Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss

Autores
Ahmadian Chashmi, Najmeh; Sharifi, Mozafar; Yousefzadi, Morteza; Behmanesh, Mehrdad; Rezadoost, Hassan; Cardillo, Alejandra Beatriz; Palazon, Javier
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Linum album is a herbaceous medicinal plant that contains some lignans with antiviral and anticancer properties such as podophyllotoxin (PTOX) and 6-methoxy podophyllotoxin (MPTOX). In this research, hairy root cultures of L. album were established by transformation with Agrobacterium rhizogenes strains LBA9402, A4, AR15834 and A. tumefaciens strain C58C1 (pRiA4). The presence of PTOX and MPTOX in the hairy roots was verified by ESI/MS in positive ion mode. MPTOX was confirmed and its enantiomer determined by nuclear magnetic resonance spectroscopy and circular dichroism spectroscopy, respectively. PTOX and MPTOX production was determined by HPLC, in different lines of hairy roots. The results showed that all obtained hairy root lines produced higher yield of lignans than mother plant roots. In addition, the lignans content in the roots derived from A. rhizogenes strain LBA9402 was higher than in those obtained from A. tumefaciens strain C58C1.
Fil: Ahmadian Chashmi, Najmeh. Tarbiat Modares University; Irán
Fil: Sharifi, Mozafar. Tarbiat Modares University; Irán
Fil: Yousefzadi, Morteza. Tarbiat Modares University; Irán
Fil: Behmanesh, Mehrdad. Tarbiat Modares University; Irán
Fil: Rezadoost, Hassan. Shahid Beheshti University; Irán
Fil: Cardillo, Alejandra Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Palazon, Javier. Universidad de Barcelona. Facultad de Farmacia; España;
Materia
6-METHOXY PODOPHYLLOTOXIN (MPTOX)
AGROBACTERIUM
HAIRY ROOTS
LINUM ALBUM
PODOPHYLLOTOXIN (PTOX)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1934
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1934
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex BoissAhmadian Chashmi, NajmehSharifi, MozafarYousefzadi, MortezaBehmanesh, MehrdadRezadoost, HassanCardillo, Alejandra BeatrizPalazon, Javier6-METHOXY PODOPHYLLOTOXIN (MPTOX)AGROBACTERIUMHAIRY ROOTSLINUM ALBUMPODOPHYLLOTOXIN (PTOX)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Linum album is a herbaceous medicinal plant that contains some lignans with antiviral and anticancer properties such as podophyllotoxin (PTOX) and 6-methoxy podophyllotoxin (MPTOX). In this research, hairy root cultures of L. album were established by transformation with Agrobacterium rhizogenes strains LBA9402, A4, AR15834 and A. tumefaciens strain C58C1 (pRiA4). The presence of PTOX and MPTOX in the hairy roots was verified by ESI/MS in positive ion mode. MPTOX was confirmed and its enantiomer determined by nuclear magnetic resonance spectroscopy and circular dichroism spectroscopy, respectively. PTOX and MPTOX production was determined by HPLC, in different lines of hairy roots. The results showed that all obtained hairy root lines produced higher yield of lignans than mother plant roots. In addition, the lignans content in the roots derived from A. rhizogenes strain LBA9402 was higher than in those obtained from A. tumefaciens strain C58C1.Fil: Ahmadian Chashmi, Najmeh. Tarbiat Modares University; IránFil: Sharifi, Mozafar. Tarbiat Modares University; IránFil: Yousefzadi, Morteza. Tarbiat Modares University; IránFil: Behmanesh, Mehrdad. Tarbiat Modares University; IránFil: Rezadoost, Hassan. Shahid Beheshti University; IránFil: Cardillo, Alejandra Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Palazon, Javier. Universidad de Barcelona. Facultad de Farmacia; España;Birkhauser Boston Inc2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1934Ahmadian Chashmi, Najmeh; Sharifi, Mozafar; Yousefzadi, Morteza; Behmanesh, Mehrdad; Rezadoost, Hassan; et al.; Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss; Birkhauser Boston Inc; Medicinal Chemistry Research; 22; 2; 2-2013; 745-7521054-25231554-8120enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00044-012-0067-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s00044-012-0067-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:00:59Zoai:ri.conicet.gov.ar:11336/1934instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:01:00.03CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss
title Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss
spellingShingle Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss
Ahmadian Chashmi, Najmeh
6-METHOXY PODOPHYLLOTOXIN (MPTOX)
AGROBACTERIUM
HAIRY ROOTS
LINUM ALBUM
PODOPHYLLOTOXIN (PTOX)
title_short Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss
title_full Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss
title_fullStr Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss
title_full_unstemmed Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss
title_sort Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss
dc.creator.none.fl_str_mv Ahmadian Chashmi, Najmeh
Sharifi, Mozafar
Yousefzadi, Morteza
Behmanesh, Mehrdad
Rezadoost, Hassan
Cardillo, Alejandra Beatriz
Palazon, Javier
author Ahmadian Chashmi, Najmeh
author_facet Ahmadian Chashmi, Najmeh
Sharifi, Mozafar
Yousefzadi, Morteza
Behmanesh, Mehrdad
Rezadoost, Hassan
Cardillo, Alejandra Beatriz
Palazon, Javier
author_role author
author2 Sharifi, Mozafar
Yousefzadi, Morteza
Behmanesh, Mehrdad
Rezadoost, Hassan
Cardillo, Alejandra Beatriz
Palazon, Javier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv 6-METHOXY PODOPHYLLOTOXIN (MPTOX)
AGROBACTERIUM
HAIRY ROOTS
LINUM ALBUM
PODOPHYLLOTOXIN (PTOX)
topic 6-METHOXY PODOPHYLLOTOXIN (MPTOX)
AGROBACTERIUM
HAIRY ROOTS
LINUM ALBUM
PODOPHYLLOTOXIN (PTOX)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Linum album is a herbaceous medicinal plant that contains some lignans with antiviral and anticancer properties such as podophyllotoxin (PTOX) and 6-methoxy podophyllotoxin (MPTOX). In this research, hairy root cultures of L. album were established by transformation with Agrobacterium rhizogenes strains LBA9402, A4, AR15834 and A. tumefaciens strain C58C1 (pRiA4). The presence of PTOX and MPTOX in the hairy roots was verified by ESI/MS in positive ion mode. MPTOX was confirmed and its enantiomer determined by nuclear magnetic resonance spectroscopy and circular dichroism spectroscopy, respectively. PTOX and MPTOX production was determined by HPLC, in different lines of hairy roots. The results showed that all obtained hairy root lines produced higher yield of lignans than mother plant roots. In addition, the lignans content in the roots derived from A. rhizogenes strain LBA9402 was higher than in those obtained from A. tumefaciens strain C58C1.
Fil: Ahmadian Chashmi, Najmeh. Tarbiat Modares University; Irán
Fil: Sharifi, Mozafar. Tarbiat Modares University; Irán
Fil: Yousefzadi, Morteza. Tarbiat Modares University; Irán
Fil: Behmanesh, Mehrdad. Tarbiat Modares University; Irán
Fil: Rezadoost, Hassan. Shahid Beheshti University; Irán
Fil: Cardillo, Alejandra Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Palazon, Javier. Universidad de Barcelona. Facultad de Farmacia; España;
description Linum album is a herbaceous medicinal plant that contains some lignans with antiviral and anticancer properties such as podophyllotoxin (PTOX) and 6-methoxy podophyllotoxin (MPTOX). In this research, hairy root cultures of L. album were established by transformation with Agrobacterium rhizogenes strains LBA9402, A4, AR15834 and A. tumefaciens strain C58C1 (pRiA4). The presence of PTOX and MPTOX in the hairy roots was verified by ESI/MS in positive ion mode. MPTOX was confirmed and its enantiomer determined by nuclear magnetic resonance spectroscopy and circular dichroism spectroscopy, respectively. PTOX and MPTOX production was determined by HPLC, in different lines of hairy roots. The results showed that all obtained hairy root lines produced higher yield of lignans than mother plant roots. In addition, the lignans content in the roots derived from A. rhizogenes strain LBA9402 was higher than in those obtained from A. tumefaciens strain C58C1.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1934
Ahmadian Chashmi, Najmeh; Sharifi, Mozafar; Yousefzadi, Morteza; Behmanesh, Mehrdad; Rezadoost, Hassan; et al.; Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss; Birkhauser Boston Inc; Medicinal Chemistry Research; 22; 2; 2-2013; 745-752
1054-2523
1554-8120
url http://hdl.handle.net/11336/1934
identifier_str_mv Ahmadian Chashmi, Najmeh; Sharifi, Mozafar; Yousefzadi, Morteza; Behmanesh, Mehrdad; Rezadoost, Hassan; et al.; Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss; Birkhauser Boston Inc; Medicinal Chemistry Research; 22; 2; 2-2013; 745-752
1054-2523
1554-8120
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00044-012-0067-1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00044-012-0067-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Birkhauser Boston Inc
publisher.none.fl_str_mv Birkhauser Boston Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781183304663040
score 12.928904

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