Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol

Autores
Scaia, María Florencia; Regueira, Eleonora; Volonteri, María Clara; Ceballos, Nora Raquel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In bufonids, the Bidder´s organ (BO), located in the anterior pole of the testis, is sometimes referred to as a rudimentary ovary because of the presence of previtellogenic follicles. In males of Rhinella arenarum it has been demonstrated that some follicles are vitellogenic and also express several steroidogenic enzymes in follicular cells. The purpose of this study is to describe seasonal variations in plasma estradiol (E2) and in aromatase activity of the BO, and to determine the capacity of the BO to synthesize E2 from cholesterol in males of R. arenarum. E2 was determined by radioimmunoassay and aromatase activity was measured by transformation of radioactive substrates into products. Results indicate that plasma E2 reached the highest concentration in April and the lowest one in animals captured in June, showing a progressive increase to the end of the year. Plasma E2 and total activity of aromatase in the BO were significantly lower during the pre‐reproductive season than during the reproductive and post‐reproductive seasons. It was also demonstrated that the BO is able to produce E2 from endogenous substrates throughout the year. No correlation was found between plasma E2 and total BO weight, while there was a significant correlation between plasma E2 and total activity of aromatase, and between plasma E2 and E2 produced in vitro. Taken together, these results demonstrate seasonal variations in plasma E2, in bidderian total activity of aromatase and, that the BO of adult males of R. arenarum is able to produce E2 from endogenous substrates.
Fil: Scaia, María Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Regueira, Eleonora. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Volonteri, María Clara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceballos, Nora Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Bidder´S Organ
Toad
Estradiol
Aromatase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24059
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24059
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiolScaia, María FlorenciaRegueira, EleonoraVolonteri, María ClaraCeballos, Nora RaquelBidder´S OrganToadEstradiolAromatasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In bufonids, the Bidder´s organ (BO), located in the anterior pole of the testis, is sometimes referred to as a rudimentary ovary because of the presence of previtellogenic follicles. In males of Rhinella arenarum it has been demonstrated that some follicles are vitellogenic and also express several steroidogenic enzymes in follicular cells. The purpose of this study is to describe seasonal variations in plasma estradiol (E2) and in aromatase activity of the BO, and to determine the capacity of the BO to synthesize E2 from cholesterol in males of R. arenarum. E2 was determined by radioimmunoassay and aromatase activity was measured by transformation of radioactive substrates into products. Results indicate that plasma E2 reached the highest concentration in April and the lowest one in animals captured in June, showing a progressive increase to the end of the year. Plasma E2 and total activity of aromatase in the BO were significantly lower during the pre‐reproductive season than during the reproductive and post‐reproductive seasons. It was also demonstrated that the BO is able to produce E2 from endogenous substrates throughout the year. No correlation was found between plasma E2 and total BO weight, while there was a significant correlation between plasma E2 and total activity of aromatase, and between plasma E2 and E2 produced in vitro. Taken together, these results demonstrate seasonal variations in plasma E2, in bidderian total activity of aromatase and, that the BO of adult males of R. arenarum is able to produce E2 from endogenous substrates.Fil: Scaia, María Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Regueira, Eleonora. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Volonteri, María Clara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ceballos, Nora Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24059Scaia, María Florencia; Regueira, Eleonora; Volonteri, María Clara; Ceballos, Nora Raquel; Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol; Wiley; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 319; 6; 5-2013; 355-3641932-52232471-5646CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jez.1799/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/jez.1799info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:28Zoai:ri.conicet.gov.ar:11336/24059instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:28.907CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol
title Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol
spellingShingle Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol
Scaia, María Florencia
Bidder´S Organ
Toad
Estradiol
Aromatase
title_short Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol
title_full Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol
title_fullStr Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol
title_full_unstemmed Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol
title_sort Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol
dc.creator.none.fl_str_mv Scaia, María Florencia
Regueira, Eleonora
Volonteri, María Clara
Ceballos, Nora Raquel
author Scaia, María Florencia
author_facet Scaia, María Florencia
Regueira, Eleonora
Volonteri, María Clara
Ceballos, Nora Raquel
author_role author
author2 Regueira, Eleonora
Volonteri, María Clara
Ceballos, Nora Raquel
author2_role author
author
author
dc.subject.none.fl_str_mv Bidder´S Organ
Toad
Estradiol
Aromatase
topic Bidder´S Organ
Toad
Estradiol
Aromatase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In bufonids, the Bidder´s organ (BO), located in the anterior pole of the testis, is sometimes referred to as a rudimentary ovary because of the presence of previtellogenic follicles. In males of Rhinella arenarum it has been demonstrated that some follicles are vitellogenic and also express several steroidogenic enzymes in follicular cells. The purpose of this study is to describe seasonal variations in plasma estradiol (E2) and in aromatase activity of the BO, and to determine the capacity of the BO to synthesize E2 from cholesterol in males of R. arenarum. E2 was determined by radioimmunoassay and aromatase activity was measured by transformation of radioactive substrates into products. Results indicate that plasma E2 reached the highest concentration in April and the lowest one in animals captured in June, showing a progressive increase to the end of the year. Plasma E2 and total activity of aromatase in the BO were significantly lower during the pre‐reproductive season than during the reproductive and post‐reproductive seasons. It was also demonstrated that the BO is able to produce E2 from endogenous substrates throughout the year. No correlation was found between plasma E2 and total BO weight, while there was a significant correlation between plasma E2 and total activity of aromatase, and between plasma E2 and E2 produced in vitro. Taken together, these results demonstrate seasonal variations in plasma E2, in bidderian total activity of aromatase and, that the BO of adult males of R. arenarum is able to produce E2 from endogenous substrates.
Fil: Scaia, María Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Regueira, Eleonora. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Volonteri, María Clara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceballos, Nora Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Endocrinología Comparada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In bufonids, the Bidder´s organ (BO), located in the anterior pole of the testis, is sometimes referred to as a rudimentary ovary because of the presence of previtellogenic follicles. In males of Rhinella arenarum it has been demonstrated that some follicles are vitellogenic and also express several steroidogenic enzymes in follicular cells. The purpose of this study is to describe seasonal variations in plasma estradiol (E2) and in aromatase activity of the BO, and to determine the capacity of the BO to synthesize E2 from cholesterol in males of R. arenarum. E2 was determined by radioimmunoassay and aromatase activity was measured by transformation of radioactive substrates into products. Results indicate that plasma E2 reached the highest concentration in April and the lowest one in animals captured in June, showing a progressive increase to the end of the year. Plasma E2 and total activity of aromatase in the BO were significantly lower during the pre‐reproductive season than during the reproductive and post‐reproductive seasons. It was also demonstrated that the BO is able to produce E2 from endogenous substrates throughout the year. No correlation was found between plasma E2 and total BO weight, while there was a significant correlation between plasma E2 and total activity of aromatase, and between plasma E2 and E2 produced in vitro. Taken together, these results demonstrate seasonal variations in plasma E2, in bidderian total activity of aromatase and, that the BO of adult males of R. arenarum is able to produce E2 from endogenous substrates.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24059
Scaia, María Florencia; Regueira, Eleonora; Volonteri, María Clara; Ceballos, Nora Raquel; Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol; Wiley; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 319; 6; 5-2013; 355-364
1932-5223
2471-5646
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24059
identifier_str_mv Scaia, María Florencia; Regueira, Eleonora; Volonteri, María Clara; Ceballos, Nora Raquel; Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura): Seasonal variations in plasma estradiol; Wiley; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 319; 6; 5-2013; 355-364
1932-5223
2471-5646
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jez.1799/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/jez.1799
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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