Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135
- Autores
- Colin, Veronica Leticia; Baigori, Mario Domingo; Pera, Licia Maria
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Introduction: Lipases (EC 3.1.1.3) are enzymes that hydrolyse the ester bonds of water insoluble substrates at the interface between the substrate and the water. This reaction is reversible. Lipases may also catalyze ester synthesis and transesterification in reaction mixtures with low water contents. Objective: The aim of this work was to study the influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135. Materials and methods: The fermentation medium comprised (in g/l): sucrose 10; NH4NO3 2; KH2PO4 1; MgSO4.7H2O 0.2; CuSO4.5H2O 0.06. The effect of modification in the environmental condition on lipase production was tested by changing the initial pH of the medium as well as by the addition of CaCl2, FeCl3 or Tween (20, 40, 60, and 80). Lipase activity was determined using 0.01 g of wet mycelium and p-nitrophenyl palmitate as substrate. Results and conclusions: The assayed culture conditions scientifically influences the lipase production. The highest specific activity (36.6 mU/g of DW) was obtained with either initial pH 8 or in presence of 0.5% Tween 60. This work was supported by grants PIP 6062 and PICTO 761.
Fil: Colin, Veronica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XXIII Jornadas Científicas de la Asociación de Biología de Tucumán
Tafí del Valle
Argentina
Asociación de Biología de Tucumán - Materia
-
LIPASE PRODUCTION
FILAMENTOUS FUNGI
CULTURE CONDITIONS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/201030
Ver los metadatos del registro completo
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Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135Colin, Veronica LeticiaBaigori, Mario DomingoPera, Licia MariaLIPASE PRODUCTIONFILAMENTOUS FUNGICULTURE CONDITIONShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Introduction: Lipases (EC 3.1.1.3) are enzymes that hydrolyse the ester bonds of water insoluble substrates at the interface between the substrate and the water. This reaction is reversible. Lipases may also catalyze ester synthesis and transesterification in reaction mixtures with low water contents. Objective: The aim of this work was to study the influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135. Materials and methods: The fermentation medium comprised (in g/l): sucrose 10; NH4NO3 2; KH2PO4 1; MgSO4.7H2O 0.2; CuSO4.5H2O 0.06. The effect of modification in the environmental condition on lipase production was tested by changing the initial pH of the medium as well as by the addition of CaCl2, FeCl3 or Tween (20, 40, 60, and 80). Lipase activity was determined using 0.01 g of wet mycelium and p-nitrophenyl palmitate as substrate. Results and conclusions: The assayed culture conditions scientifically influences the lipase production. The highest specific activity (36.6 mU/g of DW) was obtained with either initial pH 8 or in presence of 0.5% Tween 60. This work was supported by grants PIP 6062 and PICTO 761.Fil: Colin, Veronica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXXIII Jornadas Científicas de la Asociación de Biología de TucumánTafí del ValleArgentinaAsociación de Biología de TucumánTech Science Press2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201030Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135; XXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2006; 289-2890327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.asobioltuc.com/uploads/archivos/1503955682_QmlvY2VsbCBYWElJSSBKQyAyMDA2LnBkZg==.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:02:55Zoai:ri.conicet.gov.ar:11336/201030instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:02:55.957CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135 |
title |
Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135 |
spellingShingle |
Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135 Colin, Veronica Leticia LIPASE PRODUCTION FILAMENTOUS FUNGI CULTURE CONDITIONS |
title_short |
Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135 |
title_full |
Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135 |
title_fullStr |
Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135 |
title_full_unstemmed |
Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135 |
title_sort |
Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135 |
dc.creator.none.fl_str_mv |
Colin, Veronica Leticia Baigori, Mario Domingo Pera, Licia Maria |
author |
Colin, Veronica Leticia |
author_facet |
Colin, Veronica Leticia Baigori, Mario Domingo Pera, Licia Maria |
author_role |
author |
author2 |
Baigori, Mario Domingo Pera, Licia Maria |
author2_role |
author author |
dc.subject.none.fl_str_mv |
LIPASE PRODUCTION FILAMENTOUS FUNGI CULTURE CONDITIONS |
topic |
LIPASE PRODUCTION FILAMENTOUS FUNGI CULTURE CONDITIONS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Introduction: Lipases (EC 3.1.1.3) are enzymes that hydrolyse the ester bonds of water insoluble substrates at the interface between the substrate and the water. This reaction is reversible. Lipases may also catalyze ester synthesis and transesterification in reaction mixtures with low water contents. Objective: The aim of this work was to study the influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135. Materials and methods: The fermentation medium comprised (in g/l): sucrose 10; NH4NO3 2; KH2PO4 1; MgSO4.7H2O 0.2; CuSO4.5H2O 0.06. The effect of modification in the environmental condition on lipase production was tested by changing the initial pH of the medium as well as by the addition of CaCl2, FeCl3 or Tween (20, 40, 60, and 80). Lipase activity was determined using 0.01 g of wet mycelium and p-nitrophenyl palmitate as substrate. Results and conclusions: The assayed culture conditions scientifically influences the lipase production. The highest specific activity (36.6 mU/g of DW) was obtained with either initial pH 8 or in presence of 0.5% Tween 60. This work was supported by grants PIP 6062 and PICTO 761. Fil: Colin, Veronica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina XXIII Jornadas Científicas de la Asociación de Biología de Tucumán Tafí del Valle Argentina Asociación de Biología de Tucumán |
description |
Introduction: Lipases (EC 3.1.1.3) are enzymes that hydrolyse the ester bonds of water insoluble substrates at the interface between the substrate and the water. This reaction is reversible. Lipases may also catalyze ester synthesis and transesterification in reaction mixtures with low water contents. Objective: The aim of this work was to study the influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135. Materials and methods: The fermentation medium comprised (in g/l): sucrose 10; NH4NO3 2; KH2PO4 1; MgSO4.7H2O 0.2; CuSO4.5H2O 0.06. The effect of modification in the environmental condition on lipase production was tested by changing the initial pH of the medium as well as by the addition of CaCl2, FeCl3 or Tween (20, 40, 60, and 80). Lipase activity was determined using 0.01 g of wet mycelium and p-nitrophenyl palmitate as substrate. Results and conclusions: The assayed culture conditions scientifically influences the lipase production. The highest specific activity (36.6 mU/g of DW) was obtained with either initial pH 8 or in presence of 0.5% Tween 60. This work was supported by grants PIP 6062 and PICTO 761. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Jornada Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/201030 Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135; XXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2006; 289-289 0327-9545 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/201030 |
identifier_str_mv |
Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135; XXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2006; 289-289 0327-9545 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.asobioltuc.com/uploads/archivos/1503955682_QmlvY2VsbCBYWElJSSBKQyAyMDA2LnBkZg==.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Tech Science Press |
publisher.none.fl_str_mv |
Tech Science Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083171200794624 |
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13.22299 |