Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum

Autores
Aguilera, Patricia Mabel; Sartor, Maria Esperanza; Galdeano, Florencia; Espinoza, Francisco; Quarin, Camilo Luis
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Plicatula group of the grass genus Paspalum contains about 30 species. Most are tetraploid and reproduce by apomixis, though some of them contain sexual diploid races. We crossed a sexual colchicine-induced autotetraploid plant of brownseed paspalum (Paspalum plicatulum Michx.) with apomictic tetraploid P. guenoarum Arechav., also from the Plicatula group. Crossability was 35% and the progeny showed morphological characteristics intermediate to those of the parents but resembling more the male parent. The random amplifi ed polymorphic DNA (RAPD) analysis showed that the whole progeny amplifi ed bands that were specifi c of the male parent, confi rming its hybrid origin. Meiotic chromosome behavior of hybrids exhibited primarily bivalent and quadrivalent associations similar to those of the two parents. This suggests that natural tetraploid P. guenoarum shares the same basic chromosome set with that of autotetraploid P. plicatulum, and both species probably originate from the same ancestral species. Fourteen out of 23 hybrids reproduced only sexually, while nine were obligate or highly apomictic. Seed set ranged from 11 to 55% among the hybrids. Our results open the possibility of exchanging genes at the tetraploid level in breeding programs of P. plicatulum and P. guenoarum. This possibility is based on their rate of crossability, the degree of fertility among the hybrids, the segregation observed for the reproductive mode in the F1 progeny, and the very simple procedure fl ow cytometry seed screen (FCSS) used to determine the reproductive mode for each hybrid.
Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Sartor, Maria Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Materia
Plicatula
Apomixis
Hybrids
Fcss
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2953
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2953
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarumAguilera, Patricia MabelSartor, Maria EsperanzaGaldeano, FlorenciaEspinoza, FranciscoQuarin, Camilo LuisPlicatulaApomixisHybridsFcsshttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The Plicatula group of the grass genus Paspalum contains about 30 species. Most are tetraploid and reproduce by apomixis, though some of them contain sexual diploid races. We crossed a sexual colchicine-induced autotetraploid plant of brownseed paspalum (Paspalum plicatulum Michx.) with apomictic tetraploid P. guenoarum Arechav., also from the Plicatula group. Crossability was 35% and the progeny showed morphological characteristics intermediate to those of the parents but resembling more the male parent. The random amplifi ed polymorphic DNA (RAPD) analysis showed that the whole progeny amplifi ed bands that were specifi c of the male parent, confi rming its hybrid origin. Meiotic chromosome behavior of hybrids exhibited primarily bivalent and quadrivalent associations similar to those of the two parents. This suggests that natural tetraploid P. guenoarum shares the same basic chromosome set with that of autotetraploid P. plicatulum, and both species probably originate from the same ancestral species. Fourteen out of 23 hybrids reproduced only sexually, while nine were obligate or highly apomictic. Seed set ranged from 11 to 55% among the hybrids. Our results open the possibility of exchanging genes at the tetraploid level in breeding programs of P. plicatulum and P. guenoarum. This possibility is based on their rate of crossability, the degree of fertility among the hybrids, the segregation observed for the reproductive mode in the F1 progeny, and the very simple procedure fl ow cytometry seed screen (FCSS) used to determine the reproductive mode for each hybrid.Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Sartor, Maria Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaCrop Science Soc Amer2011-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2953Aguilera, Patricia Mabel; Sartor, Maria Esperanza; Galdeano, Florencia; Espinoza, Francisco; Quarin, Camilo Luis; Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum; Crop Science Soc Amer; Crop Science; 51; 4; 7-2011; 1544-15500011-183Xenginfo:eu-repo/semantics/altIdentifier/url/https://dl.sciencesocieties.org/publications/cs/abstracts/51/4/1544info:eu-repo/semantics/altIdentifier/url/https://www.crops.org/publications/cs/abstracts/51/4/1544?access=0&view=pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:23Zoai:ri.conicet.gov.ar:11336/2953instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:23.606CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum
title Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum
spellingShingle Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum
Aguilera, Patricia Mabel
Plicatula
Apomixis
Hybrids
Fcss
title_short Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum
title_full Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum
title_fullStr Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum
title_full_unstemmed Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum
title_sort Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum
dc.creator.none.fl_str_mv Aguilera, Patricia Mabel
Sartor, Maria Esperanza
Galdeano, Florencia
Espinoza, Francisco
Quarin, Camilo Luis
author Aguilera, Patricia Mabel
author_facet Aguilera, Patricia Mabel
Sartor, Maria Esperanza
Galdeano, Florencia
Espinoza, Francisco
Quarin, Camilo Luis
author_role author
author2 Sartor, Maria Esperanza
Galdeano, Florencia
Espinoza, Francisco
Quarin, Camilo Luis
author2_role author
author
author
author
dc.subject.none.fl_str_mv Plicatula
Apomixis
Hybrids
Fcss
topic Plicatula
Apomixis
Hybrids
Fcss
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The Plicatula group of the grass genus Paspalum contains about 30 species. Most are tetraploid and reproduce by apomixis, though some of them contain sexual diploid races. We crossed a sexual colchicine-induced autotetraploid plant of brownseed paspalum (Paspalum plicatulum Michx.) with apomictic tetraploid P. guenoarum Arechav., also from the Plicatula group. Crossability was 35% and the progeny showed morphological characteristics intermediate to those of the parents but resembling more the male parent. The random amplifi ed polymorphic DNA (RAPD) analysis showed that the whole progeny amplifi ed bands that were specifi c of the male parent, confi rming its hybrid origin. Meiotic chromosome behavior of hybrids exhibited primarily bivalent and quadrivalent associations similar to those of the two parents. This suggests that natural tetraploid P. guenoarum shares the same basic chromosome set with that of autotetraploid P. plicatulum, and both species probably originate from the same ancestral species. Fourteen out of 23 hybrids reproduced only sexually, while nine were obligate or highly apomictic. Seed set ranged from 11 to 55% among the hybrids. Our results open the possibility of exchanging genes at the tetraploid level in breeding programs of P. plicatulum and P. guenoarum. This possibility is based on their rate of crossability, the degree of fertility among the hybrids, the segregation observed for the reproductive mode in the F1 progeny, and the very simple procedure fl ow cytometry seed screen (FCSS) used to determine the reproductive mode for each hybrid.
Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Sartor, Maria Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
description The Plicatula group of the grass genus Paspalum contains about 30 species. Most are tetraploid and reproduce by apomixis, though some of them contain sexual diploid races. We crossed a sexual colchicine-induced autotetraploid plant of brownseed paspalum (Paspalum plicatulum Michx.) with apomictic tetraploid P. guenoarum Arechav., also from the Plicatula group. Crossability was 35% and the progeny showed morphological characteristics intermediate to those of the parents but resembling more the male parent. The random amplifi ed polymorphic DNA (RAPD) analysis showed that the whole progeny amplifi ed bands that were specifi c of the male parent, confi rming its hybrid origin. Meiotic chromosome behavior of hybrids exhibited primarily bivalent and quadrivalent associations similar to those of the two parents. This suggests that natural tetraploid P. guenoarum shares the same basic chromosome set with that of autotetraploid P. plicatulum, and both species probably originate from the same ancestral species. Fourteen out of 23 hybrids reproduced only sexually, while nine were obligate or highly apomictic. Seed set ranged from 11 to 55% among the hybrids. Our results open the possibility of exchanging genes at the tetraploid level in breeding programs of P. plicatulum and P. guenoarum. This possibility is based on their rate of crossability, the degree of fertility among the hybrids, the segregation observed for the reproductive mode in the F1 progeny, and the very simple procedure fl ow cytometry seed screen (FCSS) used to determine the reproductive mode for each hybrid.
publishDate 2011
dc.date.none.fl_str_mv 2011-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2953
Aguilera, Patricia Mabel; Sartor, Maria Esperanza; Galdeano, Florencia; Espinoza, Francisco; Quarin, Camilo Luis; Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum; Crop Science Soc Amer; Crop Science; 51; 4; 7-2011; 1544-1550
0011-183X
url http://hdl.handle.net/11336/2953
identifier_str_mv Aguilera, Patricia Mabel; Sartor, Maria Esperanza; Galdeano, Florencia; Espinoza, Francisco; Quarin, Camilo Luis; Interspecific tetraploid hybrids between two forage grass species: sexual Paspalum plicatulum and apomictic P. guenoarum; Crop Science Soc Amer; Crop Science; 51; 4; 7-2011; 1544-1550
0011-183X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://dl.sciencesocieties.org/publications/cs/abstracts/51/4/1544
info:eu-repo/semantics/altIdentifier/url/https://www.crops.org/publications/cs/abstracts/51/4/1544?access=0&view=pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Crop Science Soc Amer
publisher.none.fl_str_mv Crop Science Soc Amer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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