Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia

Autores
Zamponi, Nahuel; Zamponi, Emiliano; Mayol, Gonzalo Federico; Lanfredi Rangel, Adriana; Svärd, Staffan G; Touz, Maria Carolina
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Our understanding of protein and lipid trafficking in eukaryotic cells has been challenged by the finding of different forms of compartmentalization and cargo processing in protozoan parasites. Here, we show that, in the absence of a Golgi compartment in Giardia, proteins destined for secretion are directly sorted and packaged at specialized ER regions enriched in COPII coatomer complexes and ceramide. We also demonstrated that ER-resident proteins are retained at the ER by the action of a KDEL receptor, which, in contrast to other eukaryotic KDEL receptors, showed no interorganellar dynamic but instead acts specifically at the limit of the ER membrane. Our study suggests that the ER-exit sites and the perinuclear ER-membranes are capable of performing protein-sorting functions. In our view, the description presented here suggests that Giardia adaptation represents an extreme example of reductive evolution without loss of function.
Fil: Zamponi, Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Zamponi, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Mayol, Gonzalo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Lanfredi Rangel, Adriana. Centro de Pesquisas "Gonçalo Moniz"; Brasil
Fil: Svärd, Staffan G. Uppsala University; Suecia
Fil: Touz, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Materia
Ceramide
Coatomer
Encystation
Secretory Pathway
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63510
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/63510
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lambliaZamponi, NahuelZamponi, EmilianoMayol, Gonzalo FedericoLanfredi Rangel, AdrianaSvärd, Staffan GTouz, Maria CarolinaCeramideCoatomerEncystationSecretory Pathwayhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Our understanding of protein and lipid trafficking in eukaryotic cells has been challenged by the finding of different forms of compartmentalization and cargo processing in protozoan parasites. Here, we show that, in the absence of a Golgi compartment in Giardia, proteins destined for secretion are directly sorted and packaged at specialized ER regions enriched in COPII coatomer complexes and ceramide. We also demonstrated that ER-resident proteins are retained at the ER by the action of a KDEL receptor, which, in contrast to other eukaryotic KDEL receptors, showed no interorganellar dynamic but instead acts specifically at the limit of the ER membrane. Our study suggests that the ER-exit sites and the perinuclear ER-membranes are capable of performing protein-sorting functions. In our view, the description presented here suggests that Giardia adaptation represents an extreme example of reductive evolution without loss of function.Fil: Zamponi, Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Zamponi, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Mayol, Gonzalo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Lanfredi Rangel, Adriana. Centro de Pesquisas "Gonçalo Moniz"; BrasilFil: Svärd, Staffan G. Uppsala University; SueciaFil: Touz, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaWiley Blackwell Publishing, Inc2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63510Zamponi, Nahuel; Zamponi, Emiliano; Mayol, Gonzalo Federico; Lanfredi Rangel, Adriana; Svärd, Staffan G; et al.; Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia; Wiley Blackwell Publishing, Inc; Traffic; 18; 9; 9-2017; 604-6211398-92191600-0854CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/tra.12501info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/tra.12501info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:44Zoai:ri.conicet.gov.ar:11336/63510instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:44.33CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia
title Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia
spellingShingle Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia
Zamponi, Nahuel
Ceramide
Coatomer
Encystation
Secretory Pathway
title_short Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia
title_full Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia
title_fullStr Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia
title_full_unstemmed Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia
title_sort Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia
dc.creator.none.fl_str_mv Zamponi, Nahuel
Zamponi, Emiliano
Mayol, Gonzalo Federico
Lanfredi Rangel, Adriana
Svärd, Staffan G
Touz, Maria Carolina
author Zamponi, Nahuel
author_facet Zamponi, Nahuel
Zamponi, Emiliano
Mayol, Gonzalo Federico
Lanfredi Rangel, Adriana
Svärd, Staffan G
Touz, Maria Carolina
author_role author
author2 Zamponi, Emiliano
Mayol, Gonzalo Federico
Lanfredi Rangel, Adriana
Svärd, Staffan G
Touz, Maria Carolina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ceramide
Coatomer
Encystation
Secretory Pathway
topic Ceramide
Coatomer
Encystation
Secretory Pathway
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Our understanding of protein and lipid trafficking in eukaryotic cells has been challenged by the finding of different forms of compartmentalization and cargo processing in protozoan parasites. Here, we show that, in the absence of a Golgi compartment in Giardia, proteins destined for secretion are directly sorted and packaged at specialized ER regions enriched in COPII coatomer complexes and ceramide. We also demonstrated that ER-resident proteins are retained at the ER by the action of a KDEL receptor, which, in contrast to other eukaryotic KDEL receptors, showed no interorganellar dynamic but instead acts specifically at the limit of the ER membrane. Our study suggests that the ER-exit sites and the perinuclear ER-membranes are capable of performing protein-sorting functions. In our view, the description presented here suggests that Giardia adaptation represents an extreme example of reductive evolution without loss of function.
Fil: Zamponi, Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Zamponi, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Mayol, Gonzalo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Lanfredi Rangel, Adriana. Centro de Pesquisas "Gonçalo Moniz"; Brasil
Fil: Svärd, Staffan G. Uppsala University; Suecia
Fil: Touz, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
description Our understanding of protein and lipid trafficking in eukaryotic cells has been challenged by the finding of different forms of compartmentalization and cargo processing in protozoan parasites. Here, we show that, in the absence of a Golgi compartment in Giardia, proteins destined for secretion are directly sorted and packaged at specialized ER regions enriched in COPII coatomer complexes and ceramide. We also demonstrated that ER-resident proteins are retained at the ER by the action of a KDEL receptor, which, in contrast to other eukaryotic KDEL receptors, showed no interorganellar dynamic but instead acts specifically at the limit of the ER membrane. Our study suggests that the ER-exit sites and the perinuclear ER-membranes are capable of performing protein-sorting functions. In our view, the description presented here suggests that Giardia adaptation represents an extreme example of reductive evolution without loss of function.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63510
Zamponi, Nahuel; Zamponi, Emiliano; Mayol, Gonzalo Federico; Lanfredi Rangel, Adriana; Svärd, Staffan G; et al.; Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia; Wiley Blackwell Publishing, Inc; Traffic; 18; 9; 9-2017; 604-621
1398-9219
1600-0854
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63510
identifier_str_mv Zamponi, Nahuel; Zamponi, Emiliano; Mayol, Gonzalo Federico; Lanfredi Rangel, Adriana; Svärd, Staffan G; et al.; Endoplasmic reticulum is the sorting core facility in the Golgi-lacking protozoan Giardia lamblia; Wiley Blackwell Publishing, Inc; Traffic; 18; 9; 9-2017; 604-621
1398-9219
1600-0854
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/tra.12501
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/tra.12501
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613876309557248
score 13.070432

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