Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1

Autores
Dei Cas, Ignacio; Giliberto, Florencia; Penas Steinhardt, Alberto
Año de publicación
2019
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Psoriasis is a chronic, immune-mediated inflammatory disease. Type 1 psoriasis (Ps1) or early onset psoriasis is the most common form of psoriasis and has a strong genetic component. The gut microbiota is involved in the maturation of the immune system and in multiple metabolic pathways. Disorders in the intestinal bacterial composition or dysbiosis carry important functional consequences and have been implicated in multiple diseases. The objectives of this study were to evaluate differences in the gut microbiota of patients with Ps1 vs. non-psoriatic control subjects (CS). Materials and methods: We studied 38 patients with Ps1 without treatment and 27 CS. Stool samples were collected and the V3-V4 hypervariable regions of the 16S rRNA gene were analyzed using the Illumina MiSeq sequencing platform to determine microbial composition and diversity. Bioinformatic analysis was performed. Results: We did not find differences in biodiversity (alpha diversity) among patients with Ps1 and CS. Beta diversity showed significant differences between both groups (p = 0.032). When comparing the main phyla, only the relative increase of Firmicutes in Ps1 showed significant differences (p < 0.05). At genus level, the LEfSe analysis revealed greater abundance of Faecalibacterium and Blautia in patients with Ps1 and Bacteroides, Paraprevotella, Odoribacter, Anaerotruncus and Oscillospira in CS. Conclusions: The results showed differences in the intestinal microbiota of patients with Ps1 and CS, which would imply that the gut microbiota plays a role in psoriasis pathogenesis.
Fil: Dei Cas, Ignacio. No especifíca;
Fil: Giliberto, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Penas Steinhardt, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina. Universidad Nacional de Lujan. Departamento de Ciencias Básicas. Laboratorio de Genómica Computacional; Argentina
Materia
PSORIASIS
MICROBIOTA INTESTINAL
METAGENOMICA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/118863
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/118863
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1Dei Cas, IgnacioGiliberto, FlorenciaPenas Steinhardt, AlbertoPSORIASISMICROBIOTA INTESTINALMETAGENOMICAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: Psoriasis is a chronic, immune-mediated inflammatory disease. Type 1 psoriasis (Ps1) or early onset psoriasis is the most common form of psoriasis and has a strong genetic component. The gut microbiota is involved in the maturation of the immune system and in multiple metabolic pathways. Disorders in the intestinal bacterial composition or dysbiosis carry important functional consequences and have been implicated in multiple diseases. The objectives of this study were to evaluate differences in the gut microbiota of patients with Ps1 vs. non-psoriatic control subjects (CS). Materials and methods: We studied 38 patients with Ps1 without treatment and 27 CS. Stool samples were collected and the V3-V4 hypervariable regions of the 16S rRNA gene were analyzed using the Illumina MiSeq sequencing platform to determine microbial composition and diversity. Bioinformatic analysis was performed. Results: We did not find differences in biodiversity (alpha diversity) among patients with Ps1 and CS. Beta diversity showed significant differences between both groups (p = 0.032). When comparing the main phyla, only the relative increase of Firmicutes in Ps1 showed significant differences (p < 0.05). At genus level, the LEfSe analysis revealed greater abundance of Faecalibacterium and Blautia in patients with Ps1 and Bacteroides, Paraprevotella, Odoribacter, Anaerotruncus and Oscillospira in CS. Conclusions: The results showed differences in the intestinal microbiota of patients with Ps1 and CS, which would imply that the gut microbiota plays a role in psoriasis pathogenesis.Fil: Dei Cas, Ignacio. No especifíca;Fil: Giliberto, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Penas Steinhardt, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina. Universidad Nacional de Lujan. Departamento de Ciencias Básicas. Laboratorio de Genómica Computacional; ArgentinaSociedad Argentina de Dermatología2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118863Dei Cas, Ignacio; Giliberto, Florencia; Penas Steinhardt, Alberto; Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1; Sociedad Argentina de Dermatología; Dermatología Argentina; 25; 4; 10-2019; 161-1671515-84111669-1636CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://www.dermatolarg.org.ar/index.php/dermatolarg/article/download/1931/1051info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:08Zoai:ri.conicet.gov.ar:11336/118863instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:08.983CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1
title Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1
spellingShingle Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1
Dei Cas, Ignacio
PSORIASIS
MICROBIOTA INTESTINAL
METAGENOMICA
title_short Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1
title_full Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1
title_fullStr Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1
title_full_unstemmed Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1
title_sort Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1
dc.creator.none.fl_str_mv Dei Cas, Ignacio
Giliberto, Florencia
Penas Steinhardt, Alberto
author Dei Cas, Ignacio
author_facet Dei Cas, Ignacio
Giliberto, Florencia
Penas Steinhardt, Alberto
author_role author
author2 Giliberto, Florencia
Penas Steinhardt, Alberto
author2_role author
author
dc.subject.none.fl_str_mv PSORIASIS
MICROBIOTA INTESTINAL
METAGENOMICA
topic PSORIASIS
MICROBIOTA INTESTINAL
METAGENOMICA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction: Psoriasis is a chronic, immune-mediated inflammatory disease. Type 1 psoriasis (Ps1) or early onset psoriasis is the most common form of psoriasis and has a strong genetic component. The gut microbiota is involved in the maturation of the immune system and in multiple metabolic pathways. Disorders in the intestinal bacterial composition or dysbiosis carry important functional consequences and have been implicated in multiple diseases. The objectives of this study were to evaluate differences in the gut microbiota of patients with Ps1 vs. non-psoriatic control subjects (CS). Materials and methods: We studied 38 patients with Ps1 without treatment and 27 CS. Stool samples were collected and the V3-V4 hypervariable regions of the 16S rRNA gene were analyzed using the Illumina MiSeq sequencing platform to determine microbial composition and diversity. Bioinformatic analysis was performed. Results: We did not find differences in biodiversity (alpha diversity) among patients with Ps1 and CS. Beta diversity showed significant differences between both groups (p = 0.032). When comparing the main phyla, only the relative increase of Firmicutes in Ps1 showed significant differences (p < 0.05). At genus level, the LEfSe analysis revealed greater abundance of Faecalibacterium and Blautia in patients with Ps1 and Bacteroides, Paraprevotella, Odoribacter, Anaerotruncus and Oscillospira in CS. Conclusions: The results showed differences in the intestinal microbiota of patients with Ps1 and CS, which would imply that the gut microbiota plays a role in psoriasis pathogenesis.
Fil: Dei Cas, Ignacio. No especifíca;
Fil: Giliberto, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Penas Steinhardt, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina. Universidad Nacional de Lujan. Departamento de Ciencias Básicas. Laboratorio de Genómica Computacional; Argentina
description Introduction: Psoriasis is a chronic, immune-mediated inflammatory disease. Type 1 psoriasis (Ps1) or early onset psoriasis is the most common form of psoriasis and has a strong genetic component. The gut microbiota is involved in the maturation of the immune system and in multiple metabolic pathways. Disorders in the intestinal bacterial composition or dysbiosis carry important functional consequences and have been implicated in multiple diseases. The objectives of this study were to evaluate differences in the gut microbiota of patients with Ps1 vs. non-psoriatic control subjects (CS). Materials and methods: We studied 38 patients with Ps1 without treatment and 27 CS. Stool samples were collected and the V3-V4 hypervariable regions of the 16S rRNA gene were analyzed using the Illumina MiSeq sequencing platform to determine microbial composition and diversity. Bioinformatic analysis was performed. Results: We did not find differences in biodiversity (alpha diversity) among patients with Ps1 and CS. Beta diversity showed significant differences between both groups (p = 0.032). When comparing the main phyla, only the relative increase of Firmicutes in Ps1 showed significant differences (p < 0.05). At genus level, the LEfSe analysis revealed greater abundance of Faecalibacterium and Blautia in patients with Ps1 and Bacteroides, Paraprevotella, Odoribacter, Anaerotruncus and Oscillospira in CS. Conclusions: The results showed differences in the intestinal microbiota of patients with Ps1 and CS, which would imply that the gut microbiota plays a role in psoriasis pathogenesis.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118863
Dei Cas, Ignacio; Giliberto, Florencia; Penas Steinhardt, Alberto; Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1; Sociedad Argentina de Dermatología; Dermatología Argentina; 25; 4; 10-2019; 161-167
1515-8411
1669-1636
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118863
identifier_str_mv Dei Cas, Ignacio; Giliberto, Florencia; Penas Steinhardt, Alberto; Análisis metagenómico de la microbiota intestinal en pacientes con psoriasis de tipo 1; Sociedad Argentina de Dermatología; Dermatología Argentina; 25; 4; 10-2019; 161-167
1515-8411
1669-1636
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.dermatolarg.org.ar/index.php/dermatolarg/article/download/1931/1051
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Argentina de Dermatología
publisher.none.fl_str_mv Sociedad Argentina de Dermatología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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