In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage

Autores
Casanova, Natalia Andrea; Ariagno, Julia Irene; López Nigro, Marcela Mabel; Mendeluk, Gabriela Ruth; Gette, Maria de Los Angeles; Petenatti, Elisa Margarita; Palaoro, Luis Alberto; Carballo, Marta Ana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study was carried out to investigate the genotoxicity as well as possible protective activity against damage induced by cyclophosphamide (CP) of the aqueous juice of watercress (Nasturtium officinale, W.T. Aiton) in vivo. Male and female Swiss mice 7-8 weeks old (N = 48) were treated by gavage with 1 g kg(-1) body weight and 0.5 g kg(-1) body weight of watercress juice during 15 consecutive days. Genotoxicity and its possible protective effect were tested by the comet assay in peripheral blood cells and the micronucleus test in bone marrow. In addition, biopsies of the bladder, epididymis and testicles of mice were performed to extend the experimental design. Watercress juice per se did not induce genetic damage according to the comet assay and micronucleus study, exhibiting a protective activity against CP (P < 0.05 and P < 0.001, respectively). The comparative analysis of bladder histological changes obtained in the watercress plus CP group against those treated with CP alone suggests a probable protective effect. Further studies are needed in order to establish the protective role of watercress juice against DNA damage.
Fil: Casanova, Natalia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ariagno, Julia Irene. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: López Nigro, Marcela Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Mendeluk, Gabriela Ruth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Gette, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Petenatti, Elisa Margarita. Universidad Nacional de San Luis; Argentina
Fil: Palaoro, Luis Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Carballo, Marta Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Materia
antigenotoxicity
biomarkers
bladder
cyclophosphamide
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109256
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/109256
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damageCasanova, Natalia AndreaAriagno, Julia IreneLópez Nigro, Marcela MabelMendeluk, Gabriela RuthGette, Maria de Los AngelesPetenatti, Elisa MargaritaPalaoro, Luis AlbertoCarballo, Marta Anaantigenotoxicitybiomarkersbladdercyclophosphamidehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The present study was carried out to investigate the genotoxicity as well as possible protective activity against damage induced by cyclophosphamide (CP) of the aqueous juice of watercress (Nasturtium officinale, W.T. Aiton) in vivo. Male and female Swiss mice 7-8 weeks old (N = 48) were treated by gavage with 1 g kg(-1) body weight and 0.5 g kg(-1) body weight of watercress juice during 15 consecutive days. Genotoxicity and its possible protective effect were tested by the comet assay in peripheral blood cells and the micronucleus test in bone marrow. In addition, biopsies of the bladder, epididymis and testicles of mice were performed to extend the experimental design. Watercress juice per se did not induce genetic damage according to the comet assay and micronucleus study, exhibiting a protective activity against CP (P < 0.05 and P < 0.001, respectively). The comparative analysis of bladder histological changes obtained in the watercress plus CP group against those treated with CP alone suggests a probable protective effect. Further studies are needed in order to establish the protective role of watercress juice against DNA damage.Fil: Casanova, Natalia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ariagno, Julia Irene. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: López Nigro, Marcela Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Mendeluk, Gabriela Ruth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Gette, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Petenatti, Elisa Margarita. Universidad Nacional de San Luis; ArgentinaFil: Palaoro, Luis Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Carballo, Marta Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaJohn Wiley & Sons Ltd2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109256Casanova, Natalia Andrea; Ariagno, Julia Irene; López Nigro, Marcela Mabel; Mendeluk, Gabriela Ruth; Gette, Maria de Los Angeles; et al.; In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage; John Wiley & Sons Ltd; Journal of Applied Toxicology; 33; 9; 9-2013; 880-8850260-437XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jat.2746info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jat.2746info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:33:34Zoai:ri.conicet.gov.ar:11336/109256instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:33:34.529CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage
title In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage
spellingShingle In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage
Casanova, Natalia Andrea
antigenotoxicity
biomarkers
bladder
cyclophosphamide
title_short In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage
title_full In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage
title_fullStr In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage
title_full_unstemmed In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage
title_sort In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage
dc.creator.none.fl_str_mv Casanova, Natalia Andrea
Ariagno, Julia Irene
López Nigro, Marcela Mabel
Mendeluk, Gabriela Ruth
Gette, Maria de Los Angeles
Petenatti, Elisa Margarita
Palaoro, Luis Alberto
Carballo, Marta Ana
author Casanova, Natalia Andrea
author_facet Casanova, Natalia Andrea
Ariagno, Julia Irene
López Nigro, Marcela Mabel
Mendeluk, Gabriela Ruth
Gette, Maria de Los Angeles
Petenatti, Elisa Margarita
Palaoro, Luis Alberto
Carballo, Marta Ana
author_role author
author2 Ariagno, Julia Irene
López Nigro, Marcela Mabel
Mendeluk, Gabriela Ruth
Gette, Maria de Los Angeles
Petenatti, Elisa Margarita
Palaoro, Luis Alberto
Carballo, Marta Ana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv antigenotoxicity
biomarkers
bladder
cyclophosphamide
topic antigenotoxicity
biomarkers
bladder
cyclophosphamide
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The present study was carried out to investigate the genotoxicity as well as possible protective activity against damage induced by cyclophosphamide (CP) of the aqueous juice of watercress (Nasturtium officinale, W.T. Aiton) in vivo. Male and female Swiss mice 7-8 weeks old (N = 48) were treated by gavage with 1 g kg(-1) body weight and 0.5 g kg(-1) body weight of watercress juice during 15 consecutive days. Genotoxicity and its possible protective effect were tested by the comet assay in peripheral blood cells and the micronucleus test in bone marrow. In addition, biopsies of the bladder, epididymis and testicles of mice were performed to extend the experimental design. Watercress juice per se did not induce genetic damage according to the comet assay and micronucleus study, exhibiting a protective activity against CP (P < 0.05 and P < 0.001, respectively). The comparative analysis of bladder histological changes obtained in the watercress plus CP group against those treated with CP alone suggests a probable protective effect. Further studies are needed in order to establish the protective role of watercress juice against DNA damage.
Fil: Casanova, Natalia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ariagno, Julia Irene. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: López Nigro, Marcela Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Mendeluk, Gabriela Ruth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Gette, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Petenatti, Elisa Margarita. Universidad Nacional de San Luis; Argentina
Fil: Palaoro, Luis Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Carballo, Marta Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
description The present study was carried out to investigate the genotoxicity as well as possible protective activity against damage induced by cyclophosphamide (CP) of the aqueous juice of watercress (Nasturtium officinale, W.T. Aiton) in vivo. Male and female Swiss mice 7-8 weeks old (N = 48) were treated by gavage with 1 g kg(-1) body weight and 0.5 g kg(-1) body weight of watercress juice during 15 consecutive days. Genotoxicity and its possible protective effect were tested by the comet assay in peripheral blood cells and the micronucleus test in bone marrow. In addition, biopsies of the bladder, epididymis and testicles of mice were performed to extend the experimental design. Watercress juice per se did not induce genetic damage according to the comet assay and micronucleus study, exhibiting a protective activity against CP (P < 0.05 and P < 0.001, respectively). The comparative analysis of bladder histological changes obtained in the watercress plus CP group against those treated with CP alone suggests a probable protective effect. Further studies are needed in order to establish the protective role of watercress juice against DNA damage.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109256
Casanova, Natalia Andrea; Ariagno, Julia Irene; López Nigro, Marcela Mabel; Mendeluk, Gabriela Ruth; Gette, Maria de Los Angeles; et al.; In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage; John Wiley & Sons Ltd; Journal of Applied Toxicology; 33; 9; 9-2013; 880-885
0260-437X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109256
identifier_str_mv Casanova, Natalia Andrea; Ariagno, Julia Irene; López Nigro, Marcela Mabel; Mendeluk, Gabriela Ruth; Gette, Maria de Los Angeles; et al.; In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage; John Wiley & Sons Ltd; Journal of Applied Toxicology; 33; 9; 9-2013; 880-885
0260-437X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jat.2746
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jat.2746
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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