Real-time NMR monitoring of biological activities in complex physiological environments

Autores
Smith, Matthew J.; Marshall, Christopher B.; Theillet, Francois Xavier; Binolfi, Andrés; Selenko, Philipp; Ikura, Mitsuhiko
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of 'native' biological activities, we require quantitative tools for time-resolved in situ analyses in physiologically relevant settings. Here, we outline the use of high-resolution NMR spectroscopy to directly observe biological reactions in complex environments and in real-time. Specifically, we discuss how real-time NMR (RT-NMR) methods have delineated insights into metabolic processes, post-translational protein modifications, activities of cellular GTPases and their regulators, as well as of protein folding events.
Fil: Smith, Matthew J.. Ontario Cancer Institute; Canadá
Fil: Marshall, Christopher B.. Ontario Cancer Institute; Canadá
Fil: Theillet, Francois Xavier. Leibniz Institute of Molecular Pharmacology; Alemania
Fil: Binolfi, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Leibniz Institute of Molecular Pharmacology; Alemania
Fil: Selenko, Philipp. Leibniz Institute of Molecular Pharmacology; Alemania
Fil: Ikura, Mitsuhiko. Ontario Cancer Institute; Canadá. University of Toronto; Canadá
Materia
Nmr
Post-Translational Modifications
Kinetics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/51058
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/51058
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Real-time NMR monitoring of biological activities in complex physiological environmentsSmith, Matthew J.Marshall, Christopher B.Theillet, Francois XavierBinolfi, AndrésSelenko, PhilippIkura, MitsuhikoNmrPost-Translational ModificationsKineticshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of 'native' biological activities, we require quantitative tools for time-resolved in situ analyses in physiologically relevant settings. Here, we outline the use of high-resolution NMR spectroscopy to directly observe biological reactions in complex environments and in real-time. Specifically, we discuss how real-time NMR (RT-NMR) methods have delineated insights into metabolic processes, post-translational protein modifications, activities of cellular GTPases and their regulators, as well as of protein folding events.Fil: Smith, Matthew J.. Ontario Cancer Institute; CanadáFil: Marshall, Christopher B.. Ontario Cancer Institute; CanadáFil: Theillet, Francois Xavier. Leibniz Institute of Molecular Pharmacology; AlemaniaFil: Binolfi, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Leibniz Institute of Molecular Pharmacology; AlemaniaFil: Selenko, Philipp. Leibniz Institute of Molecular Pharmacology; AlemaniaFil: Ikura, Mitsuhiko. Ontario Cancer Institute; Canadá. University of Toronto; CanadáCurrent Biology2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/51058Smith, Matthew J.; Marshall, Christopher B.; Theillet, Francois Xavier; Binolfi, Andrés; Selenko, Philipp; et al.; Real-time NMR monitoring of biological activities in complex physiological environments; Current Biology; Current Opinion In Structural Biology; 32; 6-2015; 39-470959-440XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.sbi.2015.02.003info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0959440X15000123info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:28Zoai:ri.conicet.gov.ar:11336/51058instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:29.185CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Real-time NMR monitoring of biological activities in complex physiological environments
title Real-time NMR monitoring of biological activities in complex physiological environments
spellingShingle Real-time NMR monitoring of biological activities in complex physiological environments
Smith, Matthew J.
Nmr
Post-Translational Modifications
Kinetics
title_short Real-time NMR monitoring of biological activities in complex physiological environments
title_full Real-time NMR monitoring of biological activities in complex physiological environments
title_fullStr Real-time NMR monitoring of biological activities in complex physiological environments
title_full_unstemmed Real-time NMR monitoring of biological activities in complex physiological environments
title_sort Real-time NMR monitoring of biological activities in complex physiological environments
dc.creator.none.fl_str_mv Smith, Matthew J.
Marshall, Christopher B.
Theillet, Francois Xavier
Binolfi, Andrés
Selenko, Philipp
Ikura, Mitsuhiko
author Smith, Matthew J.
author_facet Smith, Matthew J.
Marshall, Christopher B.
Theillet, Francois Xavier
Binolfi, Andrés
Selenko, Philipp
Ikura, Mitsuhiko
author_role author
author2 Marshall, Christopher B.
Theillet, Francois Xavier
Binolfi, Andrés
Selenko, Philipp
Ikura, Mitsuhiko
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Nmr
Post-Translational Modifications
Kinetics
topic Nmr
Post-Translational Modifications
Kinetics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of 'native' biological activities, we require quantitative tools for time-resolved in situ analyses in physiologically relevant settings. Here, we outline the use of high-resolution NMR spectroscopy to directly observe biological reactions in complex environments and in real-time. Specifically, we discuss how real-time NMR (RT-NMR) methods have delineated insights into metabolic processes, post-translational protein modifications, activities of cellular GTPases and their regulators, as well as of protein folding events.
Fil: Smith, Matthew J.. Ontario Cancer Institute; Canadá
Fil: Marshall, Christopher B.. Ontario Cancer Institute; Canadá
Fil: Theillet, Francois Xavier. Leibniz Institute of Molecular Pharmacology; Alemania
Fil: Binolfi, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Leibniz Institute of Molecular Pharmacology; Alemania
Fil: Selenko, Philipp. Leibniz Institute of Molecular Pharmacology; Alemania
Fil: Ikura, Mitsuhiko. Ontario Cancer Institute; Canadá. University of Toronto; Canadá
description Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of 'native' biological activities, we require quantitative tools for time-resolved in situ analyses in physiologically relevant settings. Here, we outline the use of high-resolution NMR spectroscopy to directly observe biological reactions in complex environments and in real-time. Specifically, we discuss how real-time NMR (RT-NMR) methods have delineated insights into metabolic processes, post-translational protein modifications, activities of cellular GTPases and their regulators, as well as of protein folding events.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/51058
Smith, Matthew J.; Marshall, Christopher B.; Theillet, Francois Xavier; Binolfi, Andrés; Selenko, Philipp; et al.; Real-time NMR monitoring of biological activities in complex physiological environments; Current Biology; Current Opinion In Structural Biology; 32; 6-2015; 39-47
0959-440X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/51058
identifier_str_mv Smith, Matthew J.; Marshall, Christopher B.; Theillet, Francois Xavier; Binolfi, Andrés; Selenko, Philipp; et al.; Real-time NMR monitoring of biological activities in complex physiological environments; Current Biology; Current Opinion In Structural Biology; 32; 6-2015; 39-47
0959-440X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.sbi.2015.02.003
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0959440X15000123
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Current Biology
publisher.none.fl_str_mv Current Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268604926525440
score 13.13397

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