaldo; Ferrario, Martha Elba; John, Uwe; Tillmann, Urban; et al.; Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea; Wiley Blackwell Publishing, Inc; Journal Of Phycology; 53; 6; 12-2017; 1206-1222
0022-3646
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49791
identifier_str_mv Fabro Cerreia Fus, Elena Ines; Almandoz, Gaston Osvaldo; Ferrario, Martha Elba; John, Uwe; Tillmann, Urban; et al.; Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea; Wiley Blackwell Publishing, Inc; Journal Of Phycology; 53; 6; 12-2017; 1206-1222
0022-3646
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/jpy.12574
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jpy.12574
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613469206216704
score 13.070432

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