Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis

Autores
Stern, David L.; Crocker, Justin; Ding, Yun; Frankel, Nicolás; Kappes, Gretchen; Kim, Elizabeth; Kuzmickas, Ryan; Lemire, Andrew; Mast, Joshua D.; Picard, Serge
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagents. Here, we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mapped piggyBac transposons including an enhanced yellow fluorescent protein (EYFP) gene expressed in the eyes and a φC31 attP site-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 58 transposable elements in D. virilis that will be useful for genetic mapping studies.
Fil: Stern, David L.. Janelia Research Campus; Estados Unidos
Fil: Crocker, Justin. Janelia Research Campus; Estados Unidos
Fil: Ding, Yun. Janelia Research Campus; Estados Unidos
Fil: Frankel, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Kappes, Gretchen. No especifica;
Fil: Kim, Elizabeth. Janelia Research Campus; Estados Unidos
Fil: Kuzmickas, Ryan. Brigham and Women's Hospital; Estados Unidos
Fil: Lemire, Andrew. Janelia Research Campus; Estados Unidos
Fil: Mast, Joshua D.. California State University; Estados Unidos
Fil: Picard, Serge. University of Princeton; Estados Unidos
Materia
DROSOPHILA
EVOLUTION
GENETICS
SPECIATION
TRANSGENICS
ΦC31 INTEGRASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64980
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/64980
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilisStern, David L.Crocker, JustinDing, YunFrankel, NicolásKappes, GretchenKim, ElizabethKuzmickas, RyanLemire, AndrewMast, Joshua D.Picard, SergeDROSOPHILAEVOLUTIONGENETICSSPECIATIONTRANSGENICSΦC31 INTEGRASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagents. Here, we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mapped piggyBac transposons including an enhanced yellow fluorescent protein (EYFP) gene expressed in the eyes and a φC31 attP site-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 58 transposable elements in D. virilis that will be useful for genetic mapping studies.Fil: Stern, David L.. Janelia Research Campus; Estados UnidosFil: Crocker, Justin. Janelia Research Campus; Estados UnidosFil: Ding, Yun. Janelia Research Campus; Estados UnidosFil: Frankel, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Kappes, Gretchen. No especifica;Fil: Kim, Elizabeth. Janelia Research Campus; Estados UnidosFil: Kuzmickas, Ryan. Brigham and Women's Hospital; Estados UnidosFil: Lemire, Andrew. Janelia Research Campus; Estados UnidosFil: Mast, Joshua D.. California State University; Estados UnidosFil: Picard, Serge. University of Princeton; Estados UnidosGenetics Society of America2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64980Stern, David L.; Crocker, Justin; Ding, Yun; Frankel, Nicolás; Kappes, Gretchen; et al.; Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis; Genetics Society of America; G3: Genes, Genomes, Genetics; 7; 4; 4-2017; 1339-13472160-1836CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1534/g3.116.038885info:eu-repo/semantics/altIdentifier/url/http://www.g3journal.org/content/7/4/1339info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:31Zoai:ri.conicet.gov.ar:11336/64980instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:31.876CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis
title Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis
spellingShingle Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis
Stern, David L.
DROSOPHILA
EVOLUTION
GENETICS
SPECIATION
TRANSGENICS
ΦC31 INTEGRASE
title_short Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis
title_full Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis
title_fullStr Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis
title_full_unstemmed Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis
title_sort Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis
dc.creator.none.fl_str_mv Stern, David L.
Crocker, Justin
Ding, Yun
Frankel, Nicolás
Kappes, Gretchen
Kim, Elizabeth
Kuzmickas, Ryan
Lemire, Andrew
Mast, Joshua D.
Picard, Serge
author Stern, David L.
author_facet Stern, David L.
Crocker, Justin
Ding, Yun
Frankel, Nicolás
Kappes, Gretchen
Kim, Elizabeth
Kuzmickas, Ryan
Lemire, Andrew
Mast, Joshua D.
Picard, Serge
author_role author
author2 Crocker, Justin
Ding, Yun
Frankel, Nicolás
Kappes, Gretchen
Kim, Elizabeth
Kuzmickas, Ryan
Lemire, Andrew
Mast, Joshua D.
Picard, Serge
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DROSOPHILA
EVOLUTION
GENETICS
SPECIATION
TRANSGENICS
ΦC31 INTEGRASE
topic DROSOPHILA
EVOLUTION
GENETICS
SPECIATION
TRANSGENICS
ΦC31 INTEGRASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagents. Here, we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mapped piggyBac transposons including an enhanced yellow fluorescent protein (EYFP) gene expressed in the eyes and a φC31 attP site-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 58 transposable elements in D. virilis that will be useful for genetic mapping studies.
Fil: Stern, David L.. Janelia Research Campus; Estados Unidos
Fil: Crocker, Justin. Janelia Research Campus; Estados Unidos
Fil: Ding, Yun. Janelia Research Campus; Estados Unidos
Fil: Frankel, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Kappes, Gretchen. No especifica;
Fil: Kim, Elizabeth. Janelia Research Campus; Estados Unidos
Fil: Kuzmickas, Ryan. Brigham and Women's Hospital; Estados Unidos
Fil: Lemire, Andrew. Janelia Research Campus; Estados Unidos
Fil: Mast, Joshua D.. California State University; Estados Unidos
Fil: Picard, Serge. University of Princeton; Estados Unidos
description Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagents. Here, we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mapped piggyBac transposons including an enhanced yellow fluorescent protein (EYFP) gene expressed in the eyes and a φC31 attP site-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 58 transposable elements in D. virilis that will be useful for genetic mapping studies.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64980
Stern, David L.; Crocker, Justin; Ding, Yun; Frankel, Nicolás; Kappes, Gretchen; et al.; Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis; Genetics Society of America; G3: Genes, Genomes, Genetics; 7; 4; 4-2017; 1339-1347
2160-1836
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64980
identifier_str_mv Stern, David L.; Crocker, Justin; Ding, Yun; Frankel, Nicolás; Kappes, Gretchen; et al.; Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis; Genetics Society of America; G3: Genes, Genomes, Genetics; 7; 4; 4-2017; 1339-1347
2160-1836
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1534/g3.116.038885
info:eu-repo/semantics/altIdentifier/url/http://www.g3journal.org/content/7/4/1339
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Genetics Society of America
publisher.none.fl_str_mv Genetics Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613655185850368
score 13.260194

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