Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)

Autores
Siroski, Pablo Ariel; Merchant, Mark; Parachu Marco, Maria Virginia; Piña, Carlos Ignacio; Ortega, Hugo Hector
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Characterization of the serum complement activity of the broad-snouted caiman Caiman latirostris (Crocodilia: Alligatoridae). Zoological Studies 49(1): 64-70. The sheep red blood cell (SRBC) hemolysis assay was used to detect and characterize complement-system activity of broad-snouted caiman (Caiman latirostris) serum. The hemolytic activity of caiman serum was inhibited by 2 classic inhibitors (EDTA and heat) suggesting the existence of complement-system activity. In addition, we found that the capacity of C. latirostris serum to disrupt SRBCs was concentration, temperature, and kinetics dependent. Hemolytic activity was detected from a very low concentration (< 10%) of caiman serum and increased until 100%. Temperature influenced the activity of the serum by disrupting SRBC membranes. The serum showed a peak of hemolysis between 30 and 40°C, within which lies the optimal temperature caimans prefer during thermoregulation for normal physiological processes. Hemolytic activity rapidly occurred at 2 min, and maximum activity was detected at 60 min. These observations reflect previously reported findings in other crocodilian species (Alligator mississippiensis, Crocodylus johnstoni, and Cro. porosus), thus adding to the knowledge of the role of the complement system in immunological activities of crocodilians.
Fil: Siroski, Pablo Ariel. Provincia de Santa Fe. Secretaría de Medio Ambiente; Argentina. Proyecto Yacaré; Argentina
Fil: Merchant, Mark. McNeese State University; Estados Unidos
Fil: Parachu Marco, Maria Virginia. Proyecto Yacaré; Argentina. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Fil: Piña, Carlos Ignacio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Proyecto Yacaré; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Materia
CROCODILIANS
ECTOTHERMICS
HEMOLYTIC ASSAY
IMMUNOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53525

id CONICETDig_3e525cc9aa6129a18d9a42c4403b79a6
oai_identifier_str oai:ri.conicet.gov.ar:11336/53525
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)Siroski, Pablo ArielMerchant, MarkParachu Marco, Maria VirginiaPiña, Carlos IgnacioOrtega, Hugo HectorCROCODILIANSECTOTHERMICSHEMOLYTIC ASSAYIMMUNOLOGYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Characterization of the serum complement activity of the broad-snouted caiman Caiman latirostris (Crocodilia: Alligatoridae). Zoological Studies 49(1): 64-70. The sheep red blood cell (SRBC) hemolysis assay was used to detect and characterize complement-system activity of broad-snouted caiman (Caiman latirostris) serum. The hemolytic activity of caiman serum was inhibited by 2 classic inhibitors (EDTA and heat) suggesting the existence of complement-system activity. In addition, we found that the capacity of C. latirostris serum to disrupt SRBCs was concentration, temperature, and kinetics dependent. Hemolytic activity was detected from a very low concentration (< 10%) of caiman serum and increased until 100%. Temperature influenced the activity of the serum by disrupting SRBC membranes. The serum showed a peak of hemolysis between 30 and 40°C, within which lies the optimal temperature caimans prefer during thermoregulation for normal physiological processes. Hemolytic activity rapidly occurred at 2 min, and maximum activity was detected at 60 min. These observations reflect previously reported findings in other crocodilian species (Alligator mississippiensis, Crocodylus johnstoni, and Cro. porosus), thus adding to the knowledge of the role of the complement system in immunological activities of crocodilians.Fil: Siroski, Pablo Ariel. Provincia de Santa Fe. Secretaría de Medio Ambiente; Argentina. Proyecto Yacaré; ArgentinaFil: Merchant, Mark. McNeese State University; Estados UnidosFil: Parachu Marco, Maria Virginia. Proyecto Yacaré; Argentina. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Piña, Carlos Ignacio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Proyecto Yacaré; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaAcademia Sinica2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53525Siroski, Pablo Ariel; Merchant, Mark; Parachu Marco, Maria Virginia; Piña, Carlos Ignacio; Ortega, Hugo Hector; Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae); Academia Sinica; Zoological Studies; 49; 1; 1-2010; 64-701021-5506CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://zoolstud.sinica.edu.tw/Journals/49.1/64.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:20Zoai:ri.conicet.gov.ar:11336/53525instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:21.151CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)
title Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)
spellingShingle Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)
Siroski, Pablo Ariel
CROCODILIANS
ECTOTHERMICS
HEMOLYTIC ASSAY
IMMUNOLOGY
title_short Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)
title_full Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)
title_fullStr Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)
title_full_unstemmed Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)
title_sort Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)
dc.creator.none.fl_str_mv Siroski, Pablo Ariel
Merchant, Mark
Parachu Marco, Maria Virginia
Piña, Carlos Ignacio
Ortega, Hugo Hector
author Siroski, Pablo Ariel
author_facet Siroski, Pablo Ariel
Merchant, Mark
Parachu Marco, Maria Virginia
Piña, Carlos Ignacio
Ortega, Hugo Hector
author_role author
author2 Merchant, Mark
Parachu Marco, Maria Virginia
Piña, Carlos Ignacio
Ortega, Hugo Hector
author2_role author
author
author
author
dc.subject.none.fl_str_mv CROCODILIANS
ECTOTHERMICS
HEMOLYTIC ASSAY
IMMUNOLOGY
topic CROCODILIANS
ECTOTHERMICS
HEMOLYTIC ASSAY
IMMUNOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Characterization of the serum complement activity of the broad-snouted caiman Caiman latirostris (Crocodilia: Alligatoridae). Zoological Studies 49(1): 64-70. The sheep red blood cell (SRBC) hemolysis assay was used to detect and characterize complement-system activity of broad-snouted caiman (Caiman latirostris) serum. The hemolytic activity of caiman serum was inhibited by 2 classic inhibitors (EDTA and heat) suggesting the existence of complement-system activity. In addition, we found that the capacity of C. latirostris serum to disrupt SRBCs was concentration, temperature, and kinetics dependent. Hemolytic activity was detected from a very low concentration (< 10%) of caiman serum and increased until 100%. Temperature influenced the activity of the serum by disrupting SRBC membranes. The serum showed a peak of hemolysis between 30 and 40°C, within which lies the optimal temperature caimans prefer during thermoregulation for normal physiological processes. Hemolytic activity rapidly occurred at 2 min, and maximum activity was detected at 60 min. These observations reflect previously reported findings in other crocodilian species (Alligator mississippiensis, Crocodylus johnstoni, and Cro. porosus), thus adding to the knowledge of the role of the complement system in immunological activities of crocodilians.
Fil: Siroski, Pablo Ariel. Provincia de Santa Fe. Secretaría de Medio Ambiente; Argentina. Proyecto Yacaré; Argentina
Fil: Merchant, Mark. McNeese State University; Estados Unidos
Fil: Parachu Marco, Maria Virginia. Proyecto Yacaré; Argentina. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Fil: Piña, Carlos Ignacio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Proyecto Yacaré; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
description Characterization of the serum complement activity of the broad-snouted caiman Caiman latirostris (Crocodilia: Alligatoridae). Zoological Studies 49(1): 64-70. The sheep red blood cell (SRBC) hemolysis assay was used to detect and characterize complement-system activity of broad-snouted caiman (Caiman latirostris) serum. The hemolytic activity of caiman serum was inhibited by 2 classic inhibitors (EDTA and heat) suggesting the existence of complement-system activity. In addition, we found that the capacity of C. latirostris serum to disrupt SRBCs was concentration, temperature, and kinetics dependent. Hemolytic activity was detected from a very low concentration (< 10%) of caiman serum and increased until 100%. Temperature influenced the activity of the serum by disrupting SRBC membranes. The serum showed a peak of hemolysis between 30 and 40°C, within which lies the optimal temperature caimans prefer during thermoregulation for normal physiological processes. Hemolytic activity rapidly occurred at 2 min, and maximum activity was detected at 60 min. These observations reflect previously reported findings in other crocodilian species (Alligator mississippiensis, Crocodylus johnstoni, and Cro. porosus), thus adding to the knowledge of the role of the complement system in immunological activities of crocodilians.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53525
Siroski, Pablo Ariel; Merchant, Mark; Parachu Marco, Maria Virginia; Piña, Carlos Ignacio; Ortega, Hugo Hector; Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae); Academia Sinica; Zoological Studies; 49; 1; 1-2010; 64-70
1021-5506
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53525
identifier_str_mv Siroski, Pablo Ariel; Merchant, Mark; Parachu Marco, Maria Virginia; Piña, Carlos Ignacio; Ortega, Hugo Hector; Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae); Academia Sinica; Zoological Studies; 49; 1; 1-2010; 64-70
1021-5506
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://zoolstud.sinica.edu.tw/Journals/49.1/64.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academia Sinica
publisher.none.fl_str_mv Academia Sinica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268724540735488
score 13.13397