Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins

Autores
Laino, Aldana; Cunningham, Monica Liliana; Costa, Fernando; Garcia, Carlos Fernando
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In oviparous species, proteins and lipids found in the vitellus form the lipoproteins called lipovitellins that are the major source of energy for the development, growth, and survival of the embryo. The energy resources provided by the lipovitellins have not yet been investigated in the Order Araneae. Using the wolf spider Schizocosa malitiosa (Lycosidae) as an experimental model, we identified and characterized the lipovitellins present in the cytosol, focusing on the energetic contribution of those lipoprotein particles in the vitellus. Two lipovitellins (LV) named SmLV1 and SmLV2 were isolated. SmLV1 is a high-density lipoprotein with 67% lipid and 3.6% carbohydrate, and SmLV2 is a very high-density lipoprotein with 9% lipid and 8.8% carbohydrate. Through electrophoresis in native conditions we observed that SmLV1 has a molecular mass of 559 kDa composed of three apolipoproteins of 116, 87, and 42 kDa, respectively. SmLV2 comprised several proteins composed of different proportions of the same subunits (135, 126, 109, and 70 kDa). The principal lipids of these lipovitellins are sphingomyelin + lysophosphatidylcholine, esterified sterols, and phosphatidylcholine. Lipovitellin-free cytosol contains abundant phospatidylcholine and triacylglyceride related to the yolk nuclei (the vitellus organizing center). The principal fatty acids of SmLV1 and SmLV2 are 18:2 n−6, 18:1 n−9, and 16:0. Spectrophotometry detected no pigments in either the lipovitellins or the cytosol. The egg caloric content was 92 cal/g, at proportions of 59.8% protein, 20.1% carbohydrate, and 19.9% lipid. SmLV1 and SmLV2 provided 19.5% and 17.1% of the calories, respectively. Both lipovitellins contribute mainly with proteins (15.8–18%), with the input of carbohydrates and lipids being lower than 1.3%.
Fil: Laino, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Cunningham, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Costa, Fernando. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Garcia, Carlos Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Materia
Lipovitellins
Arachnids
Lipids
Fatty Acid
Energy Sources
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23930
Acceder
id CONICETDig_41831d92f807a4e9712339dc6ca4303c
oai_identifier_str oai:ri.conicet.gov.ar:11336/23930
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellinsLaino, AldanaCunningham, Monica LilianaCosta, FernandoGarcia, Carlos FernandoLipovitellinsArachnidsLipidsFatty AcidEnergy SourcesIn oviparous species, proteins and lipids found in the vitellus form the lipoproteins called lipovitellins that are the major source of energy for the development, growth, and survival of the embryo. The energy resources provided by the lipovitellins have not yet been investigated in the Order Araneae. Using the wolf spider Schizocosa malitiosa (Lycosidae) as an experimental model, we identified and characterized the lipovitellins present in the cytosol, focusing on the energetic contribution of those lipoprotein particles in the vitellus. Two lipovitellins (LV) named SmLV1 and SmLV2 were isolated. SmLV1 is a high-density lipoprotein with 67% lipid and 3.6% carbohydrate, and SmLV2 is a very high-density lipoprotein with 9% lipid and 8.8% carbohydrate. Through electrophoresis in native conditions we observed that SmLV1 has a molecular mass of 559 kDa composed of three apolipoproteins of 116, 87, and 42 kDa, respectively. SmLV2 comprised several proteins composed of different proportions of the same subunits (135, 126, 109, and 70 kDa). The principal lipids of these lipovitellins are sphingomyelin + lysophosphatidylcholine, esterified sterols, and phosphatidylcholine. Lipovitellin-free cytosol contains abundant phospatidylcholine and triacylglyceride related to the yolk nuclei (the vitellus organizing center). The principal fatty acids of SmLV1 and SmLV2 are 18:2 n−6, 18:1 n−9, and 16:0. Spectrophotometry detected no pigments in either the lipovitellins or the cytosol. The egg caloric content was 92 cal/g, at proportions of 59.8% protein, 20.1% carbohydrate, and 19.9% lipid. SmLV1 and SmLV2 provided 19.5% and 17.1% of the calories, respectively. Both lipovitellins contribute mainly with proteins (15.8–18%), with the input of carbohydrates and lipids being lower than 1.3%.Fil: Laino, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Cunningham, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Costa, Fernando. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Garcia, Carlos Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaElsevier Science Inc2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23930Laino, Aldana; Cunningham, Monica Liliana; Costa, Fernando; Garcia, Carlos Fernando; Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins; Elsevier Science Inc; Comparative Biochemistry And Physiology. Part B, Biochemistry & Molecular Biology.; 165; 3; 4-2013; 172-1801096-4959CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S109649591300064Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpb.2013.04.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:53:59Zoai:ri.conicet.gov.ar:11336/23930instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:53:59.943CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins
title Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins
spellingShingle Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins
Laino, Aldana
Lipovitellins
Arachnids
Lipids
Fatty Acid
Energy Sources
title_short Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins
title_full Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins
title_fullStr Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins
title_full_unstemmed Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins
title_sort Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins
dc.creator.none.fl_str_mv Laino, Aldana
Cunningham, Monica Liliana
Costa, Fernando
Garcia, Carlos Fernando
author Laino, Aldana
author_facet Laino, Aldana
Cunningham, Monica Liliana
Costa, Fernando
Garcia, Carlos Fernando
author_role author
author2 Cunningham, Monica Liliana
Costa, Fernando
Garcia, Carlos Fernando
author2_role author
author
author
dc.subject.none.fl_str_mv Lipovitellins
Arachnids
Lipids
Fatty Acid
Energy Sources
topic Lipovitellins
Arachnids
Lipids
Fatty Acid
Energy Sources
dc.description.none.fl_txt_mv In oviparous species, proteins and lipids found in the vitellus form the lipoproteins called lipovitellins that are the major source of energy for the development, growth, and survival of the embryo. The energy resources provided by the lipovitellins have not yet been investigated in the Order Araneae. Using the wolf spider Schizocosa malitiosa (Lycosidae) as an experimental model, we identified and characterized the lipovitellins present in the cytosol, focusing on the energetic contribution of those lipoprotein particles in the vitellus. Two lipovitellins (LV) named SmLV1 and SmLV2 were isolated. SmLV1 is a high-density lipoprotein with 67% lipid and 3.6% carbohydrate, and SmLV2 is a very high-density lipoprotein with 9% lipid and 8.8% carbohydrate. Through electrophoresis in native conditions we observed that SmLV1 has a molecular mass of 559 kDa composed of three apolipoproteins of 116, 87, and 42 kDa, respectively. SmLV2 comprised several proteins composed of different proportions of the same subunits (135, 126, 109, and 70 kDa). The principal lipids of these lipovitellins are sphingomyelin + lysophosphatidylcholine, esterified sterols, and phosphatidylcholine. Lipovitellin-free cytosol contains abundant phospatidylcholine and triacylglyceride related to the yolk nuclei (the vitellus organizing center). The principal fatty acids of SmLV1 and SmLV2 are 18:2 n−6, 18:1 n−9, and 16:0. Spectrophotometry detected no pigments in either the lipovitellins or the cytosol. The egg caloric content was 92 cal/g, at proportions of 59.8% protein, 20.1% carbohydrate, and 19.9% lipid. SmLV1 and SmLV2 provided 19.5% and 17.1% of the calories, respectively. Both lipovitellins contribute mainly with proteins (15.8–18%), with the input of carbohydrates and lipids being lower than 1.3%.
Fil: Laino, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Cunningham, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Costa, Fernando. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Garcia, Carlos Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
description In oviparous species, proteins and lipids found in the vitellus form the lipoproteins called lipovitellins that are the major source of energy for the development, growth, and survival of the embryo. The energy resources provided by the lipovitellins have not yet been investigated in the Order Araneae. Using the wolf spider Schizocosa malitiosa (Lycosidae) as an experimental model, we identified and characterized the lipovitellins present in the cytosol, focusing on the energetic contribution of those lipoprotein particles in the vitellus. Two lipovitellins (LV) named SmLV1 and SmLV2 were isolated. SmLV1 is a high-density lipoprotein with 67% lipid and 3.6% carbohydrate, and SmLV2 is a very high-density lipoprotein with 9% lipid and 8.8% carbohydrate. Through electrophoresis in native conditions we observed that SmLV1 has a molecular mass of 559 kDa composed of three apolipoproteins of 116, 87, and 42 kDa, respectively. SmLV2 comprised several proteins composed of different proportions of the same subunits (135, 126, 109, and 70 kDa). The principal lipids of these lipovitellins are sphingomyelin + lysophosphatidylcholine, esterified sterols, and phosphatidylcholine. Lipovitellin-free cytosol contains abundant phospatidylcholine and triacylglyceride related to the yolk nuclei (the vitellus organizing center). The principal fatty acids of SmLV1 and SmLV2 are 18:2 n−6, 18:1 n−9, and 16:0. Spectrophotometry detected no pigments in either the lipovitellins or the cytosol. The egg caloric content was 92 cal/g, at proportions of 59.8% protein, 20.1% carbohydrate, and 19.9% lipid. SmLV1 and SmLV2 provided 19.5% and 17.1% of the calories, respectively. Both lipovitellins contribute mainly with proteins (15.8–18%), with the input of carbohydrates and lipids being lower than 1.3%.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23930
Laino, Aldana; Cunningham, Monica Liliana; Costa, Fernando; Garcia, Carlos Fernando; Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins; Elsevier Science Inc; Comparative Biochemistry And Physiology. Part B, Biochemistry & Molecular Biology.; 165; 3; 4-2013; 172-180
1096-4959
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23930
identifier_str_mv Laino, Aldana; Cunningham, Monica Liliana; Costa, Fernando; Garcia, Carlos Fernando; Energy sources from the eggs of the wolf spider Schizocosa malitiosa: Isolation and characterization of lipovitellins; Elsevier Science Inc; Comparative Biochemistry And Physiology. Part B, Biochemistry & Molecular Biology.; 165; 3; 4-2013; 172-180
1096-4959
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S109649591300064X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpb.2013.04.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782236991422464
score 12.982451

Publicaciones similares