A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases

Autores
Lu, Stephen; da Rocha, Leticia A.; Torquato, Ricardo J.S.; da Silva Vaz Junior, Itabajara; Jacobsen, Monica Ofelia; Tanaka, Aparecida S.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rhipicephalus microplus is a cattle ectoparasite found in tropical and subtropical regions around the world with great impact on livestock production. R. microplus can also harbor pathogens, such as Babesia sp. and Anaplasma sp. which further compromise cattle production. Blood meal acquisition and digestion are key steps for tick development. In ticks, digestion takes place inside midgut cells and is mediated by aspartic and cysteine peptidases and, therefore, regulated by their inhibitors. Cystatins are a family of cysteine peptidases inhibitors found in several organisms and have been associated in ticks with blood acquisition, blood digestion, modulation of host immune response and tick immunity. In this work, we characterized a novel R. microplus type 1 cystatin, named Rmcystatin-1b. The inhibitor transcripts were found to be highly expressed in the midgut of partially and fully engorged females and they appear to be modulated at different days post-detachment. Purified recombinant Rmcystatin-1b displayed inhibitory activity towards typical cysteine peptidases with high affinity. Moreover, rRmcystatin-1b was able to inhibit native R. microplus cysteine peptidases and RNAi-mediated knockdown of the cystatin transcripts resulted in increased proteolytic activity. Moreover, rRmcystatin-1b was able to interfere with B. bovis growth in vitro. Taken together our data strongly suggest that Rmcystatin-1b is a regulator of blood digestion in R. microplus midgut.
Fil: Lu, Stephen. Universidade Federal de Sao Paulo; Brasil
Fil: da Rocha, Leticia A.. Universidade Federal de Sao Paulo; Brasil
Fil: Torquato, Ricardo J.S.. Universidade Federal de Sao Paulo; Brasil
Fil: da Silva Vaz Junior, Itabajara. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Jacobsen, Monica Ofelia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Tanaka, Aparecida S.. Universidade Federal de Sao Paulo; Brasil
Materia
BABESIA BOVIS
CYSTATIN
CYSTEINE PROTEASES
MIDGUT
PROTEIN DIGESTION
TICK
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/144002
Acceder
id CONICETDig_33aff9d50b834edb0a38b5e22e044828
oai_identifier_str oai:ri.conicet.gov.ar:11336/144002
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteasesLu, Stephenda Rocha, Leticia A.Torquato, Ricardo J.S.da Silva Vaz Junior, ItabajaraJacobsen, Monica OfeliaTanaka, Aparecida S.BABESIA BOVISCYSTATINCYSTEINE PROTEASESMIDGUTPROTEIN DIGESTIONTICKhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rhipicephalus microplus is a cattle ectoparasite found in tropical and subtropical regions around the world with great impact on livestock production. R. microplus can also harbor pathogens, such as Babesia sp. and Anaplasma sp. which further compromise cattle production. Blood meal acquisition and digestion are key steps for tick development. In ticks, digestion takes place inside midgut cells and is mediated by aspartic and cysteine peptidases and, therefore, regulated by their inhibitors. Cystatins are a family of cysteine peptidases inhibitors found in several organisms and have been associated in ticks with blood acquisition, blood digestion, modulation of host immune response and tick immunity. In this work, we characterized a novel R. microplus type 1 cystatin, named Rmcystatin-1b. The inhibitor transcripts were found to be highly expressed in the midgut of partially and fully engorged females and they appear to be modulated at different days post-detachment. Purified recombinant Rmcystatin-1b displayed inhibitory activity towards typical cysteine peptidases with high affinity. Moreover, rRmcystatin-1b was able to inhibit native R. microplus cysteine peptidases and RNAi-mediated knockdown of the cystatin transcripts resulted in increased proteolytic activity. Moreover, rRmcystatin-1b was able to interfere with B. bovis growth in vitro. Taken together our data strongly suggest that Rmcystatin-1b is a regulator of blood digestion in R. microplus midgut.Fil: Lu, Stephen. Universidade Federal de Sao Paulo; BrasilFil: da Rocha, Leticia A.. Universidade Federal de Sao Paulo; BrasilFil: Torquato, Ricardo J.S.. Universidade Federal de Sao Paulo; BrasilFil: da Silva Vaz Junior, Itabajara. Universidade Federal do Rio Grande do Sul; BrasilFil: Jacobsen, Monica Ofelia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; ArgentinaFil: Tanaka, Aparecida S.. Universidade Federal de Sao Paulo; BrasilElsevier2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/144002Lu, Stephen; da Rocha, Leticia A.; Torquato, Ricardo J.S.; da Silva Vaz Junior, Itabajara; Jacobsen, Monica Ofelia; et al.; A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases; Elsevier; Ticks and Tick-borne Diseases; 11; 3; 5-2020; 1-291877-959XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1877959X19304042info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ttbdis.2020.101374info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:18:49Zoai:ri.conicet.gov.ar:11336/144002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:18:49.762CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases
title A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases
spellingShingle A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases
Lu, Stephen
BABESIA BOVIS
CYSTATIN
CYSTEINE PROTEASES
MIDGUT
PROTEIN DIGESTION
TICK
title_short A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases
title_full A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases
title_fullStr A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases
title_full_unstemmed A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases
title_sort A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases
dc.creator.none.fl_str_mv Lu, Stephen
da Rocha, Leticia A.
Torquato, Ricardo J.S.
da Silva Vaz Junior, Itabajara
Jacobsen, Monica Ofelia
Tanaka, Aparecida S.
author Lu, Stephen
author_facet Lu, Stephen
da Rocha, Leticia A.
Torquato, Ricardo J.S.
da Silva Vaz Junior, Itabajara
Jacobsen, Monica Ofelia
Tanaka, Aparecida S.
author_role author
author2 da Rocha, Leticia A.
Torquato, Ricardo J.S.
da Silva Vaz Junior, Itabajara
Jacobsen, Monica Ofelia
Tanaka, Aparecida S.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BABESIA BOVIS
CYSTATIN
CYSTEINE PROTEASES
MIDGUT
PROTEIN DIGESTION
TICK
topic BABESIA BOVIS
CYSTATIN
CYSTEINE PROTEASES
MIDGUT
PROTEIN DIGESTION
TICK
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rhipicephalus microplus is a cattle ectoparasite found in tropical and subtropical regions around the world with great impact on livestock production. R. microplus can also harbor pathogens, such as Babesia sp. and Anaplasma sp. which further compromise cattle production. Blood meal acquisition and digestion are key steps for tick development. In ticks, digestion takes place inside midgut cells and is mediated by aspartic and cysteine peptidases and, therefore, regulated by their inhibitors. Cystatins are a family of cysteine peptidases inhibitors found in several organisms and have been associated in ticks with blood acquisition, blood digestion, modulation of host immune response and tick immunity. In this work, we characterized a novel R. microplus type 1 cystatin, named Rmcystatin-1b. The inhibitor transcripts were found to be highly expressed in the midgut of partially and fully engorged females and they appear to be modulated at different days post-detachment. Purified recombinant Rmcystatin-1b displayed inhibitory activity towards typical cysteine peptidases with high affinity. Moreover, rRmcystatin-1b was able to inhibit native R. microplus cysteine peptidases and RNAi-mediated knockdown of the cystatin transcripts resulted in increased proteolytic activity. Moreover, rRmcystatin-1b was able to interfere with B. bovis growth in vitro. Taken together our data strongly suggest that Rmcystatin-1b is a regulator of blood digestion in R. microplus midgut.
Fil: Lu, Stephen. Universidade Federal de Sao Paulo; Brasil
Fil: da Rocha, Leticia A.. Universidade Federal de Sao Paulo; Brasil
Fil: Torquato, Ricardo J.S.. Universidade Federal de Sao Paulo; Brasil
Fil: da Silva Vaz Junior, Itabajara. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Jacobsen, Monica Ofelia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Tanaka, Aparecida S.. Universidade Federal de Sao Paulo; Brasil
description Rhipicephalus microplus is a cattle ectoparasite found in tropical and subtropical regions around the world with great impact on livestock production. R. microplus can also harbor pathogens, such as Babesia sp. and Anaplasma sp. which further compromise cattle production. Blood meal acquisition and digestion are key steps for tick development. In ticks, digestion takes place inside midgut cells and is mediated by aspartic and cysteine peptidases and, therefore, regulated by their inhibitors. Cystatins are a family of cysteine peptidases inhibitors found in several organisms and have been associated in ticks with blood acquisition, blood digestion, modulation of host immune response and tick immunity. In this work, we characterized a novel R. microplus type 1 cystatin, named Rmcystatin-1b. The inhibitor transcripts were found to be highly expressed in the midgut of partially and fully engorged females and they appear to be modulated at different days post-detachment. Purified recombinant Rmcystatin-1b displayed inhibitory activity towards typical cysteine peptidases with high affinity. Moreover, rRmcystatin-1b was able to inhibit native R. microplus cysteine peptidases and RNAi-mediated knockdown of the cystatin transcripts resulted in increased proteolytic activity. Moreover, rRmcystatin-1b was able to interfere with B. bovis growth in vitro. Taken together our data strongly suggest that Rmcystatin-1b is a regulator of blood digestion in R. microplus midgut.
publishDate 2020
dc.date.none.fl_str_mv 2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/144002
Lu, Stephen; da Rocha, Leticia A.; Torquato, Ricardo J.S.; da Silva Vaz Junior, Itabajara; Jacobsen, Monica Ofelia; et al.; A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases; Elsevier; Ticks and Tick-borne Diseases; 11; 3; 5-2020; 1-29
1877-959X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/144002
identifier_str_mv Lu, Stephen; da Rocha, Leticia A.; Torquato, Ricardo J.S.; da Silva Vaz Junior, Itabajara; Jacobsen, Monica Ofelia; et al.; A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases; Elsevier; Ticks and Tick-borne Diseases; 11; 3; 5-2020; 1-29
1877-959X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1877959X19304042
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ttbdis.2020.101374
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981026153889792
score 12.48226

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