Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films

Autores
Funes, Matías Daniel; Caminos, Daniel Alberto; Alvarez, María Gabriela; Fungo, Fernando Gabriel; Otero, Luis Alberto; Durantini, Edgardo Néstor
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Spectroscopic and photodynamic properties of polymeric films bearing porphyrin units have been studied in both solution containing photooxidizable substrates and in vitro on Escherichia coli and Candida albicans microorganisms. The films were formed by electrochemical polymerization of 5,10,15,20-tetra(4- N,N-diphenylaminophenyl)porphyrin (H2P-film) and its complex with Pd(ll) (PdP-film) on optically transparent indium tin oxide (ITO) electrodes. Absorption spectroscopic studies show the characteristic Soret and Q bands of the porphyrin in the visible region and a band at ∼350 nm corresponding to the tetraphenylbenzidine units. Upon excitation, the H2P-film exhibits two bands of fluorescence emission from porphyrin, while it is not detected using PdP-film. The singlet molecular oxygen, O2( 1δg), productions of these surfaces were evaluated using 9,10-dimethylanthracene in N,N-dimethylformamide. Also, the photodynamic activity was compared in solutions of l-tryptophan. Under these conditions, oxidation of these substrates takes place indicating an efficient photodynamic action of both polymeric films. In vitro investigations show that these films produce photosensitized inactivation of microbial cells in aqueous suspensions. These films exhibit a photosensitizing activity causing a ∼3 log decrease of coli and ∼2.5 log of C. albicans cellular survival after 30 min of irradiation with visible light. The photodynamic effect of the surfaces was also tested by growth delay experiments. The results indicate that porphyrins immobilized on electropolymeric films are interesting and versatile photodynamic surfaces to inactivate microorganisms in liquid suspensions.
Fil: Funes, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Caminos, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Alvarez, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Fungo, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Otero, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Durantini, Edgardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Materia
Polymeric Films
Porphyrin
Photosensitisation
Antimicrobial
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/76838
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric filmsFunes, Matías DanielCaminos, Daniel AlbertoAlvarez, María GabrielaFungo, Fernando GabrielOtero, Luis AlbertoDurantini, Edgardo NéstorPolymeric FilmsPorphyrinPhotosensitisationAntimicrobialhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Spectroscopic and photodynamic properties of polymeric films bearing porphyrin units have been studied in both solution containing photooxidizable substrates and in vitro on Escherichia coli and Candida albicans microorganisms. The films were formed by electrochemical polymerization of 5,10,15,20-tetra(4- N,N-diphenylaminophenyl)porphyrin (H2P-film) and its complex with Pd(ll) (PdP-film) on optically transparent indium tin oxide (ITO) electrodes. Absorption spectroscopic studies show the characteristic Soret and Q bands of the porphyrin in the visible region and a band at ∼350 nm corresponding to the tetraphenylbenzidine units. Upon excitation, the H2P-film exhibits two bands of fluorescence emission from porphyrin, while it is not detected using PdP-film. The singlet molecular oxygen, O2( 1δg), productions of these surfaces were evaluated using 9,10-dimethylanthracene in N,N-dimethylformamide. Also, the photodynamic activity was compared in solutions of l-tryptophan. Under these conditions, oxidation of these substrates takes place indicating an efficient photodynamic action of both polymeric films. In vitro investigations show that these films produce photosensitized inactivation of microbial cells in aqueous suspensions. These films exhibit a photosensitizing activity causing a ∼3 log decrease of coli and ∼2.5 log of C. albicans cellular survival after 30 min of irradiation with visible light. The photodynamic effect of the surfaces was also tested by growth delay experiments. The results indicate that porphyrins immobilized on electropolymeric films are interesting and versatile photodynamic surfaces to inactivate microorganisms in liquid suspensions.Fil: Funes, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Caminos, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Alvarez, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Fungo, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Otero, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Durantini, Edgardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaAmerican Chemical Society2009-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76838Funes, Matías Daniel; Caminos, Daniel Alberto; Alvarez, María Gabriela; Fungo, Fernando Gabriel; Otero, Luis Alberto; et al.; Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films; American Chemical Society; Environmental Science & Technology; 43; 3; 2-2009; 902-9080013-936X1520-5851CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/es802450binfo:eu-repo/semantics/altIdentifier/doi/10.1021/es802450binfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:02Zoai:ri.conicet.gov.ar:11336/76838instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:02.385CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films
title Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films
spellingShingle Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films
Funes, Matías Daniel
Polymeric Films
Porphyrin
Photosensitisation
Antimicrobial
title_short Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films
title_full Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films
title_fullStr Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films
title_full_unstemmed Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films
title_sort Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films
dc.creator.none.fl_str_mv Funes, Matías Daniel
Caminos, Daniel Alberto
Alvarez, María Gabriela
Fungo, Fernando Gabriel
Otero, Luis Alberto
Durantini, Edgardo Néstor
author Funes, Matías Daniel
author_facet Funes, Matías Daniel
Caminos, Daniel Alberto
Alvarez, María Gabriela
Fungo, Fernando Gabriel
Otero, Luis Alberto
Durantini, Edgardo Néstor
author_role author
author2 Caminos, Daniel Alberto
Alvarez, María Gabriela
Fungo, Fernando Gabriel
Otero, Luis Alberto
Durantini, Edgardo Néstor
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Polymeric Films
Porphyrin
Photosensitisation
Antimicrobial
topic Polymeric Films
Porphyrin
Photosensitisation
Antimicrobial
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Spectroscopic and photodynamic properties of polymeric films bearing porphyrin units have been studied in both solution containing photooxidizable substrates and in vitro on Escherichia coli and Candida albicans microorganisms. The films were formed by electrochemical polymerization of 5,10,15,20-tetra(4- N,N-diphenylaminophenyl)porphyrin (H2P-film) and its complex with Pd(ll) (PdP-film) on optically transparent indium tin oxide (ITO) electrodes. Absorption spectroscopic studies show the characteristic Soret and Q bands of the porphyrin in the visible region and a band at ∼350 nm corresponding to the tetraphenylbenzidine units. Upon excitation, the H2P-film exhibits two bands of fluorescence emission from porphyrin, while it is not detected using PdP-film. The singlet molecular oxygen, O2( 1δg), productions of these surfaces were evaluated using 9,10-dimethylanthracene in N,N-dimethylformamide. Also, the photodynamic activity was compared in solutions of l-tryptophan. Under these conditions, oxidation of these substrates takes place indicating an efficient photodynamic action of both polymeric films. In vitro investigations show that these films produce photosensitized inactivation of microbial cells in aqueous suspensions. These films exhibit a photosensitizing activity causing a ∼3 log decrease of coli and ∼2.5 log of C. albicans cellular survival after 30 min of irradiation with visible light. The photodynamic effect of the surfaces was also tested by growth delay experiments. The results indicate that porphyrins immobilized on electropolymeric films are interesting and versatile photodynamic surfaces to inactivate microorganisms in liquid suspensions.
Fil: Funes, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Caminos, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Alvarez, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Fungo, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Otero, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Durantini, Edgardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
description Spectroscopic and photodynamic properties of polymeric films bearing porphyrin units have been studied in both solution containing photooxidizable substrates and in vitro on Escherichia coli and Candida albicans microorganisms. The films were formed by electrochemical polymerization of 5,10,15,20-tetra(4- N,N-diphenylaminophenyl)porphyrin (H2P-film) and its complex with Pd(ll) (PdP-film) on optically transparent indium tin oxide (ITO) electrodes. Absorption spectroscopic studies show the characteristic Soret and Q bands of the porphyrin in the visible region and a band at ∼350 nm corresponding to the tetraphenylbenzidine units. Upon excitation, the H2P-film exhibits two bands of fluorescence emission from porphyrin, while it is not detected using PdP-film. The singlet molecular oxygen, O2( 1δg), productions of these surfaces were evaluated using 9,10-dimethylanthracene in N,N-dimethylformamide. Also, the photodynamic activity was compared in solutions of l-tryptophan. Under these conditions, oxidation of these substrates takes place indicating an efficient photodynamic action of both polymeric films. In vitro investigations show that these films produce photosensitized inactivation of microbial cells in aqueous suspensions. These films exhibit a photosensitizing activity causing a ∼3 log decrease of coli and ∼2.5 log of C. albicans cellular survival after 30 min of irradiation with visible light. The photodynamic effect of the surfaces was also tested by growth delay experiments. The results indicate that porphyrins immobilized on electropolymeric films are interesting and versatile photodynamic surfaces to inactivate microorganisms in liquid suspensions.
publishDate 2009
dc.date.none.fl_str_mv 2009-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/76838
Funes, Matías Daniel; Caminos, Daniel Alberto; Alvarez, María Gabriela; Fungo, Fernando Gabriel; Otero, Luis Alberto; et al.; Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films; American Chemical Society; Environmental Science & Technology; 43; 3; 2-2009; 902-908
0013-936X
1520-5851
CONICET Digital
CONICET
url http://hdl.handle.net/11336/76838
identifier_str_mv Funes, Matías Daniel; Caminos, Daniel Alberto; Alvarez, María Gabriela; Fungo, Fernando Gabriel; Otero, Luis Alberto; et al.; Photodynamic properties and photoantimicrobial action of electrochemically generated porphyrin polymeric films; American Chemical Society; Environmental Science & Technology; 43; 3; 2-2009; 902-908
0013-936X
1520-5851
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1021/es802450b
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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