Interferon-β gene transfer inhibits melanoma cells adhesion and migration

Autores
Rossi, Úrsula A.; Finocchiaro, Liliana Maria Elena; Glikin, Gerardo Claudio
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We evaluated the effects of expression of interferon-β (IFNβ) after lipofection on melanoma cells adhesion and migration. Three canine mucosal (Ak, Br and Ol) and one human dermal (SB2) melanomas were assayed. By means of the wound healing assay, we found a significant inhibitory effect of canine IFNβ gene expression on cells migration in Br and Ol monolayers. This effect could be reproduced on unlipofected Ol cells with conditioned culture media obtained from canine IFNβ gene-lipofected Ol cells, and with recombinant human IFNβ on unlipofected SB2 cells. Furthermore, IFNβ gene expression of the four tested tumor cells significantly inhibited their adhesion to extracellular matrix (ECM) proteins and their spreading from multicellular spheroids onto gelatin coating. The addition of catalase reverted the increase of reactive oxygen species (ROS) in Ol cells and the inhibition of cell migration in monolayers (Ol) and spheroids (Ol an SB2) produced by canine and human IFNβ expression, suggesting the involvement of ROS as mediators of IFNβ action on the cells interactions with ECM. Together with its known immune, antiangiogenic and cytotoxic effects, the present data strongly support more studies exploring the clinical potential of IFNβ for cancer therapy.
Fil: Rossi, Úrsula A.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina
Fil: Finocchiaro, Liliana Maria Elena. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Glikin, Gerardo Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina
Materia
Canine
Dmrie
Human
Lipofection
Ros
Spheroids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38771
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38771
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interferon-β gene transfer inhibits melanoma cells adhesion and migrationRossi, Úrsula A.Finocchiaro, Liliana Maria ElenaGlikin, Gerardo ClaudioCanineDmrieHumanLipofectionRosSpheroidshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We evaluated the effects of expression of interferon-β (IFNβ) after lipofection on melanoma cells adhesion and migration. Three canine mucosal (Ak, Br and Ol) and one human dermal (SB2) melanomas were assayed. By means of the wound healing assay, we found a significant inhibitory effect of canine IFNβ gene expression on cells migration in Br and Ol monolayers. This effect could be reproduced on unlipofected Ol cells with conditioned culture media obtained from canine IFNβ gene-lipofected Ol cells, and with recombinant human IFNβ on unlipofected SB2 cells. Furthermore, IFNβ gene expression of the four tested tumor cells significantly inhibited their adhesion to extracellular matrix (ECM) proteins and their spreading from multicellular spheroids onto gelatin coating. The addition of catalase reverted the increase of reactive oxygen species (ROS) in Ol cells and the inhibition of cell migration in monolayers (Ol) and spheroids (Ol an SB2) produced by canine and human IFNβ expression, suggesting the involvement of ROS as mediators of IFNβ action on the cells interactions with ECM. Together with its known immune, antiangiogenic and cytotoxic effects, the present data strongly support more studies exploring the clinical potential of IFNβ for cancer therapy.Fil: Rossi, Úrsula A.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; ArgentinaFil: Finocchiaro, Liliana Maria Elena. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Glikin, Gerardo Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38771Rossi, Úrsula A.; Finocchiaro, Liliana Maria Elena; Glikin, Gerardo Claudio; Interferon-β gene transfer inhibits melanoma cells adhesion and migration; Academic Press Ltd - Elsevier Science Ltd; Cytokine; 89; 1-2017; 201-2081043-4666CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1043466615301009info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cyto.2015.11.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:59Zoai:ri.conicet.gov.ar:11336/38771instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:59.591CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interferon-β gene transfer inhibits melanoma cells adhesion and migration
title Interferon-β gene transfer inhibits melanoma cells adhesion and migration
spellingShingle Interferon-β gene transfer inhibits melanoma cells adhesion and migration
Rossi, Úrsula A.
Canine
Dmrie
Human
Lipofection
Ros
Spheroids
title_short Interferon-β gene transfer inhibits melanoma cells adhesion and migration
title_full Interferon-β gene transfer inhibits melanoma cells adhesion and migration
title_fullStr Interferon-β gene transfer inhibits melanoma cells adhesion and migration
title_full_unstemmed Interferon-β gene transfer inhibits melanoma cells adhesion and migration
title_sort Interferon-β gene transfer inhibits melanoma cells adhesion and migration
dc.creator.none.fl_str_mv Rossi, Úrsula A.
Finocchiaro, Liliana Maria Elena
Glikin, Gerardo Claudio
author Rossi, Úrsula A.
author_facet Rossi, Úrsula A.
Finocchiaro, Liliana Maria Elena
Glikin, Gerardo Claudio
author_role author
author2 Finocchiaro, Liliana Maria Elena
Glikin, Gerardo Claudio
author2_role author
author
dc.subject.none.fl_str_mv Canine
Dmrie
Human
Lipofection
Ros
Spheroids
topic Canine
Dmrie
Human
Lipofection
Ros
Spheroids
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We evaluated the effects of expression of interferon-β (IFNβ) after lipofection on melanoma cells adhesion and migration. Three canine mucosal (Ak, Br and Ol) and one human dermal (SB2) melanomas were assayed. By means of the wound healing assay, we found a significant inhibitory effect of canine IFNβ gene expression on cells migration in Br and Ol monolayers. This effect could be reproduced on unlipofected Ol cells with conditioned culture media obtained from canine IFNβ gene-lipofected Ol cells, and with recombinant human IFNβ on unlipofected SB2 cells. Furthermore, IFNβ gene expression of the four tested tumor cells significantly inhibited their adhesion to extracellular matrix (ECM) proteins and their spreading from multicellular spheroids onto gelatin coating. The addition of catalase reverted the increase of reactive oxygen species (ROS) in Ol cells and the inhibition of cell migration in monolayers (Ol) and spheroids (Ol an SB2) produced by canine and human IFNβ expression, suggesting the involvement of ROS as mediators of IFNβ action on the cells interactions with ECM. Together with its known immune, antiangiogenic and cytotoxic effects, the present data strongly support more studies exploring the clinical potential of IFNβ for cancer therapy.
Fil: Rossi, Úrsula A.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina
Fil: Finocchiaro, Liliana Maria Elena. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Glikin, Gerardo Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina
description We evaluated the effects of expression of interferon-β (IFNβ) after lipofection on melanoma cells adhesion and migration. Three canine mucosal (Ak, Br and Ol) and one human dermal (SB2) melanomas were assayed. By means of the wound healing assay, we found a significant inhibitory effect of canine IFNβ gene expression on cells migration in Br and Ol monolayers. This effect could be reproduced on unlipofected Ol cells with conditioned culture media obtained from canine IFNβ gene-lipofected Ol cells, and with recombinant human IFNβ on unlipofected SB2 cells. Furthermore, IFNβ gene expression of the four tested tumor cells significantly inhibited their adhesion to extracellular matrix (ECM) proteins and their spreading from multicellular spheroids onto gelatin coating. The addition of catalase reverted the increase of reactive oxygen species (ROS) in Ol cells and the inhibition of cell migration in monolayers (Ol) and spheroids (Ol an SB2) produced by canine and human IFNβ expression, suggesting the involvement of ROS as mediators of IFNβ action on the cells interactions with ECM. Together with its known immune, antiangiogenic and cytotoxic effects, the present data strongly support more studies exploring the clinical potential of IFNβ for cancer therapy.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38771
Rossi, Úrsula A.; Finocchiaro, Liliana Maria Elena; Glikin, Gerardo Claudio; Interferon-β gene transfer inhibits melanoma cells adhesion and migration; Academic Press Ltd - Elsevier Science Ltd; Cytokine; 89; 1-2017; 201-208
1043-4666
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38771
identifier_str_mv Rossi, Úrsula A.; Finocchiaro, Liliana Maria Elena; Glikin, Gerardo Claudio; Interferon-β gene transfer inhibits melanoma cells adhesion and migration; Academic Press Ltd - Elsevier Science Ltd; Cytokine; 89; 1-2017; 201-208
1043-4666
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1043466615301009
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cyto.2015.11.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613048683200512
score 13.070432

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