Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome

Autores
Ferreira, Sandra María; Lerner, S.F; Brunzini, R; Evelson, Pablo Andrés; Llesuy, Susana Francisca
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
PURPOSE: To establish the antioxidant status of the aqueous humour in glaucoma associated with exfoliation syndrome (XFG) and to compare it to primary open-angle glaucoma (POAG) and cataract patients. METHODS: Patients were diagnosed with POAG, XFG, or cataract (n=25 for each group). Total reactive antioxidant potential (TRAP) was measured by chemiluminescence. Ascorbic acid levels and the activities of catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD) were measured spectrophotometrically.ResultsTRAP value was lower in XFG (28+/-2 microM Trolox) than in POAG (55+/-8 microM Trolox; P<0.001). TRAP values in both glaucomas were lower than the cataract value (124+/-5 microM Trolox; P<0.001). A decrease in ascorbic acid was measured in XFG (230+/-20 microM) compared with POAG (415+/-17 microM; P<0.001). Ascorbic acid in both glaucomas was lower than in cataract (720+/-30 microM; P<0.001). A significant increase in GPx was found in XFG (30+/-2 U/ml) compared with POAG (16+/-3 U/ml). GPx activity in both glaucomas was increased when compared with cataracts (6+/-2 U/ml; P<0.001). A significant increase of 67% in SOD activity was observed in the glaucoma group vscataract group (27+/-3 U/ml; P<0.001), but no changes were found between both glaucomas. CONCLUSIONS: The antioxidant status of the aqueous humour may play a role in the pathophysiology of both glaucomas.
Fil: Ferreira, Sandra María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Lerner, S.F. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Brunzini, R. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Evelson, Pablo Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Llesuy, Susana Francisca. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
OXIDATIVE STRESS
AQUEOUS HUMOUR
ANTIOXIDANTS
GLAUCOMA ASSOCIATED WITH EXFOLIATION SYNDROME
PRIMARY OPEN ANGLE GLAUCOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102636
Acceder
id CONICETDig_407b035611c50ffc19970ed81bd0d94f
oai_identifier_str oai:ri.conicet.gov.ar:11336/102636
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndromeFerreira, Sandra MaríaLerner, S.FBrunzini, REvelson, Pablo AndrésLlesuy, Susana FranciscaOXIDATIVE STRESSAQUEOUS HUMOURANTIOXIDANTSGLAUCOMA ASSOCIATED WITH EXFOLIATION SYNDROMEPRIMARY OPEN ANGLE GLAUCOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1PURPOSE: To establish the antioxidant status of the aqueous humour in glaucoma associated with exfoliation syndrome (XFG) and to compare it to primary open-angle glaucoma (POAG) and cataract patients. METHODS: Patients were diagnosed with POAG, XFG, or cataract (n=25 for each group). Total reactive antioxidant potential (TRAP) was measured by chemiluminescence. Ascorbic acid levels and the activities of catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD) were measured spectrophotometrically.ResultsTRAP value was lower in XFG (28+/-2 microM Trolox) than in POAG (55+/-8 microM Trolox; P<0.001). TRAP values in both glaucomas were lower than the cataract value (124+/-5 microM Trolox; P<0.001). A decrease in ascorbic acid was measured in XFG (230+/-20 microM) compared with POAG (415+/-17 microM; P<0.001). Ascorbic acid in both glaucomas was lower than in cataract (720+/-30 microM; P<0.001). A significant increase in GPx was found in XFG (30+/-2 U/ml) compared with POAG (16+/-3 U/ml). GPx activity in both glaucomas was increased when compared with cataracts (6+/-2 U/ml; P<0.001). A significant increase of 67% in SOD activity was observed in the glaucoma group vscataract group (27+/-3 U/ml; P<0.001), but no changes were found between both glaucomas. CONCLUSIONS: The antioxidant status of the aqueous humour may play a role in the pathophysiology of both glaucomas.Fil: Ferreira, Sandra María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; ArgentinaFil: Lerner, S.F. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; ArgentinaFil: Brunzini, R. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; ArgentinaFil: Evelson, Pablo Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Llesuy, Susana Francisca. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaNature Publishing Group2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102636Ferreira, Sandra María; Lerner, S.F; Brunzini, R; Evelson, Pablo Andrés; Llesuy, Susana Francisca; Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome; Nature Publishing Group; Eye; 23; 12-2009; 1691-16970950-222XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/eye2008352?draft=marketinginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:00Zoai:ri.conicet.gov.ar:11336/102636instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:01.271CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome
title Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome
spellingShingle Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome
Ferreira, Sandra María
OXIDATIVE STRESS
AQUEOUS HUMOUR
ANTIOXIDANTS
GLAUCOMA ASSOCIATED WITH EXFOLIATION SYNDROME
PRIMARY OPEN ANGLE GLAUCOME
title_short Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome
title_full Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome
title_fullStr Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome
title_full_unstemmed Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome
title_sort Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome
dc.creator.none.fl_str_mv Ferreira, Sandra María
Lerner, S.F
Brunzini, R
Evelson, Pablo Andrés
Llesuy, Susana Francisca
author Ferreira, Sandra María
author_facet Ferreira, Sandra María
Lerner, S.F
Brunzini, R
Evelson, Pablo Andrés
Llesuy, Susana Francisca
author_role author
author2 Lerner, S.F
Brunzini, R
Evelson, Pablo Andrés
Llesuy, Susana Francisca
author2_role author
author
author
author
dc.subject.none.fl_str_mv OXIDATIVE STRESS
AQUEOUS HUMOUR
ANTIOXIDANTS
GLAUCOMA ASSOCIATED WITH EXFOLIATION SYNDROME
PRIMARY OPEN ANGLE GLAUCOME
topic OXIDATIVE STRESS
AQUEOUS HUMOUR
ANTIOXIDANTS
GLAUCOMA ASSOCIATED WITH EXFOLIATION SYNDROME
PRIMARY OPEN ANGLE GLAUCOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv PURPOSE: To establish the antioxidant status of the aqueous humour in glaucoma associated with exfoliation syndrome (XFG) and to compare it to primary open-angle glaucoma (POAG) and cataract patients. METHODS: Patients were diagnosed with POAG, XFG, or cataract (n=25 for each group). Total reactive antioxidant potential (TRAP) was measured by chemiluminescence. Ascorbic acid levels and the activities of catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD) were measured spectrophotometrically.ResultsTRAP value was lower in XFG (28+/-2 microM Trolox) than in POAG (55+/-8 microM Trolox; P<0.001). TRAP values in both glaucomas were lower than the cataract value (124+/-5 microM Trolox; P<0.001). A decrease in ascorbic acid was measured in XFG (230+/-20 microM) compared with POAG (415+/-17 microM; P<0.001). Ascorbic acid in both glaucomas was lower than in cataract (720+/-30 microM; P<0.001). A significant increase in GPx was found in XFG (30+/-2 U/ml) compared with POAG (16+/-3 U/ml). GPx activity in both glaucomas was increased when compared with cataracts (6+/-2 U/ml; P<0.001). A significant increase of 67% in SOD activity was observed in the glaucoma group vscataract group (27+/-3 U/ml; P<0.001), but no changes were found between both glaucomas. CONCLUSIONS: The antioxidant status of the aqueous humour may play a role in the pathophysiology of both glaucomas.
Fil: Ferreira, Sandra María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Lerner, S.F. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Brunzini, R. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Evelson, Pablo Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Llesuy, Susana Francisca. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
description PURPOSE: To establish the antioxidant status of the aqueous humour in glaucoma associated with exfoliation syndrome (XFG) and to compare it to primary open-angle glaucoma (POAG) and cataract patients. METHODS: Patients were diagnosed with POAG, XFG, or cataract (n=25 for each group). Total reactive antioxidant potential (TRAP) was measured by chemiluminescence. Ascorbic acid levels and the activities of catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD) were measured spectrophotometrically.ResultsTRAP value was lower in XFG (28+/-2 microM Trolox) than in POAG (55+/-8 microM Trolox; P<0.001). TRAP values in both glaucomas were lower than the cataract value (124+/-5 microM Trolox; P<0.001). A decrease in ascorbic acid was measured in XFG (230+/-20 microM) compared with POAG (415+/-17 microM; P<0.001). Ascorbic acid in both glaucomas was lower than in cataract (720+/-30 microM; P<0.001). A significant increase in GPx was found in XFG (30+/-2 U/ml) compared with POAG (16+/-3 U/ml). GPx activity in both glaucomas was increased when compared with cataracts (6+/-2 U/ml; P<0.001). A significant increase of 67% in SOD activity was observed in the glaucoma group vscataract group (27+/-3 U/ml; P<0.001), but no changes were found between both glaucomas. CONCLUSIONS: The antioxidant status of the aqueous humour may play a role in the pathophysiology of both glaucomas.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102636
Ferreira, Sandra María; Lerner, S.F; Brunzini, R; Evelson, Pablo Andrés; Llesuy, Susana Francisca; Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome; Nature Publishing Group; Eye; 23; 12-2009; 1691-1697
0950-222X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102636
identifier_str_mv Ferreira, Sandra María; Lerner, S.F; Brunzini, R; Evelson, Pablo Andrés; Llesuy, Susana Francisca; Antioxidant status in the aqueous humour of patients with glaucoma associated with exfoliation syndrome; Nature Publishing Group; Eye; 23; 12-2009; 1691-1697
0950-222X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/eye2008352?draft=marketing
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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