HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells

Autores
Banda, María M.; López, María Carolina; Manzo, Rubiceli; Rico Pérez, Gadea; García, Pablo; Rosales Reyes, Roberto; De La Cruz, Miguel A.; Soncini, Fernando Carlos; García del Portillo, Francisco; Bustamante, Víctor H.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
When Salmonella is grown in the nutrient-rich lysogeny broth (LB), the AraC-like transcriptional regulator HilD positively controls the expression of genes required for Salmonella invasion of host cells, such as the Salmonella pathogenicity island 1 (SPI-1) genes. However, in minimal media, the two-component system PhoP/Q activates the expression of genes necessary for Salmonella replication inside host cells, such as the SPI-2 genes. Recently, we found that the SL1344-1872 hypothetical gene, located in a S. Typhimurium genomic island, is co-expressed with the SPI-1 genes. In this study we demonstrate that HilD induces indirectly the expression of SL1344-1872 when S. Typhimurium is grown in LB; therefore, we named SL1344-1872 as grhD1 for gene regulated by HilD. Furthermore, we found that PhoP positively controls the expression of grhD1, independently of HilD, when S. Typhimurium is grown in LB or N-minimal medium. Moreover, we demonstrate that the grhD1 gene is required for the invasion of S. Typhimurium into epithelial cells, macrophages and fibroblasts, as well as for the intestinal inflammatory response caused by S. Typhimurium in mice. Thus, our results reveal a novel virulence factor of Salmonella, whose expression is positively and independently controlled by the HilD and PhoP transcriptional regulators.
Fil: Banda, María M.. Universidad Nacional Autónoma de México; México
Fil: López, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Manzo, Rubiceli. Universidad Nacional Autónoma de México; México
Fil: Rico Pérez, Gadea. Consejo Superior de Investigaciones Científicas; España
Fil: García, Pablo. Consejo Superior de Investigaciones Científicas; España
Fil: Rosales Reyes, Roberto. Universidad Nacional Autónoma de México; México
Fil: De La Cruz, Miguel A.. Centro Médico Nacional Siglo XXI; México
Fil: Soncini, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: García del Portillo, Francisco. Consejo Superior de Investigaciones Científicas; España
Fil: Bustamante, Víctor H.. Universidad Nacional Autónoma de México; México
Materia
SALMONELLA ENTERICA
TRANSCRIPTIONAL REGULATION
VIRULENCE FACTOR
PATHOGENESIS
HILD/PHOP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/89740
Acceder
id CONICETDig_3a52a9b17cf77541c988aa05d94a1304
oai_identifier_str oai:ri.conicet.gov.ar:11336/89740
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cellsBanda, María M.López, María CarolinaManzo, RubiceliRico Pérez, GadeaGarcía, PabloRosales Reyes, RobertoDe La Cruz, Miguel A.Soncini, Fernando CarlosGarcía del Portillo, FranciscoBustamante, Víctor H.SALMONELLA ENTERICATRANSCRIPTIONAL REGULATIONVIRULENCE FACTORPATHOGENESISHILD/PHOPhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1When Salmonella is grown in the nutrient-rich lysogeny broth (LB), the AraC-like transcriptional regulator HilD positively controls the expression of genes required for Salmonella invasion of host cells, such as the Salmonella pathogenicity island 1 (SPI-1) genes. However, in minimal media, the two-component system PhoP/Q activates the expression of genes necessary for Salmonella replication inside host cells, such as the SPI-2 genes. Recently, we found that the SL1344-1872 hypothetical gene, located in a S. Typhimurium genomic island, is co-expressed with the SPI-1 genes. In this study we demonstrate that HilD induces indirectly the expression of SL1344-1872 when S. Typhimurium is grown in LB; therefore, we named SL1344-1872 as grhD1 for gene regulated by HilD. Furthermore, we found that PhoP positively controls the expression of grhD1, independently of HilD, when S. Typhimurium is grown in LB or N-minimal medium. Moreover, we demonstrate that the grhD1 gene is required for the invasion of S. Typhimurium into epithelial cells, macrophages and fibroblasts, as well as for the intestinal inflammatory response caused by S. Typhimurium in mice. Thus, our results reveal a novel virulence factor of Salmonella, whose expression is positively and independently controlled by the HilD and PhoP transcriptional regulators.Fil: Banda, María M.. Universidad Nacional Autónoma de México; MéxicoFil: López, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Manzo, Rubiceli. Universidad Nacional Autónoma de México; MéxicoFil: Rico Pérez, Gadea. Consejo Superior de Investigaciones Científicas; EspañaFil: García, Pablo. Consejo Superior de Investigaciones Científicas; EspañaFil: Rosales Reyes, Roberto. Universidad Nacional Autónoma de México; MéxicoFil: De La Cruz, Miguel A.. Centro Médico Nacional Siglo XXI; MéxicoFil: Soncini, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: García del Portillo, Francisco. Consejo Superior de Investigaciones Científicas; EspañaFil: Bustamante, Víctor H.. Universidad Nacional Autónoma de México; MéxicoNature Publishing Group2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89740Banda, María M.; López, María Carolina; Manzo, Rubiceli; Rico Pérez, Gadea; García, Pablo; et al.; HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-152045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-018-23068-0info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-23068-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:32Zoai:ri.conicet.gov.ar:11336/89740instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:32.367CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells
title HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells
spellingShingle HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells
Banda, María M.
SALMONELLA ENTERICA
TRANSCRIPTIONAL REGULATION
VIRULENCE FACTOR
PATHOGENESIS
HILD/PHOP
title_short HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells
title_full HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells
title_fullStr HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells
title_full_unstemmed HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells
title_sort HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells
dc.creator.none.fl_str_mv Banda, María M.
López, María Carolina
Manzo, Rubiceli
Rico Pérez, Gadea
García, Pablo
Rosales Reyes, Roberto
De La Cruz, Miguel A.
Soncini, Fernando Carlos
García del Portillo, Francisco
Bustamante, Víctor H.
author Banda, María M.
author_facet Banda, María M.
López, María Carolina
Manzo, Rubiceli
Rico Pérez, Gadea
García, Pablo
Rosales Reyes, Roberto
De La Cruz, Miguel A.
Soncini, Fernando Carlos
García del Portillo, Francisco
Bustamante, Víctor H.
author_role author
author2 López, María Carolina
Manzo, Rubiceli
Rico Pérez, Gadea
García, Pablo
Rosales Reyes, Roberto
De La Cruz, Miguel A.
Soncini, Fernando Carlos
García del Portillo, Francisco
Bustamante, Víctor H.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SALMONELLA ENTERICA
TRANSCRIPTIONAL REGULATION
VIRULENCE FACTOR
PATHOGENESIS
HILD/PHOP
topic SALMONELLA ENTERICA
TRANSCRIPTIONAL REGULATION
VIRULENCE FACTOR
PATHOGENESIS
HILD/PHOP
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv When Salmonella is grown in the nutrient-rich lysogeny broth (LB), the AraC-like transcriptional regulator HilD positively controls the expression of genes required for Salmonella invasion of host cells, such as the Salmonella pathogenicity island 1 (SPI-1) genes. However, in minimal media, the two-component system PhoP/Q activates the expression of genes necessary for Salmonella replication inside host cells, such as the SPI-2 genes. Recently, we found that the SL1344-1872 hypothetical gene, located in a S. Typhimurium genomic island, is co-expressed with the SPI-1 genes. In this study we demonstrate that HilD induces indirectly the expression of SL1344-1872 when S. Typhimurium is grown in LB; therefore, we named SL1344-1872 as grhD1 for gene regulated by HilD. Furthermore, we found that PhoP positively controls the expression of grhD1, independently of HilD, when S. Typhimurium is grown in LB or N-minimal medium. Moreover, we demonstrate that the grhD1 gene is required for the invasion of S. Typhimurium into epithelial cells, macrophages and fibroblasts, as well as for the intestinal inflammatory response caused by S. Typhimurium in mice. Thus, our results reveal a novel virulence factor of Salmonella, whose expression is positively and independently controlled by the HilD and PhoP transcriptional regulators.
Fil: Banda, María M.. Universidad Nacional Autónoma de México; México
Fil: López, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Manzo, Rubiceli. Universidad Nacional Autónoma de México; México
Fil: Rico Pérez, Gadea. Consejo Superior de Investigaciones Científicas; España
Fil: García, Pablo. Consejo Superior de Investigaciones Científicas; España
Fil: Rosales Reyes, Roberto. Universidad Nacional Autónoma de México; México
Fil: De La Cruz, Miguel A.. Centro Médico Nacional Siglo XXI; México
Fil: Soncini, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: García del Portillo, Francisco. Consejo Superior de Investigaciones Científicas; España
Fil: Bustamante, Víctor H.. Universidad Nacional Autónoma de México; México
description When Salmonella is grown in the nutrient-rich lysogeny broth (LB), the AraC-like transcriptional regulator HilD positively controls the expression of genes required for Salmonella invasion of host cells, such as the Salmonella pathogenicity island 1 (SPI-1) genes. However, in minimal media, the two-component system PhoP/Q activates the expression of genes necessary for Salmonella replication inside host cells, such as the SPI-2 genes. Recently, we found that the SL1344-1872 hypothetical gene, located in a S. Typhimurium genomic island, is co-expressed with the SPI-1 genes. In this study we demonstrate that HilD induces indirectly the expression of SL1344-1872 when S. Typhimurium is grown in LB; therefore, we named SL1344-1872 as grhD1 for gene regulated by HilD. Furthermore, we found that PhoP positively controls the expression of grhD1, independently of HilD, when S. Typhimurium is grown in LB or N-minimal medium. Moreover, we demonstrate that the grhD1 gene is required for the invasion of S. Typhimurium into epithelial cells, macrophages and fibroblasts, as well as for the intestinal inflammatory response caused by S. Typhimurium in mice. Thus, our results reveal a novel virulence factor of Salmonella, whose expression is positively and independently controlled by the HilD and PhoP transcriptional regulators.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/89740
Banda, María M.; López, María Carolina; Manzo, Rubiceli; Rico Pérez, Gadea; García, Pablo; et al.; HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-15
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/89740
identifier_str_mv Banda, María M.; López, María Carolina; Manzo, Rubiceli; Rico Pérez, Gadea; García, Pablo; et al.; HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-15
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-018-23068-0
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-23068-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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