Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama)

Autores: Bianchi, Carolina Paula; Meikle, A.; Benavente, Micaela Andrea; Álvarez, M.; Trasorras, Virginia Luz; Miragaya, Marcelo; Rodriguez, E.; Aba, Marcelo Alfredo
Año de publicación: 2015
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Endometrial expression of oestrogen receptor-a (ERa),progesterone receptor (PR) and cyclooxigenase-2 (COX-2)was evaluated in non-pregnant and pregnant llamas during theperiod when luteolysis/maternal recognition of pregnancy isexpected to occur. Females (n = 28) were divided into twogroups: non-pregnant llamas were induced to ovulate with aBuserelin injection, and endometrial biopsies were obtained onday 8 (n = 5) or 12 (n = 5) post-induction of ovulation.Animals of the pregnant group (n = 18) were mated with afertile male. Pregnancy was confirmed by the visualization ofthe embryo collected by transcervical flushing in 5 of 9 animalson day 8 post-mating and by progesterone profile on day 12post-mating in 4 of 9 animals, when endometrial biopsies wereobtained. An immunohistochemical technique was used toevaluate receptors population and COX-2 expression.Pregnant llamas showed a higher percentage of positive cellsand stronger intensity for ERa than for non-pregnant llamasin stroma on day 8 and in the luminal epithelium on day 12post-induction of ovulation, while a deep decrease in endometrialPR population was reported in pregnant llamas on thatday in luminal and glandular epithelia and stroma. In theluminal epithelium, COX-2 expression was lower in pregnantthan in non-pregnant animals. Briefly, the increase of ERa inpregnant llamas gives further support to the hypothesis thatoestrogens are involved in the mechanism of maternal recognitionof pregnancy. Endometrial PR decrease in pregnantllamas might be a necessary event to allow the expression ofproteins involved in conceptus attachment, a mechanismwidely accepted in other species. Moreover, embryo seems toattenuate maternal PGF2a secretion during early pregnancy bydecreasing the endometrial expression of COX-2 in the luminalepithelium of pregnant llamas.
Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Meikle, A.. Universidad del Uruguay; Uruguay
Fil: Benavente, Micaela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Álvarez, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias. Departamento de Fisiopatologia. Laboratorio de Endocrinologia; Argentina
Fil: Trasorras, Virginia Luz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Rodriguez, E.. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias. Departamento de Fisiopatologia. Laboratorio de Endocrinologia; Argentina
Fil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Materia: Endometrium
Receptors
Early Pregnancy
Llamas
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/43639

Acceder

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repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama)Bianchi, Carolina PaulaMeikle, A.Benavente, Micaela AndreaÁlvarez, M.Trasorras, Virginia LuzMiragaya, MarceloRodriguez, E.Aba, Marcelo AlfredoEndometriumReceptorsEarly PregnancyLlamashttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Endometrial expression of oestrogen receptor-a (ERa),progesterone receptor (PR) and cyclooxigenase-2 (COX-2)was evaluated in non-pregnant and pregnant llamas during theperiod when luteolysis/maternal recognition of pregnancy isexpected to occur. Females (n = 28) were divided into twogroups: non-pregnant llamas were induced to ovulate with aBuserelin injection, and endometrial biopsies were obtained onday 8 (n = 5) or 12 (n = 5) post-induction of ovulation.Animals of the pregnant group (n = 18) were mated with afertile male. Pregnancy was confirmed by the visualization ofthe embryo collected by transcervical flushing in 5 of 9 animalson day 8 post-mating and by progesterone profile on day 12post-mating in 4 of 9 animals, when endometrial biopsies wereobtained. An immunohistochemical technique was used toevaluate receptors population and COX-2 expression.Pregnant llamas showed a higher percentage of positive cellsand stronger intensity for ERa than for non-pregnant llamasin stroma on day 8 and in the luminal epithelium on day 12post-induction of ovulation, while a deep decrease in endometrialPR population was reported in pregnant llamas on thatday in luminal and glandular epithelia and stroma. In theluminal epithelium, COX-2 expression was lower in pregnantthan in non-pregnant animals. Briefly, the increase of ERa inpregnant llamas gives further support to the hypothesis thatoestrogens are involved in the mechanism of maternal recognitionof pregnancy. Endometrial PR decrease in pregnantllamas might be a necessary event to allow the expression ofproteins involved in conceptus attachment, a mechanismwidely accepted in other species. Moreover, embryo seems toattenuate maternal PGF2a secretion during early pregnancy bydecreasing the endometrial expression of COX-2 in the luminalepithelium of pregnant llamas.Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Meikle, A.. Universidad del Uruguay; UruguayFil: Benavente, Micaela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Álvarez, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias. Departamento de Fisiopatologia. Laboratorio de Endocrinologia; ArgentinaFil: Trasorras, Virginia Luz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Rodriguez, E.. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias. Departamento de Fisiopatologia. Laboratorio de Endocrinologia; ArgentinaFil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaWiley Blackwell Publishing, Inc2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43639Bianchi, Carolina Paula; Meikle, A.; Benavente, Micaela Andrea; Álvarez, M.; Trasorras, Virginia Luz; et al.; Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama); Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 6; 12-2015; 980-9880936-6768CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/pdf/10.1111/rda.12618info:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12618info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:35Zoai:ri.conicet.gov.ar:11336/43639instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:35.508CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama)
title	Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama)
spellingShingle	Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama) Bianchi, Carolina Paula Endometrium Receptors Early Pregnancy Llamas
title_short	Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama)
title_full	Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama)
title_fullStr	Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama)
title_full_unstemmed	Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama)
title_sort	Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama)
dc.creator.none.fl_str_mv	Bianchi, Carolina Paula Meikle, A. Benavente, Micaela Andrea Álvarez, M. Trasorras, Virginia Luz Miragaya, Marcelo Rodriguez, E. Aba, Marcelo Alfredo
author	Bianchi, Carolina Paula
author_facet	Bianchi, Carolina Paula Meikle, A. Benavente, Micaela Andrea Álvarez, M. Trasorras, Virginia Luz Miragaya, Marcelo Rodriguez, E. Aba, Marcelo Alfredo
author_role	author
author2	Meikle, A. Benavente, Micaela Andrea Álvarez, M. Trasorras, Virginia Luz Miragaya, Marcelo Rodriguez, E. Aba, Marcelo Alfredo
author2_role	author author author author author author author
dc.subject.none.fl_str_mv	Endometrium Receptors Early Pregnancy Llamas
topic	Endometrium Receptors Early Pregnancy Llamas
purl_subject.fl_str_mv	https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv	Endometrial expression of oestrogen receptor-a (ERa),progesterone receptor (PR) and cyclooxigenase-2 (COX-2)was evaluated in non-pregnant and pregnant llamas during theperiod when luteolysis/maternal recognition of pregnancy isexpected to occur. Females (n = 28) were divided into twogroups: non-pregnant llamas were induced to ovulate with aBuserelin injection, and endometrial biopsies were obtained onday 8 (n = 5) or 12 (n = 5) post-induction of ovulation.Animals of the pregnant group (n = 18) were mated with afertile male. Pregnancy was confirmed by the visualization ofthe embryo collected by transcervical flushing in 5 of 9 animalson day 8 post-mating and by progesterone profile on day 12post-mating in 4 of 9 animals, when endometrial biopsies wereobtained. An immunohistochemical technique was used toevaluate receptors population and COX-2 expression.Pregnant llamas showed a higher percentage of positive cellsand stronger intensity for ERa than for non-pregnant llamasin stroma on day 8 and in the luminal epithelium on day 12post-induction of ovulation, while a deep decrease in endometrialPR population was reported in pregnant llamas on thatday in luminal and glandular epithelia and stroma. In theluminal epithelium, COX-2 expression was lower in pregnantthan in non-pregnant animals. Briefly, the increase of ERa inpregnant llamas gives further support to the hypothesis thatoestrogens are involved in the mechanism of maternal recognitionof pregnancy. Endometrial PR decrease in pregnantllamas might be a necessary event to allow the expression ofproteins involved in conceptus attachment, a mechanismwidely accepted in other species. Moreover, embryo seems toattenuate maternal PGF2a secretion during early pregnancy bydecreasing the endometrial expression of COX-2 in the luminalepithelium of pregnant llamas. Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Meikle, A.. Universidad del Uruguay; Uruguay Fil: Benavente, Micaela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Álvarez, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias. Departamento de Fisiopatologia. Laboratorio de Endocrinologia; Argentina Fil: Trasorras, Virginia Luz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina Fil: Rodriguez, E.. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias. Departamento de Fisiopatologia. Laboratorio de Endocrinologia; Argentina Fil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
description	Endometrial expression of oestrogen receptor-a (ERa),progesterone receptor (PR) and cyclooxigenase-2 (COX-2)was evaluated in non-pregnant and pregnant llamas during theperiod when luteolysis/maternal recognition of pregnancy isexpected to occur. Females (n = 28) were divided into twogroups: non-pregnant llamas were induced to ovulate with aBuserelin injection, and endometrial biopsies were obtained onday 8 (n = 5) or 12 (n = 5) post-induction of ovulation.Animals of the pregnant group (n = 18) were mated with afertile male. Pregnancy was confirmed by the visualization ofthe embryo collected by transcervical flushing in 5 of 9 animalson day 8 post-mating and by progesterone profile on day 12post-mating in 4 of 9 animals, when endometrial biopsies wereobtained. An immunohistochemical technique was used toevaluate receptors population and COX-2 expression.Pregnant llamas showed a higher percentage of positive cellsand stronger intensity for ERa than for non-pregnant llamasin stroma on day 8 and in the luminal epithelium on day 12post-induction of ovulation, while a deep decrease in endometrialPR population was reported in pregnant llamas on thatday in luminal and glandular epithelia and stroma. In theluminal epithelium, COX-2 expression was lower in pregnantthan in non-pregnant animals. Briefly, the increase of ERa inpregnant llamas gives further support to the hypothesis thatoestrogens are involved in the mechanism of maternal recognitionof pregnancy. Endometrial PR decrease in pregnantllamas might be a necessary event to allow the expression ofproteins involved in conceptus attachment, a mechanismwidely accepted in other species. Moreover, embryo seems toattenuate maternal PGF2a secretion during early pregnancy bydecreasing the endometrial expression of COX-2 in the luminalepithelium of pregnant llamas.
publishDate	2015
dc.date.none.fl_str_mv	2015-12
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/43639 Bianchi, Carolina Paula; Meikle, A.; Benavente, Micaela Andrea; Álvarez, M.; Trasorras, Virginia Luz; et al.; Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama); Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 6; 12-2015; 980-988 0936-6768 CONICET Digital CONICET
url	http://hdl.handle.net/11336/43639
identifier_str_mv	Bianchi, Carolina Paula; Meikle, A.; Benavente, Micaela Andrea; Álvarez, M.; Trasorras, Virginia Luz; et al.; Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama); Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 6; 12-2015; 980-988 0936-6768 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/pdf/10.1111/rda.12618 info:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12618
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv	Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Estrogen and Progesterone Receptors and COX-2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama)

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