A new ELISA for determination of potency in snake antivenoms
- Autores
- Rial, A.; Morais, V.; Rossi, S.; Massaldi, Hugo Alberto
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Abstract A competitive ELISA for potency determination of bothropic equine antivenom was developed and compared to the conventional in vivo ED50 assay, with the aim of partially substituting the in vivo assay in the monitoring of antivenom immunoglobulin levels. On this purpose, blood samples were taken at different times during and after the immunization protocol of the lot of horses used for production of snake antivenom at the Instituto de Higiene, Uruguay. Both the competitive ELISA and the ED50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.in vivo ED50 assay, with the aim of partially substituting the in vivo assay in the monitoring of antivenom immunoglobulin levels. On this purpose, blood samples were taken at different times during and after the immunization protocol of the lot of horses used for production of snake antivenom at the Instituto de Higiene, Uruguay. Both the competitive ELISA and the ED50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.0)2 fragment.
Fil: Rial, A.. Universidad de la República; Uruguay
Fil: Morais, V.. Universidad de la República; Uruguay
Fil: Rossi, S.. Universidad de la República; Uruguay
Fil: Massaldi, Hugo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de la República; Uruguay - Materia
-
ANTIVENOM
ELISA
ED50 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/248323
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A new ELISA for determination of potency in snake antivenomsRial, A.Morais, V.Rossi, S.Massaldi, Hugo AlbertoANTIVENOMELISAED50https://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Abstract A competitive ELISA for potency determination of bothropic equine antivenom was developed and compared to the conventional in vivo ED50 assay, with the aim of partially substituting the in vivo assay in the monitoring of antivenom immunoglobulin levels. On this purpose, blood samples were taken at different times during and after the immunization protocol of the lot of horses used for production of snake antivenom at the Instituto de Higiene, Uruguay. Both the competitive ELISA and the ED50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.in vivo ED50 assay, with the aim of partially substituting the in vivo assay in the monitoring of antivenom immunoglobulin levels. On this purpose, blood samples were taken at different times during and after the immunization protocol of the lot of horses used for production of snake antivenom at the Instituto de Higiene, Uruguay. Both the competitive ELISA and the ED50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.0)2 fragment.Fil: Rial, A.. Universidad de la República; UruguayFil: Morais, V.. Universidad de la República; UruguayFil: Rossi, S.. Universidad de la República; UruguayFil: Massaldi, Hugo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de la República; UruguayPergamon-Elsevier Science Ltd2006-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/248323Rial, A.; Morais, V.; Rossi, S.; Massaldi, Hugo Alberto; A new ELISA for determination of potency in snake antivenoms; Pergamon-Elsevier Science Ltd; Toxicon; 48; 4; 9-2006; 462-4660041-0101CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0041010106002352info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2006.07.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:29Zoai:ri.conicet.gov.ar:11336/248323instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:29.714CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A new ELISA for determination of potency in snake antivenoms |
title |
A new ELISA for determination of potency in snake antivenoms |
spellingShingle |
A new ELISA for determination of potency in snake antivenoms Rial, A. ANTIVENOM ELISA ED50 |
title_short |
A new ELISA for determination of potency in snake antivenoms |
title_full |
A new ELISA for determination of potency in snake antivenoms |
title_fullStr |
A new ELISA for determination of potency in snake antivenoms |
title_full_unstemmed |
A new ELISA for determination of potency in snake antivenoms |
title_sort |
A new ELISA for determination of potency in snake antivenoms |
dc.creator.none.fl_str_mv |
Rial, A. Morais, V. Rossi, S. Massaldi, Hugo Alberto |
author |
Rial, A. |
author_facet |
Rial, A. Morais, V. Rossi, S. Massaldi, Hugo Alberto |
author_role |
author |
author2 |
Morais, V. Rossi, S. Massaldi, Hugo Alberto |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
ANTIVENOM ELISA ED50 |
topic |
ANTIVENOM ELISA ED50 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Abstract A competitive ELISA for potency determination of bothropic equine antivenom was developed and compared to the conventional in vivo ED50 assay, with the aim of partially substituting the in vivo assay in the monitoring of antivenom immunoglobulin levels. On this purpose, blood samples were taken at different times during and after the immunization protocol of the lot of horses used for production of snake antivenom at the Instituto de Higiene, Uruguay. Both the competitive ELISA and the ED50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.in vivo ED50 assay, with the aim of partially substituting the in vivo assay in the monitoring of antivenom immunoglobulin levels. On this purpose, blood samples were taken at different times during and after the immunization protocol of the lot of horses used for production of snake antivenom at the Instituto de Higiene, Uruguay. Both the competitive ELISA and the ED50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.0)2 fragment. Fil: Rial, A.. Universidad de la República; Uruguay Fil: Morais, V.. Universidad de la República; Uruguay Fil: Rossi, S.. Universidad de la República; Uruguay Fil: Massaldi, Hugo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de la República; Uruguay |
description |
Abstract A competitive ELISA for potency determination of bothropic equine antivenom was developed and compared to the conventional in vivo ED50 assay, with the aim of partially substituting the in vivo assay in the monitoring of antivenom immunoglobulin levels. On this purpose, blood samples were taken at different times during and after the immunization protocol of the lot of horses used for production of snake antivenom at the Instituto de Higiene, Uruguay. Both the competitive ELISA and the ED50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.in vivo ED50 assay, with the aim of partially substituting the in vivo assay in the monitoring of antivenom immunoglobulin levels. On this purpose, blood samples were taken at different times during and after the immunization protocol of the lot of horses used for production of snake antivenom at the Instituto de Higiene, Uruguay. Both the competitive ELISA and the ED50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.50 assay were performed on those samples. In addition, a group of five commercial pepsindigested antivenoms were tested by both methods. A significant (Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.Po0.001) correlation (Pearson’s r ¼ 0.957) was found between the ELISA titres and the corresponding ED50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.50 values, indicating that the in vitro test can estimate the neutralizing antibody capacity of the sera as well as the in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.in vivo assay. By means of this new ELISA, it was found that the immunized animals maintained good venom antibody titres, in the order of 20–50% of the maximum achieved, even 10 month after the end of the immunization schedule. The main advantage of our ELISA design is its ability to correctly estimate the neutralization capacity of crude hyperimmune plasma and antivenom sera independently of their antibody composition in terms of whole IgG or F(ab0)2 fragment.0)2 fragment. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/248323 Rial, A.; Morais, V.; Rossi, S.; Massaldi, Hugo Alberto; A new ELISA for determination of potency in snake antivenoms; Pergamon-Elsevier Science Ltd; Toxicon; 48; 4; 9-2006; 462-466 0041-0101 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/248323 |
identifier_str_mv |
Rial, A.; Morais, V.; Rossi, S.; Massaldi, Hugo Alberto; A new ELISA for determination of potency in snake antivenoms; Pergamon-Elsevier Science Ltd; Toxicon; 48; 4; 9-2006; 462-466 0041-0101 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0041010106002352 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2006.07.004 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |