Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans

Autores
Hernando, Guillermina Silvana; Turani, Ornella; Bouzat, Cecilia Beatriz
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Parasitic nematodes of humans and animals cause dis- eases of major socio-economic importance globally. Con- trol of infections in both human and veterinary medicine currently relies mainly on chemotherapy, but resistance is an increasing problem, so there is an urgent need for discovery of novel drugs. As parasitic nematodes are not ideal laboratory animals, the free-living nematode C. ele- gans was demonstrated to be an excellent model system for the discovery of new anthelmintics and for character- izing their mechanisms of action and resistance. Essen- tial oils (EOs) are natural products produced by aromatic plants. EOs are complex mixtures that contain 2 or 3 ma- jor phytochemicals, which can be terpenes or aromatic compounds. We used paralysis assays of wild-type and mutant C. elegans strain to identify EOs with potential anthelmintic activities, reveal the active components, the target sites and the mechanisms of action. We found that EOs belonging to six different orders produced rap- id paralysis of C. elegans and we established the half maximal effective concentration values between 0.02-1.2 percent of EOs. All EOs tested also inhibited egg hatch- ing, a property related to anthelmintic ability. Thus, EOs mediate both rapid and long-term anthelmintic effects. We determined that trans-cinnamaldehyde (TC), a ma- jor component of C. verum EO, produces both paralysis and egg-hatching inhibition. By testing mutant worms, we identified the muscle L-AChR and GABA receptors as EOs and TC targets in vivo. Thus, by modulating two receptors with key roles in worm motility, these EOs emerge as novel sources of anthelmintic compounds. Likewise, the N-AChR mutant strain is slightly resistant to TC, thus revealing a third target receptor for terpenes. Due to the potential of EOs as sources of novel antipara- sitic compounds, additional studies will be carried out to determine in more detail the molecular mechanisms of action and structure-activity relationships of their active compounds.
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Turani, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínic; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Buenos AIres
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
DRUG THERAPY
anthelmintic compounds
NEMATODE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184774
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis ElegansHernando, Guillermina SilvanaTurani, OrnellaBouzat, Cecilia BeatrizDRUG THERAPYanthelmintic compoundsNEMATODEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Parasitic nematodes of humans and animals cause dis- eases of major socio-economic importance globally. Con- trol of infections in both human and veterinary medicine currently relies mainly on chemotherapy, but resistance is an increasing problem, so there is an urgent need for discovery of novel drugs. As parasitic nematodes are not ideal laboratory animals, the free-living nematode C. ele- gans was demonstrated to be an excellent model system for the discovery of new anthelmintics and for character- izing their mechanisms of action and resistance. Essen- tial oils (EOs) are natural products produced by aromatic plants. EOs are complex mixtures that contain 2 or 3 ma- jor phytochemicals, which can be terpenes or aromatic compounds. We used paralysis assays of wild-type and mutant C. elegans strain to identify EOs with potential anthelmintic activities, reveal the active components, the target sites and the mechanisms of action. We found that EOs belonging to six different orders produced rap- id paralysis of C. elegans and we established the half maximal effective concentration values between 0.02-1.2 percent of EOs. All EOs tested also inhibited egg hatch- ing, a property related to anthelmintic ability. Thus, EOs mediate both rapid and long-term anthelmintic effects. We determined that trans-cinnamaldehyde (TC), a ma- jor component of C. verum EO, produces both paralysis and egg-hatching inhibition. By testing mutant worms, we identified the muscle L-AChR and GABA receptors as EOs and TC targets in vivo. Thus, by modulating two receptors with key roles in worm motility, these EOs emerge as novel sources of anthelmintic compounds. Likewise, the N-AChR mutant strain is slightly resistant to TC, thus revealing a third target receptor for terpenes. Due to the potential of EOs as sources of novel antipara- sitic compounds, additional studies will be carried out to determine in more detail the molecular mechanisms of action and structure-activity relationships of their active compounds.Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Turani, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLXV Reunión Anual de la Sociedad Argentina de Investigación Clínic; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de FisiologíaBuenos AIresArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184774Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínic; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Buenos AIres; Argentina; 2020; 36-360025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:20Zoai:ri.conicet.gov.ar:11336/184774instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:21.242CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans
title Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans
spellingShingle Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans
Hernando, Guillermina Silvana
DRUG THERAPY
anthelmintic compounds
NEMATODE
title_short Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans
title_full Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans
title_fullStr Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans
title_full_unstemmed Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans
title_sort Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans
dc.creator.none.fl_str_mv Hernando, Guillermina Silvana
Turani, Ornella
Bouzat, Cecilia Beatriz
author Hernando, Guillermina Silvana
author_facet Hernando, Guillermina Silvana
Turani, Ornella
Bouzat, Cecilia Beatriz
author_role author
author2 Turani, Ornella
Bouzat, Cecilia Beatriz
author2_role author
author
dc.subject.none.fl_str_mv DRUG THERAPY
anthelmintic compounds
NEMATODE
topic DRUG THERAPY
anthelmintic compounds
NEMATODE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Parasitic nematodes of humans and animals cause dis- eases of major socio-economic importance globally. Con- trol of infections in both human and veterinary medicine currently relies mainly on chemotherapy, but resistance is an increasing problem, so there is an urgent need for discovery of novel drugs. As parasitic nematodes are not ideal laboratory animals, the free-living nematode C. ele- gans was demonstrated to be an excellent model system for the discovery of new anthelmintics and for character- izing their mechanisms of action and resistance. Essen- tial oils (EOs) are natural products produced by aromatic plants. EOs are complex mixtures that contain 2 or 3 ma- jor phytochemicals, which can be terpenes or aromatic compounds. We used paralysis assays of wild-type and mutant C. elegans strain to identify EOs with potential anthelmintic activities, reveal the active components, the target sites and the mechanisms of action. We found that EOs belonging to six different orders produced rap- id paralysis of C. elegans and we established the half maximal effective concentration values between 0.02-1.2 percent of EOs. All EOs tested also inhibited egg hatch- ing, a property related to anthelmintic ability. Thus, EOs mediate both rapid and long-term anthelmintic effects. We determined that trans-cinnamaldehyde (TC), a ma- jor component of C. verum EO, produces both paralysis and egg-hatching inhibition. By testing mutant worms, we identified the muscle L-AChR and GABA receptors as EOs and TC targets in vivo. Thus, by modulating two receptors with key roles in worm motility, these EOs emerge as novel sources of anthelmintic compounds. Likewise, the N-AChR mutant strain is slightly resistant to TC, thus revealing a third target receptor for terpenes. Due to the potential of EOs as sources of novel antipara- sitic compounds, additional studies will be carried out to determine in more detail the molecular mechanisms of action and structure-activity relationships of their active compounds.
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Turani, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínic; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Buenos AIres
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Parasitic nematodes of humans and animals cause dis- eases of major socio-economic importance globally. Con- trol of infections in both human and veterinary medicine currently relies mainly on chemotherapy, but resistance is an increasing problem, so there is an urgent need for discovery of novel drugs. As parasitic nematodes are not ideal laboratory animals, the free-living nematode C. ele- gans was demonstrated to be an excellent model system for the discovery of new anthelmintics and for character- izing their mechanisms of action and resistance. Essen- tial oils (EOs) are natural products produced by aromatic plants. EOs are complex mixtures that contain 2 or 3 ma- jor phytochemicals, which can be terpenes or aromatic compounds. We used paralysis assays of wild-type and mutant C. elegans strain to identify EOs with potential anthelmintic activities, reveal the active components, the target sites and the mechanisms of action. We found that EOs belonging to six different orders produced rap- id paralysis of C. elegans and we established the half maximal effective concentration values between 0.02-1.2 percent of EOs. All EOs tested also inhibited egg hatch- ing, a property related to anthelmintic ability. Thus, EOs mediate both rapid and long-term anthelmintic effects. We determined that trans-cinnamaldehyde (TC), a ma- jor component of C. verum EO, produces both paralysis and egg-hatching inhibition. By testing mutant worms, we identified the muscle L-AChR and GABA receptors as EOs and TC targets in vivo. Thus, by modulating two receptors with key roles in worm motility, these EOs emerge as novel sources of anthelmintic compounds. Likewise, the N-AChR mutant strain is slightly resistant to TC, thus revealing a third target receptor for terpenes. Due to the potential of EOs as sources of novel antipara- sitic compounds, additional studies will be carried out to determine in more detail the molecular mechanisms of action and structure-activity relationships of their active compounds.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184774
Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínic; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Buenos AIres; Argentina; 2020; 36-36
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184774
identifier_str_mv Essential oils for the discovery of new anthelmintic compounds tested on the nematode Caenorhabditis Elegans; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínic; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Buenos AIres; Argentina; 2020; 36-36
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicina
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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