Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study

Autores
Diamanti, Eleftheria; Gutiérrez Pineda, Eduart Andrés; Politakos, Nikolaos; Andreozzi, Patrizia; Rodriguez Presa, Maria Jose; Knoll, Wolfgang; Azzaroni, Omar; Gervasi, Claudio; Moya, Sergio E.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Supported membranes on polymer cushions are of fundamental interest as models for cell membranes. The use of polyelectrolyte multilayers (PEMs) assembled by the layer by layer (LbL) technique as supports for a bilayer allows for easy integration of the lipid bilayer on surfaces and devices and for nanoscale tunable spacing of the lipid bilayer. Controlling ionic permeability in lipid bilayers supported on PEMs triggers potential applications in sensing and as models for transport phenomena in cell membranes. Lipid bilayers displaying gramicidin channels are fabricated on top of polyallylamine hydrochloride (PAH) and polystyrene sulfonate (PSS) multilayer films, by the assembly of vesicles of phosphatidylcholine and phosphatidylserine, 50:50 M/M, carrying gramicidin (GA). Quartz crystal microbalance with dissipation shows that the vesicles with GA fuse into a bilayer. Atomic force microscopy reveals that the presence of GA alters the bilayer topography resulting in depressions in the bilayer of around 70 nm in diameter. Electrochemical impedance spectroscopy (EIS) studies show that supported bilayers carrying GA have smaller resistances than the bilayers without GA. Lipid layers carrying GA display a higher conductance for K+ than for Na+ and are blocked in the presence of Ca2+.
Fil: Diamanti, Eleftheria. CIC biomaGUNE; España
Fil: Gutiérrez Pineda, Eduart Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ingeniería; Argentina
Fil: Politakos, Nikolaos. CIC biomaGUNE; España
Fil: Andreozzi, Patrizia. CIC biomaGUNE; España
Fil: Rodriguez Presa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Knoll, Wolfgang. Austrian Institute of Technology; Austria
Fil: Azzaroni, Omar. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Gervasi, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ingeniería; Argentina
Fil: Moya, Sergio E.. CIC biomaGUNE; España
Materia
No posee
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63965
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/63965
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance studyDiamanti, EleftheriaGutiérrez Pineda, Eduart AndrésPolitakos, NikolaosAndreozzi, PatriziaRodriguez Presa, Maria JoseKnoll, WolfgangAzzaroni, OmarGervasi, ClaudioMoya, Sergio E.No poseehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Supported membranes on polymer cushions are of fundamental interest as models for cell membranes. The use of polyelectrolyte multilayers (PEMs) assembled by the layer by layer (LbL) technique as supports for a bilayer allows for easy integration of the lipid bilayer on surfaces and devices and for nanoscale tunable spacing of the lipid bilayer. Controlling ionic permeability in lipid bilayers supported on PEMs triggers potential applications in sensing and as models for transport phenomena in cell membranes. Lipid bilayers displaying gramicidin channels are fabricated on top of polyallylamine hydrochloride (PAH) and polystyrene sulfonate (PSS) multilayer films, by the assembly of vesicles of phosphatidylcholine and phosphatidylserine, 50:50 M/M, carrying gramicidin (GA). Quartz crystal microbalance with dissipation shows that the vesicles with GA fuse into a bilayer. Atomic force microscopy reveals that the presence of GA alters the bilayer topography resulting in depressions in the bilayer of around 70 nm in diameter. Electrochemical impedance spectroscopy (EIS) studies show that supported bilayers carrying GA have smaller resistances than the bilayers without GA. Lipid layers carrying GA display a higher conductance for K+ than for Na+ and are blocked in the presence of Ca2+.Fil: Diamanti, Eleftheria. CIC biomaGUNE; EspañaFil: Gutiérrez Pineda, Eduart Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ingeniería; ArgentinaFil: Politakos, Nikolaos. CIC biomaGUNE; EspañaFil: Andreozzi, Patrizia. CIC biomaGUNE; EspañaFil: Rodriguez Presa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Knoll, Wolfgang. Austrian Institute of Technology; AustriaFil: Azzaroni, Omar. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Gervasi, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ingeniería; ArgentinaFil: Moya, Sergio E.. CIC biomaGUNE; EspañaRoyal Society of Chemistry2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63965Diamanti, Eleftheria; Gutiérrez Pineda, Eduart Andrés; Politakos, Nikolaos; Andreozzi, Patrizia; Rodriguez Presa, Maria Jose; et al.; Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study; Royal Society of Chemistry; Soft Matter; 13; 47; 11-2017; 8922-89291744-683XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/c7sm01539ainfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/Content/ArticleLanding/2017/SM/C7SM01539Ainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:00Zoai:ri.conicet.gov.ar:11336/63965instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:01.212CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study
title Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study
spellingShingle Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study
Diamanti, Eleftheria
No posee
title_short Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study
title_full Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study
title_fullStr Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study
title_full_unstemmed Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study
title_sort Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study
dc.creator.none.fl_str_mv Diamanti, Eleftheria
Gutiérrez Pineda, Eduart Andrés
Politakos, Nikolaos
Andreozzi, Patrizia
Rodriguez Presa, Maria Jose
Knoll, Wolfgang
Azzaroni, Omar
Gervasi, Claudio
Moya, Sergio E.
author Diamanti, Eleftheria
author_facet Diamanti, Eleftheria
Gutiérrez Pineda, Eduart Andrés
Politakos, Nikolaos
Andreozzi, Patrizia
Rodriguez Presa, Maria Jose
Knoll, Wolfgang
Azzaroni, Omar
Gervasi, Claudio
Moya, Sergio E.
author_role author
author2 Gutiérrez Pineda, Eduart Andrés
Politakos, Nikolaos
Andreozzi, Patrizia
Rodriguez Presa, Maria Jose
Knoll, Wolfgang
Azzaroni, Omar
Gervasi, Claudio
Moya, Sergio E.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv No posee
topic No posee
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Supported membranes on polymer cushions are of fundamental interest as models for cell membranes. The use of polyelectrolyte multilayers (PEMs) assembled by the layer by layer (LbL) technique as supports for a bilayer allows for easy integration of the lipid bilayer on surfaces and devices and for nanoscale tunable spacing of the lipid bilayer. Controlling ionic permeability in lipid bilayers supported on PEMs triggers potential applications in sensing and as models for transport phenomena in cell membranes. Lipid bilayers displaying gramicidin channels are fabricated on top of polyallylamine hydrochloride (PAH) and polystyrene sulfonate (PSS) multilayer films, by the assembly of vesicles of phosphatidylcholine and phosphatidylserine, 50:50 M/M, carrying gramicidin (GA). Quartz crystal microbalance with dissipation shows that the vesicles with GA fuse into a bilayer. Atomic force microscopy reveals that the presence of GA alters the bilayer topography resulting in depressions in the bilayer of around 70 nm in diameter. Electrochemical impedance spectroscopy (EIS) studies show that supported bilayers carrying GA have smaller resistances than the bilayers without GA. Lipid layers carrying GA display a higher conductance for K+ than for Na+ and are blocked in the presence of Ca2+.
Fil: Diamanti, Eleftheria. CIC biomaGUNE; España
Fil: Gutiérrez Pineda, Eduart Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ingeniería; Argentina
Fil: Politakos, Nikolaos. CIC biomaGUNE; España
Fil: Andreozzi, Patrizia. CIC biomaGUNE; España
Fil: Rodriguez Presa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Knoll, Wolfgang. Austrian Institute of Technology; Austria
Fil: Azzaroni, Omar. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Gervasi, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ingeniería; Argentina
Fil: Moya, Sergio E.. CIC biomaGUNE; España
description Supported membranes on polymer cushions are of fundamental interest as models for cell membranes. The use of polyelectrolyte multilayers (PEMs) assembled by the layer by layer (LbL) technique as supports for a bilayer allows for easy integration of the lipid bilayer on surfaces and devices and for nanoscale tunable spacing of the lipid bilayer. Controlling ionic permeability in lipid bilayers supported on PEMs triggers potential applications in sensing and as models for transport phenomena in cell membranes. Lipid bilayers displaying gramicidin channels are fabricated on top of polyallylamine hydrochloride (PAH) and polystyrene sulfonate (PSS) multilayer films, by the assembly of vesicles of phosphatidylcholine and phosphatidylserine, 50:50 M/M, carrying gramicidin (GA). Quartz crystal microbalance with dissipation shows that the vesicles with GA fuse into a bilayer. Atomic force microscopy reveals that the presence of GA alters the bilayer topography resulting in depressions in the bilayer of around 70 nm in diameter. Electrochemical impedance spectroscopy (EIS) studies show that supported bilayers carrying GA have smaller resistances than the bilayers without GA. Lipid layers carrying GA display a higher conductance for K+ than for Na+ and are blocked in the presence of Ca2+.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63965
Diamanti, Eleftheria; Gutiérrez Pineda, Eduart Andrés; Politakos, Nikolaos; Andreozzi, Patrizia; Rodriguez Presa, Maria Jose; et al.; Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study; Royal Society of Chemistry; Soft Matter; 13; 47; 11-2017; 8922-8929
1744-683X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63965
identifier_str_mv Diamanti, Eleftheria; Gutiérrez Pineda, Eduart Andrés; Politakos, Nikolaos; Andreozzi, Patrizia; Rodriguez Presa, Maria Jose; et al.; Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: An electrochemical impedance study; Royal Society of Chemistry; Soft Matter; 13; 47; 11-2017; 8922-8929
1744-683X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/c7sm01539a
info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/Content/ArticleLanding/2017/SM/C7SM01539A
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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