Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana

Autores
García Bossi, Julián; Obertello, Mariana; Barberini, María Laura; Salinas, Nicolás; Estevez, Jose Manuel; Muschietti, Jorge Prometeo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In plants, calcium signals are involved in multiple physiologicalprocesses such as stomatal opening, stress responses, and polarized growth ofroot hairs and pollen tubes. These signals are given as repetitive oscillationsof cytosolic free Ca2+ where the intensity and amplitude correlateaccording to the stimulus. Pollen tube growth occurs through the concertedaction of different factors such as pH, ROS, actin and calcium gradient. Anyimbalance between these factors causes aborted pollen tubes and therefore, defectsin fertility.In this work, we propose to perform a functional study of the P2B typecalcium pumps (Autoinhibited Ca2+-ATPases,ACAs), in pollen tubes of Arabidopsis thaliana. These pumps are mainlylocated in vacuole, endoplasmic reticulum and/or plasma membrane and areinvolved in removing calcium from the cytoplasm. Studies conducted with ACAinsertional mutants have shown that these pumps have a role beyond themaintenance of Ca2+ homeostasis. Since some Arabidopsis ACAs arespecifically expressed in mature pollen, we propose to explore pollen-pistilinteractions of ACA mutant plants.  Wewill also study calcium dynamics during pollen tube growth. These results will provide new perspectivesabout how pollen tube growth is regulated and it will be also applicable toother cellular polar growth models.
Fil: García Bossi, Julián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Obertello, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Barberini, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Salinas, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Salta
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Panamerican Association of Biochemestry and Molecular Biology
Materia
POLLEN TUBE
ARABIDOPSIS
CALCIUM
ACAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153745
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/153745
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of calcium transporters during the pollen tube growth in Arabidopsis thalianaGarcía Bossi, JuliánObertello, MarianaBarberini, María LauraSalinas, NicolásEstevez, Jose ManuelMuschietti, Jorge PrometeoPOLLEN TUBEARABIDOPSISCALCIUMACAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In plants, calcium signals are involved in multiple physiologicalprocesses such as stomatal opening, stress responses, and polarized growth ofroot hairs and pollen tubes. These signals are given as repetitive oscillationsof cytosolic free Ca2+ where the intensity and amplitude correlateaccording to the stimulus. Pollen tube growth occurs through the concertedaction of different factors such as pH, ROS, actin and calcium gradient. Anyimbalance between these factors causes aborted pollen tubes and therefore, defectsin fertility.In this work, we propose to perform a functional study of the P2B typecalcium pumps (Autoinhibited Ca2+-ATPases,ACAs), in pollen tubes of Arabidopsis thaliana. These pumps are mainlylocated in vacuole, endoplasmic reticulum and/or plasma membrane and areinvolved in removing calcium from the cytoplasm. Studies conducted with ACAinsertional mutants have shown that these pumps have a role beyond themaintenance of Ca2+ homeostasis. Since some Arabidopsis ACAs arespecifically expressed in mature pollen, we propose to explore pollen-pistilinteractions of ACA mutant plants.  Wewill also study calcium dynamics during pollen tube growth. These results will provide new perspectivesabout how pollen tube growth is regulated and it will be also applicable toother cellular polar growth models.Fil: García Bossi, Julián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Obertello, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Barberini, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Salinas, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaThe LV Annual SAIB Meeting and XIV PABMB CongressSaltaArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularPanamerican Association of Biochemestry and Molecular BiologyTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153745Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-60327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdfinfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v43nSuppl.5/33868Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:46Zoai:ri.conicet.gov.ar:11336/153745instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:47.087CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana
title Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana
spellingShingle Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana
García Bossi, Julián
POLLEN TUBE
ARABIDOPSIS
CALCIUM
ACAS
title_short Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana
title_full Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana
title_fullStr Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana
title_full_unstemmed Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana
title_sort Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana
dc.creator.none.fl_str_mv García Bossi, Julián
Obertello, Mariana
Barberini, María Laura
Salinas, Nicolás
Estevez, Jose Manuel
Muschietti, Jorge Prometeo
author García Bossi, Julián
author_facet García Bossi, Julián
Obertello, Mariana
Barberini, María Laura
Salinas, Nicolás
Estevez, Jose Manuel
Muschietti, Jorge Prometeo
author_role author
author2 Obertello, Mariana
Barberini, María Laura
Salinas, Nicolás
Estevez, Jose Manuel
Muschietti, Jorge Prometeo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv POLLEN TUBE
ARABIDOPSIS
CALCIUM
ACAS
topic POLLEN TUBE
ARABIDOPSIS
CALCIUM
ACAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In plants, calcium signals are involved in multiple physiologicalprocesses such as stomatal opening, stress responses, and polarized growth ofroot hairs and pollen tubes. These signals are given as repetitive oscillationsof cytosolic free Ca2+ where the intensity and amplitude correlateaccording to the stimulus. Pollen tube growth occurs through the concertedaction of different factors such as pH, ROS, actin and calcium gradient. Anyimbalance between these factors causes aborted pollen tubes and therefore, defectsin fertility.In this work, we propose to perform a functional study of the P2B typecalcium pumps (Autoinhibited Ca2+-ATPases,ACAs), in pollen tubes of Arabidopsis thaliana. These pumps are mainlylocated in vacuole, endoplasmic reticulum and/or plasma membrane and areinvolved in removing calcium from the cytoplasm. Studies conducted with ACAinsertional mutants have shown that these pumps have a role beyond themaintenance of Ca2+ homeostasis. Since some Arabidopsis ACAs arespecifically expressed in mature pollen, we propose to explore pollen-pistilinteractions of ACA mutant plants.  Wewill also study calcium dynamics during pollen tube growth. These results will provide new perspectivesabout how pollen tube growth is regulated and it will be also applicable toother cellular polar growth models.
Fil: García Bossi, Julián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Obertello, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Barberini, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Salinas, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Salta
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Panamerican Association of Biochemestry and Molecular Biology
description In plants, calcium signals are involved in multiple physiologicalprocesses such as stomatal opening, stress responses, and polarized growth ofroot hairs and pollen tubes. These signals are given as repetitive oscillationsof cytosolic free Ca2+ where the intensity and amplitude correlateaccording to the stimulus. Pollen tube growth occurs through the concertedaction of different factors such as pH, ROS, actin and calcium gradient. Anyimbalance between these factors causes aborted pollen tubes and therefore, defectsin fertility.In this work, we propose to perform a functional study of the P2B typecalcium pumps (Autoinhibited Ca2+-ATPases,ACAs), in pollen tubes of Arabidopsis thaliana. These pumps are mainlylocated in vacuole, endoplasmic reticulum and/or plasma membrane and areinvolved in removing calcium from the cytoplasm. Studies conducted with ACAinsertional mutants have shown that these pumps have a role beyond themaintenance of Ca2+ homeostasis. Since some Arabidopsis ACAs arespecifically expressed in mature pollen, we propose to explore pollen-pistilinteractions of ACA mutant plants.  Wewill also study calcium dynamics during pollen tube growth. These results will provide new perspectivesabout how pollen tube growth is regulated and it will be also applicable toother cellular polar growth models.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153745
Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-6
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153745
identifier_str_mv Characterization of calcium transporters during the pollen tube growth in Arabidopsis thaliana; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-6
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdf
info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v43nSuppl.5/33868
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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