Low-cost equipment for electroformation of Giant Unilamellar Vesicles

Autores
Bellon, Javier Alejandro; Pino, Marcelo Javier; Wilke, Natalia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Giant Unilamellar Vesicles (GUVs) constitute a very useful model for biomembranes. They are usually generated by electroformation using sinusoidal potential waves applied between two platinum electrodes or electrodes composed of glasses covered by indium tin oxide (ITO). Here we present the design of a programmable, compact and low cost wave generator that allows applying different waveforms, even with initial non-periodical shapes. Thus, it allows to optimize the potential waveform for the particular GUV’ composition. We also describe the design of a low cost, reusable chamber with stainless steel electrodes that allows using very small volumes of solution (0.3 mL). With this chamber, a larger number of GUVs were generated in less electroformation times compared to ITO electrodes. The general characteristics of the obtained GUVs are comparable to those electroformed in platinum or ITO surfaces. The chamber, combined with the wave generator comprises a simple, reusable, programmable and low cost system for the formation of GUVs.
Fil: Bellon, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Pino, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Materia
GUV'S GENERATION
ITO
LIPID MEMBRANES
PLATINUM
STAINLESS STEEL
WAVE GENERATOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95817
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Low-cost equipment for electroformation of Giant Unilamellar VesiclesBellon, Javier AlejandroPino, Marcelo JavierWilke, NataliaGUV'S GENERATIONITOLIPID MEMBRANESPLATINUMSTAINLESS STEELWAVE GENERATORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Giant Unilamellar Vesicles (GUVs) constitute a very useful model for biomembranes. They are usually generated by electroformation using sinusoidal potential waves applied between two platinum electrodes or electrodes composed of glasses covered by indium tin oxide (ITO). Here we present the design of a programmable, compact and low cost wave generator that allows applying different waveforms, even with initial non-periodical shapes. Thus, it allows to optimize the potential waveform for the particular GUV’ composition. We also describe the design of a low cost, reusable chamber with stainless steel electrodes that allows using very small volumes of solution (0.3 mL). With this chamber, a larger number of GUVs were generated in less electroformation times compared to ITO electrodes. The general characteristics of the obtained GUVs are comparable to those electroformed in platinum or ITO surfaces. The chamber, combined with the wave generator comprises a simple, reusable, programmable and low cost system for the formation of GUVs.Fil: Bellon, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Pino, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaElsevier2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95817Bellon, Javier Alejandro; Pino, Marcelo Javier; Wilke, Natalia; Low-cost equipment for electroformation of Giant Unilamellar Vesicles; Elsevier; HardwareX; 4; 10-2018; 1-112468-0672CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2468067218300440info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ohx.2018.e00037info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:06Zoai:ri.conicet.gov.ar:11336/95817instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:07.24CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Low-cost equipment for electroformation of Giant Unilamellar Vesicles
title Low-cost equipment for electroformation of Giant Unilamellar Vesicles
spellingShingle Low-cost equipment for electroformation of Giant Unilamellar Vesicles
Bellon, Javier Alejandro
GUV'S GENERATION
ITO
LIPID MEMBRANES
PLATINUM
STAINLESS STEEL
WAVE GENERATOR
title_short Low-cost equipment for electroformation of Giant Unilamellar Vesicles
title_full Low-cost equipment for electroformation of Giant Unilamellar Vesicles
title_fullStr Low-cost equipment for electroformation of Giant Unilamellar Vesicles
title_full_unstemmed Low-cost equipment for electroformation of Giant Unilamellar Vesicles
title_sort Low-cost equipment for electroformation of Giant Unilamellar Vesicles
dc.creator.none.fl_str_mv Bellon, Javier Alejandro
Pino, Marcelo Javier
Wilke, Natalia
author Bellon, Javier Alejandro
author_facet Bellon, Javier Alejandro
Pino, Marcelo Javier
Wilke, Natalia
author_role author
author2 Pino, Marcelo Javier
Wilke, Natalia
author2_role author
author
dc.subject.none.fl_str_mv GUV'S GENERATION
ITO
LIPID MEMBRANES
PLATINUM
STAINLESS STEEL
WAVE GENERATOR
topic GUV'S GENERATION
ITO
LIPID MEMBRANES
PLATINUM
STAINLESS STEEL
WAVE GENERATOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Giant Unilamellar Vesicles (GUVs) constitute a very useful model for biomembranes. They are usually generated by electroformation using sinusoidal potential waves applied between two platinum electrodes or electrodes composed of glasses covered by indium tin oxide (ITO). Here we present the design of a programmable, compact and low cost wave generator that allows applying different waveforms, even with initial non-periodical shapes. Thus, it allows to optimize the potential waveform for the particular GUV’ composition. We also describe the design of a low cost, reusable chamber with stainless steel electrodes that allows using very small volumes of solution (0.3 mL). With this chamber, a larger number of GUVs were generated in less electroformation times compared to ITO electrodes. The general characteristics of the obtained GUVs are comparable to those electroformed in platinum or ITO surfaces. The chamber, combined with the wave generator comprises a simple, reusable, programmable and low cost system for the formation of GUVs.
Fil: Bellon, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Pino, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
description Giant Unilamellar Vesicles (GUVs) constitute a very useful model for biomembranes. They are usually generated by electroformation using sinusoidal potential waves applied between two platinum electrodes or electrodes composed of glasses covered by indium tin oxide (ITO). Here we present the design of a programmable, compact and low cost wave generator that allows applying different waveforms, even with initial non-periodical shapes. Thus, it allows to optimize the potential waveform for the particular GUV’ composition. We also describe the design of a low cost, reusable chamber with stainless steel electrodes that allows using very small volumes of solution (0.3 mL). With this chamber, a larger number of GUVs were generated in less electroformation times compared to ITO electrodes. The general characteristics of the obtained GUVs are comparable to those electroformed in platinum or ITO surfaces. The chamber, combined with the wave generator comprises a simple, reusable, programmable and low cost system for the formation of GUVs.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95817
Bellon, Javier Alejandro; Pino, Marcelo Javier; Wilke, Natalia; Low-cost equipment for electroformation of Giant Unilamellar Vesicles; Elsevier; HardwareX; 4; 10-2018; 1-11
2468-0672
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95817
identifier_str_mv Bellon, Javier Alejandro; Pino, Marcelo Javier; Wilke, Natalia; Low-cost equipment for electroformation of Giant Unilamellar Vesicles; Elsevier; HardwareX; 4; 10-2018; 1-11
2468-0672
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2468067218300440
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ohx.2018.e00037
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613091647553536
score 13.070432

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