β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2
- Autores
- Furlán, César; Sterin, Leonor Josefina; Borda, Enri Santiago
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- CD40, a member of the tumour necrosis factor-α receptor family, is constitutively expressed by cells of haematopoietic and non-haematopoietic origin, including fibroblasts. Signalling through this receptor molecule regulates inflammatory mediator secretion by many cell types. The work has been performed in healthy subjects and the authors studied, by cellular culture, flow cytometric analysis and ELISA assay, the expression of CD40 and PGE2 (prostaglandin E2) generation on gingival fibroblasts stimulated by β-AR (β-adrenoceptor) agonists. Herein, the authors demonstrate that β-AR subtype activation via their own specific agonists markedly increased CD40 expression on human gingival fibroblasts. This effect was prevented by β-AR subtype-specific antagonists. In addition, gingival fibroblast β-AR stimulation resulted in an increase in PGE2 generation. The inhibition of PLA2 (phospholipase A2) and COX-1 (cyclo-oxygenase-1) but not COX-2 impaired β-AR increase of PGE2, an effect that was restored by the addition of low concentrations of PGE2, suggesting that PGE2 generation is implicated in the mechanism underlying β-AR-agonist-mediated CD40 overexpression. Our work has revealed an endogenous β-AR mediator network involving gingival fibroblasts.
Fil: Furlán, César. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Sterin, Leonor Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina - Materia
-
Pge2
Cd40
Gingival Fibroblasts
Beta Adrenergic Receptor - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/14157
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β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2Furlán, CésarSterin, Leonor JosefinaBorda, Enri SantiagoPge2Cd40Gingival FibroblastsBeta Adrenergic Receptorhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3CD40, a member of the tumour necrosis factor-α receptor family, is constitutively expressed by cells of haematopoietic and non-haematopoietic origin, including fibroblasts. Signalling through this receptor molecule regulates inflammatory mediator secretion by many cell types. The work has been performed in healthy subjects and the authors studied, by cellular culture, flow cytometric analysis and ELISA assay, the expression of CD40 and PGE2 (prostaglandin E2) generation on gingival fibroblasts stimulated by β-AR (β-adrenoceptor) agonists. Herein, the authors demonstrate that β-AR subtype activation via their own specific agonists markedly increased CD40 expression on human gingival fibroblasts. This effect was prevented by β-AR subtype-specific antagonists. In addition, gingival fibroblast β-AR stimulation resulted in an increase in PGE2 generation. The inhibition of PLA2 (phospholipase A2) and COX-1 (cyclo-oxygenase-1) but not COX-2 impaired β-AR increase of PGE2, an effect that was restored by the addition of low concentrations of PGE2, suggesting that PGE2 generation is implicated in the mechanism underlying β-AR-agonist-mediated CD40 overexpression. Our work has revealed an endogenous β-AR mediator network involving gingival fibroblasts.Fil: Furlán, César. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Sterin, Leonor Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaWiley2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14157Furlán, César; Sterin, Leonor Josefina; Borda, Enri Santiago; β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2; Wiley; Cell Biology International; 34; 4; 4-2010; 365-3721065-69951095-8355enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1042/CBI20090028/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1042/CBI20090028info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:25Zoai:ri.conicet.gov.ar:11336/14157instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:26.155CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2 |
title |
β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2 |
spellingShingle |
β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2 Furlán, César Pge2 Cd40 Gingival Fibroblasts Beta Adrenergic Receptor |
title_short |
β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2 |
title_full |
β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2 |
title_fullStr |
β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2 |
title_full_unstemmed |
β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2 |
title_sort |
β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2 |
dc.creator.none.fl_str_mv |
Furlán, César Sterin, Leonor Josefina Borda, Enri Santiago |
author |
Furlán, César |
author_facet |
Furlán, César Sterin, Leonor Josefina Borda, Enri Santiago |
author_role |
author |
author2 |
Sterin, Leonor Josefina Borda, Enri Santiago |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Pge2 Cd40 Gingival Fibroblasts Beta Adrenergic Receptor |
topic |
Pge2 Cd40 Gingival Fibroblasts Beta Adrenergic Receptor |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
CD40, a member of the tumour necrosis factor-α receptor family, is constitutively expressed by cells of haematopoietic and non-haematopoietic origin, including fibroblasts. Signalling through this receptor molecule regulates inflammatory mediator secretion by many cell types. The work has been performed in healthy subjects and the authors studied, by cellular culture, flow cytometric analysis and ELISA assay, the expression of CD40 and PGE2 (prostaglandin E2) generation on gingival fibroblasts stimulated by β-AR (β-adrenoceptor) agonists. Herein, the authors demonstrate that β-AR subtype activation via their own specific agonists markedly increased CD40 expression on human gingival fibroblasts. This effect was prevented by β-AR subtype-specific antagonists. In addition, gingival fibroblast β-AR stimulation resulted in an increase in PGE2 generation. The inhibition of PLA2 (phospholipase A2) and COX-1 (cyclo-oxygenase-1) but not COX-2 impaired β-AR increase of PGE2, an effect that was restored by the addition of low concentrations of PGE2, suggesting that PGE2 generation is implicated in the mechanism underlying β-AR-agonist-mediated CD40 overexpression. Our work has revealed an endogenous β-AR mediator network involving gingival fibroblasts. Fil: Furlán, César. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina Fil: Sterin, Leonor Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina |
description |
CD40, a member of the tumour necrosis factor-α receptor family, is constitutively expressed by cells of haematopoietic and non-haematopoietic origin, including fibroblasts. Signalling through this receptor molecule regulates inflammatory mediator secretion by many cell types. The work has been performed in healthy subjects and the authors studied, by cellular culture, flow cytometric analysis and ELISA assay, the expression of CD40 and PGE2 (prostaglandin E2) generation on gingival fibroblasts stimulated by β-AR (β-adrenoceptor) agonists. Herein, the authors demonstrate that β-AR subtype activation via their own specific agonists markedly increased CD40 expression on human gingival fibroblasts. This effect was prevented by β-AR subtype-specific antagonists. In addition, gingival fibroblast β-AR stimulation resulted in an increase in PGE2 generation. The inhibition of PLA2 (phospholipase A2) and COX-1 (cyclo-oxygenase-1) but not COX-2 impaired β-AR increase of PGE2, an effect that was restored by the addition of low concentrations of PGE2, suggesting that PGE2 generation is implicated in the mechanism underlying β-AR-agonist-mediated CD40 overexpression. Our work has revealed an endogenous β-AR mediator network involving gingival fibroblasts. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/14157 Furlán, César; Sterin, Leonor Josefina; Borda, Enri Santiago; β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2; Wiley; Cell Biology International; 34; 4; 4-2010; 365-372 1065-6995 1095-8355 |
url |
http://hdl.handle.net/11336/14157 |
identifier_str_mv |
Furlán, César; Sterin, Leonor Josefina; Borda, Enri Santiago; β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2; Wiley; Cell Biology International; 34; 4; 4-2010; 365-372 1065-6995 1095-8355 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1042/CBI20090028/abstract info:eu-repo/semantics/altIdentifier/doi/10.1042/CBI20090028 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613103580348416 |
score |
13.070432 |