Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors

Autores
Perullini, Ana Mercedes; Amoura, Makhlouf; Roux, Cécile; Coradin, Thibaud; Livage, Jacques; Japas, María Laura; Jobbagy, Matias; Aldabe, Sara Alfonsina
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of sol–gel based processes for the encapsulation of living cells is a challenging task where the host material must be optimized both in terms of intrinsic properties and impact on cell viability. Here, the mechanical stability of silica hosts obtained via a mixed aqueous route based on sodium silicate–silica nanoparticle mixtures could be improved by increasing the relative content of molecular precursors over colloidal silica. The specific surface area, pore volume and diffusion properties of the host were also modified. Moreover, this resulted in an increase in the ionic strength, inducing high osmolarity that is detrimental to encapsulated Escherichia coli bacteria. Glycine betaine, a well-known E. coli osmoprotector, could be successfully used as an additive to the sol–gel formulation to limit this osmotic stress. Compared to the previously studied glycerol additive, glycine betaine was found more efficient to preserve the bacterial viability. Moreover, this efficiency was obtained with much lower amounts of glycine betaine, whose addition has therefore no detrimental impact on the silica host structure. These data provide new information about the different cellular stresses resulting from the sol–gel encapsulation process and demonstrate the importance of combining chemical and biological approaches to design more robust functional ‘‘living’’ materials.
Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Amoura, Makhlouf. Centre National de la Recherche Scientifique; Francia
Fil: Roux, Cécile. Centre National de la Recherche Scientifique; Francia
Fil: Coradin, Thibaud. Centre National de la Recherche Scientifique; Francia
Fil: Livage, Jacques. Centre National de la Recherche Scientifique; Francia
Fil: Japas, María Laura. Comisión Nacional de Energía Atómica. Gerencia del Área de Seguridad Nuclear y Ambiente. Gerencia de Química (CAC); Argentina
Fil: Jobbagy, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Aldabe, Sara Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Materia
hydrogels
encapsulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/279159
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/279159
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectorsPerullini, Ana MercedesAmoura, MakhloufRoux, CécileCoradin, ThibaudLivage, JacquesJapas, María LauraJobbagy, MatiasAldabe, Sara Alfonsinahydrogelsencapsulationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The development of sol–gel based processes for the encapsulation of living cells is a challenging task where the host material must be optimized both in terms of intrinsic properties and impact on cell viability. Here, the mechanical stability of silica hosts obtained via a mixed aqueous route based on sodium silicate–silica nanoparticle mixtures could be improved by increasing the relative content of molecular precursors over colloidal silica. The specific surface area, pore volume and diffusion properties of the host were also modified. Moreover, this resulted in an increase in the ionic strength, inducing high osmolarity that is detrimental to encapsulated Escherichia coli bacteria. Glycine betaine, a well-known E. coli osmoprotector, could be successfully used as an additive to the sol–gel formulation to limit this osmotic stress. Compared to the previously studied glycerol additive, glycine betaine was found more efficient to preserve the bacterial viability. Moreover, this efficiency was obtained with much lower amounts of glycine betaine, whose addition has therefore no detrimental impact on the silica host structure. These data provide new information about the different cellular stresses resulting from the sol–gel encapsulation process and demonstrate the importance of combining chemical and biological approaches to design more robust functional ‘‘living’’ materials.Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Amoura, Makhlouf. Centre National de la Recherche Scientifique; FranciaFil: Roux, Cécile. Centre National de la Recherche Scientifique; FranciaFil: Coradin, Thibaud. Centre National de la Recherche Scientifique; FranciaFil: Livage, Jacques. Centre National de la Recherche Scientifique; FranciaFil: Japas, María Laura. Comisión Nacional de Energía Atómica. Gerencia del Área de Seguridad Nuclear y Ambiente. Gerencia de Química (CAC); ArgentinaFil: Jobbagy, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Aldabe, Sara Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaRoyal Society of Chemistry2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/279159Perullini, Ana Mercedes; Amoura, Makhlouf; Roux, Cécile; Coradin, Thibaud; Livage, Jacques; et al.; Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors; Royal Society of Chemistry; Journal Of Materials Chemistry; 21; 2-2011; 4546-45520959-9428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2011/jm/c0jm03948ainfo:eu-repo/semantics/altIdentifier/doi/10.1039/C0JM03948Ainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T12:19:59Zoai:ri.conicet.gov.ar:11336/279159instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 12:19:59.448CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors
title Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors
spellingShingle Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors
Perullini, Ana Mercedes
hydrogels
encapsulation
title_short Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors
title_full Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors
title_fullStr Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors
title_full_unstemmed Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors
title_sort Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors
dc.creator.none.fl_str_mv Perullini, Ana Mercedes
Amoura, Makhlouf
Roux, Cécile
Coradin, Thibaud
Livage, Jacques
Japas, María Laura
Jobbagy, Matias
Aldabe, Sara Alfonsina
author Perullini, Ana Mercedes
author_facet Perullini, Ana Mercedes
Amoura, Makhlouf
Roux, Cécile
Coradin, Thibaud
Livage, Jacques
Japas, María Laura
Jobbagy, Matias
Aldabe, Sara Alfonsina
author_role author
author2 Amoura, Makhlouf
Roux, Cécile
Coradin, Thibaud
Livage, Jacques
Japas, María Laura
Jobbagy, Matias
Aldabe, Sara Alfonsina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv hydrogels
encapsulation
topic hydrogels
encapsulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The development of sol–gel based processes for the encapsulation of living cells is a challenging task where the host material must be optimized both in terms of intrinsic properties and impact on cell viability. Here, the mechanical stability of silica hosts obtained via a mixed aqueous route based on sodium silicate–silica nanoparticle mixtures could be improved by increasing the relative content of molecular precursors over colloidal silica. The specific surface area, pore volume and diffusion properties of the host were also modified. Moreover, this resulted in an increase in the ionic strength, inducing high osmolarity that is detrimental to encapsulated Escherichia coli bacteria. Glycine betaine, a well-known E. coli osmoprotector, could be successfully used as an additive to the sol–gel formulation to limit this osmotic stress. Compared to the previously studied glycerol additive, glycine betaine was found more efficient to preserve the bacterial viability. Moreover, this efficiency was obtained with much lower amounts of glycine betaine, whose addition has therefore no detrimental impact on the silica host structure. These data provide new information about the different cellular stresses resulting from the sol–gel encapsulation process and demonstrate the importance of combining chemical and biological approaches to design more robust functional ‘‘living’’ materials.
Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Amoura, Makhlouf. Centre National de la Recherche Scientifique; Francia
Fil: Roux, Cécile. Centre National de la Recherche Scientifique; Francia
Fil: Coradin, Thibaud. Centre National de la Recherche Scientifique; Francia
Fil: Livage, Jacques. Centre National de la Recherche Scientifique; Francia
Fil: Japas, María Laura. Comisión Nacional de Energía Atómica. Gerencia del Área de Seguridad Nuclear y Ambiente. Gerencia de Química (CAC); Argentina
Fil: Jobbagy, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Aldabe, Sara Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
description The development of sol–gel based processes for the encapsulation of living cells is a challenging task where the host material must be optimized both in terms of intrinsic properties and impact on cell viability. Here, the mechanical stability of silica hosts obtained via a mixed aqueous route based on sodium silicate–silica nanoparticle mixtures could be improved by increasing the relative content of molecular precursors over colloidal silica. The specific surface area, pore volume and diffusion properties of the host were also modified. Moreover, this resulted in an increase in the ionic strength, inducing high osmolarity that is detrimental to encapsulated Escherichia coli bacteria. Glycine betaine, a well-known E. coli osmoprotector, could be successfully used as an additive to the sol–gel formulation to limit this osmotic stress. Compared to the previously studied glycerol additive, glycine betaine was found more efficient to preserve the bacterial viability. Moreover, this efficiency was obtained with much lower amounts of glycine betaine, whose addition has therefore no detrimental impact on the silica host structure. These data provide new information about the different cellular stresses resulting from the sol–gel encapsulation process and demonstrate the importance of combining chemical and biological approaches to design more robust functional ‘‘living’’ materials.
publishDate 2011
dc.date.none.fl_str_mv 2011-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/279159
Perullini, Ana Mercedes; Amoura, Makhlouf; Roux, Cécile; Coradin, Thibaud; Livage, Jacques; et al.; Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors; Royal Society of Chemistry; Journal Of Materials Chemistry; 21; 2-2011; 4546-4552
0959-9428
CONICET Digital
CONICET
url http://hdl.handle.net/11336/279159
identifier_str_mv Perullini, Ana Mercedes; Amoura, Makhlouf; Roux, Cécile; Coradin, Thibaud; Livage, Jacques; et al.; Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors; Royal Society of Chemistry; Journal Of Materials Chemistry; 21; 2-2011; 4546-4552
0959-9428
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2011/jm/c0jm03948a
info:eu-repo/semantics/altIdentifier/doi/10.1039/C0JM03948A
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.065482

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