Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135

Autores
Colin, Veronica Leticia; Baigori, Mario Domingo; Pera, Licia Maria
Año de publicación
2007
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Introduction: Lipases (EC 3.1.1.3) are enzymes that hydrolyse the ester bonds of water insoluble substrates at the interface between the substrate and the water. This reaction is reversible. Lipases may also catalyze ester synthesis and transesterification in reaction mixtures with low water contents. Objective: The aim of this work was to study the influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135. Materials and methods: The fermentation medium comprised (in g/l): sucrose 10; NH4NO3 2; KH2PO4 1; MgSO4.7H2O 0.2; CuSO4.5H2O 0.06. The effect of modification in the environmental condition on lipase production was tested by changing the initial pH of the medium as well as by the addition of CaCl2, FeCl3 or Tween (20, 40, 60, and 80). Lipase activity was determined using 0.01 g of wet mycelium and p-nitrophenyl palmitate as substrate. Results and conclusions: The assayed culture conditions scientifically influences the lipase production. The highest specific activity (36.6 mU/g of DW) was obtained with either initial pH 8 or in presence of 0.5% Tween 60. This work was supported by grants PIP 6062 and PICTO 761.
Fil: Colin, Veronica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XXIII Jornadas Científicas de la Asociación de Biología de Tucumán
Tafí del Valle
Argentina
Asociación de Biología de Tucumán
Materia
LIPASE PRODUCTION
FILAMENTOUS FUNGI
CULTURE CONDITIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201030

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network_name_str CONICET Digital (CONICET)
spelling Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135Colin, Veronica LeticiaBaigori, Mario DomingoPera, Licia MariaLIPASE PRODUCTIONFILAMENTOUS FUNGICULTURE CONDITIONShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Introduction: Lipases (EC 3.1.1.3) are enzymes that hydrolyse the ester bonds of water insoluble substrates at the interface between the substrate and the water. This reaction is reversible. Lipases may also catalyze ester synthesis and transesterification in reaction mixtures with low water contents. Objective: The aim of this work was to study the influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135. Materials and methods: The fermentation medium comprised (in g/l): sucrose 10; NH4NO3 2; KH2PO4 1; MgSO4.7H2O 0.2; CuSO4.5H2O 0.06. The effect of modification in the environmental condition on lipase production was tested by changing the initial pH of the medium as well as by the addition of CaCl2, FeCl3 or Tween (20, 40, 60, and 80). Lipase activity was determined using 0.01 g of wet mycelium and p-nitrophenyl palmitate as substrate. Results and conclusions: The assayed culture conditions scientifically influences the lipase production. The highest specific activity (36.6 mU/g of DW) was obtained with either initial pH 8 or in presence of 0.5% Tween 60. This work was supported by grants PIP 6062 and PICTO 761.Fil: Colin, Veronica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXXIII Jornadas Científicas de la Asociación de Biología de TucumánTafí del ValleArgentinaAsociación de Biología de TucumánTech Science Press2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201030Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135; XXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2006; 289-2890327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.asobioltuc.com/uploads/archivos/1503955682_QmlvY2VsbCBYWElJSSBKQyAyMDA2LnBkZg==.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:02:55Zoai:ri.conicet.gov.ar:11336/201030instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:02:55.957CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135
title Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135
spellingShingle Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135
Colin, Veronica Leticia
LIPASE PRODUCTION
FILAMENTOUS FUNGI
CULTURE CONDITIONS
title_short Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135
title_full Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135
title_fullStr Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135
title_full_unstemmed Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135
title_sort Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135
dc.creator.none.fl_str_mv Colin, Veronica Leticia
Baigori, Mario Domingo
Pera, Licia Maria
author Colin, Veronica Leticia
author_facet Colin, Veronica Leticia
Baigori, Mario Domingo
Pera, Licia Maria
author_role author
author2 Baigori, Mario Domingo
Pera, Licia Maria
author2_role author
author
dc.subject.none.fl_str_mv LIPASE PRODUCTION
FILAMENTOUS FUNGI
CULTURE CONDITIONS
topic LIPASE PRODUCTION
FILAMENTOUS FUNGI
CULTURE CONDITIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Introduction: Lipases (EC 3.1.1.3) are enzymes that hydrolyse the ester bonds of water insoluble substrates at the interface between the substrate and the water. This reaction is reversible. Lipases may also catalyze ester synthesis and transesterification in reaction mixtures with low water contents. Objective: The aim of this work was to study the influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135. Materials and methods: The fermentation medium comprised (in g/l): sucrose 10; NH4NO3 2; KH2PO4 1; MgSO4.7H2O 0.2; CuSO4.5H2O 0.06. The effect of modification in the environmental condition on lipase production was tested by changing the initial pH of the medium as well as by the addition of CaCl2, FeCl3 or Tween (20, 40, 60, and 80). Lipase activity was determined using 0.01 g of wet mycelium and p-nitrophenyl palmitate as substrate. Results and conclusions: The assayed culture conditions scientifically influences the lipase production. The highest specific activity (36.6 mU/g of DW) was obtained with either initial pH 8 or in presence of 0.5% Tween 60. This work was supported by grants PIP 6062 and PICTO 761.
Fil: Colin, Veronica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XXIII Jornadas Científicas de la Asociación de Biología de Tucumán
Tafí del Valle
Argentina
Asociación de Biología de Tucumán
description Introduction: Lipases (EC 3.1.1.3) are enzymes that hydrolyse the ester bonds of water insoluble substrates at the interface between the substrate and the water. This reaction is reversible. Lipases may also catalyze ester synthesis and transesterification in reaction mixtures with low water contents. Objective: The aim of this work was to study the influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135. Materials and methods: The fermentation medium comprised (in g/l): sucrose 10; NH4NO3 2; KH2PO4 1; MgSO4.7H2O 0.2; CuSO4.5H2O 0.06. The effect of modification in the environmental condition on lipase production was tested by changing the initial pH of the medium as well as by the addition of CaCl2, FeCl3 or Tween (20, 40, 60, and 80). Lipase activity was determined using 0.01 g of wet mycelium and p-nitrophenyl palmitate as substrate. Results and conclusions: The assayed culture conditions scientifically influences the lipase production. The highest specific activity (36.6 mU/g of DW) was obtained with either initial pH 8 or in presence of 0.5% Tween 60. This work was supported by grants PIP 6062 and PICTO 761.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201030
Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135; XXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2006; 289-289
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201030
identifier_str_mv Influence of culture conditions on a mycelium-bound lipase production from Aspergillus niger MYA 135; XXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2006; 289-289
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
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application/pdf
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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