Calcium-dependent protein kinase 2 is an early player in the salt stress response

Autores
Grossi, Cecilia Eugenia María; Santin, Franco; Quintana Arrúa, Silverio Andrés; Fantino, Elisa Inés; Ulloa, Rita Maria
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Potato is the most important horticultural crop in Argentina. It is well adapted to many environmental conditions but certain abiotic stresses, such as salinity, cause significant reductions in growth and yield. Understanding how potato plants perceive and combat salt stress is fundamental to develop salt-tolerant plants that could expand actual crop frontiers. When exposed to NaCl, plants exhibit fast and transient increases in cytosolic Ca2+ within seconds; this early and prominent feature needs to be decoded by calcium sensors that transduce this signal into physiological responses. In greenhouse potato plants irrigated with high salt (50 or 150 mM NaCl), shoot and root length, and shoot biomass showed a significant reduction, and photosynthetic parameters were strongly affected compared to control plants. In this report, we carried out an in silico analysis of the cis-responsive elements found in the promoter sequences of three CDPK isoforms: StCDPK1, StCDPK2, and StCDPK3. Numerous sites related to abiotic stress were predicted in the three promoters; however, these were more abundant in StCDPK2. In accordance with this, GUS activity increased in the root tips of ProStCDPK2:GUS potato plants upon salt stress. RT-qPCR assays confirmed its early induction in in vitro potato plants after 2 h of high salt exposure. Moreover, StCDPK2 expression was induced when greenhouse plants were exposed to a dynamic salt stress condition. Based on these data, we produced 35S:StCDPK2 plants to further analyze the role of this kinase under salt stress conditions. As inferred from biometric data and chlorophyll content, plants that over-express StCDPK2 were more tolerant than WT plants when exposed to high salt in vitro. Over-expressing plants showed enhanced expression of WRKY6 and ERF5 transcription factors under control conditions and reduced accumulation of peroxide and higher catalase activity under salt conditions. Our results show that StCDPK2 is an early player in the salt stress response.
Fil: Grossi, Cecilia Eugenia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Santin, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Quintana Arrúa, Silverio Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fantino, Elisa Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
Materia
StCDPK2
Calcium-dependent protein kinase
Solanum tuberosum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201591
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/201591
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Calcium-dependent protein kinase 2 is an early player in the salt stress responseGrossi, Cecilia Eugenia MaríaSantin, FrancoQuintana Arrúa, Silverio AndrésFantino, Elisa InésUlloa, Rita MariaStCDPK2Calcium-dependent protein kinaseSolanum tuberosumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Potato is the most important horticultural crop in Argentina. It is well adapted to many environmental conditions but certain abiotic stresses, such as salinity, cause significant reductions in growth and yield. Understanding how potato plants perceive and combat salt stress is fundamental to develop salt-tolerant plants that could expand actual crop frontiers. When exposed to NaCl, plants exhibit fast and transient increases in cytosolic Ca2+ within seconds; this early and prominent feature needs to be decoded by calcium sensors that transduce this signal into physiological responses. In greenhouse potato plants irrigated with high salt (50 or 150 mM NaCl), shoot and root length, and shoot biomass showed a significant reduction, and photosynthetic parameters were strongly affected compared to control plants. In this report, we carried out an in silico analysis of the cis-responsive elements found in the promoter sequences of three CDPK isoforms: StCDPK1, StCDPK2, and StCDPK3. Numerous sites related to abiotic stress were predicted in the three promoters; however, these were more abundant in StCDPK2. In accordance with this, GUS activity increased in the root tips of ProStCDPK2:GUS potato plants upon salt stress. RT-qPCR assays confirmed its early induction in in vitro potato plants after 2 h of high salt exposure. Moreover, StCDPK2 expression was induced when greenhouse plants were exposed to a dynamic salt stress condition. Based on these data, we produced 35S:StCDPK2 plants to further analyze the role of this kinase under salt stress conditions. As inferred from biometric data and chlorophyll content, plants that over-express StCDPK2 were more tolerant than WT plants when exposed to high salt in vitro. Over-expressing plants showed enhanced expression of WRKY6 and ERF5 transcription factors under control conditions and reduced accumulation of peroxide and higher catalase activity under salt conditions. Our results show that StCDPK2 is an early player in the salt stress response.Fil: Grossi, Cecilia Eugenia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Santin, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Quintana Arrúa, Silverio Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Fantino, Elisa Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaLVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General MicrobiologyCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201591Calcium-dependent protein kinase 2 is an early player in the salt stress response; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 140-1400327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/hdl/https://ri.conicet.gov.ar/handle/11336/139095info:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/170-TSP_BIOCELL_42376.pdfinfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-45.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:33Zoai:ri.conicet.gov.ar:11336/201591instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:33.824CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Calcium-dependent protein kinase 2 is an early player in the salt stress response
title Calcium-dependent protein kinase 2 is an early player in the salt stress response
spellingShingle Calcium-dependent protein kinase 2 is an early player in the salt stress response
Grossi, Cecilia Eugenia María
StCDPK2
Calcium-dependent protein kinase
Solanum tuberosum
title_short Calcium-dependent protein kinase 2 is an early player in the salt stress response
title_full Calcium-dependent protein kinase 2 is an early player in the salt stress response
title_fullStr Calcium-dependent protein kinase 2 is an early player in the salt stress response
title_full_unstemmed Calcium-dependent protein kinase 2 is an early player in the salt stress response
title_sort Calcium-dependent protein kinase 2 is an early player in the salt stress response
dc.creator.none.fl_str_mv Grossi, Cecilia Eugenia María
Santin, Franco
Quintana Arrúa, Silverio Andrés
Fantino, Elisa Inés
Ulloa, Rita Maria
author Grossi, Cecilia Eugenia María
author_facet Grossi, Cecilia Eugenia María
Santin, Franco
Quintana Arrúa, Silverio Andrés
Fantino, Elisa Inés
Ulloa, Rita Maria
author_role author
author2 Santin, Franco
Quintana Arrúa, Silverio Andrés
Fantino, Elisa Inés
Ulloa, Rita Maria
author2_role author
author
author
author
dc.subject.none.fl_str_mv StCDPK2
Calcium-dependent protein kinase
Solanum tuberosum
topic StCDPK2
Calcium-dependent protein kinase
Solanum tuberosum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Potato is the most important horticultural crop in Argentina. It is well adapted to many environmental conditions but certain abiotic stresses, such as salinity, cause significant reductions in growth and yield. Understanding how potato plants perceive and combat salt stress is fundamental to develop salt-tolerant plants that could expand actual crop frontiers. When exposed to NaCl, plants exhibit fast and transient increases in cytosolic Ca2+ within seconds; this early and prominent feature needs to be decoded by calcium sensors that transduce this signal into physiological responses. In greenhouse potato plants irrigated with high salt (50 or 150 mM NaCl), shoot and root length, and shoot biomass showed a significant reduction, and photosynthetic parameters were strongly affected compared to control plants. In this report, we carried out an in silico analysis of the cis-responsive elements found in the promoter sequences of three CDPK isoforms: StCDPK1, StCDPK2, and StCDPK3. Numerous sites related to abiotic stress were predicted in the three promoters; however, these were more abundant in StCDPK2. In accordance with this, GUS activity increased in the root tips of ProStCDPK2:GUS potato plants upon salt stress. RT-qPCR assays confirmed its early induction in in vitro potato plants after 2 h of high salt exposure. Moreover, StCDPK2 expression was induced when greenhouse plants were exposed to a dynamic salt stress condition. Based on these data, we produced 35S:StCDPK2 plants to further analyze the role of this kinase under salt stress conditions. As inferred from biometric data and chlorophyll content, plants that over-express StCDPK2 were more tolerant than WT plants when exposed to high salt in vitro. Over-expressing plants showed enhanced expression of WRKY6 and ERF5 transcription factors under control conditions and reduced accumulation of peroxide and higher catalase activity under salt conditions. Our results show that StCDPK2 is an early player in the salt stress response.
Fil: Grossi, Cecilia Eugenia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Santin, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Quintana Arrúa, Silverio Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fantino, Elisa Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
description Potato is the most important horticultural crop in Argentina. It is well adapted to many environmental conditions but certain abiotic stresses, such as salinity, cause significant reductions in growth and yield. Understanding how potato plants perceive and combat salt stress is fundamental to develop salt-tolerant plants that could expand actual crop frontiers. When exposed to NaCl, plants exhibit fast and transient increases in cytosolic Ca2+ within seconds; this early and prominent feature needs to be decoded by calcium sensors that transduce this signal into physiological responses. In greenhouse potato plants irrigated with high salt (50 or 150 mM NaCl), shoot and root length, and shoot biomass showed a significant reduction, and photosynthetic parameters were strongly affected compared to control plants. In this report, we carried out an in silico analysis of the cis-responsive elements found in the promoter sequences of three CDPK isoforms: StCDPK1, StCDPK2, and StCDPK3. Numerous sites related to abiotic stress were predicted in the three promoters; however, these were more abundant in StCDPK2. In accordance with this, GUS activity increased in the root tips of ProStCDPK2:GUS potato plants upon salt stress. RT-qPCR assays confirmed its early induction in in vitro potato plants after 2 h of high salt exposure. Moreover, StCDPK2 expression was induced when greenhouse plants were exposed to a dynamic salt stress condition. Based on these data, we produced 35S:StCDPK2 plants to further analyze the role of this kinase under salt stress conditions. As inferred from biometric data and chlorophyll content, plants that over-express StCDPK2 were more tolerant than WT plants when exposed to high salt in vitro. Over-expressing plants showed enhanced expression of WRKY6 and ERF5 transcription factors under control conditions and reduced accumulation of peroxide and higher catalase activity under salt conditions. Our results show that StCDPK2 is an early player in the salt stress response.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201591
Calcium-dependent protein kinase 2 is an early player in the salt stress response; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 140-140
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201591
identifier_str_mv Calcium-dependent protein kinase 2 is an early player in the salt stress response; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 140-140
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/170-TSP_BIOCELL_42376.pdf
info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-45.pdf
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application/pdf
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dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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