Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats

Autores
Adilardi, Renzo Sebastián; Ojanguren Affilastro, Andres Alejandro; Mattoni, Camilo Ivan; Mola, Liliana Maria
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
All cytogenetically studied scorpions present male achiasmatic meiosis and lack heteromorphic sex chromosomes. In contrast, information about female meiosis in scorpions is scarce due to the difficulty of finding meiotic cells. The genus Zabius includes three described species and no chromosome studies have been performed on it until now. We analyzed the constitutive heterochromatin distribution, NORs and telomeric sequences in mitosis and meiosis of males and females of different populations of Zabius fuscus. All specimens presented 2n=18 holokinetic chromosomes that gradually decreased in size. Male meiosis presented nine bivalents and a polymorphism for one reciprocal translocation in one population. Telomeric signals were detected at every terminal region, confirming also the presence of a (TTAGG)n motif in Buthidae. Constitutive heterochromatin was found in three chromosome pairs at a terminal region; moreover, NORs were embedded in the heterochromatic region of the largest pair. Chromosome size and landmarks allowed us to propose the chromosomes involved in the rearrangement. In four females, cells at different prophase I stages were analyzed. We describe a diffuse stage and the presence of ring-shaped bivalents. We discuss the possible origin of these bivalents in the framework of chiasmatic or achiasmatic female meiosis. These results contribute to increase the scarce evidence of female meiosis in scorpions and raise new questions about its mechanism.
Fil: Adilardi, Renzo Sebastián. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Ojanguren Affilastro, Andres Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales; Argentina
Fil: Mattoni, Camilo Ivan. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fil: Mola, Liliana Maria. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Materia
Holokinetic Chromosomes
Achiasmatic Meiosis
Telomere
Fish
Female Diffuse Stage
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7936
Acceder
id CONICETDig_3df8ca4bbf86c730c1beb5a4aa65d393
oai_identifier_str oai:ri.conicet.gov.ar:11336/7936
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeatsAdilardi, Renzo SebastiánOjanguren Affilastro, Andres AlejandroMattoni, Camilo IvanMola, Liliana MariaHolokinetic ChromosomesAchiasmatic MeiosisTelomereFishFemale Diffuse Stagehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1All cytogenetically studied scorpions present male achiasmatic meiosis and lack heteromorphic sex chromosomes. In contrast, information about female meiosis in scorpions is scarce due to the difficulty of finding meiotic cells. The genus Zabius includes three described species and no chromosome studies have been performed on it until now. We analyzed the constitutive heterochromatin distribution, NORs and telomeric sequences in mitosis and meiosis of males and females of different populations of Zabius fuscus. All specimens presented 2n=18 holokinetic chromosomes that gradually decreased in size. Male meiosis presented nine bivalents and a polymorphism for one reciprocal translocation in one population. Telomeric signals were detected at every terminal region, confirming also the presence of a (TTAGG)n motif in Buthidae. Constitutive heterochromatin was found in three chromosome pairs at a terminal region; moreover, NORs were embedded in the heterochromatic region of the largest pair. Chromosome size and landmarks allowed us to propose the chromosomes involved in the rearrangement. In four females, cells at different prophase I stages were analyzed. We describe a diffuse stage and the presence of ring-shaped bivalents. We discuss the possible origin of these bivalents in the framework of chiasmatic or achiasmatic female meiosis. These results contribute to increase the scarce evidence of female meiosis in scorpions and raise new questions about its mechanism.Fil: Adilardi, Renzo Sebastián. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Ojanguren Affilastro, Andres Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales; ArgentinaFil: Mattoni, Camilo Ivan. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Mola, Liliana Maria. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaSpringer2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7936Adilardi, Renzo Sebastián; Ojanguren Affilastro, Andres Alejandro; Mattoni, Camilo Ivan; Mola, Liliana Maria; Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats; Springer; Genetica; 143; 4; 4-2015; 393-4010016-6707enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s10709-015-9838-1info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1007/s10709-015-9838-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:27:38Zoai:ri.conicet.gov.ar:11336/7936instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:27:39.044CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats
title Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats
spellingShingle Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats
Adilardi, Renzo Sebastián
Holokinetic Chromosomes
Achiasmatic Meiosis
Telomere
Fish
Female Diffuse Stage
title_short Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats
title_full Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats
title_fullStr Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats
title_full_unstemmed Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats
title_sort Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats
dc.creator.none.fl_str_mv Adilardi, Renzo Sebastián
Ojanguren Affilastro, Andres Alejandro
Mattoni, Camilo Ivan
Mola, Liliana Maria
author Adilardi, Renzo Sebastián
author_facet Adilardi, Renzo Sebastián
Ojanguren Affilastro, Andres Alejandro
Mattoni, Camilo Ivan
Mola, Liliana Maria
author_role author
author2 Ojanguren Affilastro, Andres Alejandro
Mattoni, Camilo Ivan
Mola, Liliana Maria
author2_role author
author
author
dc.subject.none.fl_str_mv Holokinetic Chromosomes
Achiasmatic Meiosis
Telomere
Fish
Female Diffuse Stage
topic Holokinetic Chromosomes
Achiasmatic Meiosis
Telomere
Fish
Female Diffuse Stage
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv All cytogenetically studied scorpions present male achiasmatic meiosis and lack heteromorphic sex chromosomes. In contrast, information about female meiosis in scorpions is scarce due to the difficulty of finding meiotic cells. The genus Zabius includes three described species and no chromosome studies have been performed on it until now. We analyzed the constitutive heterochromatin distribution, NORs and telomeric sequences in mitosis and meiosis of males and females of different populations of Zabius fuscus. All specimens presented 2n=18 holokinetic chromosomes that gradually decreased in size. Male meiosis presented nine bivalents and a polymorphism for one reciprocal translocation in one population. Telomeric signals were detected at every terminal region, confirming also the presence of a (TTAGG)n motif in Buthidae. Constitutive heterochromatin was found in three chromosome pairs at a terminal region; moreover, NORs were embedded in the heterochromatic region of the largest pair. Chromosome size and landmarks allowed us to propose the chromosomes involved in the rearrangement. In four females, cells at different prophase I stages were analyzed. We describe a diffuse stage and the presence of ring-shaped bivalents. We discuss the possible origin of these bivalents in the framework of chiasmatic or achiasmatic female meiosis. These results contribute to increase the scarce evidence of female meiosis in scorpions and raise new questions about its mechanism.
Fil: Adilardi, Renzo Sebastián. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Ojanguren Affilastro, Andres Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales; Argentina
Fil: Mattoni, Camilo Ivan. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fil: Mola, Liliana Maria. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
description All cytogenetically studied scorpions present male achiasmatic meiosis and lack heteromorphic sex chromosomes. In contrast, information about female meiosis in scorpions is scarce due to the difficulty of finding meiotic cells. The genus Zabius includes three described species and no chromosome studies have been performed on it until now. We analyzed the constitutive heterochromatin distribution, NORs and telomeric sequences in mitosis and meiosis of males and females of different populations of Zabius fuscus. All specimens presented 2n=18 holokinetic chromosomes that gradually decreased in size. Male meiosis presented nine bivalents and a polymorphism for one reciprocal translocation in one population. Telomeric signals were detected at every terminal region, confirming also the presence of a (TTAGG)n motif in Buthidae. Constitutive heterochromatin was found in three chromosome pairs at a terminal region; moreover, NORs were embedded in the heterochromatic region of the largest pair. Chromosome size and landmarks allowed us to propose the chromosomes involved in the rearrangement. In four females, cells at different prophase I stages were analyzed. We describe a diffuse stage and the presence of ring-shaped bivalents. We discuss the possible origin of these bivalents in the framework of chiasmatic or achiasmatic female meiosis. These results contribute to increase the scarce evidence of female meiosis in scorpions and raise new questions about its mechanism.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7936
Adilardi, Renzo Sebastián; Ojanguren Affilastro, Andres Alejandro; Mattoni, Camilo Ivan; Mola, Liliana Maria; Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats; Springer; Genetica; 143; 4; 4-2015; 393-401
0016-6707
url http://hdl.handle.net/11336/7936
identifier_str_mv Adilardi, Renzo Sebastián; Ojanguren Affilastro, Andres Alejandro; Mattoni, Camilo Ivan; Mola, Liliana Maria; Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): Heterochromatin, rDNA and TTAGG telomeric repeats; Springer; Genetica; 143; 4; 4-2015; 393-401
0016-6707
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s10709-015-9838-1
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1007/s10709-015-9838-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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