Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat

Autores
Larran, Silvina; Siurana, María Pilar Santamarina; Caselles, Josefa Roselló; Simón, María Rosa; Perello, Analia Edith
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An endophytic fungus isolated from healthy wheat seeds cultivar Klein Yarará in Buenos Aires Province, Argentina, was initially identified as belonging to the Fusarium fujikuroi species complex based on morphological and cultural characteristics. The fungus role in pathogenicity was investigated by artificial inoculation of wheat seeds. Symptoms evaluated 7 and 14 days after inoculation showed that the fungus was pathogenic on seed and seedlings causing symptoms as seed decay, seedling blight and seed rot. The fungus was re-isolated to fulfill Koch`s Postulates and was identified as F. sudanense (strain LBEA 3100), a new species recorded in Argentina. The fungal identity was corroborated using molecular techniques by sequencing the ITS region, D1/D2 domains of the LSU gene and TEF-1 alpha region and by comparison with international databases. Ecophysiological studies of F. sudanense (LBEA 3100) performed at different water activities and temperatures showed faster growth rate at the highest water activity and 25 ºC. This is the first report of F. sudanense, isolated from healthy wheat seeds, causing typical symptoms of seedling blight and seed rot on wheat.
Fil: Larran, Silvina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Siurana, María Pilar Santamarina. Universidad Politécnica de Valencia; España
Fil: Caselles, Josefa Roselló. Universidad Politécnica de Valencia; España
Fil: Simón, María Rosa. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Tecnología Agropecuaria y Forestal; Argentina
Fil: Perello, Analia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; Argentina
Materia
FUSARIUM SUDANENSE
TRITICUM AESTIVUM
ENDOPHYTIC FUNGUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104504
Acceder
id CONICETDig_4154863a9ea5a8de75b131e5b5cd208e
oai_identifier_str oai:ri.conicet.gov.ar:11336/104504
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheatLarran, SilvinaSiurana, María Pilar SantamarinaCaselles, Josefa RosellóSimón, María RosaPerello, Analia EdithFUSARIUM SUDANENSETRITICUM AESTIVUMENDOPHYTIC FUNGUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An endophytic fungus isolated from healthy wheat seeds cultivar Klein Yarará in Buenos Aires Province, Argentina, was initially identified as belonging to the Fusarium fujikuroi species complex based on morphological and cultural characteristics. The fungus role in pathogenicity was investigated by artificial inoculation of wheat seeds. Symptoms evaluated 7 and 14 days after inoculation showed that the fungus was pathogenic on seed and seedlings causing symptoms as seed decay, seedling blight and seed rot. The fungus was re-isolated to fulfill Koch`s Postulates and was identified as F. sudanense (strain LBEA 3100), a new species recorded in Argentina. The fungal identity was corroborated using molecular techniques by sequencing the ITS region, D1/D2 domains of the LSU gene and TEF-1 alpha region and by comparison with international databases. Ecophysiological studies of F. sudanense (LBEA 3100) performed at different water activities and temperatures showed faster growth rate at the highest water activity and 25 ºC. This is the first report of F. sudanense, isolated from healthy wheat seeds, causing typical symptoms of seedling blight and seed rot on wheat.Fil: Larran, Silvina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaFil: Siurana, María Pilar Santamarina. Universidad Politécnica de Valencia; EspañaFil: Caselles, Josefa Roselló. Universidad Politécnica de Valencia; EspañaFil: Simón, María Rosa. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Tecnología Agropecuaria y Forestal; ArgentinaFil: Perello, Analia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; ArgentinaElsevier2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104504Larran, Silvina; Siurana, María Pilar Santamarina; Caselles, Josefa Roselló; Simón, María Rosa; Perello, Analia Edith; Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat; Elsevier; Journal of King Saud University - Science; 32; 1; 7-2018; 468-4741018-3647CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1018364718307365info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jksus.2018.07.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:40:34Zoai:ri.conicet.gov.ar:11336/104504instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:40:35.046CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat
title Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat
spellingShingle Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat
Larran, Silvina
FUSARIUM SUDANENSE
TRITICUM AESTIVUM
ENDOPHYTIC FUNGUS
title_short Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat
title_full Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat
title_fullStr Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat
title_full_unstemmed Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat
title_sort Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat
dc.creator.none.fl_str_mv Larran, Silvina
Siurana, María Pilar Santamarina
Caselles, Josefa Roselló
Simón, María Rosa
Perello, Analia Edith
author Larran, Silvina
author_facet Larran, Silvina
Siurana, María Pilar Santamarina
Caselles, Josefa Roselló
Simón, María Rosa
Perello, Analia Edith
author_role author
author2 Siurana, María Pilar Santamarina
Caselles, Josefa Roselló
Simón, María Rosa
Perello, Analia Edith
author2_role author
author
author
author
dc.subject.none.fl_str_mv FUSARIUM SUDANENSE
TRITICUM AESTIVUM
ENDOPHYTIC FUNGUS
topic FUSARIUM SUDANENSE
TRITICUM AESTIVUM
ENDOPHYTIC FUNGUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An endophytic fungus isolated from healthy wheat seeds cultivar Klein Yarará in Buenos Aires Province, Argentina, was initially identified as belonging to the Fusarium fujikuroi species complex based on morphological and cultural characteristics. The fungus role in pathogenicity was investigated by artificial inoculation of wheat seeds. Symptoms evaluated 7 and 14 days after inoculation showed that the fungus was pathogenic on seed and seedlings causing symptoms as seed decay, seedling blight and seed rot. The fungus was re-isolated to fulfill Koch`s Postulates and was identified as F. sudanense (strain LBEA 3100), a new species recorded in Argentina. The fungal identity was corroborated using molecular techniques by sequencing the ITS region, D1/D2 domains of the LSU gene and TEF-1 alpha region and by comparison with international databases. Ecophysiological studies of F. sudanense (LBEA 3100) performed at different water activities and temperatures showed faster growth rate at the highest water activity and 25 ºC. This is the first report of F. sudanense, isolated from healthy wheat seeds, causing typical symptoms of seedling blight and seed rot on wheat.
Fil: Larran, Silvina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Siurana, María Pilar Santamarina. Universidad Politécnica de Valencia; España
Fil: Caselles, Josefa Roselló. Universidad Politécnica de Valencia; España
Fil: Simón, María Rosa. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Tecnología Agropecuaria y Forestal; Argentina
Fil: Perello, Analia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; Argentina
description An endophytic fungus isolated from healthy wheat seeds cultivar Klein Yarará in Buenos Aires Province, Argentina, was initially identified as belonging to the Fusarium fujikuroi species complex based on morphological and cultural characteristics. The fungus role in pathogenicity was investigated by artificial inoculation of wheat seeds. Symptoms evaluated 7 and 14 days after inoculation showed that the fungus was pathogenic on seed and seedlings causing symptoms as seed decay, seedling blight and seed rot. The fungus was re-isolated to fulfill Koch`s Postulates and was identified as F. sudanense (strain LBEA 3100), a new species recorded in Argentina. The fungal identity was corroborated using molecular techniques by sequencing the ITS region, D1/D2 domains of the LSU gene and TEF-1 alpha region and by comparison with international databases. Ecophysiological studies of F. sudanense (LBEA 3100) performed at different water activities and temperatures showed faster growth rate at the highest water activity and 25 ºC. This is the first report of F. sudanense, isolated from healthy wheat seeds, causing typical symptoms of seedling blight and seed rot on wheat.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104504
Larran, Silvina; Siurana, María Pilar Santamarina; Caselles, Josefa Roselló; Simón, María Rosa; Perello, Analia Edith; Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat; Elsevier; Journal of King Saud University - Science; 32; 1; 7-2018; 468-474
1018-3647
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104504
identifier_str_mv Larran, Silvina; Siurana, María Pilar Santamarina; Caselles, Josefa Roselló; Simón, María Rosa; Perello, Analia Edith; Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat; Elsevier; Journal of King Saud University - Science; 32; 1; 7-2018; 468-474
1018-3647
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1018364718307365
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jksus.2018.07.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082898187255808
score 13.22299

Publicaciones similares