Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans

Autores
Franco, Diana Lorena; Canessa, Paulo; Bellora, Nicolás; Risau Gusman, Sebastian Luis; Olivares Yañez, Consuelo; Pérez Lara, Rodrigo; Libkind Frati, Diego; Larrondo, Luis F.; Marpegan, Luciano
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Circadian systems enable organisms to synchronize their physiology to daily and seasonal environmental changes relying on endogenous pacemakers that oscillate with a period close to 24 h even in the absence of external timing cues. The oscillations are achieved by intracellular transcriptional/translational feedback loops thoroughly characterized for many organisms, but still little is known about the presence and characteristics of circadian clocks in fungi other than Neurospora crassa. We sought to characterize the circadian system of a natural isolate of Aureobasidium pullulans, a cold-adapted yeast bearing great biotechnological potential. A. pullulans formed daily concentric rings that were synchronized by light/dark cycles and were also formed in constant darkness with a period of 24.5 h. Moreover, these rhythms were temperature compensated, as evidenced by experiments conducted at temperatures as low as 10 °C. Finally, the expression of clock-essential genes, frequency, white collar-1, white collar-2 and vivid was confirmed. In summary, our results indicate the existence of a functional circadian clock in A. pullulans, capable of sustaining rhythms at very low temperatures and, based on the presence of conserved clock-gene homologues, suggest a molecular and functional relationship to well-described circadian systems.
Fil: Franco, Diana Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Canessa, Paulo. Universidad Andrés Bello; Chile. Millennium Nucleus for Fungal Integrative and Synthetic Biology; Chile
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Risau Gusman, Sebastian Luis. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olivares Yañez, Consuelo. Pontificia Universidad Católica de Chile; Chile. Millennium Nucleus for Fungal Integrative and Synthetic Biology; Chile
Fil: Pérez Lara, Rodrigo. Pontificia Universidad Católica de Chile; Chile. Millennium Nucleus for Fungal Integrative and Synthetic Biology; Chile
Fil: Libkind Frati, Diego. Pontificia Universidad Católica de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Larrondo, Luis F.. Millennium Nucleus for Fungal Integrative and Synthetic Biology; Chile
Fil: Marpegan, Luciano. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Circadian Rhythms
Fungi
Bioinformatics
Oscillatory systems
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34270
Acceder
id CONICETDig_371acc385cbb9380ab88e5d5e49771cc
oai_identifier_str oai:ri.conicet.gov.ar:11336/34270
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulansFranco, Diana LorenaCanessa, PauloBellora, NicolásRisau Gusman, Sebastian LuisOlivares Yañez, ConsueloPérez Lara, RodrigoLibkind Frati, DiegoLarrondo, Luis F.Marpegan, LucianoCircadian RhythmsFungiBioinformaticsOscillatory systemshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Circadian systems enable organisms to synchronize their physiology to daily and seasonal environmental changes relying on endogenous pacemakers that oscillate with a period close to 24 h even in the absence of external timing cues. The oscillations are achieved by intracellular transcriptional/translational feedback loops thoroughly characterized for many organisms, but still little is known about the presence and characteristics of circadian clocks in fungi other than Neurospora crassa. We sought to characterize the circadian system of a natural isolate of Aureobasidium pullulans, a cold-adapted yeast bearing great biotechnological potential. A. pullulans formed daily concentric rings that were synchronized by light/dark cycles and were also formed in constant darkness with a period of 24.5 h. Moreover, these rhythms were temperature compensated, as evidenced by experiments conducted at temperatures as low as 10 °C. Finally, the expression of clock-essential genes, frequency, white collar-1, white collar-2 and vivid was confirmed. In summary, our results indicate the existence of a functional circadian clock in A. pullulans, capable of sustaining rhythms at very low temperatures and, based on the presence of conserved clock-gene homologues, suggest a molecular and functional relationship to well-described circadian systems.Fil: Franco, Diana Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Canessa, Paulo. Universidad Andrés Bello; Chile. Millennium Nucleus for Fungal Integrative and Synthetic Biology; ChileFil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Risau Gusman, Sebastian Luis. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Olivares Yañez, Consuelo. Pontificia Universidad Católica de Chile; Chile. Millennium Nucleus for Fungal Integrative and Synthetic Biology; ChileFil: Pérez Lara, Rodrigo. Pontificia Universidad Católica de Chile; Chile. Millennium Nucleus for Fungal Integrative and Synthetic Biology; ChileFil: Libkind Frati, Diego. Pontificia Universidad Católica de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Larrondo, Luis F.. Millennium Nucleus for Fungal Integrative and Synthetic Biology; ChileFil: Marpegan, Luciano. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaNature Publishing Group2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34270Franco, Diana Lorena; Canessa, Paulo; Bellora, Nicolás; Risau Gusman, Sebastian Luis; Olivares Yañez, Consuelo; et al.; Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans; Nature Publishing Group; Scientific Reports; 7; 1; 10-2017; 1-12; 138372045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-017-14085-6info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-14085-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:16Zoai:ri.conicet.gov.ar:11336/34270instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:16.843CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans
title Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans
spellingShingle Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans
Franco, Diana Lorena
Circadian Rhythms
Fungi
Bioinformatics
Oscillatory systems
title_short Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans
title_full Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans
title_fullStr Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans
title_full_unstemmed Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans
title_sort Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans
dc.creator.none.fl_str_mv Franco, Diana Lorena
Canessa, Paulo
Bellora, Nicolás
Risau Gusman, Sebastian Luis
Olivares Yañez, Consuelo
Pérez Lara, Rodrigo
Libkind Frati, Diego
Larrondo, Luis F.
Marpegan, Luciano
author Franco, Diana Lorena
author_facet Franco, Diana Lorena
Canessa, Paulo
Bellora, Nicolás
Risau Gusman, Sebastian Luis
Olivares Yañez, Consuelo
Pérez Lara, Rodrigo
Libkind Frati, Diego
Larrondo, Luis F.
Marpegan, Luciano
author_role author
author2 Canessa, Paulo
Bellora, Nicolás
Risau Gusman, Sebastian Luis
Olivares Yañez, Consuelo
Pérez Lara, Rodrigo
Libkind Frati, Diego
Larrondo, Luis F.
Marpegan, Luciano
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Circadian Rhythms
Fungi
Bioinformatics
Oscillatory systems
topic Circadian Rhythms
Fungi
Bioinformatics
Oscillatory systems
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Circadian systems enable organisms to synchronize their physiology to daily and seasonal environmental changes relying on endogenous pacemakers that oscillate with a period close to 24 h even in the absence of external timing cues. The oscillations are achieved by intracellular transcriptional/translational feedback loops thoroughly characterized for many organisms, but still little is known about the presence and characteristics of circadian clocks in fungi other than Neurospora crassa. We sought to characterize the circadian system of a natural isolate of Aureobasidium pullulans, a cold-adapted yeast bearing great biotechnological potential. A. pullulans formed daily concentric rings that were synchronized by light/dark cycles and were also formed in constant darkness with a period of 24.5 h. Moreover, these rhythms were temperature compensated, as evidenced by experiments conducted at temperatures as low as 10 °C. Finally, the expression of clock-essential genes, frequency, white collar-1, white collar-2 and vivid was confirmed. In summary, our results indicate the existence of a functional circadian clock in A. pullulans, capable of sustaining rhythms at very low temperatures and, based on the presence of conserved clock-gene homologues, suggest a molecular and functional relationship to well-described circadian systems.
Fil: Franco, Diana Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Canessa, Paulo. Universidad Andrés Bello; Chile. Millennium Nucleus for Fungal Integrative and Synthetic Biology; Chile
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Risau Gusman, Sebastian Luis. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olivares Yañez, Consuelo. Pontificia Universidad Católica de Chile; Chile. Millennium Nucleus for Fungal Integrative and Synthetic Biology; Chile
Fil: Pérez Lara, Rodrigo. Pontificia Universidad Católica de Chile; Chile. Millennium Nucleus for Fungal Integrative and Synthetic Biology; Chile
Fil: Libkind Frati, Diego. Pontificia Universidad Católica de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Larrondo, Luis F.. Millennium Nucleus for Fungal Integrative and Synthetic Biology; Chile
Fil: Marpegan, Luciano. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Circadian systems enable organisms to synchronize their physiology to daily and seasonal environmental changes relying on endogenous pacemakers that oscillate with a period close to 24 h even in the absence of external timing cues. The oscillations are achieved by intracellular transcriptional/translational feedback loops thoroughly characterized for many organisms, but still little is known about the presence and characteristics of circadian clocks in fungi other than Neurospora crassa. We sought to characterize the circadian system of a natural isolate of Aureobasidium pullulans, a cold-adapted yeast bearing great biotechnological potential. A. pullulans formed daily concentric rings that were synchronized by light/dark cycles and were also formed in constant darkness with a period of 24.5 h. Moreover, these rhythms were temperature compensated, as evidenced by experiments conducted at temperatures as low as 10 °C. Finally, the expression of clock-essential genes, frequency, white collar-1, white collar-2 and vivid was confirmed. In summary, our results indicate the existence of a functional circadian clock in A. pullulans, capable of sustaining rhythms at very low temperatures and, based on the presence of conserved clock-gene homologues, suggest a molecular and functional relationship to well-described circadian systems.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34270
Franco, Diana Lorena; Canessa, Paulo; Bellora, Nicolás; Risau Gusman, Sebastian Luis; Olivares Yañez, Consuelo; et al.; Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans; Nature Publishing Group; Scientific Reports; 7; 1; 10-2017; 1-12; 13837
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34270
identifier_str_mv Franco, Diana Lorena; Canessa, Paulo; Bellora, Nicolás; Risau Gusman, Sebastian Luis; Olivares Yañez, Consuelo; et al.; Spontaneous circadian rhythms in a cold-adapted natural isolate of Aureobasidium pullulans; Nature Publishing Group; Scientific Reports; 7; 1; 10-2017; 1-12; 13837
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-017-14085-6
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-14085-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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