Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans

Autores
Serradell, Marianela del Carmen.; Saura, Alicia; Rupil, Lucia; Gargantini, Pablo Ruben; Faya, Marcela Inés; Furlan, Paulina; Lujan, Hugo Daniel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Giardia lamblia is a human intestinal parasite and one of the most frequent enteric pathogen of companion animals. Clinical manifestations of giardiasis, such as diarrhoea, anorexia, weight loss and lethargy, have been associated with Giardia infections in both domestic and farm animals. A few anti-parasitic drugs are routinely used to treat giardiasis, but re-infections are common and drug-resistant strains have already been reported. Unfortunately, efficient vaccines against Giardia are not available. Giardia undergoes antigenic variation; through this mechanism, parasites can avoid the host?s immune defenses, causing chronic infections and/or re-infections. Antigenic variation is characterised by a continuous switch in the expression of members of a homologous family of genes encoding surface antigens. In a previous report, we indicated that in Giardia, the mechanism responsible for the exchange of variant-specific surface proteins (VSPs) involves the RNA interference (RNAi) pathway. From a repertoire of ~200 VSP genes, only one is expressed on the surface of single trophozoites; however, RNAi machinery disruption generates trophozoites that express the complete VSP repertoire. We also demonstrated that gerbils orally immunised with VSPs isolated from these altered parasites showed high levels of protection. Here we tested this vaccine in cats and dogs, and found that it is highly efficient in preventing new infections and reducing chronic giardiasis in domestic animals both in experimental and natural infections. Remarkably, immunisation of dogs in a highly endemic area strongly decreased the percentage of infected children in the community, suggesting that this vaccine would block the zoonotic transmission of the disease.
Fil: Serradell, Marianela del Carmen.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; Argentina
Fil: Saura, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; Argentina
Fil: Rupil, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; Argentina
Fil: Gargantini, Pablo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; Argentina
Fil: Faya, Marcela Inés. Universidad Católica de Córdoba; Argentina
Fil: Furlan, Paulina. Universidad Católica de Córdoba; Argentina
Fil: Lujan, Hugo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; Argentina
Materia
PARASITIC INFECTION
PROTEIN VACCINES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40645
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humansSerradell, Marianela del Carmen.Saura, AliciaRupil, LuciaGargantini, Pablo RubenFaya, Marcela InésFurlan, PaulinaLujan, Hugo DanielPARASITIC INFECTIONPROTEIN VACCINEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Giardia lamblia is a human intestinal parasite and one of the most frequent enteric pathogen of companion animals. Clinical manifestations of giardiasis, such as diarrhoea, anorexia, weight loss and lethargy, have been associated with Giardia infections in both domestic and farm animals. A few anti-parasitic drugs are routinely used to treat giardiasis, but re-infections are common and drug-resistant strains have already been reported. Unfortunately, efficient vaccines against Giardia are not available. Giardia undergoes antigenic variation; through this mechanism, parasites can avoid the host?s immune defenses, causing chronic infections and/or re-infections. Antigenic variation is characterised by a continuous switch in the expression of members of a homologous family of genes encoding surface antigens. In a previous report, we indicated that in Giardia, the mechanism responsible for the exchange of variant-specific surface proteins (VSPs) involves the RNA interference (RNAi) pathway. From a repertoire of ~200 VSP genes, only one is expressed on the surface of single trophozoites; however, RNAi machinery disruption generates trophozoites that express the complete VSP repertoire. We also demonstrated that gerbils orally immunised with VSPs isolated from these altered parasites showed high levels of protection. Here we tested this vaccine in cats and dogs, and found that it is highly efficient in preventing new infections and reducing chronic giardiasis in domestic animals both in experimental and natural infections. Remarkably, immunisation of dogs in a highly endemic area strongly decreased the percentage of infected children in the community, suggesting that this vaccine would block the zoonotic transmission of the disease.Fil: Serradell, Marianela del Carmen.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; ArgentinaFil: Saura, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; ArgentinaFil: Rupil, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; ArgentinaFil: Gargantini, Pablo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; ArgentinaFil: Faya, Marcela Inés. Universidad Católica de Córdoba; ArgentinaFil: Furlan, Paulina. Universidad Católica de Córdoba; ArgentinaFil: Lujan, Hugo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; ArgentinaSpringer Nature2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40645Serradell, Marianela del Carmen.; Saura, Alicia; Rupil, Lucia; Gargantini, Pablo Ruben; Faya, Marcela Inés; et al.; Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans; Springer Nature; npj vaccines; 9-2016; 1-112059-0105CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/npjvaccines201618info:eu-repo/semantics/altIdentifier/doi/10.1038/npjvaccines.2016.18info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:12Zoai:ri.conicet.gov.ar:11336/40645instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:12.396CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans
title Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans
spellingShingle Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans
Serradell, Marianela del Carmen.
PARASITIC INFECTION
PROTEIN VACCINES
title_short Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans
title_full Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans
title_fullStr Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans
title_full_unstemmed Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans
title_sort Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans
dc.creator.none.fl_str_mv Serradell, Marianela del Carmen.
Saura, Alicia
Rupil, Lucia
Gargantini, Pablo Ruben
Faya, Marcela Inés
Furlan, Paulina
Lujan, Hugo Daniel
author Serradell, Marianela del Carmen.
author_facet Serradell, Marianela del Carmen.
Saura, Alicia
Rupil, Lucia
Gargantini, Pablo Ruben
Faya, Marcela Inés
Furlan, Paulina
Lujan, Hugo Daniel
author_role author
author2 Saura, Alicia
Rupil, Lucia
Gargantini, Pablo Ruben
Faya, Marcela Inés
Furlan, Paulina
Lujan, Hugo Daniel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PARASITIC INFECTION
PROTEIN VACCINES
topic PARASITIC INFECTION
PROTEIN VACCINES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Giardia lamblia is a human intestinal parasite and one of the most frequent enteric pathogen of companion animals. Clinical manifestations of giardiasis, such as diarrhoea, anorexia, weight loss and lethargy, have been associated with Giardia infections in both domestic and farm animals. A few anti-parasitic drugs are routinely used to treat giardiasis, but re-infections are common and drug-resistant strains have already been reported. Unfortunately, efficient vaccines against Giardia are not available. Giardia undergoes antigenic variation; through this mechanism, parasites can avoid the host?s immune defenses, causing chronic infections and/or re-infections. Antigenic variation is characterised by a continuous switch in the expression of members of a homologous family of genes encoding surface antigens. In a previous report, we indicated that in Giardia, the mechanism responsible for the exchange of variant-specific surface proteins (VSPs) involves the RNA interference (RNAi) pathway. From a repertoire of ~200 VSP genes, only one is expressed on the surface of single trophozoites; however, RNAi machinery disruption generates trophozoites that express the complete VSP repertoire. We also demonstrated that gerbils orally immunised with VSPs isolated from these altered parasites showed high levels of protection. Here we tested this vaccine in cats and dogs, and found that it is highly efficient in preventing new infections and reducing chronic giardiasis in domestic animals both in experimental and natural infections. Remarkably, immunisation of dogs in a highly endemic area strongly decreased the percentage of infected children in the community, suggesting that this vaccine would block the zoonotic transmission of the disease.
Fil: Serradell, Marianela del Carmen.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; Argentina
Fil: Saura, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; Argentina
Fil: Rupil, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; Argentina
Fil: Gargantini, Pablo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; Argentina
Fil: Faya, Marcela Inés. Universidad Católica de Córdoba; Argentina
Fil: Furlan, Paulina. Universidad Católica de Córdoba; Argentina
Fil: Lujan, Hugo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas. Universidad Catolica de Córdoba. Centro de Investigación y Desarrollo En Inmunología y Enfermedades Infecciosas; Argentina
description Giardia lamblia is a human intestinal parasite and one of the most frequent enteric pathogen of companion animals. Clinical manifestations of giardiasis, such as diarrhoea, anorexia, weight loss and lethargy, have been associated with Giardia infections in both domestic and farm animals. A few anti-parasitic drugs are routinely used to treat giardiasis, but re-infections are common and drug-resistant strains have already been reported. Unfortunately, efficient vaccines against Giardia are not available. Giardia undergoes antigenic variation; through this mechanism, parasites can avoid the host?s immune defenses, causing chronic infections and/or re-infections. Antigenic variation is characterised by a continuous switch in the expression of members of a homologous family of genes encoding surface antigens. In a previous report, we indicated that in Giardia, the mechanism responsible for the exchange of variant-specific surface proteins (VSPs) involves the RNA interference (RNAi) pathway. From a repertoire of ~200 VSP genes, only one is expressed on the surface of single trophozoites; however, RNAi machinery disruption generates trophozoites that express the complete VSP repertoire. We also demonstrated that gerbils orally immunised with VSPs isolated from these altered parasites showed high levels of protection. Here we tested this vaccine in cats and dogs, and found that it is highly efficient in preventing new infections and reducing chronic giardiasis in domestic animals both in experimental and natural infections. Remarkably, immunisation of dogs in a highly endemic area strongly decreased the percentage of infected children in the community, suggesting that this vaccine would block the zoonotic transmission of the disease.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40645
Serradell, Marianela del Carmen.; Saura, Alicia; Rupil, Lucia; Gargantini, Pablo Ruben; Faya, Marcela Inés; et al.; Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans; Springer Nature; npj vaccines; 9-2016; 1-11
2059-0105
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40645
identifier_str_mv Serradell, Marianela del Carmen.; Saura, Alicia; Rupil, Lucia; Gargantini, Pablo Ruben; Faya, Marcela Inés; et al.; Vaccination of domestic animals with a novel oral vaccine prevents Giardia infections, alleviates signs of giardiasis and reduces transmission to humans; Springer Nature; npj vaccines; 9-2016; 1-11
2059-0105
CONICET Digital
CONICET
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info:eu-repo/semantics/altIdentifier/doi/10.1038/npjvaccines.2016.18
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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application/pdf
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dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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