Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis

Autores
Machado, Mizael; Castro, Márcio B.; Wilson, Tais M.; Gonçalves, Alexandra A. B.; Portiansky, Enrique Leo; Riet Correa, Franklin; Barros, Severo S.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vascular mineralization is a hallmark of enzootic calcinosis. Histopathological, ultrastructural, and immunohistochemical investigations were performed on the external carotid arteries of seven sheep naturally poisoned by Nierembergia veitchii. Histologically, moderate to marked hyperplasia of the tunica intima was observed without mineralization. The tunica media exhibited mild to severe mineralization and osteochondroid metaplasia. Sheep with enzootic calcinosis showed arterial overexpression of osteopontin and tissue-nonspecific alkaline phosphatase and immunolabeling for osteonectin and osteocalcin in both intima and media layers of the tested arteries. The main ultrastructural finding in the tunica media was a marked phenotypic change of vascular smooth muscle cells from a contractile phenotype (VSMC-C) into a synthetic phenotype (VSMC-S). In the tunica media, VSMC-S produced matrix and extracellular vesicles, forming mineralizable granules associated with arterial mineralization. VSMC-S were also present in the tunica intima, but matrix and extracellular vesicles and mineralization were not observed. The absence of matrix and extracellular vesicles in the intimal hyperplasia, even in the presence of noncollagenous bone proteins, tissue-nonspecific alkaline phosphatase, and vitamin D receptors, reinforces the hypothesis that the presence of matrix and extracellular vesicles are crucial for the development of vascular mineralization in enzootic calcinosis. It is proposed that the two different VSMC-S phenotypes in calcinosis are due to the expression of at least two genetically different types of these cells induced by the action of 1,25(OH)2D3.
Fil: Machado, Mizael. Instituto Nacional de Investigación Agropecuaria Inia Uruguay; Uruguay
Fil: Castro, Márcio B.. Universidade do Brasília; Brasil
Fil: Wilson, Tais M.. Universidade do Brasília; Brasil
Fil: Gonçalves, Alexandra A. B.. Universidade do Brasília; Brasil
Fil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Riet Correa, Franklin. Instituto Nacional de Investigación Agropecuaria Inia Uruguay; Uruguay. Universidade Federal da Bahia; Brasil
Fil: Barros, Severo S.. Universidade Federal de Santa Maria; Brasil
Materia
ARTERIAL MINERALIZATION
ARTERIES
ENZOOTIC CALCINOSIS
EXOSOMES
PATHOGENESIS
TOXICITY
VASCULAR SMOOTH MUSCLE CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/218364
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/218364
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosisMachado, MizaelCastro, Márcio B.Wilson, Tais M.Gonçalves, Alexandra A. B.Portiansky, Enrique LeoRiet Correa, FranklinBarros, Severo S.ARTERIAL MINERALIZATIONARTERIESENZOOTIC CALCINOSISEXOSOMESPATHOGENESISTOXICITYVASCULAR SMOOTH MUSCLE CELLShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Vascular mineralization is a hallmark of enzootic calcinosis. Histopathological, ultrastructural, and immunohistochemical investigations were performed on the external carotid arteries of seven sheep naturally poisoned by Nierembergia veitchii. Histologically, moderate to marked hyperplasia of the tunica intima was observed without mineralization. The tunica media exhibited mild to severe mineralization and osteochondroid metaplasia. Sheep with enzootic calcinosis showed arterial overexpression of osteopontin and tissue-nonspecific alkaline phosphatase and immunolabeling for osteonectin and osteocalcin in both intima and media layers of the tested arteries. The main ultrastructural finding in the tunica media was a marked phenotypic change of vascular smooth muscle cells from a contractile phenotype (VSMC-C) into a synthetic phenotype (VSMC-S). In the tunica media, VSMC-S produced matrix and extracellular vesicles, forming mineralizable granules associated with arterial mineralization. VSMC-S were also present in the tunica intima, but matrix and extracellular vesicles and mineralization were not observed. The absence of matrix and extracellular vesicles in the intimal hyperplasia, even in the presence of noncollagenous bone proteins, tissue-nonspecific alkaline phosphatase, and vitamin D receptors, reinforces the hypothesis that the presence of matrix and extracellular vesicles are crucial for the development of vascular mineralization in enzootic calcinosis. It is proposed that the two different VSMC-S phenotypes in calcinosis are due to the expression of at least two genetically different types of these cells induced by the action of 1,25(OH)2D3.Fil: Machado, Mizael. Instituto Nacional de Investigación Agropecuaria Inia Uruguay; UruguayFil: Castro, Márcio B.. Universidade do Brasília; BrasilFil: Wilson, Tais M.. Universidade do Brasília; BrasilFil: Gonçalves, Alexandra A. B.. Universidade do Brasília; BrasilFil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Riet Correa, Franklin. Instituto Nacional de Investigación Agropecuaria Inia Uruguay; Uruguay. Universidade Federal da Bahia; BrasilFil: Barros, Severo S.. Universidade Federal de Santa Maria; BrasilAmerican College of Veterinary Pathologists2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/218364Machado, Mizael; Castro, Márcio B.; Wilson, Tais M.; Gonçalves, Alexandra A. B.; Portiansky, Enrique Leo; et al.; Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis; American College of Veterinary Pathologists; Veterinary Pathology; 59; 5; 9-2022; 814-8230300-9858CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1177/03009858221098430info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/03009858221098430info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:28Zoai:ri.conicet.gov.ar:11336/218364instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:29.023CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis
title Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis
spellingShingle Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis
Machado, Mizael
ARTERIAL MINERALIZATION
ARTERIES
ENZOOTIC CALCINOSIS
EXOSOMES
PATHOGENESIS
TOXICITY
VASCULAR SMOOTH MUSCLE CELLS
title_short Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis
title_full Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis
title_fullStr Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis
title_full_unstemmed Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis
title_sort Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis
dc.creator.none.fl_str_mv Machado, Mizael
Castro, Márcio B.
Wilson, Tais M.
Gonçalves, Alexandra A. B.
Portiansky, Enrique Leo
Riet Correa, Franklin
Barros, Severo S.
author Machado, Mizael
author_facet Machado, Mizael
Castro, Márcio B.
Wilson, Tais M.
Gonçalves, Alexandra A. B.
Portiansky, Enrique Leo
Riet Correa, Franklin
Barros, Severo S.
author_role author
author2 Castro, Márcio B.
Wilson, Tais M.
Gonçalves, Alexandra A. B.
Portiansky, Enrique Leo
Riet Correa, Franklin
Barros, Severo S.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ARTERIAL MINERALIZATION
ARTERIES
ENZOOTIC CALCINOSIS
EXOSOMES
PATHOGENESIS
TOXICITY
VASCULAR SMOOTH MUSCLE CELLS
topic ARTERIAL MINERALIZATION
ARTERIES
ENZOOTIC CALCINOSIS
EXOSOMES
PATHOGENESIS
TOXICITY
VASCULAR SMOOTH MUSCLE CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Vascular mineralization is a hallmark of enzootic calcinosis. Histopathological, ultrastructural, and immunohistochemical investigations were performed on the external carotid arteries of seven sheep naturally poisoned by Nierembergia veitchii. Histologically, moderate to marked hyperplasia of the tunica intima was observed without mineralization. The tunica media exhibited mild to severe mineralization and osteochondroid metaplasia. Sheep with enzootic calcinosis showed arterial overexpression of osteopontin and tissue-nonspecific alkaline phosphatase and immunolabeling for osteonectin and osteocalcin in both intima and media layers of the tested arteries. The main ultrastructural finding in the tunica media was a marked phenotypic change of vascular smooth muscle cells from a contractile phenotype (VSMC-C) into a synthetic phenotype (VSMC-S). In the tunica media, VSMC-S produced matrix and extracellular vesicles, forming mineralizable granules associated with arterial mineralization. VSMC-S were also present in the tunica intima, but matrix and extracellular vesicles and mineralization were not observed. The absence of matrix and extracellular vesicles in the intimal hyperplasia, even in the presence of noncollagenous bone proteins, tissue-nonspecific alkaline phosphatase, and vitamin D receptors, reinforces the hypothesis that the presence of matrix and extracellular vesicles are crucial for the development of vascular mineralization in enzootic calcinosis. It is proposed that the two different VSMC-S phenotypes in calcinosis are due to the expression of at least two genetically different types of these cells induced by the action of 1,25(OH)2D3.
Fil: Machado, Mizael. Instituto Nacional de Investigación Agropecuaria Inia Uruguay; Uruguay
Fil: Castro, Márcio B.. Universidade do Brasília; Brasil
Fil: Wilson, Tais M.. Universidade do Brasília; Brasil
Fil: Gonçalves, Alexandra A. B.. Universidade do Brasília; Brasil
Fil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Riet Correa, Franklin. Instituto Nacional de Investigación Agropecuaria Inia Uruguay; Uruguay. Universidade Federal da Bahia; Brasil
Fil: Barros, Severo S.. Universidade Federal de Santa Maria; Brasil
description Vascular mineralization is a hallmark of enzootic calcinosis. Histopathological, ultrastructural, and immunohistochemical investigations were performed on the external carotid arteries of seven sheep naturally poisoned by Nierembergia veitchii. Histologically, moderate to marked hyperplasia of the tunica intima was observed without mineralization. The tunica media exhibited mild to severe mineralization and osteochondroid metaplasia. Sheep with enzootic calcinosis showed arterial overexpression of osteopontin and tissue-nonspecific alkaline phosphatase and immunolabeling for osteonectin and osteocalcin in both intima and media layers of the tested arteries. The main ultrastructural finding in the tunica media was a marked phenotypic change of vascular smooth muscle cells from a contractile phenotype (VSMC-C) into a synthetic phenotype (VSMC-S). In the tunica media, VSMC-S produced matrix and extracellular vesicles, forming mineralizable granules associated with arterial mineralization. VSMC-S were also present in the tunica intima, but matrix and extracellular vesicles and mineralization were not observed. The absence of matrix and extracellular vesicles in the intimal hyperplasia, even in the presence of noncollagenous bone proteins, tissue-nonspecific alkaline phosphatase, and vitamin D receptors, reinforces the hypothesis that the presence of matrix and extracellular vesicles are crucial for the development of vascular mineralization in enzootic calcinosis. It is proposed that the two different VSMC-S phenotypes in calcinosis are due to the expression of at least two genetically different types of these cells induced by the action of 1,25(OH)2D3.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/218364
Machado, Mizael; Castro, Márcio B.; Wilson, Tais M.; Gonçalves, Alexandra A. B.; Portiansky, Enrique Leo; et al.; Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis; American College of Veterinary Pathologists; Veterinary Pathology; 59; 5; 9-2022; 814-823
0300-9858
CONICET Digital
CONICET
url http://hdl.handle.net/11336/218364
identifier_str_mv Machado, Mizael; Castro, Márcio B.; Wilson, Tais M.; Gonçalves, Alexandra A. B.; Portiansky, Enrique Leo; et al.; Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis; American College of Veterinary Pathologists; Veterinary Pathology; 59; 5; 9-2022; 814-823
0300-9858
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1177/03009858221098430
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/03009858221098430
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American College of Veterinary Pathologists
publisher.none.fl_str_mv American College of Veterinary Pathologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269697327759360
score 13.13397

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