Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions

Autores
Mayr, Christoph; Massaferro, Julieta; Wissel, Holger; Lücke, Andreas
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oxygen isotopes of chironomid head capsules in lake sediments have become an important proxy for climatereconstructions, especially for high-latitude regions. Various chemical pretreatments have been developed toremove organic and inorganic contaminants and to clean the head capsules prior to isotope analysis, but so farthere is no standardised routine method to assess the purity of the isolated chitin. We present a dataset of oxygenisotope analyses of head capsules from seven lakes in southern South America. The head capsules were assignedto three different taxonomic groups of Chironomidae (Chironominae, Orthocladiinae, Tanypodinae) and toCeratopogonidae. Orthocladiinae exhibited on average higher isotopic fractionation relative to lake water thanthe other taxonomic groups, although these differences were not statistically significant. Elemental analysesdemonstrate that the oxygen content and molar N/O ratio of the head capsules have a larger influence on oxygenisotopic composition than the host water isotopic composition suggesting a strong influence of incompletedeproteination on oxygen isotope values of chironomid head capsules. This is backed up by an experimentalapproach with differently pretreated chironomids, which exhibits a strong correlation with N/O ratio presum-ably due to various degrees of deproteination. According to the N/O ratio, pure chitin was obtained after removalof the minerogenic fraction with a mixture of 10 % HF and 5 % HCl (16 h, 20 ◦C) and subsequent treatment with5 % NaOH (2 h) and 7 % NaClO2 (10 h). In conclusion, we recommend using the N/O ratio for purity control anda critical assessment of different pretreatment methods to obtain pure chitin in future studies.
Fil: Mayr, Christoph. Universitat Erlangen-Nuremberg; Alemania
Fil: Massaferro, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Wissel, Holger. Universitat Erlangen-Nuremberg; Alemania
Fil: Lücke, Andreas. Universitat Erlangen-Nuremberg; Alemania
Materia
Chironomid head capsules
oxygen isotopes
Patagonia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/273016
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructionsMayr, ChristophMassaferro, JulietaWissel, HolgerLücke, AndreasChironomid head capsulesoxygen isotopesPatagoniahttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Oxygen isotopes of chironomid head capsules in lake sediments have become an important proxy for climatereconstructions, especially for high-latitude regions. Various chemical pretreatments have been developed toremove organic and inorganic contaminants and to clean the head capsules prior to isotope analysis, but so farthere is no standardised routine method to assess the purity of the isolated chitin. We present a dataset of oxygenisotope analyses of head capsules from seven lakes in southern South America. The head capsules were assignedto three different taxonomic groups of Chironomidae (Chironominae, Orthocladiinae, Tanypodinae) and toCeratopogonidae. Orthocladiinae exhibited on average higher isotopic fractionation relative to lake water thanthe other taxonomic groups, although these differences were not statistically significant. Elemental analysesdemonstrate that the oxygen content and molar N/O ratio of the head capsules have a larger influence on oxygenisotopic composition than the host water isotopic composition suggesting a strong influence of incompletedeproteination on oxygen isotope values of chironomid head capsules. This is backed up by an experimentalapproach with differently pretreated chironomids, which exhibits a strong correlation with N/O ratio presum-ably due to various degrees of deproteination. According to the N/O ratio, pure chitin was obtained after removalof the minerogenic fraction with a mixture of 10 % HF and 5 % HCl (16 h, 20 ◦C) and subsequent treatment with5 % NaOH (2 h) and 7 % NaClO2 (10 h). In conclusion, we recommend using the N/O ratio for purity control anda critical assessment of different pretreatment methods to obtain pure chitin in future studies.Fil: Mayr, Christoph. Universitat Erlangen-Nuremberg; AlemaniaFil: Massaferro, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaFil: Wissel, Holger. Universitat Erlangen-Nuremberg; AlemaniaFil: Lücke, Andreas. Universitat Erlangen-Nuremberg; AlemaniaPergamon-Elsevier Science Ltd2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/273016Mayr, Christoph; Massaferro, Julieta; Wissel, Holger; Lücke, Andreas; Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions; Pergamon-Elsevier Science Ltd; Quaternary International; 728; 5-2025; 1-91040-6182CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1040618225001090info:eu-repo/semantics/altIdentifier/doi/10.1016/j.quaint.2025.109766info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:29:49Zoai:ri.conicet.gov.ar:11336/273016instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:29:49.786CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions
title Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions
spellingShingle Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions
Mayr, Christoph
Chironomid head capsules
oxygen isotopes
Patagonia
title_short Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions
title_full Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions
title_fullStr Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions
title_full_unstemmed Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions
title_sort Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions
dc.creator.none.fl_str_mv Mayr, Christoph
Massaferro, Julieta
Wissel, Holger
Lücke, Andreas
author Mayr, Christoph
author_facet Mayr, Christoph
Massaferro, Julieta
Wissel, Holger
Lücke, Andreas
author_role author
author2 Massaferro, Julieta
Wissel, Holger
Lücke, Andreas
author2_role author
author
author
dc.subject.none.fl_str_mv Chironomid head capsules
oxygen isotopes
Patagonia
topic Chironomid head capsules
oxygen isotopes
Patagonia
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Oxygen isotopes of chironomid head capsules in lake sediments have become an important proxy for climatereconstructions, especially for high-latitude regions. Various chemical pretreatments have been developed toremove organic and inorganic contaminants and to clean the head capsules prior to isotope analysis, but so farthere is no standardised routine method to assess the purity of the isolated chitin. We present a dataset of oxygenisotope analyses of head capsules from seven lakes in southern South America. The head capsules were assignedto three different taxonomic groups of Chironomidae (Chironominae, Orthocladiinae, Tanypodinae) and toCeratopogonidae. Orthocladiinae exhibited on average higher isotopic fractionation relative to lake water thanthe other taxonomic groups, although these differences were not statistically significant. Elemental analysesdemonstrate that the oxygen content and molar N/O ratio of the head capsules have a larger influence on oxygenisotopic composition than the host water isotopic composition suggesting a strong influence of incompletedeproteination on oxygen isotope values of chironomid head capsules. This is backed up by an experimentalapproach with differently pretreated chironomids, which exhibits a strong correlation with N/O ratio presum-ably due to various degrees of deproteination. According to the N/O ratio, pure chitin was obtained after removalof the minerogenic fraction with a mixture of 10 % HF and 5 % HCl (16 h, 20 ◦C) and subsequent treatment with5 % NaOH (2 h) and 7 % NaClO2 (10 h). In conclusion, we recommend using the N/O ratio for purity control anda critical assessment of different pretreatment methods to obtain pure chitin in future studies.
Fil: Mayr, Christoph. Universitat Erlangen-Nuremberg; Alemania
Fil: Massaferro, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Wissel, Holger. Universitat Erlangen-Nuremberg; Alemania
Fil: Lücke, Andreas. Universitat Erlangen-Nuremberg; Alemania
description Oxygen isotopes of chironomid head capsules in lake sediments have become an important proxy for climatereconstructions, especially for high-latitude regions. Various chemical pretreatments have been developed toremove organic and inorganic contaminants and to clean the head capsules prior to isotope analysis, but so farthere is no standardised routine method to assess the purity of the isolated chitin. We present a dataset of oxygenisotope analyses of head capsules from seven lakes in southern South America. The head capsules were assignedto three different taxonomic groups of Chironomidae (Chironominae, Orthocladiinae, Tanypodinae) and toCeratopogonidae. Orthocladiinae exhibited on average higher isotopic fractionation relative to lake water thanthe other taxonomic groups, although these differences were not statistically significant. Elemental analysesdemonstrate that the oxygen content and molar N/O ratio of the head capsules have a larger influence on oxygenisotopic composition than the host water isotopic composition suggesting a strong influence of incompletedeproteination on oxygen isotope values of chironomid head capsules. This is backed up by an experimentalapproach with differently pretreated chironomids, which exhibits a strong correlation with N/O ratio presum-ably due to various degrees of deproteination. According to the N/O ratio, pure chitin was obtained after removalof the minerogenic fraction with a mixture of 10 % HF and 5 % HCl (16 h, 20 ◦C) and subsequent treatment with5 % NaOH (2 h) and 7 % NaClO2 (10 h). In conclusion, we recommend using the N/O ratio for purity control anda critical assessment of different pretreatment methods to obtain pure chitin in future studies.
publishDate 2025
dc.date.none.fl_str_mv 2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/273016
Mayr, Christoph; Massaferro, Julieta; Wissel, Holger; Lücke, Andreas; Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions; Pergamon-Elsevier Science Ltd; Quaternary International; 728; 5-2025; 1-9
1040-6182
CONICET Digital
CONICET
url http://hdl.handle.net/11336/273016
identifier_str_mv Mayr, Christoph; Massaferro, Julieta; Wissel, Holger; Lücke, Andreas; Chemical composition influences δ18O of chironomid larvae head capsules - Implications for climate reconstructions; Pergamon-Elsevier Science Ltd; Quaternary International; 728; 5-2025; 1-9
1040-6182
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1040618225001090
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.quaint.2025.109766
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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