Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring

Autores
Abate, Paula; Hernandez Fonseca, K.; Reyes Guzman, A. C.; Barbosa Luna, I. G.; Méndez Ubach, Milagros
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The endogenous opioid system is involved in ethanol reinforcement. Ethanol-induced changes in opioidergic transmission have been extensively studied in adult organisms. However, the impact of ethanol exposure at low or moderate doses during early ontogeny has been barely explored. We investigated the effect of prenatal ethanol exposure on alcohol intake and Methionine-enkephalin (Met-enk) content in rat offspring. Met-enk content was assessed in the ventral tegmental area [VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP], amygdala, hypothalamus and hippocampus. Pregnant rats were treated with ethanol (2 g/kg) or water during GDs 17–20. At PDs 14 and 15, preweanlings were evaluated in an intake test (5% and 10% ethanol, or water). Met-enk content in brain regions of infants prenatally exposed to ethanol was quantitated by radioimmunoassay. Ethanol consumption was facilitated by prenatal experience with the drug, particularly in females. Metenk content in mesocorticolimbic regions – PFC and NAcc – was increased as a consequence of prenatal exposure to ethanol. Conversely, Met-enk levels in the VTA were reduced by prenatal ethanol manipulation. Prenatal ethanol also increased peptide levels in the medial-posterior zone of the CP, and strongly augmented Met-enk content in the hippocampus and hypothalamus. These findings show that prenatal ethanol exposure stimulates consumption of the drug in infant rats, and induces selective changes in Met-enk levels in regions of the mesocorticolimbic and nigrostriatal systems, the hypothalamus and hippocampus. Our results support the role of mesocorticolimbic enkephalins in ethanol reinforcement in offspring, as has been reported in adults.
Fil: Abate, Paula. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Hernandez Fonseca, K.. Instituto Nacional de Psiquiatria Ramon de la Fuente. Departamento de Neuroquímica; México
Fil: Reyes Guzman, A. C.. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
Fil: Barbosa Luna, I. G.. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
Fil: Méndez Ubach, Milagros. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
Materia
ENKEPHALINS
ETHANOL
OFFSPRING
ONTOGENY
OPIOID PEPTIDES
REINFORCEMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/185729
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/185729
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspringAbate, PaulaHernandez Fonseca, K.Reyes Guzman, A. C.Barbosa Luna, I. G.Méndez Ubach, MilagrosENKEPHALINSETHANOLOFFSPRINGONTOGENYOPIOID PEPTIDESREINFORCEMENThttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The endogenous opioid system is involved in ethanol reinforcement. Ethanol-induced changes in opioidergic transmission have been extensively studied in adult organisms. However, the impact of ethanol exposure at low or moderate doses during early ontogeny has been barely explored. We investigated the effect of prenatal ethanol exposure on alcohol intake and Methionine-enkephalin (Met-enk) content in rat offspring. Met-enk content was assessed in the ventral tegmental area [VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP], amygdala, hypothalamus and hippocampus. Pregnant rats were treated with ethanol (2 g/kg) or water during GDs 17–20. At PDs 14 and 15, preweanlings were evaluated in an intake test (5% and 10% ethanol, or water). Met-enk content in brain regions of infants prenatally exposed to ethanol was quantitated by radioimmunoassay. Ethanol consumption was facilitated by prenatal experience with the drug, particularly in females. Metenk content in mesocorticolimbic regions – PFC and NAcc – was increased as a consequence of prenatal exposure to ethanol. Conversely, Met-enk levels in the VTA were reduced by prenatal ethanol manipulation. Prenatal ethanol also increased peptide levels in the medial-posterior zone of the CP, and strongly augmented Met-enk content in the hippocampus and hypothalamus. These findings show that prenatal ethanol exposure stimulates consumption of the drug in infant rats, and induces selective changes in Met-enk levels in regions of the mesocorticolimbic and nigrostriatal systems, the hypothalamus and hippocampus. Our results support the role of mesocorticolimbic enkephalins in ethanol reinforcement in offspring, as has been reported in adults.Fil: Abate, Paula. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Hernandez Fonseca, K.. Instituto Nacional de Psiquiatria Ramon de la Fuente. Departamento de Neuroquímica; MéxicoFil: Reyes Guzman, A. C.. Instituto Nacional de Psiquiatría Ramón de la Fuente; MéxicoFil: Barbosa Luna, I. G.. Instituto Nacional de Psiquiatría Ramón de la Fuente; MéxicoFil: Méndez Ubach, Milagros. Instituto Nacional de Psiquiatría Ramón de la Fuente; MéxicoElsevier Science2014-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/185729Abate, Paula; Hernandez Fonseca, K.; Reyes Guzman, A. C.; Barbosa Luna, I. G.; Méndez Ubach, Milagros; Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring; Elsevier Science; Behavioural Brain Research; 274; 1-11-2014; 194-2040166-43281872-7549CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166432814005348info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbr.2014.08.022info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:31Zoai:ri.conicet.gov.ar:11336/185729instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:32.06CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring
title Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring
spellingShingle Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring
Abate, Paula
ENKEPHALINS
ETHANOL
OFFSPRING
ONTOGENY
OPIOID PEPTIDES
REINFORCEMENT
title_short Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring
title_full Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring
title_fullStr Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring
title_full_unstemmed Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring
title_sort Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring
dc.creator.none.fl_str_mv Abate, Paula
Hernandez Fonseca, K.
Reyes Guzman, A. C.
Barbosa Luna, I. G.
Méndez Ubach, Milagros
author Abate, Paula
author_facet Abate, Paula
Hernandez Fonseca, K.
Reyes Guzman, A. C.
Barbosa Luna, I. G.
Méndez Ubach, Milagros
author_role author
author2 Hernandez Fonseca, K.
Reyes Guzman, A. C.
Barbosa Luna, I. G.
Méndez Ubach, Milagros
author2_role author
author
author
author
dc.subject.none.fl_str_mv ENKEPHALINS
ETHANOL
OFFSPRING
ONTOGENY
OPIOID PEPTIDES
REINFORCEMENT
topic ENKEPHALINS
ETHANOL
OFFSPRING
ONTOGENY
OPIOID PEPTIDES
REINFORCEMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The endogenous opioid system is involved in ethanol reinforcement. Ethanol-induced changes in opioidergic transmission have been extensively studied in adult organisms. However, the impact of ethanol exposure at low or moderate doses during early ontogeny has been barely explored. We investigated the effect of prenatal ethanol exposure on alcohol intake and Methionine-enkephalin (Met-enk) content in rat offspring. Met-enk content was assessed in the ventral tegmental area [VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP], amygdala, hypothalamus and hippocampus. Pregnant rats were treated with ethanol (2 g/kg) or water during GDs 17–20. At PDs 14 and 15, preweanlings were evaluated in an intake test (5% and 10% ethanol, or water). Met-enk content in brain regions of infants prenatally exposed to ethanol was quantitated by radioimmunoassay. Ethanol consumption was facilitated by prenatal experience with the drug, particularly in females. Metenk content in mesocorticolimbic regions – PFC and NAcc – was increased as a consequence of prenatal exposure to ethanol. Conversely, Met-enk levels in the VTA were reduced by prenatal ethanol manipulation. Prenatal ethanol also increased peptide levels in the medial-posterior zone of the CP, and strongly augmented Met-enk content in the hippocampus and hypothalamus. These findings show that prenatal ethanol exposure stimulates consumption of the drug in infant rats, and induces selective changes in Met-enk levels in regions of the mesocorticolimbic and nigrostriatal systems, the hypothalamus and hippocampus. Our results support the role of mesocorticolimbic enkephalins in ethanol reinforcement in offspring, as has been reported in adults.
Fil: Abate, Paula. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Hernandez Fonseca, K.. Instituto Nacional de Psiquiatria Ramon de la Fuente. Departamento de Neuroquímica; México
Fil: Reyes Guzman, A. C.. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
Fil: Barbosa Luna, I. G.. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
Fil: Méndez Ubach, Milagros. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
description The endogenous opioid system is involved in ethanol reinforcement. Ethanol-induced changes in opioidergic transmission have been extensively studied in adult organisms. However, the impact of ethanol exposure at low or moderate doses during early ontogeny has been barely explored. We investigated the effect of prenatal ethanol exposure on alcohol intake and Methionine-enkephalin (Met-enk) content in rat offspring. Met-enk content was assessed in the ventral tegmental area [VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP], amygdala, hypothalamus and hippocampus. Pregnant rats were treated with ethanol (2 g/kg) or water during GDs 17–20. At PDs 14 and 15, preweanlings were evaluated in an intake test (5% and 10% ethanol, or water). Met-enk content in brain regions of infants prenatally exposed to ethanol was quantitated by radioimmunoassay. Ethanol consumption was facilitated by prenatal experience with the drug, particularly in females. Metenk content in mesocorticolimbic regions – PFC and NAcc – was increased as a consequence of prenatal exposure to ethanol. Conversely, Met-enk levels in the VTA were reduced by prenatal ethanol manipulation. Prenatal ethanol also increased peptide levels in the medial-posterior zone of the CP, and strongly augmented Met-enk content in the hippocampus and hypothalamus. These findings show that prenatal ethanol exposure stimulates consumption of the drug in infant rats, and induces selective changes in Met-enk levels in regions of the mesocorticolimbic and nigrostriatal systems, the hypothalamus and hippocampus. Our results support the role of mesocorticolimbic enkephalins in ethanol reinforcement in offspring, as has been reported in adults.
publishDate 2014
dc.date.none.fl_str_mv 2014-11-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/185729
Abate, Paula; Hernandez Fonseca, K.; Reyes Guzman, A. C.; Barbosa Luna, I. G.; Méndez Ubach, Milagros; Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring; Elsevier Science; Behavioural Brain Research; 274; 1-11-2014; 194-204
0166-4328
1872-7549
CONICET Digital
CONICET
url http://hdl.handle.net/11336/185729
identifier_str_mv Abate, Paula; Hernandez Fonseca, K.; Reyes Guzman, A. C.; Barbosa Luna, I. G.; Méndez Ubach, Milagros; Prenatal ethanol exposure alters met-enkephalin expression in brain regions related with reinforcement: Possible mechanism for ethanol consumption in offspring; Elsevier Science; Behavioural Brain Research; 274; 1-11-2014; 194-204
0166-4328
1872-7549
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166432814005348
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbr.2014.08.022
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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