Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation

Autores
Giron, Santiago; Tejera, Agueda M.; Ripoll, Giselle Vanina; Gomez, Daniel Eduardo; Alonso, Daniel Fernando
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background and Objectives: Desmopressin (DDAVP) is a synthetic derivative of vasopressin with hemostatic and fibrinolytic properties that has been used during surgery in patients with bleeding disorders. Our aim was to investigate the effect of DDAVP on lung and lymph node metastatic cell colonization using a preclinical mouse mammary carcinoma model of subcutaneous tumor manipulation and surgical excision. Methods: Female BALB/c mice bearing the highly aggressive F3II mammary carcinoma were subjected to repeated manipulations of primary tumors (0.5 kg/cm2 during 2 min), followed (or not) by surgical excision. DDAVP was administered intravenously 30 min before and 24 h after each manipulation or surgery, at a dose of 2 μg/kg. At the end of the experiment, mice were sacrificed and necropsied. Results: Tumor manipulation induced dissemination to the axillary nodes and increased up to 6‐fold the number of metastatic lung nodules. Perioperative treatment with DDAVP dramatically reduced regional metastasis. The incidence of lymph node involvement in manipulated animals was 12% with DDAVP and 87% without treatment (P < 0.02). Histopathological analysis of axillary nodes from DDAVP‐treated animals showed sinusal histiocytosis and no evidence of cancer cells. Metastatic lung nodules were also reduced about 65% in animals treated with DDAVP (P = 0.026). Conclusions: Our results suggest a potential clinical application of DDAVP in the management of breast cancer, as well as other aggressive solid tumors. DDAVP may be useful to reduce the risk of metastatic cell colonization both during and after surgical manipulation.
Fil: Giron, Santiago. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina
Fil: Tejera, Agueda M.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina
Fil: Ripoll, Giselle Vanina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Breast Cancer
Metastasis
Surgery
Treatment
Vasopressin Analogue
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43483
Acceder
id CONICETDig_3d19c270fcf6d1bb47a779137016af86
oai_identifier_str oai:ri.conicet.gov.ar:11336/43483
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulationGiron, SantiagoTejera, Agueda M.Ripoll, Giselle VaninaGomez, Daniel EduardoAlonso, Daniel FernandoBreast CancerMetastasisSurgeryTreatmentVasopressin Analoguehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background and Objectives: Desmopressin (DDAVP) is a synthetic derivative of vasopressin with hemostatic and fibrinolytic properties that has been used during surgery in patients with bleeding disorders. Our aim was to investigate the effect of DDAVP on lung and lymph node metastatic cell colonization using a preclinical mouse mammary carcinoma model of subcutaneous tumor manipulation and surgical excision. Methods: Female BALB/c mice bearing the highly aggressive F3II mammary carcinoma were subjected to repeated manipulations of primary tumors (0.5 kg/cm2 during 2 min), followed (or not) by surgical excision. DDAVP was administered intravenously 30 min before and 24 h after each manipulation or surgery, at a dose of 2 μg/kg. At the end of the experiment, mice were sacrificed and necropsied. Results: Tumor manipulation induced dissemination to the axillary nodes and increased up to 6‐fold the number of metastatic lung nodules. Perioperative treatment with DDAVP dramatically reduced regional metastasis. The incidence of lymph node involvement in manipulated animals was 12% with DDAVP and 87% without treatment (P < 0.02). Histopathological analysis of axillary nodes from DDAVP‐treated animals showed sinusal histiocytosis and no evidence of cancer cells. Metastatic lung nodules were also reduced about 65% in animals treated with DDAVP (P = 0.026). Conclusions: Our results suggest a potential clinical application of DDAVP in the management of breast cancer, as well as other aggressive solid tumors. DDAVP may be useful to reduce the risk of metastatic cell colonization both during and after surgical manipulation.Fil: Giron, Santiago. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; ArgentinaFil: Tejera, Agueda M.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; ArgentinaFil: Ripoll, Giselle Vanina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley-liss, Div John Wiley & Sons Inc2002-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43483Giron, Santiago; Tejera, Agueda M.; Ripoll, Giselle Vanina; Gomez, Daniel Eduardo; Alonso, Daniel Fernando; Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation; Wiley-liss, Div John Wiley & Sons Inc; Journal of Surgical Oncology; 81; 1; 9-2002; 38-440022-4790CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jso.10141info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jso.10141info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:37Zoai:ri.conicet.gov.ar:11336/43483instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:37.642CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation
title Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation
spellingShingle Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation
Giron, Santiago
Breast Cancer
Metastasis
Surgery
Treatment
Vasopressin Analogue
title_short Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation
title_full Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation
title_fullStr Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation
title_full_unstemmed Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation
title_sort Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation
dc.creator.none.fl_str_mv Giron, Santiago
Tejera, Agueda M.
Ripoll, Giselle Vanina
Gomez, Daniel Eduardo
Alonso, Daniel Fernando
author Giron, Santiago
author_facet Giron, Santiago
Tejera, Agueda M.
Ripoll, Giselle Vanina
Gomez, Daniel Eduardo
Alonso, Daniel Fernando
author_role author
author2 Tejera, Agueda M.
Ripoll, Giselle Vanina
Gomez, Daniel Eduardo
Alonso, Daniel Fernando
author2_role author
author
author
author
dc.subject.none.fl_str_mv Breast Cancer
Metastasis
Surgery
Treatment
Vasopressin Analogue
topic Breast Cancer
Metastasis
Surgery
Treatment
Vasopressin Analogue
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background and Objectives: Desmopressin (DDAVP) is a synthetic derivative of vasopressin with hemostatic and fibrinolytic properties that has been used during surgery in patients with bleeding disorders. Our aim was to investigate the effect of DDAVP on lung and lymph node metastatic cell colonization using a preclinical mouse mammary carcinoma model of subcutaneous tumor manipulation and surgical excision. Methods: Female BALB/c mice bearing the highly aggressive F3II mammary carcinoma were subjected to repeated manipulations of primary tumors (0.5 kg/cm2 during 2 min), followed (or not) by surgical excision. DDAVP was administered intravenously 30 min before and 24 h after each manipulation or surgery, at a dose of 2 μg/kg. At the end of the experiment, mice were sacrificed and necropsied. Results: Tumor manipulation induced dissemination to the axillary nodes and increased up to 6‐fold the number of metastatic lung nodules. Perioperative treatment with DDAVP dramatically reduced regional metastasis. The incidence of lymph node involvement in manipulated animals was 12% with DDAVP and 87% without treatment (P < 0.02). Histopathological analysis of axillary nodes from DDAVP‐treated animals showed sinusal histiocytosis and no evidence of cancer cells. Metastatic lung nodules were also reduced about 65% in animals treated with DDAVP (P = 0.026). Conclusions: Our results suggest a potential clinical application of DDAVP in the management of breast cancer, as well as other aggressive solid tumors. DDAVP may be useful to reduce the risk of metastatic cell colonization both during and after surgical manipulation.
Fil: Giron, Santiago. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina
Fil: Tejera, Agueda M.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina
Fil: Ripoll, Giselle Vanina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background and Objectives: Desmopressin (DDAVP) is a synthetic derivative of vasopressin with hemostatic and fibrinolytic properties that has been used during surgery in patients with bleeding disorders. Our aim was to investigate the effect of DDAVP on lung and lymph node metastatic cell colonization using a preclinical mouse mammary carcinoma model of subcutaneous tumor manipulation and surgical excision. Methods: Female BALB/c mice bearing the highly aggressive F3II mammary carcinoma were subjected to repeated manipulations of primary tumors (0.5 kg/cm2 during 2 min), followed (or not) by surgical excision. DDAVP was administered intravenously 30 min before and 24 h after each manipulation or surgery, at a dose of 2 μg/kg. At the end of the experiment, mice were sacrificed and necropsied. Results: Tumor manipulation induced dissemination to the axillary nodes and increased up to 6‐fold the number of metastatic lung nodules. Perioperative treatment with DDAVP dramatically reduced regional metastasis. The incidence of lymph node involvement in manipulated animals was 12% with DDAVP and 87% without treatment (P < 0.02). Histopathological analysis of axillary nodes from DDAVP‐treated animals showed sinusal histiocytosis and no evidence of cancer cells. Metastatic lung nodules were also reduced about 65% in animals treated with DDAVP (P = 0.026). Conclusions: Our results suggest a potential clinical application of DDAVP in the management of breast cancer, as well as other aggressive solid tumors. DDAVP may be useful to reduce the risk of metastatic cell colonization both during and after surgical manipulation.
publishDate 2002
dc.date.none.fl_str_mv 2002-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43483
Giron, Santiago; Tejera, Agueda M.; Ripoll, Giselle Vanina; Gomez, Daniel Eduardo; Alonso, Daniel Fernando; Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation; Wiley-liss, Div John Wiley & Sons Inc; Journal of Surgical Oncology; 81; 1; 9-2002; 38-44
0022-4790
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43483
identifier_str_mv Giron, Santiago; Tejera, Agueda M.; Ripoll, Giselle Vanina; Gomez, Daniel Eduardo; Alonso, Daniel Fernando; Desmopressin inhibits lung and lymph node metastasis in a mouse mammary carcinoma model of surgical manipulation; Wiley-liss, Div John Wiley & Sons Inc; Journal of Surgical Oncology; 81; 1; 9-2002; 38-44
0022-4790
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jso.10141
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jso.10141
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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