β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2

Autores
Furlán, César; Sterin, Leonor Josefina; Borda, Enri Santiago
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
CD40, a member of the tumour necrosis factor-α receptor family, is constitutively expressed by cells of haematopoietic and non-haematopoietic origin, including fibroblasts. Signalling through this receptor molecule regulates inflammatory mediator secretion by many cell types. The work has been performed in healthy subjects and the authors studied, by cellular culture, flow cytometric analysis and ELISA assay, the expression of CD40 and PGE2 (prostaglandin E2) generation on gingival fibroblasts stimulated by β-AR (β-adrenoceptor) agonists. Herein, the authors demonstrate that β-AR subtype activation via their own specific agonists markedly increased CD40 expression on human gingival fibroblasts. This effect was prevented by β-AR subtype-specific antagonists. In addition, gingival fibroblast β-AR stimulation resulted in an increase in PGE2 generation. The inhibition of PLA2 (phospholipase A2) and COX-1 (cyclo-oxygenase-1) but not COX-2 impaired β-AR increase of PGE2, an effect that was restored by the addition of low concentrations of PGE2, suggesting that PGE2 generation is implicated in the mechanism underlying β-AR-agonist-mediated CD40 overexpression. Our work has revealed an endogenous β-AR mediator network involving gingival fibroblasts.
Fil: Furlán, César. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Sterin, Leonor Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Materia
Pge2
Cd40
Gingival Fibroblasts
Beta Adrenergic Receptor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14157
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2Furlán, CésarSterin, Leonor JosefinaBorda, Enri SantiagoPge2Cd40Gingival FibroblastsBeta Adrenergic Receptorhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3CD40, a member of the tumour necrosis factor-α receptor family, is constitutively expressed by cells of haematopoietic and non-haematopoietic origin, including fibroblasts. Signalling through this receptor molecule regulates inflammatory mediator secretion by many cell types. The work has been performed in healthy subjects and the authors studied, by cellular culture, flow cytometric analysis and ELISA assay, the expression of CD40 and PGE2 (prostaglandin E2) generation on gingival fibroblasts stimulated by β-AR (β-adrenoceptor) agonists. Herein, the authors demonstrate that β-AR subtype activation via their own specific agonists markedly increased CD40 expression on human gingival fibroblasts. This effect was prevented by β-AR subtype-specific antagonists. In addition, gingival fibroblast β-AR stimulation resulted in an increase in PGE2 generation. The inhibition of PLA2 (phospholipase A2) and COX-1 (cyclo-oxygenase-1) but not COX-2 impaired β-AR increase of PGE2, an effect that was restored by the addition of low concentrations of PGE2, suggesting that PGE2 generation is implicated in the mechanism underlying β-AR-agonist-mediated CD40 overexpression. Our work has revealed an endogenous β-AR mediator network involving gingival fibroblasts.Fil: Furlán, César. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Sterin, Leonor Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaWiley2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14157Furlán, César; Sterin, Leonor Josefina; Borda, Enri Santiago; β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2; Wiley; Cell Biology International; 34; 4; 4-2010; 365-3721065-69951095-8355enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1042/CBI20090028/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1042/CBI20090028info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:03:29Zoai:ri.conicet.gov.ar:11336/14157instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:03:29.285CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2
title β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2
spellingShingle β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2
Furlán, César
Pge2
Cd40
Gingival Fibroblasts
Beta Adrenergic Receptor
title_short β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2
title_full β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2
title_fullStr β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2
title_full_unstemmed β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2
title_sort β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2
dc.creator.none.fl_str_mv Furlán, César
Sterin, Leonor Josefina
Borda, Enri Santiago
author Furlán, César
author_facet Furlán, César
Sterin, Leonor Josefina
Borda, Enri Santiago
author_role author
author2 Sterin, Leonor Josefina
Borda, Enri Santiago
author2_role author
author
dc.subject.none.fl_str_mv Pge2
Cd40
Gingival Fibroblasts
Beta Adrenergic Receptor
topic Pge2
Cd40
Gingival Fibroblasts
Beta Adrenergic Receptor
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv CD40, a member of the tumour necrosis factor-α receptor family, is constitutively expressed by cells of haematopoietic and non-haematopoietic origin, including fibroblasts. Signalling through this receptor molecule regulates inflammatory mediator secretion by many cell types. The work has been performed in healthy subjects and the authors studied, by cellular culture, flow cytometric analysis and ELISA assay, the expression of CD40 and PGE2 (prostaglandin E2) generation on gingival fibroblasts stimulated by β-AR (β-adrenoceptor) agonists. Herein, the authors demonstrate that β-AR subtype activation via their own specific agonists markedly increased CD40 expression on human gingival fibroblasts. This effect was prevented by β-AR subtype-specific antagonists. In addition, gingival fibroblast β-AR stimulation resulted in an increase in PGE2 generation. The inhibition of PLA2 (phospholipase A2) and COX-1 (cyclo-oxygenase-1) but not COX-2 impaired β-AR increase of PGE2, an effect that was restored by the addition of low concentrations of PGE2, suggesting that PGE2 generation is implicated in the mechanism underlying β-AR-agonist-mediated CD40 overexpression. Our work has revealed an endogenous β-AR mediator network involving gingival fibroblasts.
Fil: Furlán, César. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Sterin, Leonor Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
description CD40, a member of the tumour necrosis factor-α receptor family, is constitutively expressed by cells of haematopoietic and non-haematopoietic origin, including fibroblasts. Signalling through this receptor molecule regulates inflammatory mediator secretion by many cell types. The work has been performed in healthy subjects and the authors studied, by cellular culture, flow cytometric analysis and ELISA assay, the expression of CD40 and PGE2 (prostaglandin E2) generation on gingival fibroblasts stimulated by β-AR (β-adrenoceptor) agonists. Herein, the authors demonstrate that β-AR subtype activation via their own specific agonists markedly increased CD40 expression on human gingival fibroblasts. This effect was prevented by β-AR subtype-specific antagonists. In addition, gingival fibroblast β-AR stimulation resulted in an increase in PGE2 generation. The inhibition of PLA2 (phospholipase A2) and COX-1 (cyclo-oxygenase-1) but not COX-2 impaired β-AR increase of PGE2, an effect that was restored by the addition of low concentrations of PGE2, suggesting that PGE2 generation is implicated in the mechanism underlying β-AR-agonist-mediated CD40 overexpression. Our work has revealed an endogenous β-AR mediator network involving gingival fibroblasts.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14157
Furlán, César; Sterin, Leonor Josefina; Borda, Enri Santiago; β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2; Wiley; Cell Biology International; 34; 4; 4-2010; 365-372
1065-6995
1095-8355
url http://hdl.handle.net/11336/14157
identifier_str_mv Furlán, César; Sterin, Leonor Josefina; Borda, Enri Santiago; β-Adrenergic-induced CD40 overexpression on gingival fibroblasts: role of PGE2; Wiley; Cell Biology International; 34; 4; 4-2010; 365-372
1065-6995
1095-8355
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1042/CBI20090028/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1042/CBI20090028
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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