A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments

Autores
Chathirat, Naphat; Atthi, Nithi; Hruanun, Charndet; Poyai, Amporn; Leasen, Suthisa; Osotchan, Tanakorn; Hodak, Jose Hector
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A biosensor structure comprising silicon nitride (Si3N 4) micrograting arrays coated with a spin-on-glass (SOG) material was investigated. This grating structure was located on a silicon groove, which was etched by a deep reactive ion etching (DRIE) process. The biosensor was used as a specific detector of DNA molecules and antibody-antigen interactions. In our DNA sensing experiments, the first step was the activation of the grating surface with amine functional groups, followed by attachment of a 23-base oligonucleotide probe layer for hybridization with a complementary target DNA. The sensing device was tested for detecting specific antigen/antibody interactions for human serum albumin (HSA) and antigen bovine serum albumin (BSA). The readout system consisted of a white light lamp that illuminated a small spot on the grating surface at normal incidence through a fiber optic probe with a spectrometer used to collect the reflected light through a second fiber. We show that these sensing devices have the capability to detect DNA as well as antigen-antibody binding for HSA. The detection sensitivity for HSA was better than that for DNA mainly owing to the larger size and concomitant refractive index changes upon binding to the sensor. We show that it is possible to quantify the amount of biomolecules bound to the grating surface by measuring the wavelength shift of the reflectance spectra upon exposure to the samples.
Fil: Chathirat, Naphat. Mahidol University; Tailandia
Fil: Atthi, Nithi. Thailand National Electronics and Computer Technology Center; Tailandia
Fil: Hruanun, Charndet. Thailand National Electronics and Computer Technology Center; Tailandia
Fil: Poyai, Amporn. Thailand National Electronics and Computer Technology Center; Tailandia
Fil: Leasen, Suthisa. Thailand National Electronics and Computer Technology Center; Tailandia
Fil: Osotchan, Tanakorn. Thailand National Electronics and Computer Technology Center; Tailandia
Fil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Materia
Micrograting Sensor
Dna Hybridization
Biosensor
Antibody
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71958
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/71958
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experimentsChathirat, NaphatAtthi, NithiHruanun, CharndetPoyai, AmpornLeasen, SuthisaOsotchan, TanakornHodak, Jose HectorMicrograting SensorDna HybridizationBiosensorAntibodyhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A biosensor structure comprising silicon nitride (Si3N 4) micrograting arrays coated with a spin-on-glass (SOG) material was investigated. This grating structure was located on a silicon groove, which was etched by a deep reactive ion etching (DRIE) process. The biosensor was used as a specific detector of DNA molecules and antibody-antigen interactions. In our DNA sensing experiments, the first step was the activation of the grating surface with amine functional groups, followed by attachment of a 23-base oligonucleotide probe layer for hybridization with a complementary target DNA. The sensing device was tested for detecting specific antigen/antibody interactions for human serum albumin (HSA) and antigen bovine serum albumin (BSA). The readout system consisted of a white light lamp that illuminated a small spot on the grating surface at normal incidence through a fiber optic probe with a spectrometer used to collect the reflected light through a second fiber. We show that these sensing devices have the capability to detect DNA as well as antigen-antibody binding for HSA. The detection sensitivity for HSA was better than that for DNA mainly owing to the larger size and concomitant refractive index changes upon binding to the sensor. We show that it is possible to quantify the amount of biomolecules bound to the grating surface by measuring the wavelength shift of the reflectance spectra upon exposure to the samples.Fil: Chathirat, Naphat. Mahidol University; TailandiaFil: Atthi, Nithi. Thailand National Electronics and Computer Technology Center; TailandiaFil: Hruanun, Charndet. Thailand National Electronics and Computer Technology Center; TailandiaFil: Poyai, Amporn. Thailand National Electronics and Computer Technology Center; TailandiaFil: Leasen, Suthisa. Thailand National Electronics and Computer Technology Center; TailandiaFil: Osotchan, Tanakorn. Thailand National Electronics and Computer Technology Center; TailandiaFil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaJapan Society Applied Physics2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71958Chathirat, Naphat; Atthi, Nithi; Hruanun, Charndet; Poyai, Amporn; Leasen, Suthisa; et al.; A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments; Japan Society Applied Physics; Japanese Journal Of Applied Physics; 50; 1 PART 3; 1-20110021-4922CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1143/JJAP.50.01BK01info:eu-repo/semantics/altIdentifier/doi/10.1143/JJAP.50.01BK01info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:38:54Zoai:ri.conicet.gov.ar:11336/71958instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:38:54.662CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments
title A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments
spellingShingle A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments
Chathirat, Naphat
Micrograting Sensor
Dna Hybridization
Biosensor
Antibody
title_short A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments
title_full A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments
title_fullStr A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments
title_full_unstemmed A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments
title_sort A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments
dc.creator.none.fl_str_mv Chathirat, Naphat
Atthi, Nithi
Hruanun, Charndet
Poyai, Amporn
Leasen, Suthisa
Osotchan, Tanakorn
Hodak, Jose Hector
author Chathirat, Naphat
author_facet Chathirat, Naphat
Atthi, Nithi
Hruanun, Charndet
Poyai, Amporn
Leasen, Suthisa
Osotchan, Tanakorn
Hodak, Jose Hector
author_role author
author2 Atthi, Nithi
Hruanun, Charndet
Poyai, Amporn
Leasen, Suthisa
Osotchan, Tanakorn
Hodak, Jose Hector
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Micrograting Sensor
Dna Hybridization
Biosensor
Antibody
topic Micrograting Sensor
Dna Hybridization
Biosensor
Antibody
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A biosensor structure comprising silicon nitride (Si3N 4) micrograting arrays coated with a spin-on-glass (SOG) material was investigated. This grating structure was located on a silicon groove, which was etched by a deep reactive ion etching (DRIE) process. The biosensor was used as a specific detector of DNA molecules and antibody-antigen interactions. In our DNA sensing experiments, the first step was the activation of the grating surface with amine functional groups, followed by attachment of a 23-base oligonucleotide probe layer for hybridization with a complementary target DNA. The sensing device was tested for detecting specific antigen/antibody interactions for human serum albumin (HSA) and antigen bovine serum albumin (BSA). The readout system consisted of a white light lamp that illuminated a small spot on the grating surface at normal incidence through a fiber optic probe with a spectrometer used to collect the reflected light through a second fiber. We show that these sensing devices have the capability to detect DNA as well as antigen-antibody binding for HSA. The detection sensitivity for HSA was better than that for DNA mainly owing to the larger size and concomitant refractive index changes upon binding to the sensor. We show that it is possible to quantify the amount of biomolecules bound to the grating surface by measuring the wavelength shift of the reflectance spectra upon exposure to the samples.
Fil: Chathirat, Naphat. Mahidol University; Tailandia
Fil: Atthi, Nithi. Thailand National Electronics and Computer Technology Center; Tailandia
Fil: Hruanun, Charndet. Thailand National Electronics and Computer Technology Center; Tailandia
Fil: Poyai, Amporn. Thailand National Electronics and Computer Technology Center; Tailandia
Fil: Leasen, Suthisa. Thailand National Electronics and Computer Technology Center; Tailandia
Fil: Osotchan, Tanakorn. Thailand National Electronics and Computer Technology Center; Tailandia
Fil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
description A biosensor structure comprising silicon nitride (Si3N 4) micrograting arrays coated with a spin-on-glass (SOG) material was investigated. This grating structure was located on a silicon groove, which was etched by a deep reactive ion etching (DRIE) process. The biosensor was used as a specific detector of DNA molecules and antibody-antigen interactions. In our DNA sensing experiments, the first step was the activation of the grating surface with amine functional groups, followed by attachment of a 23-base oligonucleotide probe layer for hybridization with a complementary target DNA. The sensing device was tested for detecting specific antigen/antibody interactions for human serum albumin (HSA) and antigen bovine serum albumin (BSA). The readout system consisted of a white light lamp that illuminated a small spot on the grating surface at normal incidence through a fiber optic probe with a spectrometer used to collect the reflected light through a second fiber. We show that these sensing devices have the capability to detect DNA as well as antigen-antibody binding for HSA. The detection sensitivity for HSA was better than that for DNA mainly owing to the larger size and concomitant refractive index changes upon binding to the sensor. We show that it is possible to quantify the amount of biomolecules bound to the grating surface by measuring the wavelength shift of the reflectance spectra upon exposure to the samples.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71958
Chathirat, Naphat; Atthi, Nithi; Hruanun, Charndet; Poyai, Amporn; Leasen, Suthisa; et al.; A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments; Japan Society Applied Physics; Japanese Journal Of Applied Physics; 50; 1 PART 3; 1-2011
0021-4922
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71958
identifier_str_mv Chathirat, Naphat; Atthi, Nithi; Hruanun, Charndet; Poyai, Amporn; Leasen, Suthisa; et al.; A micrograting sensor for DNA hybridization and antibody human serum Albumin-Antigen human serum albumin interaction experiments; Japan Society Applied Physics; Japanese Journal Of Applied Physics; 50; 1 PART 3; 1-2011
0021-4922
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1143/JJAP.50.01BK01
info:eu-repo/semantics/altIdentifier/doi/10.1143/JJAP.50.01BK01
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Japan Society Applied Physics
publisher.none.fl_str_mv Japan Society Applied Physics
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083506590973952
score 13.22299

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