Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor
- Autores
- Covaleda Cortes, Giovanni; Hernández, Martha; Trejo, Sebastian Alejandro; Mansur, Manuel; Rodriguez Calado, Sergi; Garcia Pardo, Javier; Lorenzo, Julia; Vendrell, Josep; Chávez, María Ángeles; Alonso Del Rivero, Maday; Avilés, Francesc Xavier
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A very powerful proteinaceous inhibitor of metallocarboxypeptidases has been isolated from the marine snail Nerita versicolor and characterized in depth. The most abundant of four, very similar isoforms, NvCla, was taken as reference and N-terminally sequenced to obtain a 372-nucleotide band coding for the protein cDNA. The mature protein contains 53 residues and three disulphide bonds. NvCIa and the other isoforms show an exceptionally high inhibitory capacity of around 1.8 pM for human Carboxypeptidase A1 (hCPA1) and for other A-like members of the M14 CPAsubfamily, whereas a twofold decrease in inhibitory potency is observed for carboxypeptidase B-like members as hCPB and hTAFIa. A recombinant form, rNvCI, was produced in high yield and HPLC, mass spectrometry and spectroscopic analyses by CD and NMR indicated its homogeneous, compact and thermally resistant nature. Using antibodies raised with rNvCI and histochemical analyses, a preferential distribution of the inhibitor in the surface regions of the animal body was observed, particularly nearby the open entrance of the shell and gut, suggesting its involvement in biological defense mechanisms. The properties of this strong, small and stable inhibitor of metallocarboxypeptidases envisage potentialities for its direct applicability, as well as leading or minimized forms, in biotechnological/biomedical uses.
Fil: Covaleda Cortes, Giovanni. Universitat Autònoma de Barcelona; España
Fil: Hernández, Martha. Universidad de Concepción; Chile
Fil: Trejo, Sebastian Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universitat Autònoma de Barcelona; España. YPF - Tecnología; Argentina
Fil: Mansur, Manuel. Universitat Autònoma de Barcelona; España
Fil: Rodriguez Calado, Sergi. Universitat Autònoma de Barcelona; España
Fil: Garcia Pardo, Javier. Universitat Autònoma de Barcelona; España
Fil: Lorenzo, Julia. Universitat Autònoma de Barcelona; España
Fil: Vendrell, Josep. Universitat Autònoma de Barcelona; España
Fil: Chávez, María Ángeles. Universidad de la Habana. Facultad de Biología. Centro de Estudios de Las Proteinas; Cuba
Fil: Alonso Del Rivero, Maday. Universidad de la Habana. Facultad de Biología. Centro de Estudios de Las Proteinas; Cuba
Fil: Avilés, Francesc Xavier. Universitat Autònoma de Barcelona; España - Materia
-
BIOMEDICAL APPLICATIONS
BIOTECHNOLOGICAL
CARBOXYPEPTIDASE
NERITA VERSICOLOR
PICOMOLAR INHIBITION
PROTEINACEOUS INHIBITOR
RECOMBINANT PRODUCTION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/159977
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/159977 |
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3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolorCovaleda Cortes, GiovanniHernández, MarthaTrejo, Sebastian AlejandroMansur, ManuelRodriguez Calado, SergiGarcia Pardo, JavierLorenzo, JuliaVendrell, JosepChávez, María ÁngelesAlonso Del Rivero, MadayAvilés, Francesc XavierBIOMEDICAL APPLICATIONSBIOTECHNOLOGICALCARBOXYPEPTIDASENERITA VERSICOLORPICOMOLAR INHIBITIONPROTEINACEOUS INHIBITORRECOMBINANT PRODUCTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A very powerful proteinaceous inhibitor of metallocarboxypeptidases has been isolated from the marine snail Nerita versicolor and characterized in depth. The most abundant of four, very similar isoforms, NvCla, was taken as reference and N-terminally sequenced to obtain a 372-nucleotide band coding for the protein cDNA. The mature protein contains 53 residues and three disulphide bonds. NvCIa and the other isoforms show an exceptionally high inhibitory capacity of around 1.8 pM for human Carboxypeptidase A1 (hCPA1) and for other A-like members of the M14 CPAsubfamily, whereas a twofold decrease in inhibitory potency is observed for carboxypeptidase B-like members as hCPB and hTAFIa. A recombinant form, rNvCI, was produced in high yield and HPLC, mass spectrometry and spectroscopic analyses by CD and NMR indicated its homogeneous, compact and thermally resistant nature. Using antibodies raised with rNvCI and histochemical analyses, a preferential distribution of the inhibitor in the surface regions of the animal body was observed, particularly nearby the open entrance of the shell and gut, suggesting its involvement in biological defense mechanisms. The properties of this strong, small and stable inhibitor of metallocarboxypeptidases envisage potentialities for its direct applicability, as well as leading or minimized forms, in biotechnological/biomedical uses.Fil: Covaleda Cortes, Giovanni. Universitat Autònoma de Barcelona; EspañaFil: Hernández, Martha. Universidad de Concepción; ChileFil: Trejo, Sebastian Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universitat Autònoma de Barcelona; España. YPF - Tecnología; ArgentinaFil: Mansur, Manuel. Universitat Autònoma de Barcelona; EspañaFil: Rodriguez Calado, Sergi. Universitat Autònoma de Barcelona; EspañaFil: Garcia Pardo, Javier. Universitat Autònoma de Barcelona; EspañaFil: Lorenzo, Julia. Universitat Autònoma de Barcelona; EspañaFil: Vendrell, Josep. Universitat Autònoma de Barcelona; EspañaFil: Chávez, María Ángeles. Universidad de la Habana. Facultad de Biología. Centro de Estudios de Las Proteinas; CubaFil: Alonso Del Rivero, Maday. Universidad de la Habana. Facultad de Biología. Centro de Estudios de Las Proteinas; CubaFil: Avilés, Francesc Xavier. Universitat Autònoma de Barcelona; EspañaMultidisciplinary Digital Publishing Institute2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159977Covaleda Cortes, Giovanni; Hernández, Martha; Trejo, Sebastian Alejandro; Mansur, Manuel; Rodriguez Calado, Sergi; et al.; Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor; Multidisciplinary Digital Publishing Institute; Marine Drugs; 17; 9; 8-2019; 1-191660-3397CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1660-3397/17/9/511info:eu-repo/semantics/altIdentifier/doi/10.3390/md17090511info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:56Zoai:ri.conicet.gov.ar:11336/159977instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:57.14CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor |
title |
Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor |
spellingShingle |
Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor Covaleda Cortes, Giovanni BIOMEDICAL APPLICATIONS BIOTECHNOLOGICAL CARBOXYPEPTIDASE NERITA VERSICOLOR PICOMOLAR INHIBITION PROTEINACEOUS INHIBITOR RECOMBINANT PRODUCTION |
title_short |
Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor |
title_full |
Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor |
title_fullStr |
Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor |
title_full_unstemmed |
Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor |
title_sort |
Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor |
dc.creator.none.fl_str_mv |
Covaleda Cortes, Giovanni Hernández, Martha Trejo, Sebastian Alejandro Mansur, Manuel Rodriguez Calado, Sergi Garcia Pardo, Javier Lorenzo, Julia Vendrell, Josep Chávez, María Ángeles Alonso Del Rivero, Maday Avilés, Francesc Xavier |
author |
Covaleda Cortes, Giovanni |
author_facet |
Covaleda Cortes, Giovanni Hernández, Martha Trejo, Sebastian Alejandro Mansur, Manuel Rodriguez Calado, Sergi Garcia Pardo, Javier Lorenzo, Julia Vendrell, Josep Chávez, María Ángeles Alonso Del Rivero, Maday Avilés, Francesc Xavier |
author_role |
author |
author2 |
Hernández, Martha Trejo, Sebastian Alejandro Mansur, Manuel Rodriguez Calado, Sergi Garcia Pardo, Javier Lorenzo, Julia Vendrell, Josep Chávez, María Ángeles Alonso Del Rivero, Maday Avilés, Francesc Xavier |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
BIOMEDICAL APPLICATIONS BIOTECHNOLOGICAL CARBOXYPEPTIDASE NERITA VERSICOLOR PICOMOLAR INHIBITION PROTEINACEOUS INHIBITOR RECOMBINANT PRODUCTION |
topic |
BIOMEDICAL APPLICATIONS BIOTECHNOLOGICAL CARBOXYPEPTIDASE NERITA VERSICOLOR PICOMOLAR INHIBITION PROTEINACEOUS INHIBITOR RECOMBINANT PRODUCTION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A very powerful proteinaceous inhibitor of metallocarboxypeptidases has been isolated from the marine snail Nerita versicolor and characterized in depth. The most abundant of four, very similar isoforms, NvCla, was taken as reference and N-terminally sequenced to obtain a 372-nucleotide band coding for the protein cDNA. The mature protein contains 53 residues and three disulphide bonds. NvCIa and the other isoforms show an exceptionally high inhibitory capacity of around 1.8 pM for human Carboxypeptidase A1 (hCPA1) and for other A-like members of the M14 CPAsubfamily, whereas a twofold decrease in inhibitory potency is observed for carboxypeptidase B-like members as hCPB and hTAFIa. A recombinant form, rNvCI, was produced in high yield and HPLC, mass spectrometry and spectroscopic analyses by CD and NMR indicated its homogeneous, compact and thermally resistant nature. Using antibodies raised with rNvCI and histochemical analyses, a preferential distribution of the inhibitor in the surface regions of the animal body was observed, particularly nearby the open entrance of the shell and gut, suggesting its involvement in biological defense mechanisms. The properties of this strong, small and stable inhibitor of metallocarboxypeptidases envisage potentialities for its direct applicability, as well as leading or minimized forms, in biotechnological/biomedical uses. Fil: Covaleda Cortes, Giovanni. Universitat Autònoma de Barcelona; España Fil: Hernández, Martha. Universidad de Concepción; Chile Fil: Trejo, Sebastian Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universitat Autònoma de Barcelona; España. YPF - Tecnología; Argentina Fil: Mansur, Manuel. Universitat Autònoma de Barcelona; España Fil: Rodriguez Calado, Sergi. Universitat Autònoma de Barcelona; España Fil: Garcia Pardo, Javier. Universitat Autònoma de Barcelona; España Fil: Lorenzo, Julia. Universitat Autònoma de Barcelona; España Fil: Vendrell, Josep. Universitat Autònoma de Barcelona; España Fil: Chávez, María Ángeles. Universidad de la Habana. Facultad de Biología. Centro de Estudios de Las Proteinas; Cuba Fil: Alonso Del Rivero, Maday. Universidad de la Habana. Facultad de Biología. Centro de Estudios de Las Proteinas; Cuba Fil: Avilés, Francesc Xavier. Universitat Autònoma de Barcelona; España |
description |
A very powerful proteinaceous inhibitor of metallocarboxypeptidases has been isolated from the marine snail Nerita versicolor and characterized in depth. The most abundant of four, very similar isoforms, NvCla, was taken as reference and N-terminally sequenced to obtain a 372-nucleotide band coding for the protein cDNA. The mature protein contains 53 residues and three disulphide bonds. NvCIa and the other isoforms show an exceptionally high inhibitory capacity of around 1.8 pM for human Carboxypeptidase A1 (hCPA1) and for other A-like members of the M14 CPAsubfamily, whereas a twofold decrease in inhibitory potency is observed for carboxypeptidase B-like members as hCPB and hTAFIa. A recombinant form, rNvCI, was produced in high yield and HPLC, mass spectrometry and spectroscopic analyses by CD and NMR indicated its homogeneous, compact and thermally resistant nature. Using antibodies raised with rNvCI and histochemical analyses, a preferential distribution of the inhibitor in the surface regions of the animal body was observed, particularly nearby the open entrance of the shell and gut, suggesting its involvement in biological defense mechanisms. The properties of this strong, small and stable inhibitor of metallocarboxypeptidases envisage potentialities for its direct applicability, as well as leading or minimized forms, in biotechnological/biomedical uses. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/159977 Covaleda Cortes, Giovanni; Hernández, Martha; Trejo, Sebastian Alejandro; Mansur, Manuel; Rodriguez Calado, Sergi; et al.; Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor; Multidisciplinary Digital Publishing Institute; Marine Drugs; 17; 9; 8-2019; 1-19 1660-3397 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/159977 |
identifier_str_mv |
Covaleda Cortes, Giovanni; Hernández, Martha; Trejo, Sebastian Alejandro; Mansur, Manuel; Rodriguez Calado, Sergi; et al.; Characterization, recombinant production and structure-function analysis of NvCI, a picomolar metallocarboxypeptidase inhibitor from the marine nnail Nerita versicolor; Multidisciplinary Digital Publishing Institute; Marine Drugs; 17; 9; 8-2019; 1-19 1660-3397 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1660-3397/17/9/511 info:eu-repo/semantics/altIdentifier/doi/10.3390/md17090511 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613463864770560 |
score |
13.070432 |