Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells

Autores
Astiz, Mariana; Hurtado, Graciela Elisa; Tacconi, Maria Josefa; Marra, Carlos Alberto
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present work studies the potential restorative effect of polyunsaturated fatty acids (PUFAs, 5μM/24 hs) on the dimethoate (DMT)-induced inhibition of testosterone biosynthesis in Leydig cells isolated from rat testes. Various fatty acids (FAs) from the n-6 (18:2, 20:3, 20:4, 22:4 and 22:5) and n-3 (18.3, 20:5, 22:5, 22:6) series were assayed in Leydig cells, alone (as delipidated BSA complexes) and in combination with DMT (1 ppm). The n-6 FAs stimulated lipid peroxidation (LPO) and inhibited the activities of steroidogenic enzymes (3beta- and 17beta-hydroysteroid dehydrogenases). The n-3 FAs exerted an anti-oxidant effect, decreasing the production of thiobarbituric-acid reactive substances (TBARS) and inhibiting phospholipase A2 activity. 20:4n-6 (ARA), completely and 20:3n-6 partially, normalized the biosynthesis of testosterone in DMT-treated cultures, increasing ARA content inside the mitochondria, whereas the other FAs assayed failed to restore androgenesis. COX-2 protein and prostaglandin F2alfa and E2 production were stimulated by 20:3n-6, 20:4n-6, 18:3n-3 and 20:5 n-3. COX-2 protein decreased upon addition of 22:5n-3 and 22:6n-3. StAR protein was increased by 20:4n-6 and partially by 20:3n-6, likely due to its metabolic conversion into ARA. Both FAs increased the mitochondrial cholesterol pool available for testosterone biosynthesis. The rate of androgenesis is likely the result of various regulatory factors acting concomitantly on the physiology of Leydig cells.
Fil: Astiz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Hurtado, Graciela Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Tacconi, Maria Josefa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Marra, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Materia
STEROIDOGENESIS
ARACHIDONATE
LEYDIG CELLS
DIMETHOATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269437
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/269437
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial CellsAstiz, MarianaHurtado, Graciela ElisaTacconi, Maria JosefaMarra, Carlos AlbertoSTEROIDOGENESISARACHIDONATELEYDIG CELLSDIMETHOATEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The present work studies the potential restorative effect of polyunsaturated fatty acids (PUFAs, 5μM/24 hs) on the dimethoate (DMT)-induced inhibition of testosterone biosynthesis in Leydig cells isolated from rat testes. Various fatty acids (FAs) from the n-6 (18:2, 20:3, 20:4, 22:4 and 22:5) and n-3 (18.3, 20:5, 22:5, 22:6) series were assayed in Leydig cells, alone (as delipidated BSA complexes) and in combination with DMT (1 ppm). The n-6 FAs stimulated lipid peroxidation (LPO) and inhibited the activities of steroidogenic enzymes (3beta- and 17beta-hydroysteroid dehydrogenases). The n-3 FAs exerted an anti-oxidant effect, decreasing the production of thiobarbituric-acid reactive substances (TBARS) and inhibiting phospholipase A2 activity. 20:4n-6 (ARA), completely and 20:3n-6 partially, normalized the biosynthesis of testosterone in DMT-treated cultures, increasing ARA content inside the mitochondria, whereas the other FAs assayed failed to restore androgenesis. COX-2 protein and prostaglandin F2alfa and E2 production were stimulated by 20:3n-6, 20:4n-6, 18:3n-3 and 20:5 n-3. COX-2 protein decreased upon addition of 22:5n-3 and 22:6n-3. StAR protein was increased by 20:4n-6 and partially by 20:3n-6, likely due to its metabolic conversion into ARA. Both FAs increased the mitochondrial cholesterol pool available for testosterone biosynthesis. The rate of androgenesis is likely the result of various regulatory factors acting concomitantly on the physiology of Leydig cells.Fil: Astiz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Hurtado, Graciela Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Tacconi, Maria Josefa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Marra, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaSpringer Heidelberg2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269437Astiz, Mariana; Hurtado, Graciela Elisa; Tacconi, Maria Josefa; Marra, Carlos Alberto; Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells; Springer Heidelberg; Lipids; 47; 6; 4-2012; 557-5690024-4201CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11745-012-3669-yinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-012-3669-yinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:01Zoai:ri.conicet.gov.ar:11336/269437instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:01.716CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells
title Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells
spellingShingle Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells
Astiz, Mariana
STEROIDOGENESIS
ARACHIDONATE
LEYDIG CELLS
DIMETHOATE
title_short Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells
title_full Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells
title_fullStr Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells
title_full_unstemmed Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells
title_sort Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells
dc.creator.none.fl_str_mv Astiz, Mariana
Hurtado, Graciela Elisa
Tacconi, Maria Josefa
Marra, Carlos Alberto
author Astiz, Mariana
author_facet Astiz, Mariana
Hurtado, Graciela Elisa
Tacconi, Maria Josefa
Marra, Carlos Alberto
author_role author
author2 Hurtado, Graciela Elisa
Tacconi, Maria Josefa
Marra, Carlos Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv STEROIDOGENESIS
ARACHIDONATE
LEYDIG CELLS
DIMETHOATE
topic STEROIDOGENESIS
ARACHIDONATE
LEYDIG CELLS
DIMETHOATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The present work studies the potential restorative effect of polyunsaturated fatty acids (PUFAs, 5μM/24 hs) on the dimethoate (DMT)-induced inhibition of testosterone biosynthesis in Leydig cells isolated from rat testes. Various fatty acids (FAs) from the n-6 (18:2, 20:3, 20:4, 22:4 and 22:5) and n-3 (18.3, 20:5, 22:5, 22:6) series were assayed in Leydig cells, alone (as delipidated BSA complexes) and in combination with DMT (1 ppm). The n-6 FAs stimulated lipid peroxidation (LPO) and inhibited the activities of steroidogenic enzymes (3beta- and 17beta-hydroysteroid dehydrogenases). The n-3 FAs exerted an anti-oxidant effect, decreasing the production of thiobarbituric-acid reactive substances (TBARS) and inhibiting phospholipase A2 activity. 20:4n-6 (ARA), completely and 20:3n-6 partially, normalized the biosynthesis of testosterone in DMT-treated cultures, increasing ARA content inside the mitochondria, whereas the other FAs assayed failed to restore androgenesis. COX-2 protein and prostaglandin F2alfa and E2 production were stimulated by 20:3n-6, 20:4n-6, 18:3n-3 and 20:5 n-3. COX-2 protein decreased upon addition of 22:5n-3 and 22:6n-3. StAR protein was increased by 20:4n-6 and partially by 20:3n-6, likely due to its metabolic conversion into ARA. Both FAs increased the mitochondrial cholesterol pool available for testosterone biosynthesis. The rate of androgenesis is likely the result of various regulatory factors acting concomitantly on the physiology of Leydig cells.
Fil: Astiz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Hurtado, Graciela Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Tacconi, Maria Josefa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Marra, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
description The present work studies the potential restorative effect of polyunsaturated fatty acids (PUFAs, 5μM/24 hs) on the dimethoate (DMT)-induced inhibition of testosterone biosynthesis in Leydig cells isolated from rat testes. Various fatty acids (FAs) from the n-6 (18:2, 20:3, 20:4, 22:4 and 22:5) and n-3 (18.3, 20:5, 22:5, 22:6) series were assayed in Leydig cells, alone (as delipidated BSA complexes) and in combination with DMT (1 ppm). The n-6 FAs stimulated lipid peroxidation (LPO) and inhibited the activities of steroidogenic enzymes (3beta- and 17beta-hydroysteroid dehydrogenases). The n-3 FAs exerted an anti-oxidant effect, decreasing the production of thiobarbituric-acid reactive substances (TBARS) and inhibiting phospholipase A2 activity. 20:4n-6 (ARA), completely and 20:3n-6 partially, normalized the biosynthesis of testosterone in DMT-treated cultures, increasing ARA content inside the mitochondria, whereas the other FAs assayed failed to restore androgenesis. COX-2 protein and prostaglandin F2alfa and E2 production were stimulated by 20:3n-6, 20:4n-6, 18:3n-3 and 20:5 n-3. COX-2 protein decreased upon addition of 22:5n-3 and 22:6n-3. StAR protein was increased by 20:4n-6 and partially by 20:3n-6, likely due to its metabolic conversion into ARA. Both FAs increased the mitochondrial cholesterol pool available for testosterone biosynthesis. The rate of androgenesis is likely the result of various regulatory factors acting concomitantly on the physiology of Leydig cells.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269437
Astiz, Mariana; Hurtado, Graciela Elisa; Tacconi, Maria Josefa; Marra, Carlos Alberto; Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells; Springer Heidelberg; Lipids; 47; 6; 4-2012; 557-569
0024-4201
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269437
identifier_str_mv Astiz, Mariana; Hurtado, Graciela Elisa; Tacconi, Maria Josefa; Marra, Carlos Alberto; Exogenous Arachidonate Restores the Dimethoate‐Induced Inhibition of Steroidogenesis in Rat Interstitial Cells; Springer Heidelberg; Lipids; 47; 6; 4-2012; 557-569
0024-4201
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11745-012-3669-y
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-012-3669-y
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Heidelberg
publisher.none.fl_str_mv Springer Heidelberg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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