Effect of an acute glucose overload on islet cell morphology and secretory function in the toad
- Autores
- Francini, Flavio; del Zotto, Hector Herminio; Gagliardino, Juan Jose
- Año de publicación
- 2001
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of this work was to study the effect of induced hyperglycemia on islet cell mass and insulin secretion in normal toads. Immunolabeled β cell area, replication (bromodeoxyuridine) and apoptosis (propidium iodide) rate, islet neogenesis (cytokeratin), and insulin secretion in vitro were measured in adult male specimens of Bufo arenarum during and after interruption of the injection of either a 50% glucose solution (2 g/100 g) or its vehicle for 4 days. Glucose administration caused hyperglycemia (122.6 ± 16.7 and 508.3 ± 115.9 mg/dl vs 23.5 ± 1.26 and 22.8 ± 1.8 mg/dl, at days 3 and 5, respectively, P < 0.05) and a significant decrease in the number of islets/mm2 (day 3: 9.7 ± 0.9 vs 3.3 ± 0.4, P < 0.05; day 5: 9.4 ± 0.8 vs 7.4 ± 0.6; day 9: 9.6 ± 0.9 vs 6.2 ± 0.4, P < 0.05) and in the percentage of immunolabeled β cell area (day 3: 2.07 ± 0.2 vs 0.5 ± 0.1%, P < 0.05; day 5: 1.8 ± 0.1 vs 0.6 ± 0.1%; day 9: 1.7 ± 0.1 vs 0.7 ± 0.1%, P < 0.05). Glucose-injected animals had a simultaneous significantly higher percentage of BrdU-labeled β cells (day 3: 0.46 ± 0.02 vs 0.23 ± 0.03%; day 5: 0.54 ± 0.13 vs 0.22 ± 0.02%; day 9: 0.61 ± 0.0 vs 0.27 ± 0.05%, P < 0.05) and cytokeratin-labeled endocrine cells (day 3: 0.21 ± 0.06 vs 0.01 ± 0.00%; day 5: 0.17 ± 0.06 vs 0.01 ± 0.01%; day 9: 1.25 ± 0.2 vs 0.01 ± 0.008%, P < 0.05) and a higher rate of apoptotic β cells (day 3: 0.14 ± 0.04 vs 0.05 ± 0.02%; day 5: 0.4 ± 0.06 vs 0.05 ± 0.2, P < 0.05; day 9: 0.47 ± 0.04 vs 0.06 ± 0.03, P < 0.05). Comparable amounts of insulin were secreted in vitro by both groups in response to 2 mM glucose, whereas there was a significantly reduced response to 8 mM glucose in treated animals (day 3: 73 ± 12 vs 165 ± 20%; day 5: 74 ± 11 vs 204 ± 18%, P < 0.05). This decreased response to high glucose reverted to normal after removal of the glucose injection. These results show for the first time that short-term hyperglycemia triggers marked morphological and transient secretory changes in the toad pancreas similar in part to those elicited in the pancreas of several mammals. As with other results previously reported, these results support the usefulness of the toad as an alternative easily handled model to study the growth and secretory function of the endocrine pancreas.
Fil: Francini, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: del Zotto, Hector Herminio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina - Materia
-
APOPTOSIS
BUFO ARENARUM
CYTOKERATIN
HYPERGLYCEMIA
INSULIN SECRETION
ISLET FUNCTION
ISLET NEOGENESIS
Β CELL REPLICATION RATE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/142374
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Effect of an acute glucose overload on islet cell morphology and secretory function in the toadFrancini, Flaviodel Zotto, Hector HerminioGagliardino, Juan JoseAPOPTOSISBUFO ARENARUMCYTOKERATINHYPERGLYCEMIAINSULIN SECRETIONISLET FUNCTIONISLET NEOGENESISΒ CELL REPLICATION RATEhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The aim of this work was to study the effect of induced hyperglycemia on islet cell mass and insulin secretion in normal toads. Immunolabeled β cell area, replication (bromodeoxyuridine) and apoptosis (propidium iodide) rate, islet neogenesis (cytokeratin), and insulin secretion in vitro were measured in adult male specimens of Bufo arenarum during and after interruption of the injection of either a 50% glucose solution (2 g/100 g) or its vehicle for 4 days. Glucose administration caused hyperglycemia (122.6 ± 16.7 and 508.3 ± 115.9 mg/dl vs 23.5 ± 1.26 and 22.8 ± 1.8 mg/dl, at days 3 and 5, respectively, P < 0.05) and a significant decrease in the number of islets/mm2 (day 3: 9.7 ± 0.9 vs 3.3 ± 0.4, P < 0.05; day 5: 9.4 ± 0.8 vs 7.4 ± 0.6; day 9: 9.6 ± 0.9 vs 6.2 ± 0.4, P < 0.05) and in the percentage of immunolabeled β cell area (day 3: 2.07 ± 0.2 vs 0.5 ± 0.1%, P < 0.05; day 5: 1.8 ± 0.1 vs 0.6 ± 0.1%; day 9: 1.7 ± 0.1 vs 0.7 ± 0.1%, P < 0.05). Glucose-injected animals had a simultaneous significantly higher percentage of BrdU-labeled β cells (day 3: 0.46 ± 0.02 vs 0.23 ± 0.03%; day 5: 0.54 ± 0.13 vs 0.22 ± 0.02%; day 9: 0.61 ± 0.0 vs 0.27 ± 0.05%, P < 0.05) and cytokeratin-labeled endocrine cells (day 3: 0.21 ± 0.06 vs 0.01 ± 0.00%; day 5: 0.17 ± 0.06 vs 0.01 ± 0.01%; day 9: 1.25 ± 0.2 vs 0.01 ± 0.008%, P < 0.05) and a higher rate of apoptotic β cells (day 3: 0.14 ± 0.04 vs 0.05 ± 0.02%; day 5: 0.4 ± 0.06 vs 0.05 ± 0.2, P < 0.05; day 9: 0.47 ± 0.04 vs 0.06 ± 0.03, P < 0.05). Comparable amounts of insulin were secreted in vitro by both groups in response to 2 mM glucose, whereas there was a significantly reduced response to 8 mM glucose in treated animals (day 3: 73 ± 12 vs 165 ± 20%; day 5: 74 ± 11 vs 204 ± 18%, P < 0.05). This decreased response to high glucose reverted to normal after removal of the glucose injection. These results show for the first time that short-term hyperglycemia triggers marked morphological and transient secretory changes in the toad pancreas similar in part to those elicited in the pancreas of several mammals. As with other results previously reported, these results support the usefulness of the toad as an alternative easily handled model to study the growth and secretory function of the endocrine pancreas.Fil: Francini, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaFil: del Zotto, Hector Herminio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaFil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaAcademic Press Inc Elsevier Science2001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142374Francini, Flavio; del Zotto, Hector Herminio; Gagliardino, Juan Jose; Effect of an acute glucose overload on islet cell morphology and secretory function in the toad; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 122; 2; 12-2001; 130-1380016-6480CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0016648001976178?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1006/gcen.2001.7617info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:41Zoai:ri.conicet.gov.ar:11336/142374instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:41.824CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Effect of an acute glucose overload on islet cell morphology and secretory function in the toad |
title |
Effect of an acute glucose overload on islet cell morphology and secretory function in the toad |
spellingShingle |
Effect of an acute glucose overload on islet cell morphology and secretory function in the toad Francini, Flavio APOPTOSIS BUFO ARENARUM CYTOKERATIN HYPERGLYCEMIA INSULIN SECRETION ISLET FUNCTION ISLET NEOGENESIS Β CELL REPLICATION RATE |
title_short |
Effect of an acute glucose overload on islet cell morphology and secretory function in the toad |
title_full |
Effect of an acute glucose overload on islet cell morphology and secretory function in the toad |
title_fullStr |
Effect of an acute glucose overload on islet cell morphology and secretory function in the toad |
title_full_unstemmed |
Effect of an acute glucose overload on islet cell morphology and secretory function in the toad |
title_sort |
Effect of an acute glucose overload on islet cell morphology and secretory function in the toad |
dc.creator.none.fl_str_mv |
Francini, Flavio del Zotto, Hector Herminio Gagliardino, Juan Jose |
author |
Francini, Flavio |
author_facet |
Francini, Flavio del Zotto, Hector Herminio Gagliardino, Juan Jose |
author_role |
author |
author2 |
del Zotto, Hector Herminio Gagliardino, Juan Jose |
author2_role |
author author |
dc.subject.none.fl_str_mv |
APOPTOSIS BUFO ARENARUM CYTOKERATIN HYPERGLYCEMIA INSULIN SECRETION ISLET FUNCTION ISLET NEOGENESIS Β CELL REPLICATION RATE |
topic |
APOPTOSIS BUFO ARENARUM CYTOKERATIN HYPERGLYCEMIA INSULIN SECRETION ISLET FUNCTION ISLET NEOGENESIS Β CELL REPLICATION RATE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The aim of this work was to study the effect of induced hyperglycemia on islet cell mass and insulin secretion in normal toads. Immunolabeled β cell area, replication (bromodeoxyuridine) and apoptosis (propidium iodide) rate, islet neogenesis (cytokeratin), and insulin secretion in vitro were measured in adult male specimens of Bufo arenarum during and after interruption of the injection of either a 50% glucose solution (2 g/100 g) or its vehicle for 4 days. Glucose administration caused hyperglycemia (122.6 ± 16.7 and 508.3 ± 115.9 mg/dl vs 23.5 ± 1.26 and 22.8 ± 1.8 mg/dl, at days 3 and 5, respectively, P < 0.05) and a significant decrease in the number of islets/mm2 (day 3: 9.7 ± 0.9 vs 3.3 ± 0.4, P < 0.05; day 5: 9.4 ± 0.8 vs 7.4 ± 0.6; day 9: 9.6 ± 0.9 vs 6.2 ± 0.4, P < 0.05) and in the percentage of immunolabeled β cell area (day 3: 2.07 ± 0.2 vs 0.5 ± 0.1%, P < 0.05; day 5: 1.8 ± 0.1 vs 0.6 ± 0.1%; day 9: 1.7 ± 0.1 vs 0.7 ± 0.1%, P < 0.05). Glucose-injected animals had a simultaneous significantly higher percentage of BrdU-labeled β cells (day 3: 0.46 ± 0.02 vs 0.23 ± 0.03%; day 5: 0.54 ± 0.13 vs 0.22 ± 0.02%; day 9: 0.61 ± 0.0 vs 0.27 ± 0.05%, P < 0.05) and cytokeratin-labeled endocrine cells (day 3: 0.21 ± 0.06 vs 0.01 ± 0.00%; day 5: 0.17 ± 0.06 vs 0.01 ± 0.01%; day 9: 1.25 ± 0.2 vs 0.01 ± 0.008%, P < 0.05) and a higher rate of apoptotic β cells (day 3: 0.14 ± 0.04 vs 0.05 ± 0.02%; day 5: 0.4 ± 0.06 vs 0.05 ± 0.2, P < 0.05; day 9: 0.47 ± 0.04 vs 0.06 ± 0.03, P < 0.05). Comparable amounts of insulin were secreted in vitro by both groups in response to 2 mM glucose, whereas there was a significantly reduced response to 8 mM glucose in treated animals (day 3: 73 ± 12 vs 165 ± 20%; day 5: 74 ± 11 vs 204 ± 18%, P < 0.05). This decreased response to high glucose reverted to normal after removal of the glucose injection. These results show for the first time that short-term hyperglycemia triggers marked morphological and transient secretory changes in the toad pancreas similar in part to those elicited in the pancreas of several mammals. As with other results previously reported, these results support the usefulness of the toad as an alternative easily handled model to study the growth and secretory function of the endocrine pancreas. Fil: Francini, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina Fil: del Zotto, Hector Herminio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina |
description |
The aim of this work was to study the effect of induced hyperglycemia on islet cell mass and insulin secretion in normal toads. Immunolabeled β cell area, replication (bromodeoxyuridine) and apoptosis (propidium iodide) rate, islet neogenesis (cytokeratin), and insulin secretion in vitro were measured in adult male specimens of Bufo arenarum during and after interruption of the injection of either a 50% glucose solution (2 g/100 g) or its vehicle for 4 days. Glucose administration caused hyperglycemia (122.6 ± 16.7 and 508.3 ± 115.9 mg/dl vs 23.5 ± 1.26 and 22.8 ± 1.8 mg/dl, at days 3 and 5, respectively, P < 0.05) and a significant decrease in the number of islets/mm2 (day 3: 9.7 ± 0.9 vs 3.3 ± 0.4, P < 0.05; day 5: 9.4 ± 0.8 vs 7.4 ± 0.6; day 9: 9.6 ± 0.9 vs 6.2 ± 0.4, P < 0.05) and in the percentage of immunolabeled β cell area (day 3: 2.07 ± 0.2 vs 0.5 ± 0.1%, P < 0.05; day 5: 1.8 ± 0.1 vs 0.6 ± 0.1%; day 9: 1.7 ± 0.1 vs 0.7 ± 0.1%, P < 0.05). Glucose-injected animals had a simultaneous significantly higher percentage of BrdU-labeled β cells (day 3: 0.46 ± 0.02 vs 0.23 ± 0.03%; day 5: 0.54 ± 0.13 vs 0.22 ± 0.02%; day 9: 0.61 ± 0.0 vs 0.27 ± 0.05%, P < 0.05) and cytokeratin-labeled endocrine cells (day 3: 0.21 ± 0.06 vs 0.01 ± 0.00%; day 5: 0.17 ± 0.06 vs 0.01 ± 0.01%; day 9: 1.25 ± 0.2 vs 0.01 ± 0.008%, P < 0.05) and a higher rate of apoptotic β cells (day 3: 0.14 ± 0.04 vs 0.05 ± 0.02%; day 5: 0.4 ± 0.06 vs 0.05 ± 0.2, P < 0.05; day 9: 0.47 ± 0.04 vs 0.06 ± 0.03, P < 0.05). Comparable amounts of insulin were secreted in vitro by both groups in response to 2 mM glucose, whereas there was a significantly reduced response to 8 mM glucose in treated animals (day 3: 73 ± 12 vs 165 ± 20%; day 5: 74 ± 11 vs 204 ± 18%, P < 0.05). This decreased response to high glucose reverted to normal after removal of the glucose injection. These results show for the first time that short-term hyperglycemia triggers marked morphological and transient secretory changes in the toad pancreas similar in part to those elicited in the pancreas of several mammals. As with other results previously reported, these results support the usefulness of the toad as an alternative easily handled model to study the growth and secretory function of the endocrine pancreas. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/142374 Francini, Flavio; del Zotto, Hector Herminio; Gagliardino, Juan Jose; Effect of an acute glucose overload on islet cell morphology and secretory function in the toad; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 122; 2; 12-2001; 130-138 0016-6480 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/142374 |
identifier_str_mv |
Francini, Flavio; del Zotto, Hector Herminio; Gagliardino, Juan Jose; Effect of an acute glucose overload on islet cell morphology and secretory function in the toad; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 122; 2; 12-2001; 130-138 0016-6480 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0016648001976178?via%3Dihub info:eu-repo/semantics/altIdentifier/doi/10.1006/gcen.2001.7617 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614375814463488 |
score |
13.070432 |