Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina)

Autores
de la Barra, Paula María; Giménez, Juliana; Knack de Almeida, Henrique; Arrighetti, Florencia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The reproductive cycle of Trophon geversianus (Pallas 1774) population from Golfo San Jose´ (Chubut, Argentina, 42330 S, 64330 W) was studied using histochemical methods and digital image analysis. For such purpose, ovary samples were taken monthly between July 2006 and August 2007 and stained with hematoxylin and eosin, Sudan black B (to identify lipids) or Schiff’s ninhydrin (to identify proteins). Four different gametogenic stages were described: oogonias, previtellogenic oocytes, early vitellogenic oocytes, and late vitellogenic oocytes. Two spawning events were registered; one between September and October 2006, and a second one between February and March 2007. Oocyte quality was determined by the changes in lipid and protein composition during gametogenesis. Through digital image analysis, a lipid and a protein indexes (LI and PI) were calculated. Significant differences were observed in LI over month sampled, contrarily to what PI showed. A significant and positive correlation was found between lipid and protein content and oocytes areas, indicating that these nutrients accumulate during the entire vitellogenesis. Also, when dividing the oocytes into two size groups, analysis indicates a positive correlation between LI and oocyte area for smaller area oocytes. This demonstrates that while proteins accumulate linearly throughout vitellogenesis process, lipids accumulate in two steps: first at a growing rate, secondly at constant rate. This information is essential to determine the nutritional requirements of brood stock individuals at hatcheries in this potential fishery resource that inhabits patagonian waters.
Fil: de la Barra, Paula María. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giménez, Juliana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Knack de Almeida, Henrique. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arrighetti, Florencia. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Patagonian Gastropod
Oocyte Quality
Reproduction
Histochemistry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16910
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina)de la Barra, Paula MaríaGiménez, JulianaKnack de Almeida, HenriqueArrighetti, FlorenciaPatagonian GastropodOocyte QualityReproductionHistochemistryhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The reproductive cycle of Trophon geversianus (Pallas 1774) population from Golfo San Jose´ (Chubut, Argentina, 42330 S, 64330 W) was studied using histochemical methods and digital image analysis. For such purpose, ovary samples were taken monthly between July 2006 and August 2007 and stained with hematoxylin and eosin, Sudan black B (to identify lipids) or Schiff’s ninhydrin (to identify proteins). Four different gametogenic stages were described: oogonias, previtellogenic oocytes, early vitellogenic oocytes, and late vitellogenic oocytes. Two spawning events were registered; one between September and October 2006, and a second one between February and March 2007. Oocyte quality was determined by the changes in lipid and protein composition during gametogenesis. Through digital image analysis, a lipid and a protein indexes (LI and PI) were calculated. Significant differences were observed in LI over month sampled, contrarily to what PI showed. A significant and positive correlation was found between lipid and protein content and oocytes areas, indicating that these nutrients accumulate during the entire vitellogenesis. Also, when dividing the oocytes into two size groups, analysis indicates a positive correlation between LI and oocyte area for smaller area oocytes. This demonstrates that while proteins accumulate linearly throughout vitellogenesis process, lipids accumulate in two steps: first at a growing rate, secondly at constant rate. This information is essential to determine the nutritional requirements of brood stock individuals at hatcheries in this potential fishery resource that inhabits patagonian waters.Fil: de la Barra, Paula María. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Giménez, Juliana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Knack de Almeida, Henrique. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arrighetti, Florencia. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16910de la Barra, Paula María; Giménez, Juliana; Knack de Almeida, Henrique; Arrighetti, Florencia; Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina); Springer; Helgoland Marine Research; 68; 1; 3-2014; 179-1851438-387X1438-3888enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10152-014-0380-9info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10152-014-0380-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:06:21Zoai:ri.conicet.gov.ar:11336/16910instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:06:21.246CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina)
title Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina)
spellingShingle Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina)
de la Barra, Paula María
Patagonian Gastropod
Oocyte Quality
Reproduction
Histochemistry
title_short Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina)
title_full Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina)
title_fullStr Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina)
title_full_unstemmed Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina)
title_sort Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina)
dc.creator.none.fl_str_mv de la Barra, Paula María
Giménez, Juliana
Knack de Almeida, Henrique
Arrighetti, Florencia
author de la Barra, Paula María
author_facet de la Barra, Paula María
Giménez, Juliana
Knack de Almeida, Henrique
Arrighetti, Florencia
author_role author
author2 Giménez, Juliana
Knack de Almeida, Henrique
Arrighetti, Florencia
author2_role author
author
author
dc.subject.none.fl_str_mv Patagonian Gastropod
Oocyte Quality
Reproduction
Histochemistry
topic Patagonian Gastropod
Oocyte Quality
Reproduction
Histochemistry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The reproductive cycle of Trophon geversianus (Pallas 1774) population from Golfo San Jose´ (Chubut, Argentina, 42330 S, 64330 W) was studied using histochemical methods and digital image analysis. For such purpose, ovary samples were taken monthly between July 2006 and August 2007 and stained with hematoxylin and eosin, Sudan black B (to identify lipids) or Schiff’s ninhydrin (to identify proteins). Four different gametogenic stages were described: oogonias, previtellogenic oocytes, early vitellogenic oocytes, and late vitellogenic oocytes. Two spawning events were registered; one between September and October 2006, and a second one between February and March 2007. Oocyte quality was determined by the changes in lipid and protein composition during gametogenesis. Through digital image analysis, a lipid and a protein indexes (LI and PI) were calculated. Significant differences were observed in LI over month sampled, contrarily to what PI showed. A significant and positive correlation was found between lipid and protein content and oocytes areas, indicating that these nutrients accumulate during the entire vitellogenesis. Also, when dividing the oocytes into two size groups, analysis indicates a positive correlation between LI and oocyte area for smaller area oocytes. This demonstrates that while proteins accumulate linearly throughout vitellogenesis process, lipids accumulate in two steps: first at a growing rate, secondly at constant rate. This information is essential to determine the nutritional requirements of brood stock individuals at hatcheries in this potential fishery resource that inhabits patagonian waters.
Fil: de la Barra, Paula María. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giménez, Juliana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Knack de Almeida, Henrique. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arrighetti, Florencia. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Biología de Invertebrados Marinos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The reproductive cycle of Trophon geversianus (Pallas 1774) population from Golfo San Jose´ (Chubut, Argentina, 42330 S, 64330 W) was studied using histochemical methods and digital image analysis. For such purpose, ovary samples were taken monthly between July 2006 and August 2007 and stained with hematoxylin and eosin, Sudan black B (to identify lipids) or Schiff’s ninhydrin (to identify proteins). Four different gametogenic stages were described: oogonias, previtellogenic oocytes, early vitellogenic oocytes, and late vitellogenic oocytes. Two spawning events were registered; one between September and October 2006, and a second one between February and March 2007. Oocyte quality was determined by the changes in lipid and protein composition during gametogenesis. Through digital image analysis, a lipid and a protein indexes (LI and PI) were calculated. Significant differences were observed in LI over month sampled, contrarily to what PI showed. A significant and positive correlation was found between lipid and protein content and oocytes areas, indicating that these nutrients accumulate during the entire vitellogenesis. Also, when dividing the oocytes into two size groups, analysis indicates a positive correlation between LI and oocyte area for smaller area oocytes. This demonstrates that while proteins accumulate linearly throughout vitellogenesis process, lipids accumulate in two steps: first at a growing rate, secondly at constant rate. This information is essential to determine the nutritional requirements of brood stock individuals at hatcheries in this potential fishery resource that inhabits patagonian waters.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16910
de la Barra, Paula María; Giménez, Juliana; Knack de Almeida, Henrique; Arrighetti, Florencia; Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina); Springer; Helgoland Marine Research; 68; 1; 3-2014; 179-185
1438-387X
1438-3888
url http://hdl.handle.net/11336/16910
identifier_str_mv de la Barra, Paula María; Giménez, Juliana; Knack de Almeida, Henrique; Arrighetti, Florencia; Vitellogenesis and changes in lipid and protein content of oocytes of Trophon geversianus (Neogastropoda: Muricidae) in Golfo San José (Chubut, Argentina); Springer; Helgoland Marine Research; 68; 1; 3-2014; 179-185
1438-387X
1438-3888
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10152-014-0380-9
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10152-014-0380-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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