Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamas...

Autores: Yasmin, Mohamad; Rojas, Laura J.; Marshall, Steven H.; Hujer, Andrea M.; Cmolik, Anna; Marshall, Emma; Boucher, Helen W..; Vila, Alejandro Jose; Soldevila, Maxime; Diene, Seydina M.; Rolain, Jean Marc; Bonomo, Robert A.
Año de publicación: 2023
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Background: Elizabethkingia anophelis is an emerging Gram-negative nonlactose fermenter in the health care setting, where it causes life-threatening infections in immunocompromised patients. We aimed to characterize the molecular mechanisms of antimicrobial resistance and evaluate the utility of contemporary antibiotics with the intent to offer targeted therapy against an uncommonly encountered pathogen.Methods: Whole-genome sequencing (WGS) was conducted to accurately identify isolate species and elucidate the determinants of β-lactam resistance. Antimicrobial susceptibility testing was performed using broth microdilution and disk diffusion assays. To assess the functional contribution of the major metallo-β-lactamase (MBL) encoding genes to the resistance profile, bla BlaB was cloned into pBCSK(-) phagemid vector and transformed into Escherichia coli DH10B.Results: WGS identified the organism as E. anophelis. MBL genes bla BlaB-1 and bla GOB-26 were identified, in addition to bla CME-2, which encodes for an extended-spectrum β-lactamase (ESBL). Plasmids were not detected. The isolate was nonsusceptible to all commonly available β-lactams, carbapenems, newer β-lactam β-lactamase inhibitor combinations, and to the combination of aztreonam (ATM) with ceftazidime-avibactam (CAZ-AVI). Susceptibility to the novel siderophore cephalosporin cefiderocol was determined. A BlaB-1 transformant E. coli DH10B isolate was obtained and demonstrated increased minimum inhibitory concentrations to cephalosporins, carbapenems, and CAZ-AVI, but not ATM.Conclusions: Using WGS, we accurately identified and characterized an extensively drug-resistant E. anophelis in an immunocompromised patient. Rapid evaluation of the genetic background can guide accurate susceptibility testing to better inform antimicrobial therapy selection.
Fil: Yasmin, Mohamad. Case Western Reserve University; Estados Unidos
Fil: Rojas, Laura J.. Case Western Reserve University; Estados Unidos
Fil: Marshall, Steven H.. Case Western Reserve University; Estados Unidos
Fil: Hujer, Andrea M.. Case Western Reserve University; Estados Unidos
Fil: Cmolik, Anna. Case Western Reserve University; Estados Unidos
Fil: Marshall, Emma. Case Western Reserve University; Estados Unidos
Fil: Boucher, Helen W... No especifíca;
Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Soldevila, Maxime. No especifíca;
Fil: Diene, Seydina M.. Case Western Reserve University; Estados Unidos
Fil: Rolain, Jean Marc. No especifíca;
Fil: Bonomo, Robert A.. Case Western Reserve University; Estados Unidos
Materia: Elizabethkingia
cefiderocol
metallo-β-lactamase
whole-genome sequencing.
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/255847

Acceder

id	CONICETDig_3b3fa4b168d67b1f63b59d507b71916b
oai_identifier_str	oai:ri.conicet.gov.ar:11336/255847
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase InhibitorsYasmin, MohamadRojas, Laura J.Marshall, Steven H.Hujer, Andrea M.Cmolik, AnnaMarshall, EmmaBoucher, Helen W..Vila, Alejandro JoseSoldevila, MaximeDiene, Seydina M.Rolain, Jean MarcBonomo, Robert A.Elizabethkingiacefiderocolmetallo-β-lactamasewhole-genome sequencing.https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: Elizabethkingia anophelis is an emerging Gram-negative nonlactose fermenter in the health care setting, where it causes life-threatening infections in immunocompromised patients. We aimed to characterize the molecular mechanisms of antimicrobial resistance and evaluate the utility of contemporary antibiotics with the intent to offer targeted therapy against an uncommonly encountered pathogen.Methods: Whole-genome sequencing (WGS) was conducted to accurately identify isolate species and elucidate the determinants of β-lactam resistance. Antimicrobial susceptibility testing was performed using broth microdilution and disk diffusion assays. To assess the functional contribution of the major metallo-β-lactamase (MBL) encoding genes to the resistance profile, bla BlaB was cloned into pBCSK(-) phagemid vector and transformed into Escherichia coli DH10B.Results: WGS identified the organism as E. anophelis. MBL genes bla BlaB-1 and bla GOB-26 were identified, in addition to bla CME-2, which encodes for an extended-spectrum β-lactamase (ESBL). Plasmids were not detected. The isolate was nonsusceptible to all commonly available β-lactams, carbapenems, newer β-lactam β-lactamase inhibitor combinations, and to the combination of aztreonam (ATM) with ceftazidime-avibactam (CAZ-AVI). Susceptibility to the novel siderophore cephalosporin cefiderocol was determined. A BlaB-1 transformant E. coli DH10B isolate was obtained and demonstrated increased minimum inhibitory concentrations to cephalosporins, carbapenems, and CAZ-AVI, but not ATM.Conclusions: Using WGS, we accurately identified and characterized an extensively drug-resistant E. anophelis in an immunocompromised patient. Rapid evaluation of the genetic background can guide accurate susceptibility testing to better inform antimicrobial therapy selection.Fil: Yasmin, Mohamad. Case Western Reserve University; Estados UnidosFil: Rojas, Laura J.. Case Western Reserve University; Estados UnidosFil: Marshall, Steven H.. Case Western Reserve University; Estados UnidosFil: Hujer, Andrea M.. Case Western Reserve University; Estados UnidosFil: Cmolik, Anna. Case Western Reserve University; Estados UnidosFil: Marshall, Emma. Case Western Reserve University; Estados UnidosFil: Boucher, Helen W... No especifíca;Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Soldevila, Maxime. No especifíca;Fil: Diene, Seydina M.. Case Western Reserve University; Estados UnidosFil: Rolain, Jean Marc. No especifíca;Fil: Bonomo, Robert A.. Case Western Reserve University; Estados UnidosOxford University Press2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/255847Yasmin, Mohamad; Rojas, Laura J.; Marshall, Steven H.; Hujer, Andrea M.; Cmolik, Anna; et al.; Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors; Oxford University Press; Open Forum Infectious Diseases; 10; 2; 2-2023; 1-82328-8957CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/ofid/article/doi/10.1093/ofid/ofad014/7016775info:eu-repo/semantics/altIdentifier/doi/10.1093/ofid/ofad014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:10Zoai:ri.conicet.gov.ar:11336/255847instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:10.986CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors
title	Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors
spellingShingle	Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors Yasmin, Mohamad Elizabethkingia cefiderocol metallo-β-lactamase whole-genome sequencing.
title_short	Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors
title_full	Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors
title_fullStr	Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors
title_full_unstemmed	Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors
title_sort	Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors
dc.creator.none.fl_str_mv	Yasmin, Mohamad Rojas, Laura J. Marshall, Steven H. Hujer, Andrea M. Cmolik, Anna Marshall, Emma Boucher, Helen W.. Vila, Alejandro Jose Soldevila, Maxime Diene, Seydina M. Rolain, Jean Marc Bonomo, Robert A.
author	Yasmin, Mohamad
author_facet	Yasmin, Mohamad Rojas, Laura J. Marshall, Steven H. Hujer, Andrea M. Cmolik, Anna Marshall, Emma Boucher, Helen W.. Vila, Alejandro Jose Soldevila, Maxime Diene, Seydina M. Rolain, Jean Marc Bonomo, Robert A.
author_role	author
author2	Rojas, Laura J. Marshall, Steven H. Hujer, Andrea M. Cmolik, Anna Marshall, Emma Boucher, Helen W.. Vila, Alejandro Jose Soldevila, Maxime Diene, Seydina M. Rolain, Jean Marc Bonomo, Robert A.
author2_role	author author author author author author author author author author author
dc.subject.none.fl_str_mv	Elizabethkingia cefiderocol metallo-β-lactamase whole-genome sequencing.
topic	Elizabethkingia cefiderocol metallo-β-lactamase whole-genome sequencing.
purl_subject.fl_str_mv	https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv	Background: Elizabethkingia anophelis is an emerging Gram-negative nonlactose fermenter in the health care setting, where it causes life-threatening infections in immunocompromised patients. We aimed to characterize the molecular mechanisms of antimicrobial resistance and evaluate the utility of contemporary antibiotics with the intent to offer targeted therapy against an uncommonly encountered pathogen.Methods: Whole-genome sequencing (WGS) was conducted to accurately identify isolate species and elucidate the determinants of β-lactam resistance. Antimicrobial susceptibility testing was performed using broth microdilution and disk diffusion assays. To assess the functional contribution of the major metallo-β-lactamase (MBL) encoding genes to the resistance profile, bla BlaB was cloned into pBCSK(-) phagemid vector and transformed into Escherichia coli DH10B.Results: WGS identified the organism as E. anophelis. MBL genes bla BlaB-1 and bla GOB-26 were identified, in addition to bla CME-2, which encodes for an extended-spectrum β-lactamase (ESBL). Plasmids were not detected. The isolate was nonsusceptible to all commonly available β-lactams, carbapenems, newer β-lactam β-lactamase inhibitor combinations, and to the combination of aztreonam (ATM) with ceftazidime-avibactam (CAZ-AVI). Susceptibility to the novel siderophore cephalosporin cefiderocol was determined. A BlaB-1 transformant E. coli DH10B isolate was obtained and demonstrated increased minimum inhibitory concentrations to cephalosporins, carbapenems, and CAZ-AVI, but not ATM.Conclusions: Using WGS, we accurately identified and characterized an extensively drug-resistant E. anophelis in an immunocompromised patient. Rapid evaluation of the genetic background can guide accurate susceptibility testing to better inform antimicrobial therapy selection. Fil: Yasmin, Mohamad. Case Western Reserve University; Estados Unidos Fil: Rojas, Laura J.. Case Western Reserve University; Estados Unidos Fil: Marshall, Steven H.. Case Western Reserve University; Estados Unidos Fil: Hujer, Andrea M.. Case Western Reserve University; Estados Unidos Fil: Cmolik, Anna. Case Western Reserve University; Estados Unidos Fil: Marshall, Emma. Case Western Reserve University; Estados Unidos Fil: Boucher, Helen W... No especifíca; Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Soldevila, Maxime. No especifíca; Fil: Diene, Seydina M.. Case Western Reserve University; Estados Unidos Fil: Rolain, Jean Marc. No especifíca; Fil: Bonomo, Robert A.. Case Western Reserve University; Estados Unidos
description	Background: Elizabethkingia anophelis is an emerging Gram-negative nonlactose fermenter in the health care setting, where it causes life-threatening infections in immunocompromised patients. We aimed to characterize the molecular mechanisms of antimicrobial resistance and evaluate the utility of contemporary antibiotics with the intent to offer targeted therapy against an uncommonly encountered pathogen.Methods: Whole-genome sequencing (WGS) was conducted to accurately identify isolate species and elucidate the determinants of β-lactam resistance. Antimicrobial susceptibility testing was performed using broth microdilution and disk diffusion assays. To assess the functional contribution of the major metallo-β-lactamase (MBL) encoding genes to the resistance profile, bla BlaB was cloned into pBCSK(-) phagemid vector and transformed into Escherichia coli DH10B.Results: WGS identified the organism as E. anophelis. MBL genes bla BlaB-1 and bla GOB-26 were identified, in addition to bla CME-2, which encodes for an extended-spectrum β-lactamase (ESBL). Plasmids were not detected. The isolate was nonsusceptible to all commonly available β-lactams, carbapenems, newer β-lactam β-lactamase inhibitor combinations, and to the combination of aztreonam (ATM) with ceftazidime-avibactam (CAZ-AVI). Susceptibility to the novel siderophore cephalosporin cefiderocol was determined. A BlaB-1 transformant E. coli DH10B isolate was obtained and demonstrated increased minimum inhibitory concentrations to cephalosporins, carbapenems, and CAZ-AVI, but not ATM.Conclusions: Using WGS, we accurately identified and characterized an extensively drug-resistant E. anophelis in an immunocompromised patient. Rapid evaluation of the genetic background can guide accurate susceptibility testing to better inform antimicrobial therapy selection.
publishDate	2023
dc.date.none.fl_str_mv	2023-02
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/255847 Yasmin, Mohamad; Rojas, Laura J.; Marshall, Steven H.; Hujer, Andrea M.; Cmolik, Anna; et al.; Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors; Oxford University Press; Open Forum Infectious Diseases; 10; 2; 2-2023; 1-8 2328-8957 CONICET Digital CONICET
url	http://hdl.handle.net/11336/255847
identifier_str_mv	Yasmin, Mohamad; Rojas, Laura J.; Marshall, Steven H.; Hujer, Andrea M.; Cmolik, Anna; et al.; Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors; Oxford University Press; Open Forum Infectious Diseases; 10; 2; 2-2023; 1-8 2328-8957 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/ofid/article/doi/10.1093/ofid/ofad014/7016775 info:eu-repo/semantics/altIdentifier/doi/10.1093/ofid/ofad014
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Oxford University Press
publisher.none.fl_str_mv	Oxford University Press
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1844613757365387264
score	13.070432

Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis —Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamas...

Publicaciones similares