Effect of an acute glucose overload on islet cell morphology and secretory function in the toad

Autores
Francini, Flavio; del Zotto, Hector Herminio; Gagliardino, Juan Jose
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to study the effect of induced hyperglycemia on islet cell mass and insulin secretion in normal toads. Immunolabeled β cell area, replication (bromodeoxyuridine) and apoptosis (propidium iodide) rate, islet neogenesis (cytokeratin), and insulin secretion in vitro were measured in adult male specimens of Bufo arenarum during and after interruption of the injection of either a 50% glucose solution (2 g/100 g) or its vehicle for 4 days. Glucose administration caused hyperglycemia (122.6 ± 16.7 and 508.3 ± 115.9 mg/dl vs 23.5 ± 1.26 and 22.8 ± 1.8 mg/dl, at days 3 and 5, respectively, P < 0.05) and a significant decrease in the number of islets/mm2 (day 3: 9.7 ± 0.9 vs 3.3 ± 0.4, P < 0.05; day 5: 9.4 ± 0.8 vs 7.4 ± 0.6; day 9: 9.6 ± 0.9 vs 6.2 ± 0.4, P < 0.05) and in the percentage of immunolabeled β cell area (day 3: 2.07 ± 0.2 vs 0.5 ± 0.1%, P < 0.05; day 5: 1.8 ± 0.1 vs 0.6 ± 0.1%; day 9: 1.7 ± 0.1 vs 0.7 ± 0.1%, P < 0.05). Glucose-injected animals had a simultaneous significantly higher percentage of BrdU-labeled β cells (day 3: 0.46 ± 0.02 vs 0.23 ± 0.03%; day 5: 0.54 ± 0.13 vs 0.22 ± 0.02%; day 9: 0.61 ± 0.0 vs 0.27 ± 0.05%, P < 0.05) and cytokeratin-labeled endocrine cells (day 3: 0.21 ± 0.06 vs 0.01 ± 0.00%; day 5: 0.17 ± 0.06 vs 0.01 ± 0.01%; day 9: 1.25 ± 0.2 vs 0.01 ± 0.008%, P < 0.05) and a higher rate of apoptotic β cells (day 3: 0.14 ± 0.04 vs 0.05 ± 0.02%; day 5: 0.4 ± 0.06 vs 0.05 ± 0.2, P < 0.05; day 9: 0.47 ± 0.04 vs 0.06 ± 0.03, P < 0.05). Comparable amounts of insulin were secreted in vitro by both groups in response to 2 mM glucose, whereas there was a significantly reduced response to 8 mM glucose in treated animals (day 3: 73 ± 12 vs 165 ± 20%; day 5: 74 ± 11 vs 204 ± 18%, P < 0.05). This decreased response to high glucose reverted to normal after removal of the glucose injection. These results show for the first time that short-term hyperglycemia triggers marked morphological and transient secretory changes in the toad pancreas similar in part to those elicited in the pancreas of several mammals. As with other results previously reported, these results support the usefulness of the toad as an alternative easily handled model to study the growth and secretory function of the endocrine pancreas.
Fil: Francini, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: del Zotto, Hector Herminio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Materia
APOPTOSIS
BUFO ARENARUM
CYTOKERATIN
HYPERGLYCEMIA
INSULIN SECRETION
ISLET FUNCTION
ISLET NEOGENESIS
Β CELL REPLICATION RATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142374

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oai_identifier_str oai:ri.conicet.gov.ar:11336/142374
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of an acute glucose overload on islet cell morphology and secretory function in the toadFrancini, Flaviodel Zotto, Hector HerminioGagliardino, Juan JoseAPOPTOSISBUFO ARENARUMCYTOKERATINHYPERGLYCEMIAINSULIN SECRETIONISLET FUNCTIONISLET NEOGENESISΒ CELL REPLICATION RATEhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The aim of this work was to study the effect of induced hyperglycemia on islet cell mass and insulin secretion in normal toads. Immunolabeled β cell area, replication (bromodeoxyuridine) and apoptosis (propidium iodide) rate, islet neogenesis (cytokeratin), and insulin secretion in vitro were measured in adult male specimens of Bufo arenarum during and after interruption of the injection of either a 50% glucose solution (2 g/100 g) or its vehicle for 4 days. Glucose administration caused hyperglycemia (122.6 ± 16.7 and 508.3 ± 115.9 mg/dl vs 23.5 ± 1.26 and 22.8 ± 1.8 mg/dl, at days 3 and 5, respectively, P < 0.05) and a significant decrease in the number of islets/mm2 (day 3: 9.7 ± 0.9 vs 3.3 ± 0.4, P < 0.05; day 5: 9.4 ± 0.8 vs 7.4 ± 0.6; day 9: 9.6 ± 0.9 vs 6.2 ± 0.4, P < 0.05) and in the percentage of immunolabeled β cell area (day 3: 2.07 ± 0.2 vs 0.5 ± 0.1%, P < 0.05; day 5: 1.8 ± 0.1 vs 0.6 ± 0.1%; day 9: 1.7 ± 0.1 vs 0.7 ± 0.1%, P < 0.05). Glucose-injected animals had a simultaneous significantly higher percentage of BrdU-labeled β cells (day 3: 0.46 ± 0.02 vs 0.23 ± 0.03%; day 5: 0.54 ± 0.13 vs 0.22 ± 0.02%; day 9: 0.61 ± 0.0 vs 0.27 ± 0.05%, P < 0.05) and cytokeratin-labeled endocrine cells (day 3: 0.21 ± 0.06 vs 0.01 ± 0.00%; day 5: 0.17 ± 0.06 vs 0.01 ± 0.01%; day 9: 1.25 ± 0.2 vs 0.01 ± 0.008%, P < 0.05) and a higher rate of apoptotic β cells (day 3: 0.14 ± 0.04 vs 0.05 ± 0.02%; day 5: 0.4 ± 0.06 vs 0.05 ± 0.2, P < 0.05; day 9: 0.47 ± 0.04 vs 0.06 ± 0.03, P < 0.05). Comparable amounts of insulin were secreted in vitro by both groups in response to 2 mM glucose, whereas there was a significantly reduced response to 8 mM glucose in treated animals (day 3: 73 ± 12 vs 165 ± 20%; day 5: 74 ± 11 vs 204 ± 18%, P < 0.05). This decreased response to high glucose reverted to normal after removal of the glucose injection. These results show for the first time that short-term hyperglycemia triggers marked morphological and transient secretory changes in the toad pancreas similar in part to those elicited in the pancreas of several mammals. As with other results previously reported, these results support the usefulness of the toad as an alternative easily handled model to study the growth and secretory function of the endocrine pancreas.Fil: Francini, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaFil: del Zotto, Hector Herminio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaFil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaAcademic Press Inc Elsevier Science2001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142374Francini, Flavio; del Zotto, Hector Herminio; Gagliardino, Juan Jose; Effect of an acute glucose overload on islet cell morphology and secretory function in the toad; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 122; 2; 12-2001; 130-1380016-6480CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0016648001976178?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1006/gcen.2001.7617info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:41Zoai:ri.conicet.gov.ar:11336/142374instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:41.824CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of an acute glucose overload on islet cell morphology and secretory function in the toad
title Effect of an acute glucose overload on islet cell morphology and secretory function in the toad
spellingShingle Effect of an acute glucose overload on islet cell morphology and secretory function in the toad
Francini, Flavio
APOPTOSIS
BUFO ARENARUM
CYTOKERATIN
HYPERGLYCEMIA
INSULIN SECRETION
ISLET FUNCTION
ISLET NEOGENESIS
Β CELL REPLICATION RATE
title_short Effect of an acute glucose overload on islet cell morphology and secretory function in the toad
title_full Effect of an acute glucose overload on islet cell morphology and secretory function in the toad
title_fullStr Effect of an acute glucose overload on islet cell morphology and secretory function in the toad
title_full_unstemmed Effect of an acute glucose overload on islet cell morphology and secretory function in the toad
title_sort Effect of an acute glucose overload on islet cell morphology and secretory function in the toad
dc.creator.none.fl_str_mv Francini, Flavio
del Zotto, Hector Herminio
Gagliardino, Juan Jose
author Francini, Flavio
author_facet Francini, Flavio
del Zotto, Hector Herminio
Gagliardino, Juan Jose
author_role author
author2 del Zotto, Hector Herminio
Gagliardino, Juan Jose
author2_role author
author
dc.subject.none.fl_str_mv APOPTOSIS
BUFO ARENARUM
CYTOKERATIN
HYPERGLYCEMIA
INSULIN SECRETION
ISLET FUNCTION
ISLET NEOGENESIS
Β CELL REPLICATION RATE
topic APOPTOSIS
BUFO ARENARUM
CYTOKERATIN
HYPERGLYCEMIA
INSULIN SECRETION
ISLET FUNCTION
ISLET NEOGENESIS
Β CELL REPLICATION RATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this work was to study the effect of induced hyperglycemia on islet cell mass and insulin secretion in normal toads. Immunolabeled β cell area, replication (bromodeoxyuridine) and apoptosis (propidium iodide) rate, islet neogenesis (cytokeratin), and insulin secretion in vitro were measured in adult male specimens of Bufo arenarum during and after interruption of the injection of either a 50% glucose solution (2 g/100 g) or its vehicle for 4 days. Glucose administration caused hyperglycemia (122.6 ± 16.7 and 508.3 ± 115.9 mg/dl vs 23.5 ± 1.26 and 22.8 ± 1.8 mg/dl, at days 3 and 5, respectively, P < 0.05) and a significant decrease in the number of islets/mm2 (day 3: 9.7 ± 0.9 vs 3.3 ± 0.4, P < 0.05; day 5: 9.4 ± 0.8 vs 7.4 ± 0.6; day 9: 9.6 ± 0.9 vs 6.2 ± 0.4, P < 0.05) and in the percentage of immunolabeled β cell area (day 3: 2.07 ± 0.2 vs 0.5 ± 0.1%, P < 0.05; day 5: 1.8 ± 0.1 vs 0.6 ± 0.1%; day 9: 1.7 ± 0.1 vs 0.7 ± 0.1%, P < 0.05). Glucose-injected animals had a simultaneous significantly higher percentage of BrdU-labeled β cells (day 3: 0.46 ± 0.02 vs 0.23 ± 0.03%; day 5: 0.54 ± 0.13 vs 0.22 ± 0.02%; day 9: 0.61 ± 0.0 vs 0.27 ± 0.05%, P < 0.05) and cytokeratin-labeled endocrine cells (day 3: 0.21 ± 0.06 vs 0.01 ± 0.00%; day 5: 0.17 ± 0.06 vs 0.01 ± 0.01%; day 9: 1.25 ± 0.2 vs 0.01 ± 0.008%, P < 0.05) and a higher rate of apoptotic β cells (day 3: 0.14 ± 0.04 vs 0.05 ± 0.02%; day 5: 0.4 ± 0.06 vs 0.05 ± 0.2, P < 0.05; day 9: 0.47 ± 0.04 vs 0.06 ± 0.03, P < 0.05). Comparable amounts of insulin were secreted in vitro by both groups in response to 2 mM glucose, whereas there was a significantly reduced response to 8 mM glucose in treated animals (day 3: 73 ± 12 vs 165 ± 20%; day 5: 74 ± 11 vs 204 ± 18%, P < 0.05). This decreased response to high glucose reverted to normal after removal of the glucose injection. These results show for the first time that short-term hyperglycemia triggers marked morphological and transient secretory changes in the toad pancreas similar in part to those elicited in the pancreas of several mammals. As with other results previously reported, these results support the usefulness of the toad as an alternative easily handled model to study the growth and secretory function of the endocrine pancreas.
Fil: Francini, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: del Zotto, Hector Herminio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
description The aim of this work was to study the effect of induced hyperglycemia on islet cell mass and insulin secretion in normal toads. Immunolabeled β cell area, replication (bromodeoxyuridine) and apoptosis (propidium iodide) rate, islet neogenesis (cytokeratin), and insulin secretion in vitro were measured in adult male specimens of Bufo arenarum during and after interruption of the injection of either a 50% glucose solution (2 g/100 g) or its vehicle for 4 days. Glucose administration caused hyperglycemia (122.6 ± 16.7 and 508.3 ± 115.9 mg/dl vs 23.5 ± 1.26 and 22.8 ± 1.8 mg/dl, at days 3 and 5, respectively, P < 0.05) and a significant decrease in the number of islets/mm2 (day 3: 9.7 ± 0.9 vs 3.3 ± 0.4, P < 0.05; day 5: 9.4 ± 0.8 vs 7.4 ± 0.6; day 9: 9.6 ± 0.9 vs 6.2 ± 0.4, P < 0.05) and in the percentage of immunolabeled β cell area (day 3: 2.07 ± 0.2 vs 0.5 ± 0.1%, P < 0.05; day 5: 1.8 ± 0.1 vs 0.6 ± 0.1%; day 9: 1.7 ± 0.1 vs 0.7 ± 0.1%, P < 0.05). Glucose-injected animals had a simultaneous significantly higher percentage of BrdU-labeled β cells (day 3: 0.46 ± 0.02 vs 0.23 ± 0.03%; day 5: 0.54 ± 0.13 vs 0.22 ± 0.02%; day 9: 0.61 ± 0.0 vs 0.27 ± 0.05%, P < 0.05) and cytokeratin-labeled endocrine cells (day 3: 0.21 ± 0.06 vs 0.01 ± 0.00%; day 5: 0.17 ± 0.06 vs 0.01 ± 0.01%; day 9: 1.25 ± 0.2 vs 0.01 ± 0.008%, P < 0.05) and a higher rate of apoptotic β cells (day 3: 0.14 ± 0.04 vs 0.05 ± 0.02%; day 5: 0.4 ± 0.06 vs 0.05 ± 0.2, P < 0.05; day 9: 0.47 ± 0.04 vs 0.06 ± 0.03, P < 0.05). Comparable amounts of insulin were secreted in vitro by both groups in response to 2 mM glucose, whereas there was a significantly reduced response to 8 mM glucose in treated animals (day 3: 73 ± 12 vs 165 ± 20%; day 5: 74 ± 11 vs 204 ± 18%, P < 0.05). This decreased response to high glucose reverted to normal after removal of the glucose injection. These results show for the first time that short-term hyperglycemia triggers marked morphological and transient secretory changes in the toad pancreas similar in part to those elicited in the pancreas of several mammals. As with other results previously reported, these results support the usefulness of the toad as an alternative easily handled model to study the growth and secretory function of the endocrine pancreas.
publishDate 2001
dc.date.none.fl_str_mv 2001-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142374
Francini, Flavio; del Zotto, Hector Herminio; Gagliardino, Juan Jose; Effect of an acute glucose overload on islet cell morphology and secretory function in the toad; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 122; 2; 12-2001; 130-138
0016-6480
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142374
identifier_str_mv Francini, Flavio; del Zotto, Hector Herminio; Gagliardino, Juan Jose; Effect of an acute glucose overload on islet cell morphology and secretory function in the toad; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 122; 2; 12-2001; 130-138
0016-6480
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0016648001976178?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1006/gcen.2001.7617
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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