DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation

Autores
Oliferuk, Sonia; Ródenas, Reyes; Pérez, Adriana Alicia; Martínez, Vicente; Rubio, Francisco; Santa Maria, Guillermo Esteban
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
DELLAs proteins play a major role in the modulation of plant responses to fluctuations in environmental conditions. In this work, we examined to what extent Arabidopsis thaliana plants lacking DELLAs activity (5xdella mutant) or carrying an altered function allele of one of the DELLAs coding genes (gai-1 mutant) display differential responses, in terms of growth and shoot elemental composition, relative to WT plants when deprived of potassium (K). Studies with plants grown in hydroponic media unveiled that the shoot mineral composition of gai-1 constitutively differs from that of WT and 5xdella plants. Tolerance to K-deprivation, as estimated by the relative decline of biomass accumulation, followed the order gai-1 > WT > 5xdella. In turn, the degree of responsiveness of the shoot composition to the stress condition followed the order 5xdella > WT > gai-1, suggesting a correspondence between the degree of injury and changes in the elemental composition. Internal efficiency of K-utilization was maximized in WT relative to 5xdella plants. Interestingly, the acquisition of K was severely impaired in gai-1 plants well supplied, or deprived of, K. Complementary studies indicated that influx and root-to-shoot transport of Rubidium, a K-analogue, were reduced in those plants. Furthermore, evidence obtained supports the view that the effect of altered DELLAs derives, at least partially, from controlling the accumulation of transcripts coding for the AtHAK5 transporter. These results, together with the observation that K-deprivation promotes the accumulation of a DELLA protein (RGA) fused to GFP in root cells, suggest a pivotal role of DELLAs in key plant responses to K-deprivation.
Fil: Oliferuk, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Ródenas, Reyes. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Pérez, Adriana Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Martínez, Vicente. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Rubio, Francisco. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Santa Maria, Guillermo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Materia
Arabidopsis Thaliana
Della
Ionome
Potassium
Uptake
Utilization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55180
Acceder
id CONICETDig_425ebdfc33ca43f784724ddac0d62afb
oai_identifier_str oai:ri.conicet.gov.ar:11336/55180
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium DeprivationOliferuk, SoniaRódenas, ReyesPérez, Adriana AliciaMartínez, VicenteRubio, FranciscoSanta Maria, Guillermo EstebanArabidopsis ThalianaDellaIonomePotassiumUptakeUtilizationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1DELLAs proteins play a major role in the modulation of plant responses to fluctuations in environmental conditions. In this work, we examined to what extent Arabidopsis thaliana plants lacking DELLAs activity (5xdella mutant) or carrying an altered function allele of one of the DELLAs coding genes (gai-1 mutant) display differential responses, in terms of growth and shoot elemental composition, relative to WT plants when deprived of potassium (K). Studies with plants grown in hydroponic media unveiled that the shoot mineral composition of gai-1 constitutively differs from that of WT and 5xdella plants. Tolerance to K-deprivation, as estimated by the relative decline of biomass accumulation, followed the order gai-1 > WT > 5xdella. In turn, the degree of responsiveness of the shoot composition to the stress condition followed the order 5xdella > WT > gai-1, suggesting a correspondence between the degree of injury and changes in the elemental composition. Internal efficiency of K-utilization was maximized in WT relative to 5xdella plants. Interestingly, the acquisition of K was severely impaired in gai-1 plants well supplied, or deprived of, K. Complementary studies indicated that influx and root-to-shoot transport of Rubidium, a K-analogue, were reduced in those plants. Furthermore, evidence obtained supports the view that the effect of altered DELLAs derives, at least partially, from controlling the accumulation of transcripts coding for the AtHAK5 transporter. These results, together with the observation that K-deprivation promotes the accumulation of a DELLA protein (RGA) fused to GFP in root cells, suggest a pivotal role of DELLAs in key plant responses to K-deprivation.Fil: Oliferuk, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Ródenas, Reyes. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; EspañaFil: Pérez, Adriana Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Martínez, Vicente. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; EspañaFil: Rubio, Francisco. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; EspañaFil: Santa Maria, Guillermo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaSpringer2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55180Oliferuk, Sonia; Ródenas, Reyes; Pérez, Adriana Alicia; Martínez, Vicente; Rubio, Francisco; et al.; DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation; Springer; Journal of Plant Growth Regulation; 36; 2; 6-2017; 487-5010721-75951435-8107CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00344-016-9656-4info:eu-repo/semantics/altIdentifier/doi/10.1007/s00344-016-9656-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:46Zoai:ri.conicet.gov.ar:11336/55180instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:46.97CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation
title DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation
spellingShingle DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation
Oliferuk, Sonia
Arabidopsis Thaliana
Della
Ionome
Potassium
Uptake
Utilization
title_short DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation
title_full DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation
title_fullStr DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation
title_full_unstemmed DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation
title_sort DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation
dc.creator.none.fl_str_mv Oliferuk, Sonia
Ródenas, Reyes
Pérez, Adriana Alicia
Martínez, Vicente
Rubio, Francisco
Santa Maria, Guillermo Esteban
author Oliferuk, Sonia
author_facet Oliferuk, Sonia
Ródenas, Reyes
Pérez, Adriana Alicia
Martínez, Vicente
Rubio, Francisco
Santa Maria, Guillermo Esteban
author_role author
author2 Ródenas, Reyes
Pérez, Adriana Alicia
Martínez, Vicente
Rubio, Francisco
Santa Maria, Guillermo Esteban
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis Thaliana
Della
Ionome
Potassium
Uptake
Utilization
topic Arabidopsis Thaliana
Della
Ionome
Potassium
Uptake
Utilization
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv DELLAs proteins play a major role in the modulation of plant responses to fluctuations in environmental conditions. In this work, we examined to what extent Arabidopsis thaliana plants lacking DELLAs activity (5xdella mutant) or carrying an altered function allele of one of the DELLAs coding genes (gai-1 mutant) display differential responses, in terms of growth and shoot elemental composition, relative to WT plants when deprived of potassium (K). Studies with plants grown in hydroponic media unveiled that the shoot mineral composition of gai-1 constitutively differs from that of WT and 5xdella plants. Tolerance to K-deprivation, as estimated by the relative decline of biomass accumulation, followed the order gai-1 > WT > 5xdella. In turn, the degree of responsiveness of the shoot composition to the stress condition followed the order 5xdella > WT > gai-1, suggesting a correspondence between the degree of injury and changes in the elemental composition. Internal efficiency of K-utilization was maximized in WT relative to 5xdella plants. Interestingly, the acquisition of K was severely impaired in gai-1 plants well supplied, or deprived of, K. Complementary studies indicated that influx and root-to-shoot transport of Rubidium, a K-analogue, were reduced in those plants. Furthermore, evidence obtained supports the view that the effect of altered DELLAs derives, at least partially, from controlling the accumulation of transcripts coding for the AtHAK5 transporter. These results, together with the observation that K-deprivation promotes the accumulation of a DELLA protein (RGA) fused to GFP in root cells, suggest a pivotal role of DELLAs in key plant responses to K-deprivation.
Fil: Oliferuk, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Ródenas, Reyes. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Pérez, Adriana Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Martínez, Vicente. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Rubio, Francisco. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Santa Maria, Guillermo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
description DELLAs proteins play a major role in the modulation of plant responses to fluctuations in environmental conditions. In this work, we examined to what extent Arabidopsis thaliana plants lacking DELLAs activity (5xdella mutant) or carrying an altered function allele of one of the DELLAs coding genes (gai-1 mutant) display differential responses, in terms of growth and shoot elemental composition, relative to WT plants when deprived of potassium (K). Studies with plants grown in hydroponic media unveiled that the shoot mineral composition of gai-1 constitutively differs from that of WT and 5xdella plants. Tolerance to K-deprivation, as estimated by the relative decline of biomass accumulation, followed the order gai-1 > WT > 5xdella. In turn, the degree of responsiveness of the shoot composition to the stress condition followed the order 5xdella > WT > gai-1, suggesting a correspondence between the degree of injury and changes in the elemental composition. Internal efficiency of K-utilization was maximized in WT relative to 5xdella plants. Interestingly, the acquisition of K was severely impaired in gai-1 plants well supplied, or deprived of, K. Complementary studies indicated that influx and root-to-shoot transport of Rubidium, a K-analogue, were reduced in those plants. Furthermore, evidence obtained supports the view that the effect of altered DELLAs derives, at least partially, from controlling the accumulation of transcripts coding for the AtHAK5 transporter. These results, together with the observation that K-deprivation promotes the accumulation of a DELLA protein (RGA) fused to GFP in root cells, suggest a pivotal role of DELLAs in key plant responses to K-deprivation.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55180
Oliferuk, Sonia; Ródenas, Reyes; Pérez, Adriana Alicia; Martínez, Vicente; Rubio, Francisco; et al.; DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation; Springer; Journal of Plant Growth Regulation; 36; 2; 6-2017; 487-501
0721-7595
1435-8107
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55180
identifier_str_mv Oliferuk, Sonia; Ródenas, Reyes; Pérez, Adriana Alicia; Martínez, Vicente; Rubio, Francisco; et al.; DELLAs Contribute to Set the Growth and Mineral Composition of Arabidopsis thaliana Plants Grown Under Conditions of Potassium Deprivation; Springer; Journal of Plant Growth Regulation; 36; 2; 6-2017; 487-501
0721-7595
1435-8107
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00344-016-9656-4
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00344-016-9656-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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