Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)
- Autores
- Siroski, Pablo Ariel; Merchant, Mark; Parachu Marco, Maria Virginia; Piña, Carlos Ignacio; Ortega, Hugo Hector
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Characterization of the serum complement activity of the broad-snouted caiman Caiman latirostris (Crocodilia: Alligatoridae). Zoological Studies 49(1): 64-70. The sheep red blood cell (SRBC) hemolysis assay was used to detect and characterize complement-system activity of broad-snouted caiman (Caiman latirostris) serum. The hemolytic activity of caiman serum was inhibited by 2 classic inhibitors (EDTA and heat) suggesting the existence of complement-system activity. In addition, we found that the capacity of C. latirostris serum to disrupt SRBCs was concentration, temperature, and kinetics dependent. Hemolytic activity was detected from a very low concentration (< 10%) of caiman serum and increased until 100%. Temperature influenced the activity of the serum by disrupting SRBC membranes. The serum showed a peak of hemolysis between 30 and 40°C, within which lies the optimal temperature caimans prefer during thermoregulation for normal physiological processes. Hemolytic activity rapidly occurred at 2 min, and maximum activity was detected at 60 min. These observations reflect previously reported findings in other crocodilian species (Alligator mississippiensis, Crocodylus johnstoni, and Cro. porosus), thus adding to the knowledge of the role of the complement system in immunological activities of crocodilians.
Fil: Siroski, Pablo Ariel. Provincia de Santa Fe. Secretaría de Medio Ambiente; Argentina. Proyecto Yacaré; Argentina
Fil: Merchant, Mark. McNeese State University; Estados Unidos
Fil: Parachu Marco, Maria Virginia. Proyecto Yacaré; Argentina. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Fil: Piña, Carlos Ignacio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Proyecto Yacaré; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina - Materia
-
CROCODILIANS
ECTOTHERMICS
HEMOLYTIC ASSAY
IMMUNOLOGY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/53525
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Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)Siroski, Pablo ArielMerchant, MarkParachu Marco, Maria VirginiaPiña, Carlos IgnacioOrtega, Hugo HectorCROCODILIANSECTOTHERMICSHEMOLYTIC ASSAYIMMUNOLOGYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Characterization of the serum complement activity of the broad-snouted caiman Caiman latirostris (Crocodilia: Alligatoridae). Zoological Studies 49(1): 64-70. The sheep red blood cell (SRBC) hemolysis assay was used to detect and characterize complement-system activity of broad-snouted caiman (Caiman latirostris) serum. The hemolytic activity of caiman serum was inhibited by 2 classic inhibitors (EDTA and heat) suggesting the existence of complement-system activity. In addition, we found that the capacity of C. latirostris serum to disrupt SRBCs was concentration, temperature, and kinetics dependent. Hemolytic activity was detected from a very low concentration (< 10%) of caiman serum and increased until 100%. Temperature influenced the activity of the serum by disrupting SRBC membranes. The serum showed a peak of hemolysis between 30 and 40°C, within which lies the optimal temperature caimans prefer during thermoregulation for normal physiological processes. Hemolytic activity rapidly occurred at 2 min, and maximum activity was detected at 60 min. These observations reflect previously reported findings in other crocodilian species (Alligator mississippiensis, Crocodylus johnstoni, and Cro. porosus), thus adding to the knowledge of the role of the complement system in immunological activities of crocodilians.Fil: Siroski, Pablo Ariel. Provincia de Santa Fe. Secretaría de Medio Ambiente; Argentina. Proyecto Yacaré; ArgentinaFil: Merchant, Mark. McNeese State University; Estados UnidosFil: Parachu Marco, Maria Virginia. Proyecto Yacaré; Argentina. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Piña, Carlos Ignacio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Proyecto Yacaré; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaAcademia Sinica2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53525Siroski, Pablo Ariel; Merchant, Mark; Parachu Marco, Maria Virginia; Piña, Carlos Ignacio; Ortega, Hugo Hector; Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae); Academia Sinica; Zoological Studies; 49; 1; 1-2010; 64-701021-5506CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://zoolstud.sinica.edu.tw/Journals/49.1/64.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:20Zoai:ri.conicet.gov.ar:11336/53525instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:21.151CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae) |
title |
Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae) |
spellingShingle |
Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae) Siroski, Pablo Ariel CROCODILIANS ECTOTHERMICS HEMOLYTIC ASSAY IMMUNOLOGY |
title_short |
Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae) |
title_full |
Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae) |
title_fullStr |
Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae) |
title_full_unstemmed |
Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae) |
title_sort |
Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae) |
dc.creator.none.fl_str_mv |
Siroski, Pablo Ariel Merchant, Mark Parachu Marco, Maria Virginia Piña, Carlos Ignacio Ortega, Hugo Hector |
author |
Siroski, Pablo Ariel |
author_facet |
Siroski, Pablo Ariel Merchant, Mark Parachu Marco, Maria Virginia Piña, Carlos Ignacio Ortega, Hugo Hector |
author_role |
author |
author2 |
Merchant, Mark Parachu Marco, Maria Virginia Piña, Carlos Ignacio Ortega, Hugo Hector |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
CROCODILIANS ECTOTHERMICS HEMOLYTIC ASSAY IMMUNOLOGY |
topic |
CROCODILIANS ECTOTHERMICS HEMOLYTIC ASSAY IMMUNOLOGY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Characterization of the serum complement activity of the broad-snouted caiman Caiman latirostris (Crocodilia: Alligatoridae). Zoological Studies 49(1): 64-70. The sheep red blood cell (SRBC) hemolysis assay was used to detect and characterize complement-system activity of broad-snouted caiman (Caiman latirostris) serum. The hemolytic activity of caiman serum was inhibited by 2 classic inhibitors (EDTA and heat) suggesting the existence of complement-system activity. In addition, we found that the capacity of C. latirostris serum to disrupt SRBCs was concentration, temperature, and kinetics dependent. Hemolytic activity was detected from a very low concentration (< 10%) of caiman serum and increased until 100%. Temperature influenced the activity of the serum by disrupting SRBC membranes. The serum showed a peak of hemolysis between 30 and 40°C, within which lies the optimal temperature caimans prefer during thermoregulation for normal physiological processes. Hemolytic activity rapidly occurred at 2 min, and maximum activity was detected at 60 min. These observations reflect previously reported findings in other crocodilian species (Alligator mississippiensis, Crocodylus johnstoni, and Cro. porosus), thus adding to the knowledge of the role of the complement system in immunological activities of crocodilians. Fil: Siroski, Pablo Ariel. Provincia de Santa Fe. Secretaría de Medio Ambiente; Argentina. Proyecto Yacaré; Argentina Fil: Merchant, Mark. McNeese State University; Estados Unidos Fil: Parachu Marco, Maria Virginia. Proyecto Yacaré; Argentina. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina Fil: Piña, Carlos Ignacio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Proyecto Yacaré; Argentina Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina |
description |
Characterization of the serum complement activity of the broad-snouted caiman Caiman latirostris (Crocodilia: Alligatoridae). Zoological Studies 49(1): 64-70. The sheep red blood cell (SRBC) hemolysis assay was used to detect and characterize complement-system activity of broad-snouted caiman (Caiman latirostris) serum. The hemolytic activity of caiman serum was inhibited by 2 classic inhibitors (EDTA and heat) suggesting the existence of complement-system activity. In addition, we found that the capacity of C. latirostris serum to disrupt SRBCs was concentration, temperature, and kinetics dependent. Hemolytic activity was detected from a very low concentration (< 10%) of caiman serum and increased until 100%. Temperature influenced the activity of the serum by disrupting SRBC membranes. The serum showed a peak of hemolysis between 30 and 40°C, within which lies the optimal temperature caimans prefer during thermoregulation for normal physiological processes. Hemolytic activity rapidly occurred at 2 min, and maximum activity was detected at 60 min. These observations reflect previously reported findings in other crocodilian species (Alligator mississippiensis, Crocodylus johnstoni, and Cro. porosus), thus adding to the knowledge of the role of the complement system in immunological activities of crocodilians. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/53525 Siroski, Pablo Ariel; Merchant, Mark; Parachu Marco, Maria Virginia; Piña, Carlos Ignacio; Ortega, Hugo Hector; Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae); Academia Sinica; Zoological Studies; 49; 1; 1-2010; 64-70 1021-5506 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/53525 |
identifier_str_mv |
Siroski, Pablo Ariel; Merchant, Mark; Parachu Marco, Maria Virginia; Piña, Carlos Ignacio; Ortega, Hugo Hector; Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae); Academia Sinica; Zoological Studies; 49; 1; 1-2010; 64-70 1021-5506 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://zoolstud.sinica.edu.tw/Journals/49.1/64.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academia Sinica |
publisher.none.fl_str_mv |
Academia Sinica |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268724540735488 |
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13.13397 |