Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine

Autores
Plaul, Silvia Elena; Pastor, Raquel; Díaz, Alcira Ofelia; Barbeito, Claudio Gustavo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Neotropical catfish, Corydoras paleatus (Callichthyidae) is a facultative air-breathing teleost that makes use of the caudal portion of the intestine as an accessory air-breathing organ. This portion is highly modified, being well vascularized with capillaries between epithelial cells, which makes it well suited for gas exchange. Instead, the cranial portion is a digestion and absorption site, as it has a typical intestinal epithelium with columnar cells arranged in a single row, villi and less vascularized tunica mucosa. Therefore, the intestine was studied by light and electron microscopy to assess differences between the cranial, middle and caudal portions. To characterize the potential for cell proliferation of this organ, we used anti-proliferating cell nuclear antigen antibody and anti-Na+K+-ATPase monoclonal antibody to detect the presence of Na+/K+ pump. In C. paleatus it was observed that cell dynamics showed a decreasing gradient of proliferation in cranio-caudal direction. Also, the intestine of this catfish is an important organ in ionoregulation: the basolateral Na+/K+ pump may have an active role, transporting Na+ out of the cell while helping to maintain the repose potential and to regulate cellular volume.
Fil: Plaul, Silvia Elena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pastor, Raquel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Díaz, Alcira Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Air-Breathing Organ
Na+K+-Atpase
Neotropical Catfish
Pcna
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54300
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestinePlaul, Silvia ElenaPastor, RaquelDíaz, Alcira OfeliaBarbeito, Claudio GustavoAir-Breathing OrganNa+K+-AtpaseNeotropical CatfishPcnahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Neotropical catfish, Corydoras paleatus (Callichthyidae) is a facultative air-breathing teleost that makes use of the caudal portion of the intestine as an accessory air-breathing organ. This portion is highly modified, being well vascularized with capillaries between epithelial cells, which makes it well suited for gas exchange. Instead, the cranial portion is a digestion and absorption site, as it has a typical intestinal epithelium with columnar cells arranged in a single row, villi and less vascularized tunica mucosa. Therefore, the intestine was studied by light and electron microscopy to assess differences between the cranial, middle and caudal portions. To characterize the potential for cell proliferation of this organ, we used anti-proliferating cell nuclear antigen antibody and anti-Na+K+-ATPase monoclonal antibody to detect the presence of Na+/K+ pump. In C. paleatus it was observed that cell dynamics showed a decreasing gradient of proliferation in cranio-caudal direction. Also, the intestine of this catfish is an important organ in ionoregulation: the basolateral Na+/K+ pump may have an active role, transporting Na+ out of the cell while helping to maintain the repose potential and to regulate cellular volume.Fil: Plaul, Silvia Elena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pastor, Raquel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Díaz, Alcira Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley-liss, Div John Wiley & Sons Inc2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54300Plaul, Silvia Elena; Pastor, Raquel; Díaz, Alcira Ofelia; Barbeito, Claudio Gustavo; Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine; Wiley-liss, Div John Wiley & Sons Inc; Microscopy Research And Technique.; 79; 3; 3-2016; 140-1481059-910XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jemt.22614info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jemt.22614info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:01:15Zoai:ri.conicet.gov.ar:11336/54300instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:01:15.525CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine
title Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine
spellingShingle Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine
Plaul, Silvia Elena
Air-Breathing Organ
Na+K+-Atpase
Neotropical Catfish
Pcna
title_short Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine
title_full Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine
title_fullStr Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine
title_full_unstemmed Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine
title_sort Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine
dc.creator.none.fl_str_mv Plaul, Silvia Elena
Pastor, Raquel
Díaz, Alcira Ofelia
Barbeito, Claudio Gustavo
author Plaul, Silvia Elena
author_facet Plaul, Silvia Elena
Pastor, Raquel
Díaz, Alcira Ofelia
Barbeito, Claudio Gustavo
author_role author
author2 Pastor, Raquel
Díaz, Alcira Ofelia
Barbeito, Claudio Gustavo
author2_role author
author
author
dc.subject.none.fl_str_mv Air-Breathing Organ
Na+K+-Atpase
Neotropical Catfish
Pcna
topic Air-Breathing Organ
Na+K+-Atpase
Neotropical Catfish
Pcna
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Neotropical catfish, Corydoras paleatus (Callichthyidae) is a facultative air-breathing teleost that makes use of the caudal portion of the intestine as an accessory air-breathing organ. This portion is highly modified, being well vascularized with capillaries between epithelial cells, which makes it well suited for gas exchange. Instead, the cranial portion is a digestion and absorption site, as it has a typical intestinal epithelium with columnar cells arranged in a single row, villi and less vascularized tunica mucosa. Therefore, the intestine was studied by light and electron microscopy to assess differences between the cranial, middle and caudal portions. To characterize the potential for cell proliferation of this organ, we used anti-proliferating cell nuclear antigen antibody and anti-Na+K+-ATPase monoclonal antibody to detect the presence of Na+/K+ pump. In C. paleatus it was observed that cell dynamics showed a decreasing gradient of proliferation in cranio-caudal direction. Also, the intestine of this catfish is an important organ in ionoregulation: the basolateral Na+/K+ pump may have an active role, transporting Na+ out of the cell while helping to maintain the repose potential and to regulate cellular volume.
Fil: Plaul, Silvia Elena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pastor, Raquel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Díaz, Alcira Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The Neotropical catfish, Corydoras paleatus (Callichthyidae) is a facultative air-breathing teleost that makes use of the caudal portion of the intestine as an accessory air-breathing organ. This portion is highly modified, being well vascularized with capillaries between epithelial cells, which makes it well suited for gas exchange. Instead, the cranial portion is a digestion and absorption site, as it has a typical intestinal epithelium with columnar cells arranged in a single row, villi and less vascularized tunica mucosa. Therefore, the intestine was studied by light and electron microscopy to assess differences between the cranial, middle and caudal portions. To characterize the potential for cell proliferation of this organ, we used anti-proliferating cell nuclear antigen antibody and anti-Na+K+-ATPase monoclonal antibody to detect the presence of Na+/K+ pump. In C. paleatus it was observed that cell dynamics showed a decreasing gradient of proliferation in cranio-caudal direction. Also, the intestine of this catfish is an important organ in ionoregulation: the basolateral Na+/K+ pump may have an active role, transporting Na+ out of the cell while helping to maintain the repose potential and to regulate cellular volume.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54300
Plaul, Silvia Elena; Pastor, Raquel; Díaz, Alcira Ofelia; Barbeito, Claudio Gustavo; Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine; Wiley-liss, Div John Wiley & Sons Inc; Microscopy Research And Technique.; 79; 3; 3-2016; 140-148
1059-910X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54300
identifier_str_mv Plaul, Silvia Elena; Pastor, Raquel; Díaz, Alcira Ofelia; Barbeito, Claudio Gustavo; Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine; Wiley-liss, Div John Wiley & Sons Inc; Microscopy Research And Technique.; 79; 3; 3-2016; 140-148
1059-910X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jemt.22614
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jemt.22614
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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