A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202

Autores
Páez, María Augusta; Casa Villegas, Mary; Naranjo Moreno, Vanesa; Félix, Neyda Espín; Cabezas Terán, Katty; Andreatta, Alfonsina Ester
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The primary application of membrane technology is the separation and purification of biotechnological products, with proteins and enzymes being integral components of these processes. Fouling of membranes during crossflow filtration is a prominent challenge. This work aims to evaluate filtration performance and fouling, particularly related to pore-blocking mechanisms, throughout one crossflow microfiltration (MF) and six consecutive ultrafiltration (UF) stages implemented for laccase extracts produced by Pleurotus ostreatus 202 fungi. Activity concentration factor, recovery efficiency and purification factor are the key performance criteria. To assess membrane fouling, dead-end filtration models formulated in 1982 were compared to crossflow filtration models developed in 2011. The experimental flux decline in all filtration stages is satisfactorily described by crossflow models accounting for removal term during pore-blocking, so R2 is an appropriate fitting parameter.Complete pore blocking mechanism significantly impacts the MF, UF 1000, UF 100 and UF 10 stages, with the highest filtration constant (KbF) estimated at 12.60×10-4 (s-1). Conventional standard pore blocking is evident in all filtration stages, but the lowest impact on fouling is observed since the progressive flux decline. Although intermediate pore blocking mechanism appears in all filtration stages, UF 100 is the most affected, with a filtration constant (KiF) of 16.70 (m-1). This tendency is confirmed by the highest purification factor (6.95) and the 65, 62 and 56 kDa laccasses found in the retentate. Finally, the absence of cake formation and the permeate volume recovered indicates that neither membrane was exposed to exhaustive fouling that could not be reversed by backwashing.
Fil: Páez, María Augusta. Escuela Politécnica Nacional; Ecuador
Fil: Casa Villegas, Mary. Escuela Politécnica Nacional; Ecuador
Fil: Naranjo Moreno, Vanesa. Escuela Politécnica Nacional; Ecuador
Fil: Félix, Neyda Espín. Escuela Politécnica Nacional; Ecuador
Fil: Cabezas Terán, Katty. Escuela Politécnica Nacional; Ecuador
Fil: Andreatta, Alfonsina Ester. Universidad Tecnológica Nacional. Facultad Reg.san Francisco. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
FOULING
CROSSFLOW FILTRATION
PORE BLOCKING
PERMEATE FLUX
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272779
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272779
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202Páez, María AugustaCasa Villegas, MaryNaranjo Moreno, VanesaFélix, Neyda EspínCabezas Terán, KattyAndreatta, Alfonsina EsterFOULINGCROSSFLOW FILTRATIONPORE BLOCKINGPERMEATE FLUXhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2The primary application of membrane technology is the separation and purification of biotechnological products, with proteins and enzymes being integral components of these processes. Fouling of membranes during crossflow filtration is a prominent challenge. This work aims to evaluate filtration performance and fouling, particularly related to pore-blocking mechanisms, throughout one crossflow microfiltration (MF) and six consecutive ultrafiltration (UF) stages implemented for laccase extracts produced by Pleurotus ostreatus 202 fungi. Activity concentration factor, recovery efficiency and purification factor are the key performance criteria. To assess membrane fouling, dead-end filtration models formulated in 1982 were compared to crossflow filtration models developed in 2011. The experimental flux decline in all filtration stages is satisfactorily described by crossflow models accounting for removal term during pore-blocking, so R2 is an appropriate fitting parameter.Complete pore blocking mechanism significantly impacts the MF, UF 1000, UF 100 and UF 10 stages, with the highest filtration constant (KbF) estimated at 12.60×10-4 (s-1). Conventional standard pore blocking is evident in all filtration stages, but the lowest impact on fouling is observed since the progressive flux decline. Although intermediate pore blocking mechanism appears in all filtration stages, UF 100 is the most affected, with a filtration constant (KiF) of 16.70 (m-1). This tendency is confirmed by the highest purification factor (6.95) and the 65, 62 and 56 kDa laccasses found in the retentate. Finally, the absence of cake formation and the permeate volume recovered indicates that neither membrane was exposed to exhaustive fouling that could not be reversed by backwashing.Fil: Páez, María Augusta. Escuela Politécnica Nacional; EcuadorFil: Casa Villegas, Mary. Escuela Politécnica Nacional; EcuadorFil: Naranjo Moreno, Vanesa. Escuela Politécnica Nacional; EcuadorFil: Félix, Neyda Espín. Escuela Politécnica Nacional; EcuadorFil: Cabezas Terán, Katty. Escuela Politécnica Nacional; EcuadorFil: Andreatta, Alfonsina Ester. Universidad Tecnológica Nacional. Facultad Reg.san Francisco. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaMDPI2025-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272779Páez, María Augusta; Casa Villegas, Mary; Naranjo Moreno, Vanesa; Félix, Neyda Espín; Cabezas Terán, Katty; et al.; A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202; MDPI; Membranes; 15; 8; 7-2025; 1-172077-0375CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2077-0375/15/8/226info:eu-repo/semantics/altIdentifier/doi/10.3390/membranes15080226info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:56:09Zoai:ri.conicet.gov.ar:11336/272779instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:56:10.12CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202
title A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202
spellingShingle A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202
Páez, María Augusta
FOULING
CROSSFLOW FILTRATION
PORE BLOCKING
PERMEATE FLUX
title_short A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202
title_full A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202
title_fullStr A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202
title_full_unstemmed A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202
title_sort A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202
dc.creator.none.fl_str_mv Páez, María Augusta
Casa Villegas, Mary
Naranjo Moreno, Vanesa
Félix, Neyda Espín
Cabezas Terán, Katty
Andreatta, Alfonsina Ester
author Páez, María Augusta
author_facet Páez, María Augusta
Casa Villegas, Mary
Naranjo Moreno, Vanesa
Félix, Neyda Espín
Cabezas Terán, Katty
Andreatta, Alfonsina Ester
author_role author
author2 Casa Villegas, Mary
Naranjo Moreno, Vanesa
Félix, Neyda Espín
Cabezas Terán, Katty
Andreatta, Alfonsina Ester
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv FOULING
CROSSFLOW FILTRATION
PORE BLOCKING
PERMEATE FLUX
topic FOULING
CROSSFLOW FILTRATION
PORE BLOCKING
PERMEATE FLUX
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The primary application of membrane technology is the separation and purification of biotechnological products, with proteins and enzymes being integral components of these processes. Fouling of membranes during crossflow filtration is a prominent challenge. This work aims to evaluate filtration performance and fouling, particularly related to pore-blocking mechanisms, throughout one crossflow microfiltration (MF) and six consecutive ultrafiltration (UF) stages implemented for laccase extracts produced by Pleurotus ostreatus 202 fungi. Activity concentration factor, recovery efficiency and purification factor are the key performance criteria. To assess membrane fouling, dead-end filtration models formulated in 1982 were compared to crossflow filtration models developed in 2011. The experimental flux decline in all filtration stages is satisfactorily described by crossflow models accounting for removal term during pore-blocking, so R2 is an appropriate fitting parameter.Complete pore blocking mechanism significantly impacts the MF, UF 1000, UF 100 and UF 10 stages, with the highest filtration constant (KbF) estimated at 12.60×10-4 (s-1). Conventional standard pore blocking is evident in all filtration stages, but the lowest impact on fouling is observed since the progressive flux decline. Although intermediate pore blocking mechanism appears in all filtration stages, UF 100 is the most affected, with a filtration constant (KiF) of 16.70 (m-1). This tendency is confirmed by the highest purification factor (6.95) and the 65, 62 and 56 kDa laccasses found in the retentate. Finally, the absence of cake formation and the permeate volume recovered indicates that neither membrane was exposed to exhaustive fouling that could not be reversed by backwashing.
Fil: Páez, María Augusta. Escuela Politécnica Nacional; Ecuador
Fil: Casa Villegas, Mary. Escuela Politécnica Nacional; Ecuador
Fil: Naranjo Moreno, Vanesa. Escuela Politécnica Nacional; Ecuador
Fil: Félix, Neyda Espín. Escuela Politécnica Nacional; Ecuador
Fil: Cabezas Terán, Katty. Escuela Politécnica Nacional; Ecuador
Fil: Andreatta, Alfonsina Ester. Universidad Tecnológica Nacional. Facultad Reg.san Francisco. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description The primary application of membrane technology is the separation and purification of biotechnological products, with proteins and enzymes being integral components of these processes. Fouling of membranes during crossflow filtration is a prominent challenge. This work aims to evaluate filtration performance and fouling, particularly related to pore-blocking mechanisms, throughout one crossflow microfiltration (MF) and six consecutive ultrafiltration (UF) stages implemented for laccase extracts produced by Pleurotus ostreatus 202 fungi. Activity concentration factor, recovery efficiency and purification factor are the key performance criteria. To assess membrane fouling, dead-end filtration models formulated in 1982 were compared to crossflow filtration models developed in 2011. The experimental flux decline in all filtration stages is satisfactorily described by crossflow models accounting for removal term during pore-blocking, so R2 is an appropriate fitting parameter.Complete pore blocking mechanism significantly impacts the MF, UF 1000, UF 100 and UF 10 stages, with the highest filtration constant (KbF) estimated at 12.60×10-4 (s-1). Conventional standard pore blocking is evident in all filtration stages, but the lowest impact on fouling is observed since the progressive flux decline. Although intermediate pore blocking mechanism appears in all filtration stages, UF 100 is the most affected, with a filtration constant (KiF) of 16.70 (m-1). This tendency is confirmed by the highest purification factor (6.95) and the 65, 62 and 56 kDa laccasses found in the retentate. Finally, the absence of cake formation and the permeate volume recovered indicates that neither membrane was exposed to exhaustive fouling that could not be reversed by backwashing.
publishDate 2025
dc.date.none.fl_str_mv 2025-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272779
Páez, María Augusta; Casa Villegas, Mary; Naranjo Moreno, Vanesa; Félix, Neyda Espín; Cabezas Terán, Katty; et al.; A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202; MDPI; Membranes; 15; 8; 7-2025; 1-17
2077-0375
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272779
identifier_str_mv Páez, María Augusta; Casa Villegas, Mary; Naranjo Moreno, Vanesa; Félix, Neyda Espín; Cabezas Terán, Katty; et al.; A Model-Driven Approach to Assessing the Fouling Mechanism in the Crossflow Filtration of Laccase Extract from Pleurotus ostreatus 202; MDPI; Membranes; 15; 8; 7-2025; 1-17
2077-0375
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2077-0375/15/8/226
info:eu-repo/semantics/altIdentifier/doi/10.3390/membranes15080226
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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