Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves

Autores
Liron, Juan Pedro; Fernandez, María Elena; Prando, Alberto José; Baldo, Andres; Giovambattista, Guillermo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In prepubertal bull calves there is an early transient rise in gonadotropin secretion between 10 and 20 weeks of age. The early elevation in mean circulating concentrations of LH and FSH most likely causes the proliferation of Sertoli and Leydig cells, respectively. This postnatal gonadotropin rise it is considered one of the main factors in determining the age at which bulls reach puberty (Brito et al., 2007). In order to contribute our understanding of the genes and regulatory pathways involved in this phenomenon, we characterized the hypothalamus transcriptome from six Angus calves along this early expression pattern of LH (4, 6 and 14 wk of age). Of 37 million RNA-Seq reads per sample generated using the Illumina HiSeq 2000 sequencer, at least 95% were mapped to the customized reference genome BosTau6. The Gene annotation revealed that 13976 genes were expressed in the hypothalamus libraries. Tophat2, EdgeR, DESeq2, Bioconductor and R packages were utilized to performed differential expression analysis between groups. We detected 915 differentially expressed genes (P adjusted < 0.05). The top gene ontology term enrichment of the highest expressed genes in the hypothalamus included cellular synapse, ion chanel complex, neuron projection and plasma membrane part (Cellular component category); cell-cell signaling, transmembrane transport, behavior and organism process (Biological process); metal ion transmembrane transporter activity and neuropeptide hormone activity (Molecular function). Enrichment analysis identified 40 KEGG significantly enriched pathways. Based on the observation of the lowest P-value, calcium, oxytocin, circadian entrainment, cholinergic glutamatergic, dopaminergic, serotonergic and GABAergic synapse, GnRH, Estrogen, Rap1, MAPK, ErbB, Ras and cancer signaling and several drugs addiction were among the most important enriched pathways. At the top list the DGs we found a group of genes including the homeodomain transcription factor Orthopedia OTP and the coregulated genes AVP, OXT, CRH and TH known for their physiological roles associated with lactation and mammalian social behaviors.
Fil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Fernandez, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Prando, Alberto José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Baldo, Andres. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
36th International Society for Animal Genetics Conference
Dublin
Irlanda
International Society for Animal Genetics
Materia
Cattle
Functional genomics
RNA-seq
Puberty
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/174863
Acceder
id CONICETDig_4218008c84af698e5d0e9f57730fa88e
oai_identifier_str oai:ri.conicet.gov.ar:11336/174863
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calvesLiron, Juan PedroFernandez, María ElenaPrando, Alberto JoséBaldo, AndresGiovambattista, GuillermoCattleFunctional genomicsRNA-seqPubertyhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4In prepubertal bull calves there is an early transient rise in gonadotropin secretion between 10 and 20 weeks of age. The early elevation in mean circulating concentrations of LH and FSH most likely causes the proliferation of Sertoli and Leydig cells, respectively. This postnatal gonadotropin rise it is considered one of the main factors in determining the age at which bulls reach puberty (Brito et al., 2007). In order to contribute our understanding of the genes and regulatory pathways involved in this phenomenon, we characterized the hypothalamus transcriptome from six Angus calves along this early expression pattern of LH (4, 6 and 14 wk of age). Of 37 million RNA-Seq reads per sample generated using the Illumina HiSeq 2000 sequencer, at least 95% were mapped to the customized reference genome BosTau6. The Gene annotation revealed that 13976 genes were expressed in the hypothalamus libraries. Tophat2, EdgeR, DESeq2, Bioconductor and R packages were utilized to performed differential expression analysis between groups. We detected 915 differentially expressed genes (P adjusted < 0.05). The top gene ontology term enrichment of the highest expressed genes in the hypothalamus included cellular synapse, ion chanel complex, neuron projection and plasma membrane part (Cellular component category); cell-cell signaling, transmembrane transport, behavior and organism process (Biological process); metal ion transmembrane transporter activity and neuropeptide hormone activity (Molecular function). Enrichment analysis identified 40 KEGG significantly enriched pathways. Based on the observation of the lowest P-value, calcium, oxytocin, circadian entrainment, cholinergic glutamatergic, dopaminergic, serotonergic and GABAergic synapse, GnRH, Estrogen, Rap1, MAPK, ErbB, Ras and cancer signaling and several drugs addiction were among the most important enriched pathways. At the top list the DGs we found a group of genes including the homeodomain transcription factor Orthopedia OTP and the coregulated genes AVP, OXT, CRH and TH known for their physiological roles associated with lactation and mammalian social behaviors.Fil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Fernandez, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Prando, Alberto José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Baldo, Andres. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina36th International Society for Animal Genetics ConferenceDublinIrlandaInternational Society for Animal GeneticsInternational Society for Animal Genetics2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/174863Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves; 36th International Society for Animal Genetics Conference; Dublin; Irlanda; 2017; 48-49CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.isag.us/2017/info:eu-repo/semantics/altIdentifier/url/https://www.isag.us/Docs/Proceedings/ISAG2017_Proceedings.pdf?v3Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:31:41Zoai:ri.conicet.gov.ar:11336/174863instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:31:42.204CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves
title Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves
spellingShingle Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves
Liron, Juan Pedro
Cattle
Functional genomics
RNA-seq
Puberty
title_short Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves
title_full Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves
title_fullStr Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves
title_full_unstemmed Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves
title_sort Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves
dc.creator.none.fl_str_mv Liron, Juan Pedro
Fernandez, María Elena
Prando, Alberto José
Baldo, Andres
Giovambattista, Guillermo
author Liron, Juan Pedro
author_facet Liron, Juan Pedro
Fernandez, María Elena
Prando, Alberto José
Baldo, Andres
Giovambattista, Guillermo
author_role author
author2 Fernandez, María Elena
Prando, Alberto José
Baldo, Andres
Giovambattista, Guillermo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cattle
Functional genomics
RNA-seq
Puberty
topic Cattle
Functional genomics
RNA-seq
Puberty
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In prepubertal bull calves there is an early transient rise in gonadotropin secretion between 10 and 20 weeks of age. The early elevation in mean circulating concentrations of LH and FSH most likely causes the proliferation of Sertoli and Leydig cells, respectively. This postnatal gonadotropin rise it is considered one of the main factors in determining the age at which bulls reach puberty (Brito et al., 2007). In order to contribute our understanding of the genes and regulatory pathways involved in this phenomenon, we characterized the hypothalamus transcriptome from six Angus calves along this early expression pattern of LH (4, 6 and 14 wk of age). Of 37 million RNA-Seq reads per sample generated using the Illumina HiSeq 2000 sequencer, at least 95% were mapped to the customized reference genome BosTau6. The Gene annotation revealed that 13976 genes were expressed in the hypothalamus libraries. Tophat2, EdgeR, DESeq2, Bioconductor and R packages were utilized to performed differential expression analysis between groups. We detected 915 differentially expressed genes (P adjusted < 0.05). The top gene ontology term enrichment of the highest expressed genes in the hypothalamus included cellular synapse, ion chanel complex, neuron projection and plasma membrane part (Cellular component category); cell-cell signaling, transmembrane transport, behavior and organism process (Biological process); metal ion transmembrane transporter activity and neuropeptide hormone activity (Molecular function). Enrichment analysis identified 40 KEGG significantly enriched pathways. Based on the observation of the lowest P-value, calcium, oxytocin, circadian entrainment, cholinergic glutamatergic, dopaminergic, serotonergic and GABAergic synapse, GnRH, Estrogen, Rap1, MAPK, ErbB, Ras and cancer signaling and several drugs addiction were among the most important enriched pathways. At the top list the DGs we found a group of genes including the homeodomain transcription factor Orthopedia OTP and the coregulated genes AVP, OXT, CRH and TH known for their physiological roles associated with lactation and mammalian social behaviors.
Fil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Fernandez, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Prando, Alberto José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Baldo, Andres. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
36th International Society for Animal Genetics Conference
Dublin
Irlanda
International Society for Animal Genetics
description In prepubertal bull calves there is an early transient rise in gonadotropin secretion between 10 and 20 weeks of age. The early elevation in mean circulating concentrations of LH and FSH most likely causes the proliferation of Sertoli and Leydig cells, respectively. This postnatal gonadotropin rise it is considered one of the main factors in determining the age at which bulls reach puberty (Brito et al., 2007). In order to contribute our understanding of the genes and regulatory pathways involved in this phenomenon, we characterized the hypothalamus transcriptome from six Angus calves along this early expression pattern of LH (4, 6 and 14 wk of age). Of 37 million RNA-Seq reads per sample generated using the Illumina HiSeq 2000 sequencer, at least 95% were mapped to the customized reference genome BosTau6. The Gene annotation revealed that 13976 genes were expressed in the hypothalamus libraries. Tophat2, EdgeR, DESeq2, Bioconductor and R packages were utilized to performed differential expression analysis between groups. We detected 915 differentially expressed genes (P adjusted < 0.05). The top gene ontology term enrichment of the highest expressed genes in the hypothalamus included cellular synapse, ion chanel complex, neuron projection and plasma membrane part (Cellular component category); cell-cell signaling, transmembrane transport, behavior and organism process (Biological process); metal ion transmembrane transporter activity and neuropeptide hormone activity (Molecular function). Enrichment analysis identified 40 KEGG significantly enriched pathways. Based on the observation of the lowest P-value, calcium, oxytocin, circadian entrainment, cholinergic glutamatergic, dopaminergic, serotonergic and GABAergic synapse, GnRH, Estrogen, Rap1, MAPK, ErbB, Ras and cancer signaling and several drugs addiction were among the most important enriched pathways. At the top list the DGs we found a group of genes including the homeodomain transcription factor Orthopedia OTP and the coregulated genes AVP, OXT, CRH and TH known for their physiological roles associated with lactation and mammalian social behaviors.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/174863
Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves; 36th International Society for Animal Genetics Conference; Dublin; Irlanda; 2017; 48-49
CONICET Digital
CONICET
url http://hdl.handle.net/11336/174863
identifier_str_mv Hypothalamus transcriptome during the early rise in LH secretion related to puberty age in bull calves; 36th International Society for Animal Genetics Conference; Dublin; Irlanda; 2017; 48-49
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.isag.us/2017/
info:eu-repo/semantics/altIdentifier/url/https://www.isag.us/Docs/Proceedings/ISAG2017_Proceedings.pdf?v3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv International Society for Animal Genetics
publisher.none.fl_str_mv International Society for Animal Genetics
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083452144713728
score 13.22299

Publicaciones similares