Analysis of telomere length in mantle cell lymphoma

Autores
Cottliar, Alejandra S. H.; Panero, Julieta; Pedrazzini, Estela Marta; Noriega, Maria Fernanda; Narbaitz, Marina; Rodríguez, Andrea; Slavutsky, Irma Rosa
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mantle cell lymphoma (MCL) is a well defined lymphoid neoplasm genetically characterized by the t(11;14)(q13;q32). Telomeres play an essential role in preserving chromosomal integrity and genomic stability; their shortening can lead to telomere dysfunction and chromosomal instability, a critical factor in cancer development. In this study, telomere length (TL) measured by terminal restriction fragments (TRF) assay in DNA samples of tumor cells from 20 patients with MCL was evaluated. Results were correlated with clinical, morphologic and cytogenetic characteristics. In all cases, the presence of the CCND1/IGH rearrangement was confirmed by fluorescence in situ hybridization and/or PCR analysis. TL in total MCL patients revealed a mean TRF value (4.51 ± 0.79 kb) significantly shorter than those observed in controls (7.49 ± 1.94 kb) (P < 0.001); 30% of patients had TL shorter than 4.0 kb. TRF length was not associated with patients age (P = 0.07; r = 0.17) nor with sex (females: 4.33 ± 0.51 kb and males: 4.57 ± 0.85 kb; P = 0.63). No significant differences were found between patients studied at diagnosis (13) (4.44 ± 0.81 kb) respect to those analyzed at relapse (7) (4.63 ± 0.82 kb) (P = 0.53). In addition, we compared patients with (4.84 ± 1.09 kb) and without (4.40 ± 0.68 kb) complex karyotypes (P = 0.45) and cases with typical morphology (4.48 ± 0.79 kb) vs. blastoid variant (4.63 ± 1.04 kb) (P = 0.83), and no significant differences between them were found. Although the number of cases of our series is not large, our results showed that TL reduction in MCL is independent of the clinical characteristics, morphology and karyotype. © 2009 John Wiley & Sons A/S.
Fil: Cottliar, Alejandra S. H.. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Pedrazzini, Estela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Noriega, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Narbaitz, Marina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Rodríguez, Andrea. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Materia
Mantle Cell Lymphoma
Telomere Length
Terminal Restriction Fragments
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54504
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54504
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of telomere length in mantle cell lymphomaCottliar, Alejandra S. H.Panero, JulietaPedrazzini, Estela MartaNoriega, Maria FernandaNarbaitz, MarinaRodríguez, AndreaSlavutsky, Irma RosaMantle Cell LymphomaTelomere LengthTerminal Restriction Fragmentshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Mantle cell lymphoma (MCL) is a well defined lymphoid neoplasm genetically characterized by the t(11;14)(q13;q32). Telomeres play an essential role in preserving chromosomal integrity and genomic stability; their shortening can lead to telomere dysfunction and chromosomal instability, a critical factor in cancer development. In this study, telomere length (TL) measured by terminal restriction fragments (TRF) assay in DNA samples of tumor cells from 20 patients with MCL was evaluated. Results were correlated with clinical, morphologic and cytogenetic characteristics. In all cases, the presence of the CCND1/IGH rearrangement was confirmed by fluorescence in situ hybridization and/or PCR analysis. TL in total MCL patients revealed a mean TRF value (4.51 ± 0.79 kb) significantly shorter than those observed in controls (7.49 ± 1.94 kb) (P < 0.001); 30% of patients had TL shorter than 4.0 kb. TRF length was not associated with patients age (P = 0.07; r = 0.17) nor with sex (females: 4.33 ± 0.51 kb and males: 4.57 ± 0.85 kb; P = 0.63). No significant differences were found between patients studied at diagnosis (13) (4.44 ± 0.81 kb) respect to those analyzed at relapse (7) (4.63 ± 0.82 kb) (P = 0.53). In addition, we compared patients with (4.84 ± 1.09 kb) and without (4.40 ± 0.68 kb) complex karyotypes (P = 0.45) and cases with typical morphology (4.48 ± 0.79 kb) vs. blastoid variant (4.63 ± 1.04 kb) (P = 0.83), and no significant differences between them were found. Although the number of cases of our series is not large, our results showed that TL reduction in MCL is independent of the clinical characteristics, morphology and karyotype. © 2009 John Wiley & Sons A/S.Fil: Cottliar, Alejandra S. H.. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Pedrazzini, Estela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Noriega, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Narbaitz, Marina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Rodríguez, Andrea. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaWiley Blackwell Publishing, Inc2009-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54504Cottliar, Alejandra S. H.; Panero, Julieta; Pedrazzini, Estela Marta; Noriega, Maria Fernanda; Narbaitz, Marina; et al.; Analysis of telomere length in mantle cell lymphoma; Wiley Blackwell Publishing, Inc; European Journal Of Haematology; 83; 5; 11-2009; 433-4390902-4441CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-0609.2009.01313.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-0609.2009.01313.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:08:10Zoai:ri.conicet.gov.ar:11336/54504instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:08:10.409CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of telomere length in mantle cell lymphoma
title Analysis of telomere length in mantle cell lymphoma
spellingShingle Analysis of telomere length in mantle cell lymphoma
Cottliar, Alejandra S. H.
Mantle Cell Lymphoma
Telomere Length
Terminal Restriction Fragments
title_short Analysis of telomere length in mantle cell lymphoma
title_full Analysis of telomere length in mantle cell lymphoma
title_fullStr Analysis of telomere length in mantle cell lymphoma
title_full_unstemmed Analysis of telomere length in mantle cell lymphoma
title_sort Analysis of telomere length in mantle cell lymphoma
dc.creator.none.fl_str_mv Cottliar, Alejandra S. H.
Panero, Julieta
Pedrazzini, Estela Marta
Noriega, Maria Fernanda
Narbaitz, Marina
Rodríguez, Andrea
Slavutsky, Irma Rosa
author Cottliar, Alejandra S. H.
author_facet Cottliar, Alejandra S. H.
Panero, Julieta
Pedrazzini, Estela Marta
Noriega, Maria Fernanda
Narbaitz, Marina
Rodríguez, Andrea
Slavutsky, Irma Rosa
author_role author
author2 Panero, Julieta
Pedrazzini, Estela Marta
Noriega, Maria Fernanda
Narbaitz, Marina
Rodríguez, Andrea
Slavutsky, Irma Rosa
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Mantle Cell Lymphoma
Telomere Length
Terminal Restriction Fragments
topic Mantle Cell Lymphoma
Telomere Length
Terminal Restriction Fragments
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Mantle cell lymphoma (MCL) is a well defined lymphoid neoplasm genetically characterized by the t(11;14)(q13;q32). Telomeres play an essential role in preserving chromosomal integrity and genomic stability; their shortening can lead to telomere dysfunction and chromosomal instability, a critical factor in cancer development. In this study, telomere length (TL) measured by terminal restriction fragments (TRF) assay in DNA samples of tumor cells from 20 patients with MCL was evaluated. Results were correlated with clinical, morphologic and cytogenetic characteristics. In all cases, the presence of the CCND1/IGH rearrangement was confirmed by fluorescence in situ hybridization and/or PCR analysis. TL in total MCL patients revealed a mean TRF value (4.51 ± 0.79 kb) significantly shorter than those observed in controls (7.49 ± 1.94 kb) (P < 0.001); 30% of patients had TL shorter than 4.0 kb. TRF length was not associated with patients age (P = 0.07; r = 0.17) nor with sex (females: 4.33 ± 0.51 kb and males: 4.57 ± 0.85 kb; P = 0.63). No significant differences were found between patients studied at diagnosis (13) (4.44 ± 0.81 kb) respect to those analyzed at relapse (7) (4.63 ± 0.82 kb) (P = 0.53). In addition, we compared patients with (4.84 ± 1.09 kb) and without (4.40 ± 0.68 kb) complex karyotypes (P = 0.45) and cases with typical morphology (4.48 ± 0.79 kb) vs. blastoid variant (4.63 ± 1.04 kb) (P = 0.83), and no significant differences between them were found. Although the number of cases of our series is not large, our results showed that TL reduction in MCL is independent of the clinical characteristics, morphology and karyotype. © 2009 John Wiley & Sons A/S.
Fil: Cottliar, Alejandra S. H.. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Pedrazzini, Estela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Noriega, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Narbaitz, Marina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Rodríguez, Andrea. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
description Mantle cell lymphoma (MCL) is a well defined lymphoid neoplasm genetically characterized by the t(11;14)(q13;q32). Telomeres play an essential role in preserving chromosomal integrity and genomic stability; their shortening can lead to telomere dysfunction and chromosomal instability, a critical factor in cancer development. In this study, telomere length (TL) measured by terminal restriction fragments (TRF) assay in DNA samples of tumor cells from 20 patients with MCL was evaluated. Results were correlated with clinical, morphologic and cytogenetic characteristics. In all cases, the presence of the CCND1/IGH rearrangement was confirmed by fluorescence in situ hybridization and/or PCR analysis. TL in total MCL patients revealed a mean TRF value (4.51 ± 0.79 kb) significantly shorter than those observed in controls (7.49 ± 1.94 kb) (P < 0.001); 30% of patients had TL shorter than 4.0 kb. TRF length was not associated with patients age (P = 0.07; r = 0.17) nor with sex (females: 4.33 ± 0.51 kb and males: 4.57 ± 0.85 kb; P = 0.63). No significant differences were found between patients studied at diagnosis (13) (4.44 ± 0.81 kb) respect to those analyzed at relapse (7) (4.63 ± 0.82 kb) (P = 0.53). In addition, we compared patients with (4.84 ± 1.09 kb) and without (4.40 ± 0.68 kb) complex karyotypes (P = 0.45) and cases with typical morphology (4.48 ± 0.79 kb) vs. blastoid variant (4.63 ± 1.04 kb) (P = 0.83), and no significant differences between them were found. Although the number of cases of our series is not large, our results showed that TL reduction in MCL is independent of the clinical characteristics, morphology and karyotype. © 2009 John Wiley & Sons A/S.
publishDate 2009
dc.date.none.fl_str_mv 2009-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54504
Cottliar, Alejandra S. H.; Panero, Julieta; Pedrazzini, Estela Marta; Noriega, Maria Fernanda; Narbaitz, Marina; et al.; Analysis of telomere length in mantle cell lymphoma; Wiley Blackwell Publishing, Inc; European Journal Of Haematology; 83; 5; 11-2009; 433-439
0902-4441
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54504
identifier_str_mv Cottliar, Alejandra S. H.; Panero, Julieta; Pedrazzini, Estela Marta; Noriega, Maria Fernanda; Narbaitz, Marina; et al.; Analysis of telomere length in mantle cell lymphoma; Wiley Blackwell Publishing, Inc; European Journal Of Haematology; 83; 5; 11-2009; 433-439
0902-4441
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-0609.2009.01313.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-0609.2009.01313.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.120347

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