Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum

Autores
Udovin, Lucas; Kobiec, Tamara; Herrera, María Inés; Ramos Hryb, Ana Belen; Toro, Nicolas; Kusnier, Carlos Federico; Capani, Francisco
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Perinatal asphyxia (PA) caused by low O2 availability during birth is associated with brain damage, been the striatum one of the most affected areas. Palmitoylethanolamide (PEA) is a neuroprotective amide in brain injury models, including PA. However, its effects against PA requires deeper study in the striatum. Using Bjelke? model, full-term pregnant rats were rapidly decapitated, and uterus horns placed in a water bath (37°C,19 min). One hour after, rats were treated with PEA (10 mg/kg,s.c.) and later given to surrogate mothers. 30d animals were perfused and coronal striatum sections as well as subcellular fractions were collected for immunohistochemistry and blotting (n=3). Phosphorylated high/medium molecular weight neurofilaments (pNF-H/M), Microtubule-Associated Protein 2 (MAP2), and Glial fibrillary acidic protein (GFAP) were analysed. All procedures were previously approved by our Institution. 2-way ANOVA followed by Tukey revealed a reduction in pNF-H/M and MAP2 reactive areas (45% and 70%), GFAP+ cells (20%) and reduced MAP2 and pNF-H/M immunocontents in PA-rat striatum. PEA totally restored GFAP+ cells and MAP2 immunoreaction and partially prevented the decreased pNF-H/M reactive area (78%) induced by PA. PEA also reversed the MAP2 reduction and partially prevented the decrease of pNF-H/M immunocontent induced by PA. No GFAP level alteration was detected. PEA treatment attenuated the striatum damage induced by PA, demonstrating its therapeutic potential against PA.
Fil: Udovin, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; Argentina
Fil: Kobiec, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Pontificia Universidad Católica de Argentina Santa María de los Buenos Aires. Facutlad de Piscología y Psicopedagogía. Centro de Investigaciones en Psicología y Pedagogía; Argentina
Fil: Herrera, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Pontificia Universidad Católica de Argentina Santa María de los Buenos Aires. Facutlad de Piscología y Psicopedagogía. Centro de Investigaciones en Psicología y Pedagogía; Argentina
Fil: Ramos Hryb, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Toro, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; Argentina
Fil: Kusnier, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; Argentina
Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; Argentina
XXXIII Congreso de la Sociedad Argentina de Investigación en Neurociencias
Córdoba
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
NEUROPROTECTION
STRIATUM
PALMITOYLEHANOLAMIDE palmitoylethanolamide
PERINATAL ASPHYXIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/200769
Acceder
id CONICETDig_38ca1f3a592dcd5cc4401f271333430c
oai_identifier_str oai:ri.conicet.gov.ar:11336/200769
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatumUdovin, LucasKobiec, TamaraHerrera, María InésRamos Hryb, Ana BelenToro, NicolasKusnier, Carlos FedericoCapani, FranciscoNEUROPROTECTIONSTRIATUMPALMITOYLEHANOLAMIDE palmitoylethanolamidePERINATAL ASPHYXIAhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Perinatal asphyxia (PA) caused by low O2 availability during birth is associated with brain damage, been the striatum one of the most affected areas. Palmitoylethanolamide (PEA) is a neuroprotective amide in brain injury models, including PA. However, its effects against PA requires deeper study in the striatum. Using Bjelke? model, full-term pregnant rats were rapidly decapitated, and uterus horns placed in a water bath (37°C,19 min). One hour after, rats were treated with PEA (10 mg/kg,s.c.) and later given to surrogate mothers. 30d animals were perfused and coronal striatum sections as well as subcellular fractions were collected for immunohistochemistry and blotting (n=3). Phosphorylated high/medium molecular weight neurofilaments (pNF-H/M), Microtubule-Associated Protein 2 (MAP2), and Glial fibrillary acidic protein (GFAP) were analysed. All procedures were previously approved by our Institution. 2-way ANOVA followed by Tukey revealed a reduction in pNF-H/M and MAP2 reactive areas (45% and 70%), GFAP+ cells (20%) and reduced MAP2 and pNF-H/M immunocontents in PA-rat striatum. PEA totally restored GFAP+ cells and MAP2 immunoreaction and partially prevented the decreased pNF-H/M reactive area (78%) induced by PA. PEA also reversed the MAP2 reduction and partially prevented the decrease of pNF-H/M immunocontent induced by PA. No GFAP level alteration was detected. PEA treatment attenuated the striatum damage induced by PA, demonstrating its therapeutic potential against PA.Fil: Udovin, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; ArgentinaFil: Kobiec, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Pontificia Universidad Católica de Argentina Santa María de los Buenos Aires. Facutlad de Piscología y Psicopedagogía. Centro de Investigaciones en Psicología y Pedagogía; ArgentinaFil: Herrera, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Pontificia Universidad Católica de Argentina Santa María de los Buenos Aires. Facutlad de Piscología y Psicopedagogía. Centro de Investigaciones en Psicología y Pedagogía; ArgentinaFil: Ramos Hryb, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Toro, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; ArgentinaFil: Kusnier, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; ArgentinaFil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; ArgentinaXXXIII Congreso de la Sociedad Argentina de Investigación en NeurocienciasCórdobaArgentinaSociedad Argentina de Investigación en NeurocienciasUniversidad Nacional de Córdoba2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/200769Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum; XXXIII Congreso de la Sociedad Argentina de Investigación en Neurociencias; Córdoba; Argentina; 2018; 197-197CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2018_Cordoba-UNC_libro-de-resumenes.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:47Zoai:ri.conicet.gov.ar:11336/200769instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:48.213CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum
title Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum
spellingShingle Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum
Udovin, Lucas
NEUROPROTECTION
STRIATUM
PALMITOYLEHANOLAMIDE palmitoylethanolamide
PERINATAL ASPHYXIA
title_short Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum
title_full Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum
title_fullStr Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum
title_full_unstemmed Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum
title_sort Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum
dc.creator.none.fl_str_mv Udovin, Lucas
Kobiec, Tamara
Herrera, María Inés
Ramos Hryb, Ana Belen
Toro, Nicolas
Kusnier, Carlos Federico
Capani, Francisco
author Udovin, Lucas
author_facet Udovin, Lucas
Kobiec, Tamara
Herrera, María Inés
Ramos Hryb, Ana Belen
Toro, Nicolas
Kusnier, Carlos Federico
Capani, Francisco
author_role author
author2 Kobiec, Tamara
Herrera, María Inés
Ramos Hryb, Ana Belen
Toro, Nicolas
Kusnier, Carlos Federico
Capani, Francisco
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv NEUROPROTECTION
STRIATUM
PALMITOYLEHANOLAMIDE palmitoylethanolamide
PERINATAL ASPHYXIA
topic NEUROPROTECTION
STRIATUM
PALMITOYLEHANOLAMIDE palmitoylethanolamide
PERINATAL ASPHYXIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Perinatal asphyxia (PA) caused by low O2 availability during birth is associated with brain damage, been the striatum one of the most affected areas. Palmitoylethanolamide (PEA) is a neuroprotective amide in brain injury models, including PA. However, its effects against PA requires deeper study in the striatum. Using Bjelke? model, full-term pregnant rats were rapidly decapitated, and uterus horns placed in a water bath (37°C,19 min). One hour after, rats were treated with PEA (10 mg/kg,s.c.) and later given to surrogate mothers. 30d animals were perfused and coronal striatum sections as well as subcellular fractions were collected for immunohistochemistry and blotting (n=3). Phosphorylated high/medium molecular weight neurofilaments (pNF-H/M), Microtubule-Associated Protein 2 (MAP2), and Glial fibrillary acidic protein (GFAP) were analysed. All procedures were previously approved by our Institution. 2-way ANOVA followed by Tukey revealed a reduction in pNF-H/M and MAP2 reactive areas (45% and 70%), GFAP+ cells (20%) and reduced MAP2 and pNF-H/M immunocontents in PA-rat striatum. PEA totally restored GFAP+ cells and MAP2 immunoreaction and partially prevented the decreased pNF-H/M reactive area (78%) induced by PA. PEA also reversed the MAP2 reduction and partially prevented the decrease of pNF-H/M immunocontent induced by PA. No GFAP level alteration was detected. PEA treatment attenuated the striatum damage induced by PA, demonstrating its therapeutic potential against PA.
Fil: Udovin, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; Argentina
Fil: Kobiec, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Pontificia Universidad Católica de Argentina Santa María de los Buenos Aires. Facutlad de Piscología y Psicopedagogía. Centro de Investigaciones en Psicología y Pedagogía; Argentina
Fil: Herrera, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Pontificia Universidad Católica de Argentina Santa María de los Buenos Aires. Facutlad de Piscología y Psicopedagogía. Centro de Investigaciones en Psicología y Pedagogía; Argentina
Fil: Ramos Hryb, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Toro, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; Argentina
Fil: Kusnier, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; Argentina
Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; Argentina
XXXIII Congreso de la Sociedad Argentina de Investigación en Neurociencias
Córdoba
Argentina
Sociedad Argentina de Investigación en Neurociencias
description Perinatal asphyxia (PA) caused by low O2 availability during birth is associated with brain damage, been the striatum one of the most affected areas. Palmitoylethanolamide (PEA) is a neuroprotective amide in brain injury models, including PA. However, its effects against PA requires deeper study in the striatum. Using Bjelke? model, full-term pregnant rats were rapidly decapitated, and uterus horns placed in a water bath (37°C,19 min). One hour after, rats were treated with PEA (10 mg/kg,s.c.) and later given to surrogate mothers. 30d animals were perfused and coronal striatum sections as well as subcellular fractions were collected for immunohistochemistry and blotting (n=3). Phosphorylated high/medium molecular weight neurofilaments (pNF-H/M), Microtubule-Associated Protein 2 (MAP2), and Glial fibrillary acidic protein (GFAP) were analysed. All procedures were previously approved by our Institution. 2-way ANOVA followed by Tukey revealed a reduction in pNF-H/M and MAP2 reactive areas (45% and 70%), GFAP+ cells (20%) and reduced MAP2 and pNF-H/M immunocontents in PA-rat striatum. PEA totally restored GFAP+ cells and MAP2 immunoreaction and partially prevented the decreased pNF-H/M reactive area (78%) induced by PA. PEA also reversed the MAP2 reduction and partially prevented the decrease of pNF-H/M immunocontent induced by PA. No GFAP level alteration was detected. PEA treatment attenuated the striatum damage induced by PA, demonstrating its therapeutic potential against PA.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/200769
Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum; XXXIII Congreso de la Sociedad Argentina de Investigación en Neurociencias; Córdoba; Argentina; 2018; 197-197
CONICET Digital
CONICET
url http://hdl.handle.net/11336/200769
identifier_str_mv Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: An analysis of the rat striatum; XXXIII Congreso de la Sociedad Argentina de Investigación en Neurociencias; Córdoba; Argentina; 2018; 197-197
CONICET Digital
CONICET
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language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad Nacional de Córdoba
publisher.none.fl_str_mv Universidad Nacional de Córdoba
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