Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines

Autores
Calderón, Pablo Luciano Sebastian; Mauri, Nuria; Muñoz, Claudio Javier; Carbonell Bejerano, Pablo; Bree, Laura; Sola, Cristobal; Gómez Talquenca, Sebastián; Royo, Carolina; Ibañez, Javier; Martinez-Zapater, José Miguel; Lijavetzky, Diego Claudio
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Grapevine (Vitis vinifera L.) cultivars are clonally propagated to preserve their varietal 26 attributes. However, novel genetic variation still accumulates due to somatic mutations. Aiming 27 to study the potential impact of clonal propagation history on grapevines intra-cultivar genetic 28 diversity, we have focused on ‘Malbec’. This cultivar is appreciated for red wines elaboration, 29 it was originated in Southwestern France and introduced into Argentina during the 1850s. Here, 30 we generated whole-genome resequencing data for four ‘Malbec’ clones with different 31 historical backgrounds. A stringent variant calling procedure was established to identify reliable 32 clonal polymorphisms, additionally corroborated by Sanger sequencing. This analysis retrieved 33 941 single nucleotide variants (SNVs), occurring among the analyzed clones. Based on a set of 34 validated SNVs, a genotyping experiment was custom-designed to survey ‘Malbec’ genetic 35 diversity. We successfully genotyped 214 samples and identified 14 different clonal genotypes, 36 that clustered into two genetically divergent groups. Group-Ar was driven by clones with a long 37 history of clonal propagation in Argentina, while Group-Fr was driven by clones that have 38 longer remained in Europe. Findings show the ability of such approaches for clonal genotypes 39 identification in grapevines. In particular, we provide evidence on how human actions may have 40 shaped ‘Malbec’ extant genetic diversity pattern.
Fil: Calderón, Pablo Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Mauri, Nuria. Consejo Superior de Investigaciones Científicas; España
Fil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Carbonell Bejerano, Pablo. Max Planck Institute for Biology of Ageing; Alemania
Fil: Bree, Laura. No especifíca;
Fil: Sola, Cristobal. No especifíca;
Fil: Gómez Talquenca, Sebastián. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Royo, Carolina. Consejo Superior de Investigaciones Científicas; España
Fil: Ibañez, Javier. Consejo Superior de Investigaciones Científicas; España
Fil: Martinez-Zapater, José Miguel. Consejo Superior de Investigaciones Científicas; España
Fil: Lijavetzky, Diego Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
MALBEC
GENOMA
CLONAL DIVERSITY
SOMATIC VARIATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/138748
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/138748
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevinesCalderón, Pablo Luciano SebastianMauri, NuriaMuñoz, Claudio JavierCarbonell Bejerano, PabloBree, LauraSola, CristobalGómez Talquenca, SebastiánRoyo, CarolinaIbañez, JavierMartinez-Zapater, José MiguelLijavetzky, Diego ClaudioMALBECGENOMACLONAL DIVERSITYSOMATIC VARIATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Grapevine (Vitis vinifera L.) cultivars are clonally propagated to preserve their varietal 26 attributes. However, novel genetic variation still accumulates due to somatic mutations. Aiming 27 to study the potential impact of clonal propagation history on grapevines intra-cultivar genetic 28 diversity, we have focused on ‘Malbec’. This cultivar is appreciated for red wines elaboration, 29 it was originated in Southwestern France and introduced into Argentina during the 1850s. Here, 30 we generated whole-genome resequencing data for four ‘Malbec’ clones with different 31 historical backgrounds. A stringent variant calling procedure was established to identify reliable 32 clonal polymorphisms, additionally corroborated by Sanger sequencing. This analysis retrieved 33 941 single nucleotide variants (SNVs), occurring among the analyzed clones. Based on a set of 34 validated SNVs, a genotyping experiment was custom-designed to survey ‘Malbec’ genetic 35 diversity. We successfully genotyped 214 samples and identified 14 different clonal genotypes, 36 that clustered into two genetically divergent groups. Group-Ar was driven by clones with a long 37 history of clonal propagation in Argentina, while Group-Fr was driven by clones that have 38 longer remained in Europe. Findings show the ability of such approaches for clonal genotypes 39 identification in grapevines. In particular, we provide evidence on how human actions may have 40 shaped ‘Malbec’ extant genetic diversity pattern.Fil: Calderón, Pablo Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Mauri, Nuria. Consejo Superior de Investigaciones Científicas; EspañaFil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Carbonell Bejerano, Pablo. Max Planck Institute for Biology of Ageing; AlemaniaFil: Bree, Laura. No especifíca;Fil: Sola, Cristobal. No especifíca;Fil: Gómez Talquenca, Sebastián. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Royo, Carolina. Consejo Superior de Investigaciones Científicas; EspañaFil: Ibañez, Javier. Consejo Superior de Investigaciones Científicas; EspañaFil: Martinez-Zapater, José Miguel. Consejo Superior de Investigaciones Científicas; EspañaFil: Lijavetzky, Diego Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaCold Spring Harbor Laboratory Press2020-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138748Calderón, Pablo Luciano Sebastian; Mauri, Nuria; Muñoz, Claudio Javier; Carbonell Bejerano, Pablo; Bree, Laura; et al.; Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines; Cold Spring Harbor Laboratory Press; bioRxiv; 10-2020; 1-202692-8205CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1101/2020.10.27.356790info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2020.10.27.356790v1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:29:45Zoai:ri.conicet.gov.ar:11336/138748instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:29:45.476CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines
title Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines
spellingShingle Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines
Calderón, Pablo Luciano Sebastian
MALBEC
GENOMA
CLONAL DIVERSITY
SOMATIC VARIATION
title_short Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines
title_full Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines
title_fullStr Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines
title_full_unstemmed Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines
title_sort Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines
dc.creator.none.fl_str_mv Calderón, Pablo Luciano Sebastian
Mauri, Nuria
Muñoz, Claudio Javier
Carbonell Bejerano, Pablo
Bree, Laura
Sola, Cristobal
Gómez Talquenca, Sebastián
Royo, Carolina
Ibañez, Javier
Martinez-Zapater, José Miguel
Lijavetzky, Diego Claudio
author Calderón, Pablo Luciano Sebastian
author_facet Calderón, Pablo Luciano Sebastian
Mauri, Nuria
Muñoz, Claudio Javier
Carbonell Bejerano, Pablo
Bree, Laura
Sola, Cristobal
Gómez Talquenca, Sebastián
Royo, Carolina
Ibañez, Javier
Martinez-Zapater, José Miguel
Lijavetzky, Diego Claudio
author_role author
author2 Mauri, Nuria
Muñoz, Claudio Javier
Carbonell Bejerano, Pablo
Bree, Laura
Sola, Cristobal
Gómez Talquenca, Sebastián
Royo, Carolina
Ibañez, Javier
Martinez-Zapater, José Miguel
Lijavetzky, Diego Claudio
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MALBEC
GENOMA
CLONAL DIVERSITY
SOMATIC VARIATION
topic MALBEC
GENOMA
CLONAL DIVERSITY
SOMATIC VARIATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Grapevine (Vitis vinifera L.) cultivars are clonally propagated to preserve their varietal 26 attributes. However, novel genetic variation still accumulates due to somatic mutations. Aiming 27 to study the potential impact of clonal propagation history on grapevines intra-cultivar genetic 28 diversity, we have focused on ‘Malbec’. This cultivar is appreciated for red wines elaboration, 29 it was originated in Southwestern France and introduced into Argentina during the 1850s. Here, 30 we generated whole-genome resequencing data for four ‘Malbec’ clones with different 31 historical backgrounds. A stringent variant calling procedure was established to identify reliable 32 clonal polymorphisms, additionally corroborated by Sanger sequencing. This analysis retrieved 33 941 single nucleotide variants (SNVs), occurring among the analyzed clones. Based on a set of 34 validated SNVs, a genotyping experiment was custom-designed to survey ‘Malbec’ genetic 35 diversity. We successfully genotyped 214 samples and identified 14 different clonal genotypes, 36 that clustered into two genetically divergent groups. Group-Ar was driven by clones with a long 37 history of clonal propagation in Argentina, while Group-Fr was driven by clones that have 38 longer remained in Europe. Findings show the ability of such approaches for clonal genotypes 39 identification in grapevines. In particular, we provide evidence on how human actions may have 40 shaped ‘Malbec’ extant genetic diversity pattern.
Fil: Calderón, Pablo Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Mauri, Nuria. Consejo Superior de Investigaciones Científicas; España
Fil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Carbonell Bejerano, Pablo. Max Planck Institute for Biology of Ageing; Alemania
Fil: Bree, Laura. No especifíca;
Fil: Sola, Cristobal. No especifíca;
Fil: Gómez Talquenca, Sebastián. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Royo, Carolina. Consejo Superior de Investigaciones Científicas; España
Fil: Ibañez, Javier. Consejo Superior de Investigaciones Científicas; España
Fil: Martinez-Zapater, José Miguel. Consejo Superior de Investigaciones Científicas; España
Fil: Lijavetzky, Diego Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description Grapevine (Vitis vinifera L.) cultivars are clonally propagated to preserve their varietal 26 attributes. However, novel genetic variation still accumulates due to somatic mutations. Aiming 27 to study the potential impact of clonal propagation history on grapevines intra-cultivar genetic 28 diversity, we have focused on ‘Malbec’. This cultivar is appreciated for red wines elaboration, 29 it was originated in Southwestern France and introduced into Argentina during the 1850s. Here, 30 we generated whole-genome resequencing data for four ‘Malbec’ clones with different 31 historical backgrounds. A stringent variant calling procedure was established to identify reliable 32 clonal polymorphisms, additionally corroborated by Sanger sequencing. This analysis retrieved 33 941 single nucleotide variants (SNVs), occurring among the analyzed clones. Based on a set of 34 validated SNVs, a genotyping experiment was custom-designed to survey ‘Malbec’ genetic 35 diversity. We successfully genotyped 214 samples and identified 14 different clonal genotypes, 36 that clustered into two genetically divergent groups. Group-Ar was driven by clones with a long 37 history of clonal propagation in Argentina, while Group-Fr was driven by clones that have 38 longer remained in Europe. Findings show the ability of such approaches for clonal genotypes 39 identification in grapevines. In particular, we provide evidence on how human actions may have 40 shaped ‘Malbec’ extant genetic diversity pattern.
publishDate 2020
dc.date.none.fl_str_mv 2020-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/138748
Calderón, Pablo Luciano Sebastian; Mauri, Nuria; Muñoz, Claudio Javier; Carbonell Bejerano, Pablo; Bree, Laura; et al.; Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines; Cold Spring Harbor Laboratory Press; bioRxiv; 10-2020; 1-20
2692-8205
CONICET Digital
CONICET
url http://hdl.handle.net/11336/138748
identifier_str_mv Calderón, Pablo Luciano Sebastian; Mauri, Nuria; Muñoz, Claudio Javier; Carbonell Bejerano, Pablo; Bree, Laura; et al.; Clonal propagation history shapes the intra-cultivar genetic diversity in Malbec grapevines; Cold Spring Harbor Laboratory Press; bioRxiv; 10-2020; 1-20
2692-8205
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1101/2020.10.27.356790
info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2020.10.27.356790v1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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